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Biochemistry & Molecular Biology
N. P. Syamprasad, Siddhi Jain, Bishal Rajdev, Samir Ranjan Panda, Jagadeesh Kumar Gangasani, Veerabhadra Swamy Challa, Jayathirtha Rao Vaidya, Gopal C. Kundu, V. G. M. Naidu
Summary: The cardioprotective effect of AKR1B1 inhibitor NARI-29 was evaluated in this study. The results showed that NARI-29 increased the sensitivity of breast cancer cells to Dox, while reducing its toxicity to cardiomyocytes and decreasing the secretion of inflammatory cytokines. Animal experiments also confirmed the protective effect of NARI-29 on Dox-induced cardiac injury.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Westermann, Amma G. Adomako-Bonsu, Solveig Thiele, Serhat Sezai Cicek, Hans-Joerg Martin, Edmund Maser
Summary: Members of the aldo-keto reductase and short-chain dehydrogenase/reductase enzyme superfamilies catalyze the conversion of a wide range of substrates, and inhibitors of such enzymes may improve therapeutic outcomes. Plant-derived anthraquinones have been used for medicinal purposes and in this study, selected anthrone and anthraquinone derivatives were tested on six recombinant human carbonyl reducing enzymes, showing potential for future drug development.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Review
Biochemical Research Methods
Siva Nageswara Rao Gajula, Megha Sajakumar Pillai, Gananadhamu Samanthula, Rajesh Sonti
Summary: The assessment of drug candidate's potential to inhibit cytochrome P450 (CYP) enzymes is crucial in pharmaceutical drug discovery and development. Pharmaceutical companies are diligent in limiting CYP inhibition liabilities of drug candidates in the early stages to avoid increased toxicity from drug-drug interactions (DDIs), and conducting risk assessments throughout the drug development process.
Article
Nanoscience & Nanotechnology
Yuki Koba, Masahiko Nakamoto, Michiya Matsusaki
Summary: This article introduces a polymer-based inhibitor that can simultaneously target two enzymes on the surface of cancer cells, effectively inhibiting the growth and migration of breast cancer cells. The hetero-multivalent interactions between the polymer and enzymes on the cancer cell membrane lead to synergistic inhibition of cancer cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Pharmacology & Pharmacy
Tatsuki Fukami, Tsuyoshi Yokoi, Miki Nakajima
Summary: This review provides an overview of the metabolism of clinically used drugs and highlights the roles of cytochrome P450 enzymes and non-P450 phase I enzymes in drug metabolism. It emphasizes the importance of studying non-P450 phase I enzymes for improving drug efficacy and reducing adverse reactions.
ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nour Elaimane Bouzidi, Samir Borhane Grama, Aboubakeur Essedik Khelef, Duanpeng Yang, Jian Li
Summary: This study investigates the effect of inhibiting antioxidant enzyme activity on carotenoid synthesis in microalgae. The findings suggest that inhibiting enzyme activity can significantly increase carotenoid accumulation, providing a new approach for carotenoid production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michela Cantiello, Monica Carletti, Mery Giantin, Giulia Gardini, Francesca Capolongo, Paolo Cascio, Marianna Pauletto, Flavia Girolami, Mauro Dacasto, Carlo Nebbia
Summary: This study provides a comprehensive characterization of phenobarbital's (PB) effects on drug-metabolizing enzyme (DME) catalytic activities in cattle liver. The findings show that PB increases the protein amounts and enzyme activities of certain target enzymes, but has contradictory effects on others. The study also reveals an increase in 26S proteasome activity, suggesting a potential role in cattle DME regulation. The results further confirm species differences in DME expression and activity between cattle, humans, and rodents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Feng Zhang, Wei Liu, Jian Huang, Qi-long Chen, Dan-dan Wang, Li-wei Zou, Yong-fang Zhao, Wei-dong Zhang, Jian-guang Xu, Hong-zhuan Chen, Guang-bo Ge
Summary: Jingyin granules, an antiviral herbal medicine, are commonly used to treat viral infections. Research shows that the herbal extract of Jingyin granules can inhibit human drug-metabolizing enzymes and modulate the plasma exposure and half-life of certain drugs.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Food Science & Technology
Feng Zhang, Jian Huang, Wei Liu, Chao-Ran Wang, Yan-Fang Liu, Dong-Zhu Tu, Xin-Miao Liang, Ling Yang, Wei-Dong Zhang, Hong-Zhuan Chen, Guang-Bo Ge
Summary: The study found that Qingfei Paidu decoction can inhibit multiple drug-metabolizing enzymes, with a significant inhibitory effect on CYP3A. In vivo experiments showed that co-administration of QPD with lopinavir could prolong the half-life of lopinavir and increase its AUC, and further investigations revealed that Fructus Aurantii Immaturus in QPD may cause significant loss of CYP3A activity.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Environmental Sciences
Marwa H. H. El-Sayed, Mohamed M. A. Ibrahim, Ahmed E. A. Elsobki, Ahmed A. A. Aioub
Summary: The use of insecticides in agriculture can lead to insect resistance. This study examined the effects of three enzyme inhibitors on the toxicity of two insecticides in Spodoptera littoralis larvae. The results showed that the enzyme inhibitors increased the toxicity of the insecticides.
