Article
Engineering, Environmental
Liping Chen, Gengrui Wei, Yushen Zhang, Kaiying Wang, Cenchao Wang, Xuhan Deng, Yaqian Li, Xiaojing Xie, Jinling Chen, Fu Huang, Hang Chen, Bin Zhang, Chaohai Wei, Guanglei Qiu
Summary: Previous research has shown that Ca. Accumulibacter and Tetrasphaera play cooperative roles in enhanced biological phosphorus removal (EBPR). However, the utilization of fermentation products released by Tetrasphaera and their potential effects on Ca. Accumulibacter metabolism were unknown. This study investigated the metabolic details of Ca. Accumulibacter in using fermentation products for EBPR, providing insights into their interactions.
Article
Engineering, Environmental
Srdana Kolakovic, Elisabete B. Freitas, Maria A. M. Reis, Gilda Carvalho, Adrian Oehmen
Summary: This study investigated the relationship between the diversity of Accumulibacter and EBPR performance, revealing that changes in the community structure and conditions of Accumulibacter under different operating conditions can influence the success or failure of EBPR operation.
Article
Environmental Sciences
Srdana Kolakovic, Ricardo Salgado, Elisabete B. Freitas, Maria R. Bronze, Maja Turk Sekulic, Gilda Carvalho, Maria A. M. Reis, Adrian Oehmen
Summary: This study focused on the biodegradation of diclofenac in the enhanced biological phosphorus removal process, showing that polyphosphate accumulating organisms (PAOs) can contribute to the transformation of diclofenac into less toxic products. The study identified 23 transformation products and elucidated multiple metabolic pathways for diclofenac biotransformation, suggesting PAOs can complement the biodegradation carried out by other organisms in activated sludge.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Hongwei Sun, Xin Zhang, Feng Zhang, Hao Yang, Jianbo Lu, Shijian Ge, Xiaoqiang Li, Wei Zhang
Summary: The study demonstrates that free ammonia (FA) inhibits phosphorus release and uptake processes in enhanced biological phosphorus removal (EBPR) systems, affecting key microbial communities and Tetrasphaera being identified as the sole virtual biomarker for EBPR performance response to FA inhibition.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Engineering, Environmental
Jianfeng Zheng, Nan Wang, Ledan Zhao, Yajing Li, Jingjie Yu, Shaopo Wang
Summary: This study explored the microbial population dynamics of Accumulibacter (Acc) at low temperature and its metabolic shift to limiting polyphosphate (Poly-P) in the enhanced biological phosphorus removal (EBPR) system. The results showed that the microbial community structure changed at low temperature, and the formation of granular sludge in the EBPR system was enhanced. Additionally, the study found that poly-P levels can affect the metabolic pathway of Accumulibacter at low temperature.
WATER SCIENCE AND TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Sergio Tomas-Martinez, Hugo B. C. Kleikamp, Thomas R. Neu, Martin Pabst, David G. Weissbrodt, Mark C. M. van Loosdrecht, Yuemei Lin
Summary: This study highlights the importance of NulOs in non-pathogenic bacteria and their role in microbial ecosystems. Analysis using fluorescence lectin-binding, enzymatic quantification, and mass spectrometry revealed a diverse distribution of NulOs, including sialic acids and bacterial NulOs, in enriched microbial populations. Phylogenetic and proteomic analyses confirmed Candidatus Accumulibacter's capacity to produce and utilize different types of NulOs.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Varun N. Srinivasan, Guangyu Li, Dongqi Wang, Nicholas B. Tooker, Zihan Dai, Annalisa Onnis-Hayden, Charles Bott, Paul Dombrowski, Peter Schauer, Ameet Pinto, April Z. Gu
Summary: This study compared the microbial ecology of CAP between a side-stream EBPR and a conventional A2O EBPR process, revealing differences in microdiversity that likely contribute to the improved performance of S2EBPR. It also identified previously uncharacterized clades of CAP and demonstrated the temporally increasing predominance of certain CAP clades during the implementation of the S2EBPR configuration in full-scale EBPR systems.
Article
Environmental Sciences
Tingting Qian, Wei Sern Ong, Dan Lu, Yan Zhou
Summary: This study comprehensively investigates the form and transformation of released phosphorus from EBPR sludge biochar pyrolyzed at different temperatures. High-temperature EBPR sludge biochar containing a high proportion of Poly-P could be an ideal P fertilizer.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Engineering, Environmental
Guanglei Qiu, Yingyu Law, Rogelio Zuniga-Montanez, Xuhan Deng, Yang Lu, Samarpita Roy, Sara Swa Thi, Hui Yi Hoon, Thi Quynh Ngoc Nguyen, Kaliyamoorthy Eganathan, Xianghui Liu, Per H. Nielsen, Rohan B. H. Williams, Stefan Wuertz
Summary: Recent research has shown that enhanced biological phosphorus removal (EBPR) from municipal wastewater can be achieved at higher temperatures, around 30 degrees C and 35 degrees C. The study found that a slow-feeding strategy and sufficiently high carbon input are important for maintaining high and stable EBPR performance.
