Article
Multidisciplinary Sciences
Aude Angelini, JoAnn Trial, Alexander B. Saltzman, Anna Malovannaya, Katarzyna A. Cieslik
Summary: The study focuses on the impaired myofibroblast development in aging male heart due to stiffer extracellular matrix (ECM), and shows decreased actin polymerization, α-smooth muscle actin (α-SMA), and Kindlin-2 expressions in old male cardiac fibroblasts. Manipulation of Kindlin-2 levels reversed the defective phenotype, and inhibition of overactivated ERK1/2 rescued actin polymerization and α-SMA expression. However, old female cardiac fibroblasts retained normal mechanosensing pathway.
Article
Biochemistry & Molecular Biology
Basalova Nataliya, Arbatskiy Mikhail, Popov Vladimir, Grigorieva Olga, Vigovskiy Maksim, Zaytsev Ivan, Novoseletskaya Ekaterina, Sagaradze Georgy, Danilova Natalia, Malkov Pavel, Cherniaev Andrey, Samsonova Maria, Karagyaur Maxim, Tolstoluzhinskaya Anastasiya, Dyachkova Uliana, Akopyan Zhanna, Tkachuk Vsevolod, Kalinina Natalia, Efimenko Anastasiya
Summary: Membrane-bound vesicles released by fat-derived stem cells contain small RNA molecules that help resolve lung scarring. These molecules cause scar-forming cells to become more flexible, leading to healthier lung function. Drugs targeting these molecules could potentially treat fibrosis in various organs.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Xue Liu, Simon C. Rowan, Jiurong Liang, Changfu Yao, Guanling Huang, Nan Deng, Ting Xie, Di Wu, Yizhou Wang, Ankita Burman, Tanyalak Parimon, Zea Borok, Peter Chen, William C. Parks, Cory M. Hogaboam, S. Samuel Weigt, John Belperio, Barry R. Stripp, Paul W. Noble, Dianhua Jiang
Summary: Pulmonary mesenchymal cells play critical roles in lung development and disease states in both mice and humans, showing high heterogeneity and subtype-specific transcriptomic differences which contribute to matrix gene expression in fibrosis. Comparative analysis reveals conserved transcriptomic signatures in orthologous subpopulations of murine and human lung mesenchymal cells, enhancing our understanding of mesenchymal cell diversity in lung development and disease conditions.
Article
Cell Biology
Rachel E. Bell, Tanya J. Shaw
Summary: Research suggests that myofibroblasts are not consistently present in primary keloid lesions, and keloid scars may not necessarily require alpha SMA-positive myofibroblasts. Viewing keloid scars on a quantitative spectrum with normal or hypertrophic scars, using alpha SMA as a biomarker of disease severity, is hindering progress in understanding the disease mechanisms and developing therapies.
WOUND REPAIR AND REGENERATION
(2021)
Article
Multidisciplinary Sciences
Ramasatyaveni Geesala, You-Min Lin, Ke Zhang, Suimin Qiu, Xuan-Zheng Shi
Summary: Inflammatory bowel diseases (IBD), such as Crohn's disease (CD), are chronic inflammatory disorders of the gastrointestinal tract. The pathogenesis of CD is not well understood, and current studies mainly focus on dysregulated gut immune response mechanisms. Mechanical stress may play an independent role in CD, but its exact impact is not defined. A TNBS-induced CD-like colitis model in rodents has been developed to study the pathogenic mechanisms, particularly the role of mechanical stress and mechanical stress-induced gene expression in immune dysregulation, intestinal fibrosis, and tissue remodeling in CD.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Multidisciplinary Sciences
Md Soriful Islam, Maclaine Parish, Joshua T. Brennan, Briana L. Winer, James H. Segars
Summary: Fibrosis in uterine fibroids can be restricted by inhibiting fibrotic processes, and Epigallocatechin gallate (EGCG) has shown promise as a drug for uterine fibroids. This study investigates the effects and mechanisms of EGCG on fibroid cell fibrosis and finds that EGCG reduces the expression of fibrotic proteins and regulates key signaling pathways. Furthermore, EGCG exhibits greater anti-fibrotic effects compared to synthetic inhibitors.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Fatmah Al-Habeeb, Noof Aloufi, Hussein Traboulsi, Xingxing Liu, Parameswaran Nair, Christina Haston, Ilan Azuelos, Steven K. Huang, Eric S. White, Imed E. Gallouzi, Sergio Di Marco, David H. Eidelman, Carolyn J. Baglole
Summary: The study revealed the significant role of HuR in promoting ECM production, myofibroblast differentiation, and lung fibrosis. Understanding the function of HuR could potentially lead to the development of new targeted therapy for fibrotic diseases such as Idiopathic Pulmonary Fibrosis (IPF).
