Article
Immunology
Morgane Nennig, Ann-Katrin Llarena, Malte Herold, Joel Mossong, Christian Penny, Serge Losch, Odile Tresse, Catherine Ragimbeau
Summary: A study on Campylobacter jejuni in Luxembourg revealed an unexpected endemic pattern in the genetic profiles, and tested the hypothesis of stable lineages occurrence by implementing whole genome sequencing (WGS). Results showed that regardless of the typing scheme used, epidemic or endemic signals were detected in the lineages, supporting the clonal expansion of stable genomes in the Campylobacter population.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
Malgorzata Emanowicz, Joseph Meade, Catherine Burgess, Declan Bolton, John Egan, Helen Lynch, Lisa O'Connor, Aidan Coffey, Brigid Lucey, Montserrat Gutierrez, William Byrne, Rosemarie Slowey, Paul Whyte
Summary: Phenotypic antimicrobial resistance testing was conducted on 160 Campylobacter jejuni isolates from broiler samples to determine prevalence and distribution of resistance profiles. Whole genome sequencing of 132 isolates revealed a high correlation between resistant phenotypes and genotypes, with identified resistance determinants including tet(O) genes and gyrA p. T86I mutation. Multiple sequence types (ST) were identified, with ST-257 being prevalent, and virulence genes associated with human infection and broiler colonization were detected.
Article
Microbiology
Ying-Shu Liao, Bo-Han Chen, Ru-Hsiou Teng, You-Wun Wang, Jui-Hsien Chang, Shiu-Yun Liang, Chi-Sen Tsao, Yu-Ping Hong, Hui-Yung Sung, Chien-Shun Chiou
Summary: Research in Taiwan has shown that C. coli and C. jejuni are highly resistant to most therapeutic antimicrobials. Whole-genome sequencing was used to analyze genetic diversity among isolates and genetic resistance determinants associated with antimicrobial resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Food Science & Technology
Fuat Aydin, Tuba Kayman, Secil Abay, Harun Hizlisoy, Izzet Burcin Saticioglu, Emre Karakaya, Orhan Sahin
Summary: This study investigated the genetic diversity, prevalence, and mechanisms of resistance to quinolone antibiotics in 178 Campylobacter jejuni isolates from humans, cattle, dogs, and chickens in Turkey. The results showed that there were 21 clonal complexes (CCs) and 78 different sequence types (STs) among the isolates, with CC21, CC353, CC206, and CC257 being the predominant clones. Quinolone resistance was detected in 41% of the isolates, and all resistant isolates had a specific mutation in the gyrA gene. The study provided important insights into the transmission pathways of C. jejuni in Turkey, suggesting that broiler meat and dogs may be the most important sources of human campylobacteriosis in the country.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Lucia Rivas, Pierre-Yves Dupont, Brent Gilpin, Helen Withers
Summary: A pilot survey in New Zealand showed that the prevalence of Campylobacter in ovine carcass trim was 33% based on PCR detection, with hogget carcasses having the highest prevalence at 56%. Twenty-five MLST sequence types were identified among isolates, indicating genetic diversity among ovine isolates collected. Whole genome sequencing analysis discriminated isolates within a single sequence type.
JOURNAL OF FOOD PROTECTION
(2021)
Article
Biotechnology & Applied Microbiology
Deirdre M. Prendergast, Helen Lynch, Paul Whyte, Olwen Golden, Declan Murphy, Montserrat Gutierrez, Juliana Cummins, Dayle Johnston, Declan Bolton, Aidan Coffey, Brigid Lucey, Lisa O'Connor, William Byrne
Summary: This study used whole genome sequencing to analyze the genomic diversity of Campylobacter jejuni and identified virulence genes. The results suggest that caecal colonization is the main source of C. jejuni contamination in broiler carcasses, rather than cross-contamination from other batches or the environment.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Hosny El-Adawy, Helmut Hotzel, Silvia Garcia-Soto, Herbert Tomaso, Hafez M. Hafez, Stefan Schwarz, Heinrich Neubauer, Joerg Linde
Summary: This study used whole-genome sequencing to assess the epidemiology and genetic heterogeneity of C. jejuni in commercial turkey farms in Germany. The results showed high genetic diversity among the isolates, with various antimicrobial resistance genes and virulence-associated genes. The study highlights the potential of whole-genome sequencing for surveillance of C. jejuni and emphasizes the need for updates in resistance gene databases.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Microbiology
Jose F. Cobo-Diaz, Paloma Gonzalez del Rio, Avelino Alvarez-Ordonez
Summary: Campylobacter spp. is the most frequent agent of human gastroenteritis worldwide, and the current study presents the resistome analysis of different strains. Resistance to beta-lactams and tetracyclines are the most common, with quinolone resistance on C. jejuni and aminoglycoside resistance on C. coli also important. Geographic differences were found in clonal complexes distribution and resistome genotypes, with certain gene patterns associated with specific sources of isolation.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Xiang Wang, Qiyun Zhuo, Yi Hong, Yufan Wu, Qiang Gu, Dawei Yuan, Qingli Dong, Jingdong Shao
Summary: This study investigated the antibiotic resistance and virulence potential of 100 Campylobacter jejuni isolates from poultry meat. It found a correlation between the multilocus sequence types (MLST) of the isolates and their antibiotic resistance or virulence. The results provide critical information for the risk assessment of campylobacteriosis infection.