Article
Engineering, Environmental
A. Y. A. Mohamed, L. Welles, A. Siggins, M. G. Healy, D. Brdjanovic, A. M. Rada-Ariza, C. M. Lopez-Vazquez
Summary: This study investigated the effects of substrate composition and light intensity on the performance of a laboratory-scale EBPR-PAS system. Results showed that wastewater with high HCO3 and low NH4-N levels favored microalgal growth but had poor phosphorus removal, while adjusting the levels to balance PAOs and microalgae symbiosis positively influenced phosphorus removal. The system could operate without external aeration and achieve satisfactory phosphorus removal even at low light intensities.
Article
Ecology
Francesca Petriglieri, Caitlin Singleton, Miriam Peces, Jette F. Petersen, Marta Nierychlo, Per H. Nielsen
Summary: Members of the genus Dechloromonas play a significant role in enhanced biological phosphorus removal systems, with the potential for polyphosphate accumulation. Seven distinct Dechloromonas species were identified in different Danish EBPR plants, each capable of accumulating polyphosphate, glycogen, and polyhydroxyalkanoates.
Article
Environmental Sciences
Xiang Li, Shuting Shen, Yuye Xu, Ting Guo, Dai Hongliang, Xiwu Lu
Summary: Recovery of phosphorus from wastewater is important for establishing a new phosphorus cycle. The enhanced biological phosphorus removal process with sidestream phosphorus recovery (EBPR-SPR) is an effective method for removing and recovering phosphorus resources in wastewater. However, there is a lack of research on the transformation and fate of non-reactive phosphorus (NRP) in this process.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Ecology
C. M. Singleton, F. Petriglieri, K. Wasmund, M. Nierychlo, Z. Kondrotaite, J. F. Petersen, M. Peces, M. S. Dueholm, M. Wagner, P. H. Nielsen
Summary: The bacterial genus Tetrasphaera is responsible for enhanced biological phosphorus removal (EBPR) in wastewater treatment plants. Recent analyses showed that 16S rRNA genes cannot accurately resolve the lineage of Tetrasphaera, which actually consists of several different genera within the Dermatophilaceae. This study identifies and examines 14 metagenome-assembled genomes containing full-length 16S rRNA genes from wastewater treatment plants, and reveals that the uncultured Tetrasphaera clade 3 represents two distinct genera named Ca. Phosphoribacter and Ca. Lutibacillus. Ca. Phosphoribacter is found to be the most abundant former Tetrasphaera lineage and polyphosphate accumulating organisms (PAO) in EBPR systems in Denmark and globally.
Article
Engineering, Environmental
Ali Elahinik, Linghang Li, Martin Pabst, Ben Abbas, Dimitrios Xevgenos, Mark C. M. van Loosdrecht, Mario Pronk
Summary: This study investigated the conversion of glucose and its impact on phosphate removal by aerobic granular sludge. The results demonstrated that glucose could be used as a suitable substrate for achieving phosphate removal and that a microbial community consisting of fermentative organisms and polyphosphate accumulating organisms (PAO) developed.
Article
Agricultural Engineering
Jixiang Wang, Zejiao Li, Qian Wang, Zhongfang Lei, Tian Yuan, Kazuya Shimizu, Zhenya Zhang, Yasuhisa Adachi, Duu-Jong Lee, Rongzhi Chen
Summary: Enhanced biological phosphorus (P) removal (EBPR) through extracting P-rich liquid from aerobic granular sludge (AGS)-based sequencing batch reactors (SBRs) under no mixing during the anaerobic phase is proposed for sustainable wastewater treatment. Both tested bacterial AGS (BAGS) and algal-bacterial AGS (A-BAGS) systems showed stable low effluent P levels with little impact on organics and NH4+-N removal. The collected P-rich liquids from both systems have great potential for P recovery, mainly from influent P and P reserves in granules. This study suggests that AGS-based SBRs coupling Phostrip hold great potentials for P recovery and energy consumption reduction.