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Thomas B. Layton, Lynn Williams, Jagdeep Nanchahal
Summary: We discuss the biology of Dupuytren's disease (DD), a common fibrotic disorder of the hand. The disease develops through the interaction of genetic and environmental factors, as well as epigenetic signaling. The cellular and molecular pathways in DD can be easily studied due to the accessibility of tissue in affected patients, and this research may have implications for understanding fibrotic tissues in other parts of the body.
TRENDS IN MOLECULAR MEDICINE
(2023)
Article
Multidisciplinary Sciences
Thanh-Diep Ly, Meike Sambale, Lara Kloesener, Philipp Traut, Bastian Fischer, Doris Hendig, Joachim R. Kuhn, Cornelius Knabbe, Isabel Faust-Hinse
Summary: Arthrofibrosis following total knee arthroplasty is characterized by dysregulated extracellular matrix protein synthesis and myofibroblast activation. This study investigated primary synovial fibroblasts from arthrofibrotic tissues and demonstrated increased cell contractility, XT secretion rate, and fibroblast-to-myofibroblast transition rate. The fibrotic phenotype in arthrofibrotic fibroblasts was confirmed by enhanced collagen and proteoglycan expression. These findings provide insights into the pathogenesis of arthrofibrosis and potential therapeutic targets.
Article
Biology
Sunisa Yoodee, Chadanat Noonin, Kanyarat Sueksakit, Rattiyaporn Kanlaya, Sakdithep Chaiyarit, Paleerath Peerapen, Visith Thongboonkerd
Summary: Using label-free quantitative proteomics, this study identified significantly altered secreted proteins from COM-exposed macrophages, suggesting their potential role in renal fibrogenesis in kidney stone disease.
COMMUNICATIONS BIOLOGY
(2021)
Article
Parasitology
Deping Cao, Emad Shamsan, Bofan Jiang, Haining Fan, Yaogang Zhang, Mustafa Abdo Saif Dehwah
Summary: The study found that cocultures of Echinococcus multilocularis protoscoleces and human hepatic stellate cells resulted in morphological changes and increased expression levels of hepatic fibrosis-related proteins. These findings provide insights into the molecular mechanisms of hepatic fibrosis induced by AE infection.
PARASITES & VECTORS
(2021)
Article
Pharmacology & Pharmacy
Mark W. Majesky, Mary C. M. Weiser -Evans
Summary: The adventitia is a complex and dynamic tissue that receives input signals from various sources to regulate the structural and mechanical properties of blood vessels. It plays a crucial role in vascular development, homeostasis, and repair.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Christian Baumeier, Felicitas Escher, Ganna Aleshcheva, Heiko Pietsch, Heinz-Peter Schultheiss
Summary: The study showed that elevated levels of PAI-1 in high-grade DCMi patients are associated with reduced cardiac fibrosis, decreased TGF-beta levels, and fewer myofibroblasts. Additionally, high-grade DCMi patients exhibited a higher proportion of M2 macrophages compared to M1 macrophages, suggesting a role of PAI-1 in macrophage polarization in inflammatory cardiomyopathies.
BASIC RESEARCH IN CARDIOLOGY
(2021)
Article
Medicine, General & Internal
Nataly Puerta Cavanzo, Emilia Bigaeva, Miriam Boersema, Peter Olinga, Ruud A. Bank
Summary: The optimal crowder for screening anti-fibrotic drugs is PVP40, based on comparisons with other macromolecular crowders. Various concentrations of Imatinib, Galunisertib, Omipalisib, and Nintedanib were tested in the presence of PVP40 to assess their effects on collagen deposition and myofibroblast formation.
