Article
Infectious Diseases
Aryan Rahimi-Midani, Seon-Woo Lee, Tae-Jin Choi
Summary: Bacteriophages are viruses that infect bacterial hosts, playing a crucial role in modern biotechnology and combating antibiotic-resistant microbes. With the advancement of sequencing technologies, their application is being reconsidered, although new challenges need to be addressed. More studies are required to find low-risk control methods against antimicrobial-resistant bacteria, as phage therapy is not expected to be the sole strategy.
Review
Biochemistry & Molecular Biology
Jose Luis Villalpando-Aguilar, Gilberto Matos-Pech, Itzel Lopez-Rosas, Hugo Gildardo Castelan-Sanchez, Fulgencio Alatorre-Cobos
Summary: Phage therapy is an alternative treatment that uses bacteriophages to kill specific bacteria. It has shown positive results in improving food production in agriculture. However, finding effective bacteriophages is crucial for the success of this therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Golshan Shakeri, Jens A. Hammerl, Abdollah Jamshidi, Kiarash Ghazvini, Manfred Rohde, Istvan Szabo, Corinna Kehrenberg, Madeleine Plotz, Sophie Kittler
Summary: This study isolated and characterized six bacteriophages that infect food-derived Salmonella isolates, with phage VB_StyS-LmqsSP1 showing the potential for biocontrol by suppressing Salmonella growth and reducing bacterial titer. The phage demonstrated lytic efficacy under conditions prevalent in food production environments, making it a promising candidate for controlling Salmonella contamination on poultry.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Lu Meng, Fengmin Yang, Yan Pang, Zhenping Cao, Feng Wu, Deyue Yan, Jinyao Liu
Summary: This study demonstrates the use of cationic polymers to selectively cap bacteriophages, enabling the entry of phages into intestinal epithelial cells and escaping from endosomes. This nanocapping technique significantly reduces the translocation of pathogens and shows potential for developing next-generation antimicrobials.
Article
Virology
Hugo Oliveira, Rita Domingues, Benjamin Evans, J. Mark Sutton, Evelien M. Adriaenssens, Dann Turner
Summary: The genetic diversity of Acinetobacter phages was explored using comparative genomics approaches, revealing eight clusters and 46 sub-clusters. Five new subfamilies were proposed, and a reorganisation of the genus Obolenskvirus was suggested. These findings provide updated insights into the viruses infecting Acinetobacter species and their diversity.
Article
Biochemistry & Molecular Biology
Paulina Sliwka, Beata Weber-Dabrowska, Maciej Zaczek, Marta Kuzminska-Bajor, Izabela Dusza, Aneta Skaradzinska
Summary: This study characterized three bacteriophages (BF9, BF15, and BF17) with lytic activity against ESBLs- and AmpC-producing E. coli. Comparative genomic and phylogenetic analysis identified these phages as belonging to the Dhillonvirus, Tequatrovirus, and Asteriusvirus genera, respectively. The phages inhibited bacterial growth and retained their lytic ability at a wide range of temperatures (-20-40 degrees C) and pH values (5-9). The absence of toxin genes and bacterial virulence factors in BF9, BF15, and BF17 further supports their potential for future phage applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Prasanna Mutusamy, Kirnpal Kaur Banga Singh, Lee Su Yin, Bent Petersen, Thomas Sicheritz-Ponten, Martha R. J. Clokie, Stella Loke, Andrew Millard, Sivachandran Parimannan, Heera Rajandas
Summary: Salmonella infections are becoming harder to control due to the emergence of multidrug-resistant strains. Researchers have found new Salmonella-specific phages from a rainforest, which have potential as an alternative treatment for these infections. The phages show differences in their lytic spectrum and pH stability, possibly due to nucleotide sequence variations in certain proteins. This study highlights the diversity of novel Salmonella phages in rainforest regions and their potential as antimicrobial agents against multidrug-resistant strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Brieuc Van Nieuwenhuyse, Dimitri Van der Linden, Olga Chatzis, Cedric Lood, Jeroen Wagemans, Rob Lavigne, Kaat Schroven, Jan Paeshuyse, Catherine de Magnee, Etienne Sokal, Xavier Stephenne, Isabelle Scheers, Hector Rodriguez-Villalobos, Sarah Djebara, Maya Merabishvili, Patrick Soentjens, Jean-Paul Pirnay
Summary: In this study, the authors use a combination of bacteriophage and antibiotic therapy to treat extensively drug-resistant Pseudomonas aeruginosa infection in a toddler post liver transplantation. They report on the clinical and microbiological improvement and investigate the reasons why bacterial phage resistance did not lead to therapeutic failure.
