Article
Cell & Tissue Engineering
Eun Wha Choi, I. -Rang Lim, Ji Hong Park, Jiwoo Song, Bongkum Choi, Sungjoo Kim
Summary: Exosomes derived from disease-condition-serum-primed iMSCs showed potential in ameliorating cartilage damage in a RA model by enhancing TGF-beta 1 production, inducing Th2 and M2 polarization, and lowering proinflammatory cytokines levels. Patient-derived serum could be a valuable priming strategy for iMSC-derived exosome treatment of RA.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Honorata Kraskiewicz, Piotr Hinc, Agnieszka Krawczenko, Aleksandra Bielawska-Pohl, Maria Paprocka, Danuta Witkowska, Isma Liza Mohd Isa, Abhay Pandit, Aleksandra Klimczak
Summary: A potential treatment for chronic wounds using a hydrogel loaded with secretome from adipose tissue mesenchymal stem cells has been proposed. The biocompatibility and pro-angiogenic activity of the hydrogel-released factors were investigated, showing positive effects on skin-origin cell proliferation and endothelial cell angiogenic properties. Additionally, antimicrobial activity was observed in the secretome, indicating its potential use as an advanced wound-healing dressing in combination with the hydrogel.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Yong-Hong Wang, Ya-Chao Tao, Dong-Bo Wu, Meng-Lan Wang, Hong Tang, En-Qiang Chen
Summary: Different storage solutions did not significantly affect cell behavior, while heterogeneity was prevalent in MSC populations and could limit cell application. Therefore, establishing a more precise standard for culture-expanded MSCs is necessary.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Cell Biology
Yulia Suzdaltseva, Kirill Goryunov, Ekaterina Silina, Natalia Manturova, Victor Stupin, Sergey L. Kiselev
Summary: Mesenchymal stem cells (MSCs) have multiple paracrine and immunomodulatory properties, providing protection from chronic inflammation and promoting tissue repair. The interaction between MSCs and immune cells can result in immunosuppression, inhibiting the proliferation of peripheral blood mononuclear cells (PBMCs). Direct cell-cell contact between MSCs and PBMCs plays a role in the regulation of IDO, IL-1, IL-6, and G-CSF.
Article
Cell Biology
Xiaoyun Wu, Yongxu Mu, Jingyi Yao, Fuhong Lin, Daocheng Wu, Zhijie Ma
Summary: The study revealed that ADSCs from UC patients exhibited defective immunosuppression, indicating their unsuitability for autologous transplantation in treating UC.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Xiaoying Jin, Yangyang Dai, Liaobing Xin, Zi Ye, Jiayu Chen, Qianhong He, Xin Chen, Xiaodong Xu, Guanghui Song, Xiaohua Yu, Songying Zhang
Summary: Endometrial injury can lead to infertility, and traditional treatments are limited in their effectiveness. This study presents a novel approach using ECM@ADSC-exos to promote endometrium regeneration. In vitro experiments showed improved cell proliferation, migration, and angiogenesis. In a rat model, ECM@ADSC-exos enhanced endometrium regeneration, promoted myometrium repair, and preserved fertility.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Dong-Sik Chae, Seongho Han, Min-Kyung Lee, Sung-Whan Kim
Summary: This study aimed to analyze the role of genome-edited BMP-2 overexpressing amniotic mesenchymal stem cells (AMMs) in a mouse model of collagen-induced arthritis (CIA). The injection of AMM/B attenuated CIA progression, inhibited Th17 cell activation, increased proteoglycan expression in cartilage, and decreased the infiltration of inflammatory cells and factors in the joint tissues. Therefore, editing the BMP-2 genome in MSCs could enhance their therapeutic potential for treating cartilage degeneration in arthritic joints.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dong-Sik Chae, Young-Jin Park, Sung-Whan Kim
Summary: IL-10-overexpressing human amniotic mesenchymal stem cells (AMM/I) generated via gene editing can be a novel therapeutic strategy for the treatment of arthritis. Transplantation of AMM/I attenuates arthritis progression, increases regulatory T cell population, and suppresses T helper-17 cell activation. In addition, AMM/I injection increases proteoglycan expression, while reducing inflammation and the expression levels of pro-inflammatory factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Polymer Science
Marfa N. Egorikhina, Irina I. Bronnikova, Yulia P. Rubtsova, Irina N. Charykova, Marina L. Bugrova, Daria D. Linkova, Diana Ya Aleynik
Summary: The success of tissue regeneration through the implantation of scaffolds or tissue-engineered structures depends on the biodegradability of the materials used. Factors such as the source of collagen and the presence of stem cells have significant effects on the biodegradation and structural properties of the scaffolds. Additionally, the pre-cultivation period for cellular scaffolds is found to influence the speed and extent of their subsequent biodegradation.
