Article
Veterinary Sciences
Kayla M. Even, Angela M. Gaesser, Sarah A. Ciamillo, Renata L. Linardi, Kyla F. Ortved
Summary: Joint injury often leads to cartilage damage and posttraumatic inflammation, which can eventually cause osteoarthritis. This study aims to compare the immunomodulatory properties of bone marrow-derived mesenchymal stem cells (BM-MSCs) culture-expanded in different media supplemented with xenogen-free substitutes. The findings suggest that BM-MSCs cultured in equine serum (ES) demonstrate enhanced immunomodulatory abilities, while media containing platelet lysate (PL) may have therapeutic potential due to higher concentrations of growth factors. Further research is needed to determine the optimal xenogen-free supplement for optimizing BM-MSC performance.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ayano Hatori, Daiki Yamakawa, Sarah Al-Maawi, Eva Dohle, Jin Chikira, Yasuyuki Fujii, Megumu Miki, Robert Sader, Daichi Chikazu, Shahram Ghanaati, Yoko Kawase-Koga
Summary: This study investigates the use of PRF-conditioned medium (CM) in enhancing the osteogenic potential of DPSCs. Compared to FBS culture, DPSCs cultured with PRF-CM exhibit superior calcification levels but inferior proliferation rates.
BIOENGINEERING-BASEL
(2023)
Article
Agriculture, Dairy & Animal Science
Usman Rashid, Evelyn Saba, Arfan Yousaf, Waleed Ahsan Tareen, Adeel Sarfraz, Man Hee Rhee, Mansur Abdullah Sandhu
Summary: The use of fetal bovine serum (FBS) in regenerative medicine has ethical and scientific concerns. This study found that autologous platelet lysate (A-PL) could be used as an alternative to FBS for culturing canine mesenchymal stem cells (cMSCs) without negatively affecting their proliferation and viability. Furthermore, the A-PL enhanced the genetic potential of the cMSCs for multilineage differentiation.
Article
Biotechnology & Applied Microbiology
Marline Kirsch, Jessica Rach, Wiebke Handke, Axel Seltsam, Iliyana Pepelanova, Sarah Strauss, Peter Vogt, Thomas Scheper, Antonina Lavrentieva
Summary: In this study, human adipose tissue-derived MSCs cultured with human platelet lysate (hPL) showed enhanced proliferation and differentiation compared to those cultured with human serum (HS) or fetal calf serum (FCS) in 2D. Additionally, the use of hPL in GelMA hydrogels for 3D culture led to increased cell spreading and proliferation, especially in combination with hPL-supplemented media. These findings highlight the potential benefits of hPL as a supplement for MSC expansion and differentiation in both 2D and 3D cultures.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yu-Hsun Chang, Kun-Chi Wu, Dah-Ching Ding
Summary: This study found that Exo(-)FBS-cultured HUCMSCs have similar characteristics to FBS-cultured HUCMSCs and showed better therapeutic effects in a mouse osteoarthritis model. These findings provide a basis for future clinical trials using Exo(-)FBS-cultured stem cells for the treatment of osteoarthritis.
Article
Biology
Yedidya Zagury, Iris Ianovici, Shira Landau, Neta Lavon, Shulamit Levenberg
Summary: Cultured meat can be a sustainable and more ethical alternative to conventional meat. This study focuses on the development of cultured fat tissue, an essential component in meat, and successfully integrates it with cultured muscle tissue, mimicking the structure of marbled meat.
COMMUNICATIONS BIOLOGY
(2022)
Review
Cell & Tissue Engineering
Silvia Palombella, Carlotta Perucca Orfei, Greta Castellini, Silvia Gianola, Silvia Lopa, Maddalena Mastrogiacomo, Matteo Moretti, Laura de Girolamo
Summary: This systematic review analyzes the effect of Human Platelet Lysate (HPL) and Fetal Bovine Serum (FBS) on Bone Marrow Mesenchymal Stem Cells (BMSCs) and Adipose-derived Stem Cells (ASCs). The results show that cells cultured with HPL have higher proliferation rate and shorter doubling time compared to cells cultured with FBS. Additionally, HPL increases the secretion of Vascular Endothelial Growth Factor (VEGF) and maintains the immunomodulatory abilities of both cell types.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Anatomy & Morphology
Caiyun Ma, Yang Liu, Yingchun Ma, Lijie Jiang, Qianyi Huang, Gaofeng Liu, Yu Guo, Chunjing Wang, Changqing Liu
Summary: This study successfully isolated PMSCs from Sprague-Dawley rat fetal lung tissue for the first time and demonstrated their stemness and multi-differentiation potentials, suggesting a potential regenerative cell resource for cell regenerative therapy of lung injury.