Article
Biochemistry & Molecular Biology
Marek Drozdzik, Joanna Lapczuk-Romanska, Christoph Wenzel, Lukasz Skalski, Sylwia Szelag-Pieniek, Mariola Post, Arkadiusz Parus, Marta Syczewska, Mateusz Kurzawski, Stefan Oswald
Summary: Hepatic drug metabolizing enzymes (DMEs) play a crucial role in drug pharmacokinetics and can be affected by liver diseases. This study analyzed hepatitis C liver samples of different functional states and found that the protein abundances and mRNA levels of specific CYP and UGT enzymes were altered. The severity of the disease was associated with significant up-regulation or down-regulation of certain enzymes, indicating that hepatitis C virus infection impacts DMEs protein abundances in the liver.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Organic
Xinlong Zong, Shuanglei Liu, Zhenguo Zhang, Liang Ji, Ting Zhang, Zhenhua Jia, Teck-Peng Loh
Summary: The Mukaiyama-aldol reaction is an efficient strategy for synthesizing beta hydroxy carbonyl compounds. This study presents a protocol for a fluoride anion-mediated Mukaiyama aldol reaction, which incorporates fluorine at the alpha position into beta-hydroxy esters in a short reaction time. The method shows good functional-group tolerance and potential for scalability.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Review
Food Science & Technology
Heba Bedair, Hadeer Abdulrahman Rady, Aya Misbah Hussien, Meena Pandey, Wilgince Apollon, Samar Sami AlKafaas, Soumya Ghosh
Summary: This article discusses the serious environmental problem of food contamination and introduces the use of biosensors for detecting pesticides. The article also summarizes previous research on enzyme inhibition biosensors for pesticide detection and highlights different biological sources of these enzymes.
FOOD ANALYTICAL METHODS
(2022)
Article
Pharmacology & Pharmacy
Marek Drozdzik, Joanna Lapczuk-Romanska, Christoph Wenzel, Sylwia Szelag-Pieniek, Mariola Post, Lukasz Skalski, Mateusz Kurzawski, Stefan Oswald
Summary: The study found that the protein abundance of hepatic drug metabolizing enzymes is affected by both the type of liver pathology and the functional state of the organ. CYP2E1 is the most vulnerable enzyme, while the protein levels of CYP1A1, CYP2B6, CYP2C19, CYP2D6 as well as UGT1A1, UGT1A3 and UGT2B15 remain relatively stable during liver function deterioration.
Article
Biochemistry & Molecular Biology
Joanna Wawszczyk, Katarzyna Jesse, Malgorzata Kapral
Summary: This study aimed to evaluate the influence of pterostilbene on the proliferation and apoptosis of melanoma cells. The results revealed that pterostilbene reduced the proliferation of melanoma cells and induced apoptosis in a portion of them, while having no effect on another type of melanoma cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Lucie Raisova Stuchlikova, Vera Kralova, Katerina Lnenickova, Tomas Zarybnicky, Petra Matouskova, Veronika Hanusova, Martin Ambroz, Zdenek Subrt, Lenka Skalova
DRUG TESTING AND ANALYSIS
(2018)
Article
Environmental Sciences
Lucie Raisova Stuchlikova, Lenka Skalova, Barbora Szotakova, Eliska Syslova, Ivan Vokral, Tomas Vanek, Radka Podlipna
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2018)
Article
Oncology
Veronika Skarkova, Vera Kralova, Lukas Krbal, Petra Matouskov, Jiri Soukup, Emil Rudolf
EXPERIMENTAL CELL RESEARCH
(2018)
Article
Oncology
Ladislava Schroterova, Alena Jezkova, Emil Rudolf, Katerina Caltova, Vera Kralova, Veronika Hanusova
INTERNATIONAL JOURNAL OF ONCOLOGY
(2018)
Article
Parasitology
Lucie Raisova Stuchlikova, Petra Matouskova, Ivan Vokral, Jiri Lamka, Barbora Szotakova, Anna Seckarova, Diana Dimunova, Linh Thuy Nguyen, Marian Varady, Lenka Skalova
INTERNATIONAL JOURNAL FOR PARASITOLOGY-DRUGS AND DRUG RESISTANCE
(2018)
Article
Biochemistry & Molecular Biology
Vera Kralova, Veronika Hanusova, Katerina Caltova, Petr Spacek, Martina Hochmalova, Lenka Skalova, Emil Rudolf
CHEMICO-BIOLOGICAL INTERACTIONS
(2018)
Article
Oncology
Veronika Hanusova, Lenka Skalova, Vera Kralova, Petra Matouskova
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2018)
Review
Cell Biology
Veronika Skarkova, Vera Kralova, Barbora Vitovcova, Emil Rudolf
Article
Pharmacology & Pharmacy
Vladimir Kubicek, Lenka Skalova, Adam Skarka, Vera Kralova, Jana Holubova, Jana Stepankova, Zdenek Subrt, Barbora Szotakova
FRONTIERS IN PHARMACOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Veronika Skarkova, Marketa Krupova, Barbora Vitovcova, Adam Skarka, Petra Kasparova, Petr Krupa, Vera Kralova, Emil Rudolf
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Pharmacology & Pharmacy
Martin Ambroz, Marketa Smatova, Michaela Sadibolova, Eva Pospisilova, Pavlina Hadravska, Michaela Kasparova, Veronika Hanusova Skarkova, Vera Kralova, Lenka Skalova
ACTA PHARMACEUTICA
(2019)
Article
Pharmacology & Pharmacy
Katerina Lnenickova, Lenka Skalova, Lucie Stuchlikova Raisova, Barbora Szotakova, Petra Matouskova
ACTA PHARMACEUTICA
(2018)
Review
Toxicology
Tomas Zarybnicky, Iva Bousova, Martin Ambroz, Lenka Skalova
ARCHIVES OF TOXICOLOGY
(2018)