Article
Food Science & Technology
Rui Dong, Xiaojie Qin, Shoukui He, Xiujuan Zhou, Yan Cui, Chunlei Shi, Yiping He, Xianming Shi
Summary: The study revealed that DsrA plays a crucial role in promoting oxidative stress resistance in S. Typhimurium by regulating the expression of antioxidant enzymes and maintaining intracellular redox balance.
Article
Microbiology
Yu Han, Yu Li, Zhiwei Zeng, Wenjing Li, Saixiang Feng, Weisheng Cao
Summary: This study explores the resistance mechanism and activity of the antimicrobial peptide MccY against Salmonella strains. The deletion of Ton system genes resulted in resistance to MccY and mutants showed increased susceptibility to MccY under a low-iron condition. MccY may decrease the virulence of S. Typhimurium by altering multiple physiological properties.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biology
Sion C. Bayliss, Rebecca K. Locke, Claire Jenkins, Marie Anne Chattaway, Timothy J. Dallman, Lauren A. Cowley, Ben S. Cooper
Summary: This study developed a hierarchical machine learning model to rapidly identify and trace the geographical source of Salmonella enterica serovar Enteritidis infections from whole genome sequencing data. The model achieved high accuracy in predicting the continent, sub-region, and country of origin, and provides results within 4 minutes per sample, facilitating rapid outbreak resolution and real-time genomic epidemiology.
Article
Chemistry, Analytical
Han Du, Xu Zhang, Mingru Yao, Qingli Yang, Wei Wu
Summary: This study successfully constructed aptamer-guided luminous microspheres using carbon dots fluorescence, which can efficiently recognize and detect Salmonella enterica serovar Typhimurium. The method shows high sensitivity and has broad application prospects.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Microbiology
Stephanie Paulini, Florian D. Fabiani, Anna S. Weiss, Ana Laura Moldoveanu, Sophie Helaine, Baerbel Stecher, Kirsten Jung
Summary: Pyruvate, the simplest of the alpha-keto acids, plays a crucial role in the metabolic pathways of bacteria and eukaryotes. This study identified two specific transporters, BtsT and CstA, in Salmonella enterica serovar Typhimurium, which are important for pyruvate uptake and have significant effects on bacterial survival, infection, and persistence.
Article
Microbiology
Jiwon Baek, Hyunjin Yoon
Summary: Cyclic di-GMP is a signaling molecule that controls the transition between motile and nonmotile lifestyles in bacteria. It regulates biofilm formation and dispersal, and it can alter carbon metabolic pathways in Salmonella enterica serovar Typhimurium.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Li Long, Lv You, Dan Wang, Ming Wang, Junhua Wang, Guihuan Bai, Jianhua Li, Xiaoyu Wei, Shijun Li
Summary: This study investigated the antimicrobial resistance, presence of antimicrobial resistance genes and virulence genes, and MLST genotypes of Salmonella enterica serovar 4,[5],12:i:- isolates from Guizhou province, China. The results showed that the isolates displayed high resistance to multiple antibiotics and carried various antimicrobial resistance genes and virulence genes. The study provided important information on Salmonella enterica serovar 4,[5],12:i:- infections in humans from Guizhou province.
Article
Microbiology
R. Kavi Bharathi, C. S. Srinandan, N. Sai Subramanian
Summary: This study investigates the cooperative ability and matrix sharing between specialist and generalist strains during biofilm formation. The results show that the matrix aids in survival during antibiotic, chlorine, and predatory stress, and possible sharing of the matrix occurs in co-culture.