Article
Biochemistry & Molecular Biology
Aika Sawada, Takuya Yamamoto, Takahiko Sato
Summary: Exosomes in serum can inhibit the differentiation of myogenic cells, which is associated with the downregulation of Tceal5 and Tceal7 genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Eleanor Luce, Clara Steichen, Soumeya Abed, Anne Weber, Philippe Leboulch, Leila Maouche-Chretien, Anne Dubart-Kupperschmitt
Summary: The study demonstrates that siPSCs can differentiate into liver cells and self-organize into 3D structures, facilitating research on liver development and the development of co-culture systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Jia Wang, Yue Sun, Yiping Liu, Jize Yu, Xiaolin Sun, Lin Wang, Yanmin Zhou
Summary: This study investigated the existence of MSCs in Schneiderian membrane and the effects of PRF on these cells. The results showed that MSCs existed in Schneiderian membrane and PRF stimulation promoted cell proliferation, migration, and osteogenic differentiation. The application of PRF in maxillary sinus floor elevation could accelerate bone healing and increase the quantity and quality of new bone.
CELL COMMUNICATION AND SIGNALING
(2022)
Article
Multidisciplinary Sciences
Ferdy K. Cayami, Lauria Claeys, Ruben de Ruiter, Bernard J. Smilde, Lisanne Wisse, Natalija Bogunovic, Elise Riesebos, Lyra Eken, Irsan Kooi, Erik A. Sistermans, Nathalie Bravenboer, Gerard Pals, Sultana M. H. Faradz, Daoud Sie, E. Marelise W. Eekhoff, Dimitra Micha
Summary: We developed a highly efficient platelet lysate-based approach to directly transdifferentiate skin-derived human fibroblasts to osteoblast-like cells. The transdifferentiated cells showed increased expression of osteoblast-specific markers and exhibited osteogenic properties. RNA-seq analysis revealed distinct transcriptome profiles between the transdifferentiated cells and primary fibroblasts. This in vitro model may contribute to studying osteoblast-dependent disorders in patient-derived cells.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Gai Xue, JinHu Zhang, LiYing Wu, Shuang Sun, HongHai Wu, YanNing Hou, JinXuan Wang
Summary: The aim of this study was to investigate the effect of a simulated liver tissue injury microenvironment on the directed differentiation of umbilical cord mesenchymal stem cells into hepatocytes with CYP450 metabolic activity in vitro. The results showed that a liver injury microenvironment is conducive for the directed differentiation of umbilical cord mesenchymal stem cells into hepatocytes with metabolic enzyme activity.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Veterinary Sciences
Cara R. Pilgrim, Kiera A. McCahill, Jenna G. Rops, Jaustin M. Dufour, Keith A. Russell, Thomas G. Koch
Summary: Despite concerns over the use of fetal bovine serum (FBS), it is still widely used in cell culture media for isolating and expanding mesenchymal stromal cells (MSCs). Research on alternative supplements and serum-free media has been conducted, and while none of them can completely replace FBS, short-term or transitional use of FBS-free media can be equally effective.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Medicine, Research & Experimental
Santosh Gupta, Akriti Sharma, Sugan Paneerselvan, Sangeetha Kandoi, Bamadeb Patra, Dillip Kumar Bishi, Rama Shanker Verma
Summary: Liver failure is a critical disease and regenerative therapies using cell-based approaches are still being explored. This study presents a novel method of engineering hepatocyte-like cells using serum from hyperbilirubin patients and demonstrates their potential for improving liver function and histological changes in an acute liver injury rat model.
XENOTRANSPLANTATION
(2022)
Article
Surgery
Shaghayegh Arasteh, Sayeh Khanjani, Hannaneh Golshahi, Sahba Mobini, Masoud Taghizadeh Jahed, Hamed Heidari-Vala, Haleh Edalatkhah, Somaieh Kazemnejad
JOURNAL OF SURGICAL RESEARCH
(2020)
Review
Immunology
Ebrahim Mirzadegan, Hannaneh Golshahi, Somaieh Kazemnejad
INTERNATIONAL IMMUNOPHARMACOLOGY
(2020)
Review
Cell Biology
Mahmood Bozorgmehr, Shanti Gurung, Saeedeh Darzi, Shohreh Nikoo, Somaieh Kazemnejad, Amir-Hassan Zarnani, Caroline E. Gargett
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2020)
Article
Medicine, Research & Experimental
Reyhane Rahnama, Ahmad-Reza Mahmoudi, Somayeh Kazemnejad, Mansour Salehi, Ataollah Ghahiri, Haleh Soltanghoraee, Sedigheh Vafaei, Abbas Rezaei, Amir Hassan Zarnani
Summary: This study found for the first time that TPO is expressed at gene and protein levels in human reproductive organs, potentially explaining the higher frequency of abortion and infertility in patients with thyroid autoimmunity.