BIORESOURCE TECHNOLOGY
(2022)
Article
Microbiology
Travis C. Korosh, Andrew Dutcher, Brian F. Pfleger, Katherine D. McMahon
Article
Microbiology
Sarahi L. Garcia, Moritz Buck, Joshua J. Hamilton, Christian Wurzbacher, Hans-Peter Grossart, Katherine D. McMahon, Alexander Eiler
Article
Microbiology
Robin R. Rohwer, Joshua J. Hamilton, Ryan J. Newton, Katherine D. McMahon
Article
Biotechnology & Applied Microbiology
Jeffrey R. Dwulit-Smith, Joshua J. Hamilton, David M. Stevenson, Shaomei He, Ben O. Oyserman, Francisco Moya-Flores, Sarahi L. Garcia, Daniel Amador-Noguez, Katherine D. McMahon, Katrina T. Forest
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2018)
Article
Microbiology
Shaomei He, Maximilian P. Lau, Alexandra M. Linz, Eric E. Roden, Katherine D. McMahon
Article
Microbiology
Pamela Y. Camejo, Ben O. Oyserman, Katherine D. McMahon, Daniel R. Noguera
Editorial Material
Microbiology
Aaron Mitchell, Ira Blader, Patricia Bradford, Sarah D'Orazio, W. Paul Duprex, Craig D. Ellermeier, Ana Fernandez-Sesma, Michael J. Imperiale, Katherine McMahon, Marcela F. Pasetti, Susannah Tringe
Article
Limnology
Alexandra M. Linz, Frank O. Aylward, Stefan Bertilsson, Katherine D. McMahon
LIMNOLOGY AND OCEANOGRAPHY
(2020)
Article
Biochemistry & Molecular Biology
Sara Beier, Anders F. Andersson, Pierre E. Galand, Corentin Hochart, Juerg B. Logue, Katherine McMahon, Stefan Bertilsson
Article
Microbiology
Elizabeth A. McDaniel, Benjamin D. Peterson, Sarah L. R. Stevens, Patricia Q. Tran, Karthik Anantharaman, Katherine D. McMahon
Article
Microbiology
Sarahi L. Garcia, Maliheh Mehrshad, Moritz Buck, Jackson M. Tsuji, Josh D. Neufeld, Katherine D. McMahon, Stefan Bertilsson, Chris Greening, Sari Peura
Summary: By analyzing global metagenomes, the study identified the distribution and metabolic traits of Chlorobia in freshwater ecosystems, highlighting their importance in lake ecosystems. The findings contribute new insights into the biogeography and niche specialization of Chlorobia in lake environments.
Article
Microbiology
Christopher E. Lawson, Aniela B. Mundinger, Hanna Koch, Tyler B. Jacobson, Coty A. Weathersby, Mike S. M. Jetten, Martin Pabst, Daniel Amador-Noguez, Daniel R. Noguera, Katherine McMahon, Sebastian Lucker
Summary: This study reconstructed a genome-scale metabolic model for Nitrospira moscoviensis and experimentally validated the model predictions using proteomic analysis and C-13-tracer experiments combined with metabolomic analysis, revealing the central carbon metabolism of the bacteria and their utilization of formate as a carbon source. This metabolic model serves as a quantitative tool for understanding and predicting the metabolic processes of Nitrospira bacteria, contributing to the knowledge base of their metabolism for future studies.
Article
Microbiology
Benjamin D. D. Peterson, David P. P. Krabbenhoft, Katherine D. D. McMahon, Jacob M. M. Ogorek, Michael T. T. Tate, William H. H. Orem, Brett A. A. Poulin
Summary: The production of methylmercury (MeHg) is influenced by the bioavailability of inorganic divalent mercury (Hg(II)(i)) and the Hg-methylation capacity of the microbial community (controlled by the hgcAB gene cluster). However, the relative importance of these factors and their interaction in the environment is not well understood. Through metagenomic sequencing and a MeHg formation experiment, this study examined a wetland sulfate gradient to isolate the effects of each factor on MeHg formation. The bioavailability of Hg(II)(i) was correlated with dissolved organic matter composition, while the microbial Hg-methylation capacity was correlated with the abundance of hgcA genes. MeHg formation showed a synergistic response to both factors. Notably, hgcA sequences were found in diverse taxonomic groups, none of which contained genes for dissimilatory sulfate reduction. This research expands our understanding of the geochemical and microbial constraints on MeHg formation in natural environments and provides a framework for further mechanistic studies.
ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shaomei He, Alexandra M. Linz, Sarah L. R. Stevens, Patricia Q. Tran, Francisco Moya-Flores, Ben O. Oyserman, Jeffrey R. Dwulit-Smith, Katrina T. Forest, Katherine D. McMahon
Summary: Microbial rhodopsins are widely distributed in aquatic environments and may significantly contribute to phototrophy and energy budgets in global oceans. The study explored the diversity, distribution, and expression of opsin genes in freshwater lakes with different characteristics. The results showed that opsin genes occurred more frequently in clearwater lakes, and their distribution was influenced by lake optical properties and dissolved organic carbon (DOC) levels. Gene expression analysis confirmed the significance of rhodopsin-based phototrophy in clearwater lakes. Overall, these findings highlight the importance of rhodopsin-based phototrophy in freshwater energy budgets.
Article
Microbiology
Patricia Q. Tran, Samantha C. Bachand, C. Jacob, Kristopher Kieft, Elizabeth A. McDaniel, Katherine D. McMahon, Karthik Anantharaman
Summary: This study investigates the ecological significance of cysteine degradation in producing hydrogen sulfide (H2S), a toxic compound found in freshwater lakes. The researchers identified bacterial strains from a lake that can produce H2S in the presence of oxygen, highlighting the importance of this process in natural ecosystems.
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.