Article
Biochemistry & Molecular Biology
Lu Xiao, Guoxia Liu, Fuyu Gong, Zhen Cai, Yin Li
Summary: This study investigated the reductive carboxylation activities of six heterotetrameric PFORs from hyperthermophilic archaea, revealing diversity in their functions. Among them, PFORpfm from Pyrolobus fumarii showed the highest reductive carboxylation activities and red/ox ratio, indicating the potential significance of PFOR in autotrophic CO2 fixation in high temperature environments.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Natalie Payne, Arlette Kpebe, Chloe Guendon, Carole Baffert, Julien Ros, Regine Lebrun, Yann Denis, Laetitia Shintu, Myriam Brugna
Summary: Through studying Desulfovibrio fructosovorans, we discovered that Hnd is involved in ethanol metabolism and oxidizes H-2 via its electron bifurcation mechanism, producing NADH and reduced ferredoxin.
MOLECULAR MICROBIOLOGY
(2022)
Article
Agricultural Engineering
Yunxia Qin, Jia Wang, Yongjun Fang, Jilai Lu, Xiangyu Shi, Jianghua Yang, Xiaohu Xiao, Xuehua Luo, Xiangyu Long
Summary: Research has shown that anaerobic metabolism plays a crucial role in rubber biosynthesis in laticifers of Hevea brasiliensis. The key genes HbLDH1 and HbPDC4 in anaerobic metabolism are activated during tapping, resulting in the production of acetaldehyde and ATP through ethanol and lactate fermentation, which contribute to rubber synthesis.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Environmental Sciences
Kartik Dhar, Sudharsanam Abinandan, Tanmoy Sana, Kadiyala Venkateswarlu, Mallavarapu Megharaj
Summary: Our study examined the anaerobic microbial degradation of hazardous polycyclic aromatic hydrocarbons (PAHs) and found that sulfate-reducing bacteria (SRB) in contaminated freshwater sediments can effectively degrade three-ringed phenanthrene and highly recalcitrant four-ringed pyrene. Desulfovibrio was the dominant bacterium in the phenanthrene-degrading culture, while Rhodopseudomonas was a significant member in the pyrene-degrading culture. This research indicates that SRB could play a crucial role in the natural attenuation of PAHs in oxygen-depleted freshwater sediments.
ENVIRONMENTAL RESEARCH
(2023)
Article
Microbiology
Arlette Kpebe, Chloe Guendon, Natalie Payne, Julien Ros, Manel Khelil Berbar, Regine Lebrun, Carole Baffert, Laetitia Shintu, Myriam Brugna
Summary: This study confirms the role of Hnd hydrogenase in ethanol metabolism and uncovers an essential function for a Desulfovibrio hydrogenase for the first time. Hnd is required for S. fructosivorans growth on ethanol, and it produces H-2 from NADH and reduced ferredoxin for ethanol conversion.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Min Kyoung Cho, Ling Jin, Jung Ho Han, Jung-Suk Jin, Se-Yun Cheon, Su Shin, Sung-Jin Bae, Jang-Kyung Park, Ki-Tae Ha
Summary: Endometriosis is a chronic inflammatory disorder with limited treatment options. However, recent studies have shown that Prunella vulgaris, a traditional herbal medicine with anti-estrogenic effects, may be a potential treatment for endometriosis. In this study, water-extracted Prunella vulgaris was found to alleviate endometriosis in mice by inducing apoptosis in endometrial cells through the regulation of aerobic glycolysis enzymes. These findings suggest that Prunella vulgaris may be a promising candidate for the prevention and treatment of endometriosis.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Environmental Sciences
Bruna Del Busso Zampieri, Elis Watanabe Nogueira, Ana Julia Fernandes Cardoso de Oliveira, Irene Sanchez-Andrea, Gunther Brucha
Summary: The study analyzed the influence of metals on the activity and composition of sulfate-reducing bacteria (SRB), finding that an increase in metal concentration led to decreased SRB activity. The more toxic metals, such as Cd and Cu, were associated with less SRB and more fermenters in the microbial community, while less toxic metals like Cr and Zn had a higher abundance of SRB genera. An isolated Desulfovibrio species may have implications for future bioremediation studies in neutral pH effluents contaminated by metals.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Microbiology
Yijun Zhu, Mohammed Dwidar, Ina Nemet, Jennifer A. Buffa, Naseer Sangwan, Xinmin S. Li, James T. Anderson, Kymberleigh A. Romano, Xiaoming Fu, Masanori Funabashi, Zeneng Wang, Pooja Keranahalli, Shawna Battle, Aaron N. Tittle, Adeline M. Hajjar, Valentin Gogonea, Michael A. Fischbach, Joseph A. DiDonato, Stanley L. Hazen
Summary: Recent studies have shown the critical role of gut microbiota in metabolizing dietary phenylalanine into phenylacetic acid (PAA), which is important for the production of phenylacetylglutamine (PAGln) linked to atherosclerotic cardio-vascular disease (ASCVD). Researchers have identified two different microbial pathways, involving phenylpyruvate:ferre-doxin oxidoreductase (PPFOR) and phenylpyruvate decarboxylase (PPDC), for PAA formation in the gut. The abundance of these pathways is significantly higher in ASCVD patients compared to controls. These findings provide insights into the therapeutic targeting of PAGln formation in vivo.