CLINICAL AND EXPERIMENTAL MEDICINE
(2021)
Article
Orthopedics
Arash Sharafat-Vaziri, Somayeh Khorasani, Maryam Darzi, Zahra Saffarian, Zahra Alizadeh, Mohammad Naghi Tahmasebi, Somaieh Kazemnejad
Article
Allergy
Mostafa Atashbasteh, Esmaeil Mortaz, Seyed Alireza Mahdaviani, Hamidreza Jamaati, Abdolamir Allameh
Summary: This study aimed to investigate changes in the expression of selected microRNAs in plasma exosomal fractions of severe asthma patients. The results showed significant differences in miRNA expression between asthma patients and controls, with miR-125b being up-regulated and miR-124, miR-133b, miR-130a down-regulated in patients. The up-regulation of miR-125b, along with its correlation with serum hs-CRP and IgE levels, suggests its potential role in inflammatory reactions and asthma phenotypes differentiation.
ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY
(2021)
Article
Veterinary Sciences
Abdolamir Allameh, Maryam Khanian, Mohammad-Amir Karimi-Torshizi, Ali Kalantari-Hesari
Summary: The study found that administering Lactobacillus plantarum 299v to broilers exposed to aflatoxins can mitigate hepatotoxicity. Broilers fed aflatoxins alone showed severe liver damage, including bile duct hyperplasia and necrosis. The improvement in growth performance and prevention of aflatoxin-related liver lesions can be mainly attributed to probiotic therapy.
Article
Immunology
Ebrahim Mirzadegan, Hannaneh Golshahi, Zahra Saffarian, Maryam Darzi, Somayeh Khorasani, Haleh Edalatkhah, Kioomars Saliminejad, Somaieh Kazemnejad
Summary: By combining MenSCs with a bilayer scaffold, wound healing in diabetic mice can be promoted. Immunofluorescence evaluation showed higher levels of CD31 and VWF, higher ratio of M2/M1 macrophages, greater expression of IVL, and higher levels of PGP9.5 in the bSC + MenSCs group.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Microbiology
Ata Garajei, Milad Parvin, Hady Mohammadi, Abdolamir Allameh, Azin Hamidavi, Masoud Sadeghi, Azadeh Emami, Serge Brand
Summary: This study evaluated the expression of miR-486-3p, miR-561-5p, miR-548-3p, and miR-509-5p in tissue biopsy samples with and without OSCC. The results showed that the expression of miR-486-3p and miR-561-5p was significantly lower in cancer samples compared to normal tissue samples, while miR-548-3p expression increased in the OSCC group compared to the control group. There was no significant difference in miR-509-5p expression between OSCC and control groups.
Article
Biochemistry & Molecular Biology
Bahareh Naeeni, Masoumeh Fakhr Taha, Mohamad Sajad Emami Aleagha, Abdolamir Allameh
Summary: The expression of Klotho protein changes significantly during the differentiation of mesenchymal stem cells into neuron-like cells. The increased expression of neuron-specific genes and accumulation of Klotho protein suggest the importance of Klotho in stem cell differentiation and response to cell death in the central nervous system.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Article
Pathology
Azin Hamidavi Asl, Mohammad Shirkhoda, Hana Saffar, Abdolamir Allameh
Summary: This study found that the H-ras gene mutation Gly12Ala (G6266A) is associated with recurrence in high-grade OSCC tumors.
HEAD & NECK PATHOLOGY
(2023)
Article
Medicine, General & Internal
Mohammad Reza Nazem, Mojgan Asadi, Maryam Adelipour, Niloofar Jabbari, Abdolamir Allameh
Summary: This study evaluated the effects of zinc supplementation on oxidative status and glycaemic parameters in overweight patients with type 2 diabetes mellitus (T2DM). The results showed that zinc supplementation for 8 weeks significantly improved the oxidative balance and controlled the glycaemic indices in these patients.
POSTGRADUATE MEDICAL JOURNAL
(2023)
Article
Obstetrics & Gynecology
Niloofar Motaref, Sheyda Jouhari, Afsaneh Mohammadzadeh, Somaieh Kazemnejad, Narges Madadi, Abolfazl Ghoodjani
INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE
(2020)
Article
Multidisciplinary Sciences
Abdolamir Allameh, Hamid Reza Ahmadi-Ashtiani, Narges Maleki
Article
Cell & Tissue Engineering
Simin Zafardoust, Somaieh Kazemnejad, Maryam Darzi, Mina Fathi-Kazerooni, Hilda Rastegari, Afsaneh Mohammadzadeh
STEM CELL REVIEWS AND REPORTS
(2020)