Article
Chemistry, Multidisciplinary
Shufang Shen, Huaxing Dai, Ziying Fei, Yu Chai, Yu Hao, Qin Fan, Ziliang Dong, Yujie Zhu, Jialu Xu, Qingle Ma, Xiao Han, Ligeng Xu, Fei Peng, Zhuang Liu, Chao Wang
Summary: The study developed immunosuppressive nanoparticles by coating PD-L1 overexpressed MSCs plasma membrane on PLGA nanoparticles, aiming to reduce irAEs caused by immune checkpoint inhibitors. These nanoparticles showed potential in rescuing hepatitis in a clinically relevant mouse model without affecting the efficiency of ICB therapy in inhibiting tumor growth.
Article
Biochemistry & Molecular Biology
Alexandra Damerau, Moritz Pfeiffenberger, Marie-Christin Weber, Gerd-Rudiger Burmester, Frank Buttgereit, Timo Gaber, Annemarie Lang
Summary: This study developed a human in vitro 3D osteochondral tissue model (OTM) to mimic key features of cartilage and subchondral bone destruction in rheumatoid arthritis (RA). By characterizing the model comprehensively and evaluating drug responses, it was demonstrated that the OTM can serve as a preclinical tool for assessing potential drugs in a translational setup.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Joo Youn Oh, Hyemee Kim, Hyun Ju Lee, Kangin Lee, Heather Barreda, Hyeon Ji Kim, Eunji Shin, Eun-Hye Bae, Gagandeep Kaur, Yu Zhang, Eunjae Kim, Jae Young Lee, Ryang Hwa Lee
Summary: This study found that inhibiting MHC class I in MSCs reduces their survival rate and immunosuppressive potency, while increasing their susceptibility to NK-cell-mediated cytotoxicity. There is a significant correlation between MSC survival rate and susceptibility to NK cells, as well as the potency of MSCs to increase MHC class I level upon stimulation.
Article
Biotechnology & Applied Microbiology
Xiaoyin Liu, Guijun Zhang, Pan Wei, Lifang Hao, Lin Zhong, Kunhon Zhong, Chang Liu, Peng Liu, Qingbo Feng, Shan Wang, Jianyong Zhang, Rui Tian, Liangxue Zhou
Summary: This study prepared a 3D-printed collagen/silk fibroin/secretome scaffold derived from human umbilical cord blood mesenchymal stem cells (HUCMSCs) pretreated with bFGF at a low temperature, aiming to promote brain tissue regeneration after traumatic brain injury (TBI). In vitro experiments showed that the 3D-CS-bFGF-ST scaffold exhibited good biodegradation, suitable mechanical properties, and biocompatibility. In vivo experiments demonstrated that the 3D-CS-bFGF-ST group had significantly faster brain tissue regeneration compared to the other two groups, as evidenced by motor assay, histological analysis, and immunofluorescence assay. The 3D-CS-bFGF-ST group achieved a better outcome than the 3D-CS-ST group at 6 months postsurgery.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell & Tissue Engineering
Catalina-Iolanda Marinescu, Mihai Bogdan Preda, Alexandrina Burlacu
Summary: The study presented a simple and reliable method to evaluate the immunosuppressive effect of mouse bone marrow-derived MSC, showing that MSC have a dose-dependent inhibitory effect on T cell proliferation. Variability between different batches of MSC can be determined through experiments.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Zheng Kou, Balun Li, Aili Aierken, Ning Tan, Chenchen Li, Miao Han, Yuanxiang Jing, Na Li, Shiqiang Zhang, Sha Peng, Xianjun Zhao, Jinlian Hua
Summary: The existing treatment modalities for skin injuries, such as dressings, negative-pressure wound treatment, autologous skin grafting, and high-pressure wound treatment, have limitations such as high time cost and inability to remove inactivated tissue in a timely manner. Mesenchymal stem cells (MSCs) have great potential in cell therapy and regenerative medicine due to their unique self-renewal ability and differentiation potential. The addition of collagen to cell cultures promotes cell proliferation and accelerates the healing of skin wounds in mice and canines by promoting epidermal layer repair, collagen deposition, hair follicle angiogenesis, and an inflammatory response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Md Nahidul Islam, Tomas P. Griffin, Robert Whiriskey, Siobhan Hamon, Brendan Cleary, Liam Blake, Damian G. Griffin, Matthew D. Griffin, Janusz Krawczyk, Paula M. O'Shea
Summary: The study aimed to define reference intervals for frequently requested biochemical/haematological parameters in a healthy adult Irish Caucasian population. Results showed that the majority of parameters' reference intervals were broadly in accord with existing standards, but there was a significant difference in the established interval for HbA(1c) compared to national guidelines.