CELL HOST & MICROBE
(2023)
Article
Microbiology
Jing Wang, Xiaohong Li, Fang Guan, Zhibo Yang, Xiaofan Zhai, Yimeng Zhang, Xuexi Tang, Jizhou Duan, Hui Xiao
Summary: In this study, culturable sulfate-reducing bacteria (SRB) were isolated and identified from the inner rust layer of steel and sediments. A comparison of amino acid sequences encoding related enzymes in the sulfate reduction pathway was conducted between anaerobic and facultative anaerobic SRB strains. The study found differences in the distribution of enzymes between the two types of SRB, providing insights into the metabolic mechanisms and potential applications of SRB.
Article
Biology
Laura Prioretti, Giulia D'Ermo, Pascale Infossi, Arlette Kpebe, Regine Lebrun, Marielle Bauzan, Elisabeth Lojou, Bruno Guigliarelli, Marie-Therese Giudici-Orticoni, Marianne Guiral
Summary: Aquifex aeolicus is a microaerophilic bacterium that oxidizes hydrogen and sulfur and assimilates CO2 via the reverse tricarboxylic acid cycle (rTCA). It possesses pentameric versions of key enzymes (PFOR and OGOR) involved in this pathway, which are highly abundant in the cell. Two low redox potential and oxygen-stable ferredoxins (Fd6 and Fd7) were identified in A. aeolicus and shown to interact with PFOR and OGOR, potentially serving as electron donors in vivo.
Article
Engineering, Environmental
Rafael Marcal Ferraz, Josiel Martins Costa, Livia Martins Verola, Giselle Patricia Sancinetti, Renata Piacentini Rodriguez
Summary: This study evaluated the efficiency of anaerobic sequencing batch reactors in treating sulfate-rich wastewater using different inocula sources and immobilization methods. The results showed high sulfate removal rates in both lactate and ethanol phases, with an increase in sulfate-reducing bacteria and methanogenic Archaea abundance. Understanding the effects of electron donors, inoculum origin, and immobilization on sulfate reduction and microbial community development can aid in establishing strategies for bioremediation of acid mine drainage.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Immunology
Kairuo Wang, Yixuan Guo, Yuanyuan Liu, Xiao Cui, Xiang Gu, Lixiang Li, Yanqing Li, Ming Li
Summary: This study found that the deficiency of F. prausnitzii in the gut is associated with elevated F. prausnitzii-reactive IgA in ulcerative colitis (UC) patients. Forced immunization of rabbits with F. prausnitzii led to intestinal inflammation. PFOR was identified as an IgA-binding antigen of F. prausnitzii and its immunoreactivity was validated in peripheral blood mononuclear cells (PBMCs), which showed elevated expression of inflammatory cytokines. Phylogenetic analysis revealed that PFOR is a common but conserved protein among gut bacteria.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Engineering, Chemical
Ivan Kushkevych, Aneta Kovarova, Dani Dordevic, Jonah Gaine, Peter Kollar, Monika Vitezova, Simon K. -M. R. Rittmann
Summary: This review discusses the importance of sulfate-reducing bacteria (SRB) in producing hydrogen sulfide and their cryopreservation methods. It highlights the factors affecting microbial viability after cryopreservation and recommends storing SRB in glass vials with low salt concentration. Furthermore, it emphasizes the potential impact of SRB in medical, construction, and environmental sectors from a molecular biological and bioengineering perspective.
