Article
Chemistry, Medicinal
Alejandro Revuelto, Isabel Lopez-Martin, Hector de Lucio, Juan Carlos Garcia-Soriano, Nicola Zanda, Sonia de Castro, Federico Gago, Antonio Jimenez-Ruiz, Sonsoles Velazquez, Maria-Jose Camarasa
Summary: By disrupting protein-protein interactions at the dimer interface of Leishmania infantum TryR, researchers have developed a novel series of small-molecule-peptide conjugates that show good inhibitory effects on both enzyme activity and dimerization.
Article
Chemistry, Medicinal
Zhilan Zhou, Jifa Zhang, Enda Zhou, Changyu Ren, Jiaxing Wang, Yuxi Wang
Summary: HCV infection is a global health problem, and developing small-molecule inhibitors targeting NS5B RdRp is of great value. Non-nucleoside inhibitors have more flexible structures and simpler mechanisms compared to nucleoside inhibitors.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Virology
Sameera Senaweera, Haijuan Du, Huanchun Zhang, Karen A. Kirby, Philip R. Tedbury, Jiashu Xie, Stefan G. Sarafianos, Zhengqiang Wang
Summary: Novel HBV CpAM hits were identified using structure-based virtual screening and pharmacophore-guided compound design, showing potent antiviral activity and high stability in ADME assays.
Article
Immunology
Pierre Cappy, Quentin Lucas, Nakourogou Kankarafou, Camille Sureau, Syria Laperche
Summary: Following a study demonstrating the role of hepatitis C virus (HCV) in transmitting hepatitis D virus (HDV) in a liver-humanized mouse model, a large cohort of HCV-positive blood donors were screened for HDV markers. However, no evidence of HCV-assisted propagation of HDV in humans was found.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Microbiology
Bertrand Boson, Chloe Mialon, Konstanze Schichl, Solene Denolly, Francois-Loic Cosset
Summary: Nup98 is a crucial component of the nuclear pore and is also involved in annulate lamella pore structures in the cytosol. It has been found to participate in the assembly of hepatitis C virus (HCV). The study shows that during HCV infection, the core protein of HCV can relocate Nup98 from annulate lamellae to lipid droplet-apposed areas where viral assembly is promoted. Nup98 interacts with HCV RNA and its recruitment by HCV core protein to viral assembly sites increases the concentration of viral genome, facilitating its encapsidation into nascent virions.
Article
Multidisciplinary Sciences
Ja Yeon Kim Chu, Yu-Chen Chuang, Kuen-Nan Tsai, Jessica Pantuso, Yuji Ishida, Takeshi Saito, Jing-hsiung James Ou
Summary: This study reveals the association between autophagic membranes and HBV replication. The core protein is associated with phagophores, while the precore protein derivative is associated with autophagosomes. Autophagic membranes play different roles in the trafficking of core and precore proteins, as well as the assembly and release of HBV particles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Chemistry, Analytical
Vigneswaran Narayanamurthy, Z. E. Jeroish, K. S. Bhuvaneshwari, Fahmi Samsuri
Summary: Proper diagnosis is essential in avoiding disease consequences, especially for severe issues like hepatitis C virus (HCV) that can cause significant damage to the liver. Developing strategies for appropriate selection of POCT for HCV detection, initiating proper antiviral therapy, and defining associated risks is critical for achieving optimal outcomes. Alternative strategies are needed to enhance HCV diagnosis, with molecular assays being precise but requiring additional approaches for infected populations to improve detection.
ANALYTICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Alexander J. Martinko, Erin F. Simonds, Suchitra Prasad, Alberto Ponce, Colton J. Bracken, Junnian Wei, Yung-Hua Wang, Tiffany-Lynn Chow, Zhong Huang, Michael J. Evans, James A. Wells, Zachary B. Hill
Summary: Monoclonal antibodies with antigen specificity and long serum half-life are crucial in modern therapeutics. We have developed a technology for switchable assembly of functional antibody complexes using chemically induced dimerization domains, allowing control of the antibody's intended function in vivo by modulating the dose of a small molecule.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Nikesh Patel, Fardokht Abulwerdi, Farzad Fatehi, Iain W. Manfield, Stuart Le Grice, John S. Schneekloth, Reidun Twarock, Peter G. Stockley
Summary: RNA sequences/motifs dispersed across the genome of Hepatitis B Virus regulate formation of nucleocapsid-like particles by core protein in an epsilon/polymerase-independent fashion. Ligands that bind the important RNA Packaging Signals for nucleocapsid assembly were identified, and some of them showed inhibitory effects on the assembly process. While they did not inhibit HBV replication in cell culture, computational modeling suggested the potential of using these ligands as a novel antiviral strategy.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Vanessa Koo, Feng Tian, William W. L. Wong
Summary: HCV point-of-care testing is cost-effective for injection drug users, as it can prolong individuals' lifespan and save healthcare costs associated with HCV-related complications.
LIVER INTERNATIONAL
(2022)
Review
Biochemistry & Molecular Biology
Fan Yang, Zhigang Hu, Zhigang Guo
Summary: DNA damage repair is crucial for maintaining genomic stability and integrity, and FEN1 plays a critical role in this process. Abnormal expression or mutation of FEN1 is associated with various diseases, including cancers, making it a therapeutic target for cancer treatment. Small-molecule compounds targeting FEN1 have been developed and show promise in cancer therapy.