Article
Multidisciplinary Sciences
Danielle J. Ingle, Rebecca L. Ambrose, Sarah L. Baines, Sebastian Duchene, Anders Goncalves da Silva, Darren Y. J. Lee, Miriam Jones, Mary Valcanis, George Taiaroa, Susan A. Ballard, Martyn D. Kirk, Benjamin P. Howden, Jaclyn S. Pearson, Deborah A. Williamson
Summary: Three distinct lineages of Salmonella 4,[5],12:i:- have emerged in Australia, likely reflecting differential antimicrobial selection pressures, with two lineages associated with travel to South-East Asia and the third lineage endemic to Australia. These antimicrobial-resistant lineages efficiently infect and survive in host cells, suggesting a suppression of host immune response facilitating their persistence.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Seul Kim, Eunsuk Kim, Hyunjin Yoon
Summary: Salmonella alters cellular processes to improve intracellular fitness during host infection by rewiring cellular transcriptional regulation through alternative sigma factors such as rpoS. Deprivation of outer membrane protein YcfR in Salmonella Typhimurium leads to decreased intracellular survival and downregulation of SPI-2 genes, which is abolished in the absence of rpoS. RpoS-associated stress responses in Salmonella due to impaired envelope integrity may reciprocally downregulate the expression of SPI-2 genes to reduce its virulence.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Yuan Yuan, Yara Seif, Kevin Rychel, Reo Yoo, Siddharth Chauhan, Saugat Poudel, Tahani Al-bulushi, Bernhard O. Palsson, Anand Sastry
Summary: By combining pan-genome and transcriptomic analytics, variations in transcriptional regulatory networks across six strains of Salmonella enterica serovar Typhimurium were revealed.
Article
Food Science & Technology
Hui Li, Keren Agyekumwaa Addo, Yi-gang Yu, Xing-long Xiao
Summary: The study investigated the influence of cuminaldehyde (CUM) on cell membrane and genomic DNA of Salmonella enterica serovar Typhimurium (S. Typhimurium), and revealed its antibacterial mechanism. CUM could bind to bacterial membrane proteins and change its structure and conformation, leading to the leakage of intracellular substances and the destruction of cell integrity. DNA interaction analysis showed that CUM bound to genomic DNA through groove mode, affecting the normal functioning of S. Typhimurium.
Article
Nutrition & Dietetics
Weihua Tan, Yuanyuan Tian, Qingya Zhang, Siwei Miao, Wenrong Wu, Xiaoqing Miao, Haiou Kuang, Wenchao Yang
Summary: In this study, it was found that Apis laboriosa honey (ALH) harvested in China has antibacterial activity against Salmonella enterica serovar Typhimurium (S. Typhimurium). The physicochemical parameters and antioxidant activity of ALH were influenced by the time and region of harvest. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ALH against S. Typhimurium were similar to UMF5+ manuka honey. Proteomic experiment revealed the antibacterial mechanism of ALH.
FRONTIERS IN NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Mingzhe Yang, Qingli Dong, Hongmei Niu, Jiaming Li, Zijie Lin, Muhammad Zohaib Aslam, Xiang Wang, Zhuosi Li, Yangtai Liu, Yue Ma, Xiaojie Qin
Summary: This study aims to investigate whether continuous exposure to sublethal concentrations of benzalkonium chloride (BC) could lead to acquired resistance of Salmonella enterica serovar 1,4,[5],12:i:- (S. 1,4,[5],12:i:-) to BC and other environmental stresses. The results showed that all tested strains exhibited increased BC tolerance and antibiotic resistance after exposure to gradually increasing concentrations of BC. In addition, BC-adapted strains showed reduced zeta potential and increased resistance to heat and acid.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Food Science & Technology
Ji-Yeon Kim, Hana Song, Danbi Kim, Sun-Young Lee
Summary: Mild heat treatment can enhance the thermal resistance of S. Typhimurium and reduce membrane damage and heat-induced injury, leading to increased tolerance against heat, alcohol, and salt stress. Further research into the heat shock response mechanism of S. Typhimurium may provide insights for developing microbial control measures in food processing.
Article
Infectious Diseases
Gianvito Lanave, Daniela Loconsole, Francesca Centrone, Cristiana Catella, Paolo Capozza, Georgia Diakoudi, Antonio Parisi, Elisabetta Suffredini, Alessio Buonavoglia, Michele Camero, Maria Chironna, Vito Martella
Summary: A VA1-like AstV was identified from an outbreak of foodborne acute gastroenteritis in Italy in 2018, with genome sequencing showing a relation to the UK1 strain. Similar viruses were also found in pediatric patients with acute gastroenteritis in 2018, although at a low prevalence. Gathering epidemiological data on atypical AstVs is important for assessing the risks posed by such infections in medically fragile patients.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Federica Palma, Nicolas Radomski, Alizee Guerin, Yann Sevellec, Benjamin Felix, Arnaud Bridier, Christophe Soumet, Sophie Roussel, Laurent Guillier
Summary: The study found that mobile elements were associated with biocide tolerance in Lm strains. These elements, including prophage-related loci, the Tn6188_qacH transposon, and pLMST6_emrC plasmid, were widespread in food strains and linked to tolerance to benzalkonium-chloride and didecyldimethylammonium-chloride. Additionally, genes encoding for cell-surface proteins were associated with tolerance to benzalkonium-chloride and polymeric biguanide.