Article
Biotechnology & Applied Microbiology
I. Hansson, L. -M. Tamminen, S. Frosth, L. -L. Fernstrom, U. Emanuelson, S. Boqvist
Summary: This study investigated the occurrence of Campylobacter, species present, and antibiotic resistance in Swedish cattle. The findings revealed a strong association between sequence type and antimicrobial resistance pattern, with a high proportion of quinolone-resistant Campylobacter jejuni in calves, highlighting the importance of considering this in a One Health perspective.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Infectious Diseases
Nehal A. Saif, Jose F. Cobo-Diaz, Menattallah Elserafy, Iman El-Shiekh, Avelino Alvarez-Ordonez, Shaimaa F. Mouftah, Mohamed Elhadidy
Summary: This study characterized Campylobacter jejuni from different sources in Egypt using multiple typing techniques. The analysis revealed that cgMLST and host-segregating markers were the most discriminative methods, and identified retail chicken and cattle reservoir as important sources of C. jejuni infection in Egypt. The findings emphasize the importance of using whole genome sequencing typing methods for source tracking of C. jejuni and suggest the role of non-poultry sources in human campylobacteriosis control measures in Egypt.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Microbiology
Andrea Laconi, Ilenia Drigo, Nicola Palmieri, Lisa Carraro, Elena Tonon, Raffaela Franch, Luca Bano, Alessandra Piccirillo
Summary: The study revealed that Campylobacter isolates from chicken livers possessed a higher number of virulence-associated genes, which might contribute to the development of pathological lesions in these isolates within the host.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Daichi Morita, Hiroki Arai, Junko Isobe, Emi Maenishi, Takanori Kumagai, Fumito Maruyama, Teruo Kuroda
Summary: Campylobacter jejuni is a major cause of enteritis and diarrhea in humans. Drug-resistant Campylobacter is increasing globally, and fluoroquinolone-resistant Campylobacter is of particular concern. Surveillance of Campylobacter drug resistance is important to address this issue.
MICROBIOLOGY SPECTRUM
(2023)
Article
Genetics & Heredity
Anastasia-Lisa Dieckmann, Thomas Riedel, Boyke Bunk, Cathrin Sproeer, Joerg Overmann, Uwe Gross, Oliver Bader, Wolfgang Bohne, Burkhard Morgenstern, Morteza Hosseini, Andreas E. Zautner
Summary: The recent discovery of mixed Campylobacter coli strains suggests that C. coli and C. jejuni may be going through a process of accelerated gene transfer termed 'despeciation'. The phylogenetic tree revealed a separate cluster of Campylobacter isolates that possibly indicates genetic introgression. These hybrid strains, resulting from genetic introgression between C. coli and C. jejuni, have a genomic mosaic structure influenced by C. jejuni.