Article
Immunology
Sudha B. Singh, Cristina N. Coffman, Amanda Carroll-Portillo, Matthew G. Varga, Henry C. Lin
Summary: Sulfate Reducing Bacteria (SRB) are often increased in inflammatory conditions and can activate the Notch signaling pathway; Desulfovibrio (DSV) can induce the expression of Notch-related proteins in macrophages and epithelial cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Jun Li, Salma Tabassum
Summary: The study effectively improved the high concentration sulfate removal from sewage wastewater using an acid production-sulfate reduction reaction system. The two-phase anaerobic process showed successful results with over 40% COD removal efficiency in the acid-producing phase, demonstrating good synergistic effects between acid-producing bacteria and sulfate-reducing bacteria.
CHEMICAL ENGINEERING JOURNAL
(2022)
Editorial Material
Environmental Sciences
Mireille Ansaldi, Ingrid Bazin, Pierre Cholat, Agnes Rodrigue, David Pignol
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2017)
Article
Environmental Sciences
Elodie C. T. Descamps, Damien Meunier, Catherine Brutesco, Sandra Preveral, Nathalie Franche, Ingrid Bazin, Bertrand Miclot, Philippe Larosa, Camille Escoffier, Jean-Raphael Fantino, Daniel Garcia, Mireille Ansaldi, Agnes Rodrigue, David Pignol, Pierre Cholat, Nicolas Ginet
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2017)
Article
Environmental Sciences
Nathalie Franche, Manon Vinay, Mireille Ansaldi
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2017)
Article
Virology
Clara Torres-Barcelo, Oliver Kaltz, Remy Froissart, Sylvain Gandon, Nicolas Ginet, Mireille Ansaldi
Article
Virology
Mireille Ansaldi, Laurent Debarbieux, Sylvain Gandon, Marie-Agnes Petit, Paulo Tavares, Pascale Boulanger
Article
Multidisciplinary Sciences
Manon Vinay, Nathalie Franche, Gerald Gregori, Jean-Raphael Fantino, Flavie Pouillot, Mireille Ansaldi
Review
Biochemistry & Molecular Biology
Astrid Wahl, Aurelia Battesti, Mireille Ansaldi
MOLECULAR MICROBIOLOGY
(2019)
Article
Microbiology
David R. Olivenza, Josep Casadesus, Mireille Ansaldi
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Virology
Fernando Clavijo-Coppens, Nicolas Ginet, Sophie Cesbron, Martial Briand, Marie-Agnes Jacques, Mireille Ansaldi
Summary: Xylella fastidiosa is an emerging plant pathogen that has caused recent epidemics in southern Europe, leading researchers to explore alternative treatments such as phage therapy to control its spread and cure infected crops. Through isolation and characterization of various phages, including two new phage species, scientists were able to propose new taxonomic elements and make significant progress in understanding and combatting this pathogen.
Article
Biochemistry & Molecular Biology
Julia Gendre, Mireille Ansaldi, David R. Olivenza, Yann Denis, Josep Casadesus, Nicolas Ginet
Summary: In this study, we aimed to genetically characterize and functionally annotate 10 new Salmonella phage genomes isolated in Spain, with a focus on identifying potential functions for phage therapy. The results of the study are important for the biocontrol of Salmonella.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Annalisa Pierro, Alessio Bonucci, Davide Normanno, Mireille Ansaldi, Eric Pilet, Olivier Ouari, Bruno Guigliarelli, Emilien Etienne, Guillaume Gerbaud, Axel Magalon, Valerie Belle, Elisabetta Mileo
Summary: The study focused on investigating protein dynamics in complex environments such as cells, utilizing electron paramagnetic resonance spectroscopy coupled to site-directed spin labeling (SDSL-EPR). The research demonstrated the feasibility of studying protein local dynamics while maintaining cell integrity and protein activity. The dynamics of the chaperone NarJ in its natural host, Escherichia coli, were explored, revealing that the cellular medium impacts NarJ's structural dynamics in a site-specific manner while preserving the protein's flexibility.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Review
Virology
Mireille Ansaldi, Pascale Boulanger, Charlotte Brives, Laurent Debarbieux, Nicolas Dufour, Remy Froissart, Sylvain Gandon, Claire Le Henaff, Marie-Agnes Petit, Eduardo Rocha, Clara Torres-Barcelo
Review
Virology
Mireille Ansaldi, Pascale Boulanger, Charlotte Brives, Laurent Debarbieux, Nicolas Dufour, Remy Froissart, Sylvain Gandon, Claire Le Henaff, Marie-Agnes Petit, Eduardo Rocha, Clara Torres-Barcelo
Review
Microbiology
Rachid Menouni, Geoffrey Hutinet, Marie-Agnes Petit, Mireille Ansaldi
FEMS MICROBIOLOGY LETTERS
(2015)