IRISH JOURNAL OF MEDICAL SCIENCE
(2022)
Article
Microbiology
Pietro Marchese, Laura Garzoli, Ryan Young, Louise Allcock, Frank Barry, Maria Tuohy, Mary Murphy
Summary: This study characterizes the culturable fungal community associated with the deepest margin of the European Western Continental Shelf, revealing differences between sediment-associated and animal-associated fungal communities. Several fungal species and genera were detected for the first time in the marine environment.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Urology & Nephrology
Elhussein A. E. Elhassan, Susan L. Murray, Dervla M. Connaughton, Claire Kennedy, Sarah Cormican, Cliona Cowhig, Caragh Stapleton, Mark A. Little, Kendrah Kidd, Anthony J. Bleyer, Martina Zivna, Stanislav Kmoch, Neil K. Fennelly, Brendan Doyle, Anthony Dorman, Matthew D. Griffin, Liam Casserly, Peter C. Harris, Friedhelm Hildebrandt, Gianpiero L. Cavalleri, Katherine A. Benson, Peter J. Conlon
Summary: This study describes the clinical utility and valuable impact of a specialized clinic for genetic kidney diseases, which uses various genomic sequencing strategies for genetic testing. The clinic achieved a high molecular diagnostic rate and helped confirm or reclassify the diagnoses of patients. The implementation of this clinic has resulted in direct clinical and therapeutic benefits for affected patients.
JOURNAL OF NEPHROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Joan C. Fitzgerald, Niamh Duffy, Giacomo Cattaruzzi, Francesco Vitrani, Alice Paulitti, Flavia Mazzarol, Prisca Mauro, Antonio Sfiligoj, Francesco Curcio, Deirdre M. Jones, Veronica McInerney, Janusz Krawczyk, Jack Kelly, Andrew Finnerty, Katya McDonagh, Una McCabe, Matthew Duggan, Lauren Connolly, Georgina Shaw, Mary Murphy, Frank Barry
Summary: The study validates the utility of the NANT 001 bioreactor for the production of mesenchymal stromal cells (MSCs), showing advantages in automated production of small to medium cell batches and comparable cell yield and viability to manual processing. The automated system can achieve cell standardization, reduce labor input from operators and management personnel.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Surgery
Jesper Hjortdal, Matthew D. Griffin, Marion Cadoux, W. John Armitage, Max Bylesjo, Peadar Mac Gabhann, Conor C. Murphy, Uwe Pleyer, Derek Tole, Bertrand Vabres, Malcolm D. Walkinshaw, Pierre-Antoine Gourraud, Matilde Karakachoff, Sophie Brouard, Nicolas Degauque
Summary: This study investigated the acute rejection of corneal transplants and found that there were no activation signatures within the B cell and T cell repertoire at the time of diagnosis. However, in recipients of full-thickness corneal transplants, acute rejection was associated with differences in B cell maturity and regulatory CD4(+) T cell frequency compared to stable allografts.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Biotechnology & Applied Microbiology
Joseph A. Panos, Michael J. Coenen, Christopher V. Nagelli, Erin B. McGlinch, Aysegul Atasoy-Zeybek, Consuelo Lopez De Padilla, Ryan F. Coghlan, Brian Johnstone, Elisabeth Ferreira, Ryan M. Porter, Rodolfo E. De la Vega, Christopher H. Evans
Article
Orthopedics
T. He, C. Zhang, T. Colombani, S. A. Bencherif, R. M. Porter, A. G. Bajpayee
Summary: In this study, a multi-arm Avidin (mAv) nano-construct with high drug loading content was synthesized. The biodistribution and kinetics of mAv following intra-articular administration were investigated. The results showed that mAv could penetrate into rat cartilage and other joint tissues, making it a versatile carrier for delivering drugs to treat musculoskeletal diseases.
OSTEOARTHRITIS AND CARTILAGE
(2023)
Article
Cell & Tissue Engineering
Yolande F. M. Ramos, Tobias Tertel, Georgina Shaw, Simon Staubach, Rodrigo Coutinho de Almeida, Eka Suchiman, Thomas B. Kuipers, Hailiang Mei, Frank Barry, Mary Murphy, Bernd Giebel, Ingrid Meulenbelt
Summary: This study investigates the similarities and therapeutic potential of hiMSCs and hBMSCs. RNA sequencing reveals a high resemblance between the two, with both cell types exhibiting immunomodulatory properties. hiMSCs stand out in terms of the expression and secretion of immunomodulatory genes.