NATURE COMMUNICATIONS
(2022)
Article
Virology
Marta Kuzminska-Bajor, Paulina Sliwka, Maciej Ugorski, Pawel Korzeniowski, Aneta Skaradzinska, Maciej Kuczkowski, Magdalena Narajaczyk, Alina Wieliczko, Rafal Kolenda
Summary: Five novel lytic Salmonella phages, UPWr_S1-5, were isolated and characterized in this study. They exhibit efficient infection of various Salmonella serovars and show potential in combating Salmonella infections, making them promising tools for biological control of salmonellosis.
Article
Virology
Carlos E. Martinez-Soto, Stevan Cucic, Janet T. Lin, Sarah Kirst, El Sayed Mahmoud, Cezar M. Khursigara, Hany Anany
Summary: This study quantitatively determined the host range profiles of isolated phages against three different bacterial genera using a high throughput turbidimetric protocol and analyzed the data with an accessible analytic tool PHIDA. The results showed high variability in the host range index (HRi) among Listeria, Salmonella, and Pseudomonas phages isolated with different enrichment methods, with no direct correlation found between the phage host range breadth and the enrichment method. The high throughput method and analytics tool developed in this study can be easily adapted to any phage study and provide a consensus for phage host range determination.
Review
Food Science & Technology
Nitin Dhowlaghar, Thomas G. Denes
Summary: Bacteriophage products are gaining interest in controlling foodborne pathogens, but residual phages could impact bacterial recovery and efficacy. Including appropriate controls and methods can mitigate these impacts.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biochemistry & Molecular Biology
Spyros Tastsoglou, Giorgos Skoufos, Marios Miliotis, Dimitra Karagkouni, Ioannis Koutsoukos, Anna Karavangeli, Filippos S. Kardaras, Artemis G. Hatzigeorgiou
Summary: DIANA-miRPath is an online miRNA analysis platform that allows exploration of combined miRNA effects through predicted or experimentally supported miRNA interactions. Its latest version introduces a customizable target-based miRNA functional analysis engine, enriched with interaction, annotation, and parameterization options. The platform integrates various datasets and enables enrichment analysis on GO terms, pathways, and expression data for a wide range of states. Additionally, it provides a module for utilizing CRISPR knock-out screen datasets to identify miRNAs with potentially crucial roles.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Virology
Jean Pierre Gonzalez-Gomez, Osvaldo Lopez-Cuevas, Nohelia Castro-del Campo, Irvin Gonzalez-Lopez, Celida Isabel Martinez-Rodriguez, Bruno Gomez-Gil, Cristobal Chaidez
Summary: Two lytic Vibrio phages (vB_VpaP_AL-1 and vB_VpaS_AL-2) isolated from estuary water in Sinaloa, Mexico were found to be potential biocontrol agents against AHPND-causing V. parahaemolyticus.
Review
Biochemistry & Molecular Biology
Abdallah Abdelsattar, Alyaa Dawoud, Salsabil Makky, Rana Nofal, Ramy Aziz, Ayman El-Shibiny
Summary: This article summarizes the applications of bacteriophages in various fields and discusses the limitations and knowledge gaps in the field. Additionally, the computational methods used in phage genome analysis are critically assessed, providing useful insights for researchers in selecting suitable approaches for phage-related research aims and applications.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2022)
Article
Microbiology
Yan D. Niu, Hui Liu, Hechao Du, Ruiqiang Meng, El Sayed Mahmoud, Guihua Wang, Tim A. McAllister, Kim Stanford
Summary: In this study, the efficacy of different bacteriophages against E. coli O157 was evaluated, revealing that the combination of phages did not always follow the individual phage activity. Factors such as temperature, exposure time, and phage strains influenced the interactions among phages, with some combinations showing facilitation while others demonstrated antagonistic effects. The development of phage cocktails should consider confirming lack of antagonism and preferably facilitation or synergistic effects among constituent phages.