Article
Agriculture, Dairy & Animal Science
Lucia Gambi, Cecilia Crippa, Alex Lucchi, Alessandra De Cesare, Antonio Parisi, Gerardo Manfreda, Frederique Pasquali
Summary: Several strategies have been implemented to reduce unnecessary use of antimicrobials in food animal production, with a focus on evaluating their impact on antimicrobial resistance (AMR) genes. A study in Italy analyzed the occurrence of AMR genes in commensal E. coli from antibiotic-free farms, revealing lower occurrence rates compared to public genomes. Further research is needed to confirm these preliminary findings and assess the impact of prudent antimicrobial use on AMR in broiler production.
Article
Food Science & Technology
Alessandra De Cesare, Chiara Oliveri, Alex Lucchi, Federica Savini, Gerardo Manfreda, Claudia Sala
Summary: This study investigated whether raising chickens without antibiotics would affect the microbiome of poultry meat. The results showed that antibiotic-free farming resulted in significantly lower antimicrobial resistance load in the meat, although this difference was lost on the carcasses.
Article
Biotechnology & Applied Microbiology
Federica Palma, Iolanda Mangone, Anna Janowicz, Alexandra Moura, Alexandra Chiaverini, Marina Torresi, Giuliano Garofolo, Alexis Criscuolo, Sylvain Brisse, Adriano Di Pasquale, Cesare Camma, Nicolas Radomski
Summary: This study identified parameters influencing the precision of cgMLST profiles in Listeria monocytogenes, highlighting the impact of genetic background, cgMLST workflows, completeness, depth, and breadth of coverage on precision. All tested workflows performed well at >= 40X depth of coverage, showing consistent cluster definitions using a reference cut-off of <= 7 allele differences. The research suggests that bioinformatics workflows dedicated to cgMLST allele calling are robust when paired-end reads are of high quality and sequencing depth is sufficient.
Article
Virology
Luigia Trabace, Lorenzo Pace, Maria Grazia Morgese, Isabel Bianca Santo, Domenico Galante, Stefania Schiavone, Dora Cipolletta, Anna Maria Rosa, Pierluigi Reveglia, Antonio Parisi, Paolo Tucci, Giovanni Pepe, Rodolfo Sacco, Maria Pia Foschino Barbaro, Gaetano Corso, Antonio Fasanella
Summary: The Delta variant has raised concerns about its ability to evade SARS-CoV-2 vaccines. This study evaluated the neutralizing response of 172 Italian healthcare workers three months after receiving the Comirnaty vaccine. The results showed that the vaccine provides sustained neutralizing antibody activity against the Alpha variant, but it is less effective against the Gamma and even less against the Delta variants.
Article
Multidisciplinary Sciences
Giorgio Brugaletta, Alessandra De Cesare, Luca Laghi, Gerardo Manfreda, Marco Zampiga, Chiara Oliveri, Estefania Perez-Calvo, Gilberto Litta, Susanna Lolli, Federico Sirri
Summary: This study investigates the mechanisms of action of a novel dietary muramidase on broiler chickens using multi-omics analysis. The results show that the addition of muramidase significantly improves feed intake, body weight, and feed conversion ratio in broilers. It also affects the gut microbiota structure and metabolic profile at the caecal and blood levels.
SCIENTIFIC REPORTS
(2022)
Article
Infectious Diseases
Silvia Bonardi, Clotilde Silvia Cabassi, Gerardo Manfreda, Antonio Parisi, Enrico Fiaccadori, Alice Sabatino, Sandro Cavirani, Cristina Bacci, Martina Rega, Costanza Spadini, Mattia Iannarelli, Cecilia Crippa, Ferdinando Ruocco, Frederique Pasquali
Summary: This study investigates the resistance to carbapenems and third-generation cephalosporins in Gram-negative microorganisms isolated from swine, and evaluates the transmission of these resistant bacteria between pigs and humans. The results suggest the possible circulation of resistant bacteria between pigs and patients, indicating a potential public health concern.