STEM CELL RESEARCH & THERAPY
(2022)
Editorial Material
Pharmacology & Pharmacy
Norberto Perico, Matthew D. Griffin, Giuseppe Remuzzi
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Federico Ferro, Renza Spelat, Georgina Shaw, Cynthia M. Coleman, Xi Zhe Chen, David Connolly, Elisabetta M. F. Palama, Chiara Gentili, Paolo Contessotto, Mary J. Murphy
Summary: This study evaluated the anti-inflammatory and regenerative effects of bone marrow MSC and their extracellular vesicles in a diabetic fractured mouse model. The results showed that preconditioned cells promoted earlier soft callus formation and mineralization at the fracture site, suggesting the importance of preconditioning in MSC therapy research and optimization.
Article
Urology & Nephrology
Norberto Perico, Giuseppe Remuzzi, Matthew D. Griffin, Paul Cockwell, Alexander P. Maxwell, Federica Casiraghi, Nadia Rubis, Tobia Peracchi, Alessandro Villa, Marta Todeschini, Fabiola Carrara, Bernadette A. Magee, Piero L. Ruggenenti, Stefano Rota, Laura Cappelletti, Veronica Mcinerney, Tomas P. Griffin, Md Nahidul Islam, Martino Introna, Olga Pedrini, Josee Golay, Andrew A. Finnerty, Jon Smythe, Willem E. Fibbe, Stephen J. Elliman, Timothy O'Brien, NEPHSTROM Trial Consortium
Summary: The NEPHSTROM study evaluates the safety, tolerability, and preliminary efficacy of ORBCEL-M cell therapy in adults with type 2 diabetes and progressive diabetic kidney disease. The results show that ORBCEL-M cell therapy is safe and well-tolerated in the lowest dose cohort, and significantly reduces the decline rate of eGFR over 18 months.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Materials Science, Biomaterials
Christina N. M. Ryan, Eugenia Pugliese, Naledi Shologu, Diana Gaspar, Peadar Rooney, Md Nahidul Islam, Alan O'Riordan, Manus J. Biggs, Matthew D. Griffin, Dimitrios I. Zeugolis
Summary: This study evaluated the effects of bidirectional surface topography, substrate rigidity, collagen type I coating, and macromolecular crowding on human bone marrow stem cell cultures. The results showed that surface topography and macromolecular crowding had significant influences on cell morphology and extracellular matrix deposition, respectively. Collagen type I coating increased cell metabolic activity. This study highlights the importance of multifactorial bioengineering in controlling cell fate.
BIOMATERIALS ADVANCES
(2023)
Article
Rheumatology
Jennifer Scott, Enock Havyarimana, Albert Navarro-Gallinad, Arthur White, Jason Wyse, Jos van Geffen, Michiel van Weele, Antonia Buettner, Tamara Wanigasekera, Cathal Walsh, Louis Aslett, John D. Kelleher, Julie Power, James Ng, Declan O'Sullivan, Lucy Hederman, Neil Basu, Mark A. Little, Lina Zgaga
Summary: This study found that low winter ambient UVB and prolonged vitamin D deficiency are associated with an increased risk of AAV relapse across all phenotypes. However, the development of a granulomatous phenotype does not appear to be directly mediated by vitamin D. Further research is needed to determine if sufficient vitamin D status could reduce relapse propensity in AAV.
ARTHRITIS RESEARCH & THERAPY
(2022)
Meeting Abstract
Endocrinology & Metabolism
Aaron Warren, Filipa Ponte, Ha-Neui Kim, Ryan Porter, Roy Morello, Maria Almeida
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Cell & Tissue Engineering
Barbara Fazekas, Senthilkumar Alagesan, Luke Watson, Olivia Ng, Callum M. Conroy, Cristina Catala, Maria Velascode Andres, Neema Negi, Jared Q. Gerlach, Sean O. Hynes, Francisco Lozano, Stephen J. Elliman, Matthew D. Griffin
Summary: This study investigated the immunomodulatory effects of a novel MSC product in mouse endotoxemia and polymicrobial sepsis models. The results showed that repeated doses of the MSC product were required to modulate immune/inflammatory events in polymicrobial sepsis and SA-AKI, while single doses had limited efficacy.
STEM CELL REVIEWS AND REPORTS
(2022)