FRONTIERS IN MICROBIOLOGY
(2021)
Editorial Material
Environmental Sciences
Mireille Ansaldi, Ingrid Bazin, Pierre Cholat, Agnes Rodrigue, David Pignol
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2017)
Article
Environmental Sciences
Elodie C. T. Descamps, Damien Meunier, Catherine Brutesco, Sandra Preveral, Nathalie Franche, Ingrid Bazin, Bertrand Miclot, Philippe Larosa, Camille Escoffier, Jean-Raphael Fantino, Daniel Garcia, Mireille Ansaldi, Agnes Rodrigue, David Pignol, Pierre Cholat, Nicolas Ginet
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2017)
Article
Virology
Mireille Ansaldi, Laurent Debarbieux, Sylvain Gandon, Marie-Agnes Petit, Paulo Tavares, Pascale Boulanger
Article
Microbiology
Caroline L. Monteil, Guy Perriere, Nicolas Menguy, Nicolas Ginet, Beatrice Alonso, Nicolas Waisbord, Stephane Cruveiller, David Pignol, Christopher T. Lefevre
ENVIRONMENTAL MICROBIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Astrid Wahl, Aurelia Battesti, Mireille Ansaldi
MOLECULAR MICROBIOLOGY
(2019)
Article
Microbiology
David R. Olivenza, Josep Casadesus, Mireille Ansaldi
ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Ecology
Caroline L. Monteil, Denis S. Grouzdev, Guy Perriere, Beatrice Alonso, Zoe Rouy, Stephane Cruveiller, Nicolas Ginet, David Pignol, Christopher T. Lefevre
Article
Ecology
Caroline L. Monteil, Karim Benzerara, Nicolas Menguy, Cecile C. Bidaud, Emmanuel Michot-Achdjian, Romain Bolzoni, Francois P. Mathon, Margot Coutaud, Beatrice Alonso, Camille Garau, Didier Jezequel, Eric Viollier, Nicolas Ginet, Magali Floriani, Sufal Swaraj, Martin Sachse, Vincent Busigny, Elodie Duprat, Francois Guyot, Christopher T. Lefevre
Summary: Bacteria can synthesize a variety of non-biological precipitates with different chemical compositions and structures within cells, including magnetic particles and calcium carbonate granules. Research has shown that the amorphous calcium carbonate granules formed by these bacteria are contained within membrane-delimited vesicles. Analysis of a new genus of Alphaproteobacteria revealed atypical structure of the magnetosome gene cluster, and the bacteria consume energy to maintain an environment suitable for calcium carbonate granule formation.
Article
Virology
Fernando Clavijo-Coppens, Nicolas Ginet, Sophie Cesbron, Martial Briand, Marie-Agnes Jacques, Mireille Ansaldi
Summary: Xylella fastidiosa is an emerging plant pathogen that has caused recent epidemics in southern Europe, leading researchers to explore alternative treatments such as phage therapy to control its spread and cure infected crops. Through isolation and characterization of various phages, including two new phage species, scientists were able to propose new taxonomic elements and make significant progress in understanding and combatting this pathogen.
Article
Chemistry, Multidisciplinary
Annalisa Pierro, Alessio Bonucci, Davide Normanno, Mireille Ansaldi, Eric Pilet, Olivier Ouari, Bruno Guigliarelli, Emilien Etienne, Guillaume Gerbaud, Axel Magalon, Valerie Belle, Elisabetta Mileo
Summary: The study focused on investigating protein dynamics in complex environments such as cells, utilizing electron paramagnetic resonance spectroscopy coupled to site-directed spin labeling (SDSL-EPR). The research demonstrated the feasibility of studying protein local dynamics while maintaining cell integrity and protein activity. The dynamics of the chaperone NarJ in its natural host, Escherichia coli, were explored, revealing that the cellular medium impacts NarJ's structural dynamics in a site-specific manner while preserving the protein's flexibility.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Review
Virology
Mireille Ansaldi, Pascale Boulanger, Charlotte Brives, Laurent Debarbieux, Nicolas Dufour, Remy Froissart, Sylvain Gandon, Claire Le Henaff, Marie-Agnes Petit, Eduardo Rocha, Clara Torres-Barcelo
Review
Virology
Mireille Ansaldi, Pascale Boulanger, Charlotte Brives, Laurent Debarbieux, Nicolas Dufour, Remy Froissart, Sylvain Gandon, Claire Le Henaff, Marie-Agnes Petit, Eduardo Rocha, Clara Torres-Barcelo