MICROBIAL GENOMICS
(2021)
Article
Microbiology
Hiroshi Asakura, Shiori Yamamoto, Kazuhiro Yamada, Jun Kawase, Hiromi Nakamura, Kou-ichiro Abe, Yoshimasa Sasaki, Tetsuya Ikeda, Ryohei Nomoto
Summary: Campylobacter jejuni and C. coli are major causes of gastrointestinal illnesses transmitted to humans mainly through chicken meats. This study examined the levels of bacterial contamination in retail chicken meats in Japan and found that 45.7% of the samples were positive, with 8.4% exceeding the safe limit. Bacterial counts were higher in fall compared to spring and summer, and younger slaughtered chickens were more contaminated. The study also identified antimicrobial resistance genes and clonal complexes of C. jejuni isolates.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Ecology
Bram Flahou, Mirko Rossi, Jaco Bakker, Jan A. M. Langermans, Edwin Heuvelman, Jay V. Solnick, Miriam E. Martin, Jani O'Rourke, Le Duc Ngoan, Nguyen Xuan Hoa, Masahiko Nakamura, Anders Overby, Hidenori Matsui, Hiroyoshi Ota, Takehisa Matsumoto, Dennis L. Foss, Laurice A. Kopta, Oladipo Omotosho, Maria Pia Franciosini, Patrizia Casagrande Proietti, Aizhen Guo, Han Liu, Gabriela Borilova, Ana Paula Bracarense, Sara K. Linden, Sofie De Bruyckere, Guangzhi Zhang, Chloe De Witte, Annemieke Smet, Frank Pasmans, Richard Ducatelle, Jukka Corander, Freddy Haesebrouck
Review
Infectious Diseases
J. A. Carrico, M. Rossi, J. Moran-Gilad, G. Van Domselaar, M. Ramirez
CLINICAL MICROBIOLOGY AND INFECTION
(2018)
Article
Ecology
Annemieke Smet, Koji Yahara, Mirko Rossi, Alfred Tay, Steffen Backert, Ensser Armin, James G. Fox, Bram Flahou, Richard Ducatelle, Freddy Haesebrouck, Jukka Corander
Article
Multidisciplinary Sciences
Alejandra Culebro, Miguel P. Machado, Joao Andre Carrico, Mirko Rossi
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Federica Palma, Gerardo Manfreda, Mickael Silva, Antonio Parisi, Dillon O. R. Barker, Eduardo N. Taboada, Frederique Pasquali, Mirko Rossi
SCIENTIFIC REPORTS
(2018)
Article
Biotechnology & Applied Microbiology
Sara Kovanen, Mirko Rossi, Mari Pohja-Mykra, Timo Nieminen, Mirja Raunio-Saarnisto, Mikaela Sauvala, Maria Fredriksson-Ahomaa, Marja-Liisa Hanninen, Rauni Kivisto
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Anniina Jaakkonen, Hanna Castro, Saija Hallanvuo, Jukka Ranta, Mirko Rossi, Joana Isidro, Miia Lindstrom, Marjaana Hakkinen
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Correction
Ecology
Annemieke Smet, Koji Yahara, Mirko Rossi, Alfred Tay, Steffen Backert, Armin Ensser, James G. Fox, Bram Flahou, Richard Ducatelle, Freddy Haesebrouck, Jukka Corander
Article
Microbiology
Alejandra Kolehmainen, Mirko Rossi, Jacek Stupak, Jianjun Li, Michel Gilbert, Warren Wakarchuk
JOURNAL OF BACTERIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Lourdes Garcia-Sanchez, Beatriz Melero, Isabel Jaime, Mirko Rossi, Irene Ortega, Jordi Rovira
Article
Microbiology
Ann-Katrin Llarena, Rauni Kivisto
Article
Microbiology
Satu Olkkola, Mirko Rossi, Anniina Jaakkonen, Maria Simola, Jouni Tikkanen, Marjaana Hakkinen, Pirkko Tuominen, Otso Huitu, Jukka Niemimaa, Heikki Henttonen, Rauni Kivisto
Summary: The study found that C. jejuni was the only Campylobacter species identified in small wild mammals in Finland and in samples collected near 40 farms, with the highest prevalence in yellow-necked mice and bank voles. Through whole-genome sequencing analysis, it was suggested that C. jejuni strains from voles may represent a new previously undescribed species. The research also showed that the phylogeny of the isolates followed host species-association, rather than origin from either farm or wild captured environments.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Brajabandhu Pradhan, Janine Liedtke, Mike Sleutel, Toril Lindback, Ephrem Debebe Zegeye, Kristin O'Sullivan, Ann-Katrin Llarena, Ola Brynildsrud, Marina Aspholm, Han Remaut
Summary: Research shows that the unique endospore appendages Enas of Bacillus cereus represent a novel class of Gram-positive pili, highly resistant to heat, drought, and chemical damage. Phylogenomic analysis reveals the ubiquitous presence of the ena-gene cluster in the B. cereus group, including species of clinical, environmental, and food importance. Enas are proposed to be a specialized type of pili adapted to the harsh conditions encountered by bacterial spores.
Article
Agriculture, Dairy & Animal Science
Kristin M. Herstad, Hilde Vinje, Ellen Skancke, Terese Naeverdal, Francisca Corral, Ann-Katrin Llarena, Romy M. Heilmann, Jan S. Suchodolski, Joerg M. Steiner, Nicole Frost Nyquist
Summary: This study aimed to investigate whether canine-obtained lactic acid bacteria affect the frequency of diarrhea, fecal microbiota, and gastrointestinal inflammation in dogs receiving NSAIDs. The results showed that LAB did not significantly reduce the occurrence of diarrhea, change fecal microbiota, or impact gastrointestinal inflammation in dogs treated with NSAIDs.
Article
Genetics & Heredity
Mickael Silva, Miguel P. Machado, Diogo N. Silva, Mirko Rossi, Jacob Moran-Gilad, Sergio Santos, Mario Ramirez, Joao Andre Carrico
MICROBIAL GENOMICS
(2018)