FRONTIERS IN MEDICINE
(2021)
Article
Medicine, General & Internal
Marta S. Alexdottir, Arno R. Bourgonje, Morten A. Karsdal, Martin Pehrsson, Roberta Loveikyte, Hendrik M. van Dullemen, Marijn C. Visschedijk, Eleonora A. M. Festen, Rinse K. Weersma, Klaas Nico Faber, Gerard Dijkstra, Joachim H. Mortensen
Summary: This study aimed to assess the role of serological biomarkers in monitoring and predicting treatment response in patients with Crohn's disease undergoing infliximab induction therapy. The results showed that baseline levels of a biomarker for type IV collagen degradation were associated with treatment response, while biomarkers for type III and VI collagen formation could be used to monitor response at the end of treatment. Additionally, biomarker ratios for type IV collagen turnover showed promising results in monitoring treatment response in patients without a surgical history.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, Research & Experimental
Shuhei Fukunaga, Kotaro Kuwaki, Keiichi Mitsuyama, Hidetoshi Takedatsu, Shinichiro Yoshioka, Hiroshi Yamasaki, Ryosuke Yamauchi, Atsushi Mori, Tatsuyuki Kakuma, Osamu Tsuruta, Takuji Torimura
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2018)
Article
Gastroenterology & Hepatology
Hidetoshi Takedatsu, Keiichi Mitsuyama, Shuhei Fukunaga, Shinichiro Yoshioka, Ryosuke Yamauchi, Atsushi Mori, Hiroshi Yamasaki, Kotaro Kuwaki, Hideto Sakisaka, Shotaro Sakisaka, Takuji Torimura
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2018)
Article
Oncology
Shuichiro Nagata, Keiichi Mitsuyama, Hiroshi Kawano, Tetsuhiro Noda, Yasuhiko Maeyama, Michita Mukasa, Hidetoshi Takedatsu, Shinichiro Yoshioka, Kotaro Kuwaki, Jun Akiba, Osamu Tsuruta, Takuji Torimura
Article
Gastroenterology & Hepatology
Hideyuki Iwashita, Hidetoshi Takedatsu, Hiroyuki Murao, Sadahiro Funakoshi, Yasuhisa Kuniki, Satoshi Matsuoka, Shinji Tsukamoto, Masashi Yamaguchi, Satoshi Shakado, Teppei Kabemura, Shotaro Sakisaka
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
(2019)
Article
Multidisciplinary Sciences
Makoto Naganuma, Fumihito Hirai, Kiyonori Kobayashi, Kenji Watanabe, Ken Takeuchi, Nobuo Aoyama, Hiroshi Nozawa, Satoshi Motoye, Toshihide Ohmori, Akio Harada, Yushi Nagai, Takayuki Abe, Yoji Yamada, Katsutoshi Inagaki, Naoki Shimizu, Takanori Kanai, Mamoru Watanabe, Shinji Tanaka, Takehiro Arai, Masahiro Kishi, Chiyuki Watanabe, Yasuhisa Sakata, Toshifumi Ashida, Atsuo Maemoto, Souken Sai, Atsuo Kitano, Yukinori Sameshima, Kaoru Yokoyama, Taku Kobayashi, Takehisa Suekane, Koichiro Matsuda, Hirozumi Obata, Mikihiro Fujiya, Takayuki Shirai, Keiichi Tominaga, Hidetoshi Takedatsu, Ryouichi Suzuki
Article
Biochemistry & Molecular Biology
Hideto Sakisaka, Hidetoshi Takedatsu, Keiichi Mitsuyama, Shinichi Mochizuki, Kazuo Sakurai, Shotaro Sakisaka, Fumihito Hirai
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Gastroenterology & Hepatology
Shigeki Bamba, Ryosuke Sakemi, Toshimitsu Fujii, Teruyuki Takeda, Shin Fujioka, Kento Takenaka, Hiroki Kitamoto, Shotaro Umezawa, Hirotake Sakuraba, Toshihiro Inokuchi, Norimasa Fukata, Shinta Mizuno, Masaki Yamashita, Shinichiro Shinzaki, Hiroki Tanaka, Hidetoshi Takedatsu, Ryo Ozaki, Kei Moriya, Manabu Ishii, Tetsu Kinjo, Keiji Ozeki, Makoto Ooi, Ryohei Hayashi, Kazuki Kakimoto, Yuichi Shimodate, Kazuya Kitamura, Akihiro Yamada, Akira Sonoda, Yu Nishida, Kyouko Yoshioka, Shinya Ashizuka, Fumiaki Takahashi, Toshio Shimokawa, Taku Kobayashi, Akira Andoh, Toshifumi Hibi