Article
Environmental Sciences
Daniela Loconsole, Anna Sallustio, Francesca Centrone, Daniele Casulli, Marisa Accogli, Annalisa Saracino, Caterina Foti, Mauro Grandolfo, Giovanni Battista Buccoliero, Viviana Vitale, Sara De Nitto, Michele Conversano, Francesco Desiante, Laura Del Sambro, Domenico Simone, Antonio Parisi, Rosa Prato, Domenico Martinelli, Maria Chironna
Summary: This study describes the characteristics of MPXV infection identified in Southern Italy. The research found 10 cases, mostly young adult males including MSMs and females. The study also identified infections in females without sexual risk factors.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Frederique Pasquali, Antonio Valero, Aricia Possas, Alex Lucchi, Cecilia Crippa, Lucia Gambi, Gerardo Manfreda, Alessandra De Cesare
Summary: A longitudinal survey was conducted in an Italian artisanal cheese factory, revealing inter- and intra-batch variability in physicochemical and microbial parameters of the cheese. Higher levels of microbial contamination were associated with drains in the warm room and the packaging area. No verotoxigenic Escherichia coli or Listeria monocytogenes were found, but some Staphylococcus aureus and Salmonella pullorum isolates were identified in the cheese samples. Further investigation is needed to understand the potential pathogenicity and antimicrobial resistance of the identified Enterobacteriaceae in artisanal cheeses.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Frederique Pasquali, Lucia Gambi, Alessandra De Cesare, Cecilia Crippa, Vasco Cadavez, Ursula Gonzales-Barron, Antonio Valero, Fouad Achemchem, Alex Lucchi, Antonio Parisi, Gerardo Manfreda
Summary: This study investigated the diversity of resistome and virulome in artisanal fermented meat and dairy products and their production environments in Portugal, Spain, Italy, and Morocco. The results showed the circulation of different clones of Listeria monocytogenes, Salmonella enterica, and S. aureus in the same production plant, as well as the presence of virulent and hypervirulent strains. This highlights the need for specific prevention and control measures to reduce the risk of these biological hazards in artisanal food production.
ITALIAN JOURNAL OF FOOD SAFETY
(2022)
Article
Food Science & Technology
Lucia Gambi, Cecilia Crippa, Alex Lucchi, Gerardo Manfreda, Alessandra de Cesare, Frederique Pasquali
Summary: The occurrence of various bacteria in artisanal Italian salami was investigated in this study, with emphasis on the changes during the ripening period. The results showed an overall increase in total bacterial count and lactic acid bacteria, as well as a decrease in Enterobacteriaceae, pH, and water activity. No significant difference was observed between winter and summer batches. Potential foodborne pathogens like E. coli VTEC, Salmonella spp., and L. monocytogenes were not found, but Klebsiella pneumoniae was detected in both batches. The ripening period effectively controlled the occurrence of microbiological hazards in the finished salami, except for one isolate. Further studies are needed to assess the virulence potential of Klebsiella pneumoniae isolates.
ITALIAN JOURNAL OF FOOD SAFETY
(2023)
Article
Multidisciplinary Sciences
Cecilia Crippa, Frederique Pasquali, Carla Rodrigues, Alessandra De Cesare, Alex Lucchi, Lucia Gambi, Gerardo Manfreda, Sylvain Brisse, Federica Palma
Summary: This study reports and characterizes Klebsiella spp. isolates from two artisanal ready-to-eat food producing facilities and tracks similar genotypes in different ecological niches. Despite high genetic diversity, clonal strains of Klebsiella were found to persist in the same processing setting for over 14 months. Different genotypes from distinct sources in the same facility shared a plasmid, indicating the importance of surveillance in the food chain for understanding the circulation of potentially pathogenic strains.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Frederique Pasquali, Cecilia Crippa, Antonio Parisi, Alex Lucchi, Lucia Gambi, Alessandra Merlotti, Daniel Remondini, Maurizio Stonfer, Gerardo Manfreda
Summary: The aim of this study was to investigate the genetic diversity and antimicrobial resistance of E. coli during enrofloxacin therapy in broilers with colisepticemia. The results showed an increased population diversity after treatment, with an increase in fluoroquinolone resistance in E. coli isolates. Qnr-carrying plasmids did not persist, but two persistent conjugative AMR plasmid clusters were identified. Further studies are needed to confirm plasmid persistence not associated with antimicrobial selective pressure.