Article
Multidisciplinary Sciences
Payuda Hansoongnern, Pornpitra Pratedrat, Pornjarim Nilyanimit, Rujipat Wasitthankasem, Nawarat Posuwan, Nasamon Wanlapakorn, Kanchanok Kodchakorn, Prachya Kongtawelert, Napaporn Pimsing, Yong Poovorawan
Summary: Hepatitis C virus (HCV) is a viral pathogen that causes chronic hepatitis and can lead to cirrhosis and hepatocellular carcinoma. The detection of HCV RNA is the standard method for diagnosis and monitoring of antiviral treatment. A study investigated the correlation between HCV RNA and HCVcAg levels, as well as the impact of amino acid sequence heterogeneity on HCVcAg quantification. The findings showed a strong positive correlation between HCV RNA and HCVcAg, but samples with certain genotypes and amino acid substitutions exhibited lower HCVcAg levels than expected based on HCV RNA values.
Article
Chemistry, Multidisciplinary
Shuyu Jin, Shao-hua Zhuo, Yasushi Takemoto, Yan-mei Li, Motonari Uesugi
Summary: The effective co-delivery of antigens and immune potentiators, as well as high antigen presentation, are two major challenges in subunit vaccine development. In this study, peptide antigens were conjugated with cholicamide, a self-assembling small molecule adjuvant, to address these issues. Co-assemblies of the conjugates and cholicamide achieved high levels of both cytokine induction and MHC class II peptide presentation.
CHEMICAL COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Yue Luo, Junjun Cheng, Zhanying Hu, Haiqun Ban, Shuo Wu, Nicky Hwang, John Kulp, Yuhuan Li, Yanming Du, Jinhong Chang, Usha Viswanathan, Ju-Tao Guo
Summary: The study identified critical Cp amino acid residues for capsid assembly, pgRNA encapsidation, and resistance to CpAMs through systematic mutagenesis analysis. Specific residues such as W102 and Y132 are crucial for capsid assembly, while other residues affect pgRNA encapsidation. Several mutant Cp support high levels of DNA replication but show strong resistance to CpAMs.
ANTIVIRAL RESEARCH
(2021)
Review
Medicine, General & Internal
Jianjing Lin, Shicheng Jia, Weifei Zhang, Mengyuan Nian, Peng Liu, Li Yang, Jianwei Zuo, Wei Li, Hui Zeng, Xintao Zhang
Summary: Osteoarthritis is a degenerative disease characterized by joint pain, caused by factors such as fibrosis, chapping, ulcers, and loss of articular cartilage. Traditional treatments only delay its progression, while small molecule inhibitors targeting proteins show promising effects in managing osteoarthritis. This review examines small molecule inhibitors targeting various proteins and discusses disease-modifying osteoarthritis drugs based on them, providing valuable insights for the treatment of osteoarthritis.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Microbiology
Guillaume Mousseau, Cari F. Kessing, Remi Fromentin, Lydie Trautmann, Nicolas Chomont, Susana T. Valente
Article
Chemistry, Multidisciplinary
Hao Wu, Guillaume Mousseau, Sonia Mediouni, Susana T. Valente, Thomas Kodadek
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2016)
Article
Cell Biology
Cari F. Kessing, Christopher C. Nixon, Chuan Li, Perry Tsai, Hiroshi Takata, Guillaume Mousseau, Phong T. Ho, Jenna B. Honeycutt, Mohammad Fallahi, Lydie Trautmann, J. Victor Garcia, Susana T. Valente
Article
Biochemistry & Molecular Biology
Guillaume Mousseau, Quentin Raffy, Oliver P. Thomas, Morgane Agez, Robert Thai, Jean Philippe Renault, Serge Pin, Francoise Ochsenbein, Jean-Christophe Cintrat, Bernard Rousseau
Article
Microbiology
Guillaume Mousseau, Mark A. Clementz, Wendy N. Bakeman, Nisha Nagarsheth, Michael Cameron, Jun Shi, Phil Baran, Remi Fromentin, Nicolas Chomont, Susana T. Valente
CELL HOST & MICROBE
(2012)
Article
Biotechnology & Applied Microbiology
S. Kota, C. Coito, G. Mousseau, J. -P. Lavergne, A. D. Strosberg
JOURNAL OF GENERAL VIROLOGY
(2009)
Article
Genetics & Heredity
Chuan Li, Guillaume Mousseau, Susana T. Valente
EPIGENETICS & CHROMATIN
(2019)
Article
Chemistry, Organic
Sophie Oppilliart, Guillaume Mousseau, Li Zhang, Guochen Jia, Pierre Thuery, Bernard Rousseau, Jean-Christophe Cintrat
Article
Chemistry, Analytical
G. Mousseau, O. P. Thomas, S. Oppilliart, A. Coirier, A. Salcedo-Serna, R. Thai, F. Beau, J.-P. Renault, S. Pin, J.-C. Cintrat, B. Rousseau
ANALYTICAL CHEMISTRY
(2007)
Article
Biochemical Research Methods
V Sarramegna, I Muller, G Mousseau, C Froment, B Monsarrat, A Milon, F Talmont
PROTEIN EXPRESSION AND PURIFICATION
(2005)