JOURNAL OF GASTROENTEROLOGY
(2020)
Article
Physiology
Yaopeng Hu, Daniela Ross Kaschitza, Maria Essers, Prakash Arullampalam, Takayuki Fujita, Hugues Abriel, Ryuji Inoue
Summary: This study reveals the potential arrhythmogenicity of CaMKII-mediated TRPM4 channel overactivation linked to Ca2+ dysregulation in the heart, demonstrating the functional interaction between TRPM4 and CaMKII proteins. Enhanced by angiotensin II treatment, this novel arrhythmic mechanism involving excessive CaMKII activity causing TRPM4 overactivation in the stressed heart was further confirmed through experimental and numerical simulations.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2021)
Article
Nutrition & Dietetics
Haiyan Xu, Keizo Hiraishi, Lin-Hai Kurahara, Yuko Nakano-Narusawa, Xiaodong Li, Yaopeng Hu, Yoko Matsuda, Heping Zhang, Katsuya Hirano
Summary: The study found that the probiotic Lactobacillus rhamnosus M9 (Probio-M9) can suppress tumor formation in the large intestine by regulating the intestinal environment and ameliorating inflammation.
Article
Cell Biology
Yaopeng Hu, Qin Li, Lin-Hai Kurahara, Narumi Shioi, Keizo Hiraishi, Takayuki Fujita, Xin Zhu, Ryuji Inoue
Summary: The E7K mutation in the TRPM4 channel enhances channel function by altering its interaction with endogenous PIP2, potentially contributing to its arrhythmogenic potential.
Article
Biochemistry & Molecular Biology
Yaopeng Hu, Qin Li, Yanghua Shen, Takayuki Fujita, Xin Zhu, Ryuji Inoue
Summary: The E7K mutation leads to an increased open state of the TRPM4 channel, resulting in conduction disorders due to its pathologically enhanced activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
So Imakiire, Hidetoshi Takedatsu, Keiichi Mitsuyama, Hideto Sakisaka, Kozo Tsuruta, Masaru Morita, Nobuaki Kuno, Koichi Abe, Sadahiro Funakoshi, Hideki Ishibashi, Shinichiro Yoshioka, Takuji Torimura, Fumihito Hirai
Summary: This study found that PR3-ANCA measurement is useful for diagnosing UC, evaluating disease severity and extension, and predicting the clinical course.
Article
Medicine, General & Internal
Masaru Morita, Hidetoshi Takedatsu, Shinichiro Yoshioka, Keiichi Mitsuyama, Kozo Tsuruta, Kotaro Kuwaki, Ken Kato, Ryosuke Yasuda, Tatsuki Mizuochi, Yushiro Yamashita, Takumi Kawaguchi
Summary: The roles and methods of diagnostic colonoscopy in pediatric patients have evolved and adapted in recent years, as demonstrated by a retrospective analysis. The increasing prevalence of inflammatory bowel disease and eosinophilic gastrointestinal disorder emphasizes the importance of colonoscopy in infants and children.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Oncology
Hideki Ishibashi, Satoshi Nimura, Yoshiyuki Kayashima, Yasushi Takamatsu, Hiroki Iwasaki, Naohiko Harada, Seiya Momosaki, Hidenori Takedatsu, Shotaro Sakisaka, Morishige Takeshita
JOURNAL OF GASTROINTESTINAL ONCOLOGY
(2019)
Article
Gastroenterology & Hepatology
Lin Hai Kurahara, Keizo Hiraishi, Yaopeng Hu, Kaori Koga, Miki Onitsuka, Mayumi Doi, Kunihiko Aoyagi, Hidetoshi Takedatsu, Daibo Kojima, Yoshitaka Fujihara, Yuwen Jian, Ryuji Inoue
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2018)