Article
Obstetrics & Gynecology
Yanli Hong, Jiayun Wu, Simin Yu, Miao Hui, Sipei Lin
Summary: The interaction between obesity and insulin resistance in polycystic ovary syndrome (PCOS) may be related to miRNA regulation through exosomes. Overexpression of miR-20b-5p and miR-106a-5p in serum exosomes can inhibit adipocyte differentiation and alleviate insulin resistance in PCOS mouse models.
REPRODUCTIVE SCIENCES
(2022)
Review
Cell Biology
Luiz F. Piochi, Ivo F. Machado, Carlos M. Palmeira, Anabela P. Rolo
Summary: Mitochondria play a crucial role in myogenesis and their interaction with myogenic differentiation needs further exploration. SESN2 may act as a regulator of mitochondrial quality control under stress conditions induced by ROS, suggesting its potential involvement in myogenic differentiation.
AGEING RESEARCH REVIEWS
(2021)
Article
Multidisciplinary Sciences
Zhengyu Qian, Jingwei Ye, Jinteng Li, Yunshu Che, Wenhui Yu, Peitao Xu, Jiajie Lin, Feng Ye, Xiaojun Xu, Zepeng Su, Dateng Li, Zhongyu Xie, Yanfeng Wu, Huiyong Shen
Summary: This study aimed to explore the role of crotonylation in myogenic differentiation and its potential as a target in myogenic dysfunction diseases. Through in vitro and in vivo experiments, it was confirmed that crotonylation affects muscle differentiation and the crotonylation eraser HDAC3 regulates myogenic differentiation. The findings contribute to a better understanding of the pathophysiological processes of muscle injury repair and sarcopenia.
JOURNAL OF ADVANCED RESEARCH
(2023)
Article
Oncology
Yanli Kang, Jianbin You, Yuhan Gan, Qianshun Chen, Chen Huang, Falin Chen, Xunyu Xu, Liangyuan Chen
Summary: This study found that hsa_circ_0001492, hsa_circ_0001439, and hsa_circ_0000896 were significantly upregulated in the serum and serum exosomes of patients with LUAD, suggesting that they may serve as effective biomarkers for the diagnosis of LUAD.
FRONTIERS IN ONCOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Chenyi Zhang, Lai Pan, Haizheng Zhang, Ting Ke, Yuxuan Yang, Lan Zhang, Lili Chen, Jingyi Tan
Summary: The study investigated the role of lncRNA MALAT1 and miRNA-124 in osteoclast differentiation, providing new clues for the pathogenesis of periodontitis.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Cell Biology
Yi Zhang, Ilimbek Beketaev, Yanlin Ma, Jun Wang
Summary: This study reveals that the activity of Phosphoglycerate mutase 2 is regulated by sumoylation, which is potentially involved in mutation-linked diseases. The K176R mutation in Phosphoglycerate mutase 2 impairs glycolysis and mitochondrial function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Aiwen Jiang, Hongyun Guo, Wangjun Wu, Honglin Liu
Summary: This study found that during myogenic differentiation, the level of autophagy was consistent with apoptosis, with increased apoptosis activating autophagy, which in turn inhibited apoptosis to prevent excessive cell death and maintain cell stability. The interaction between autophagy and apoptosis determines the balance of cell death and survival during skeletal muscle cell differentiation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Pinshi Ni, Luodan Yang, Fanghui Li
Summary: Exosomes are extracellular membrane vesicles containing RNA and proteins that play a crucial role in physiological and pathological processes. Skeletal muscle-derived exosomes (SkMCs-Exs) carry myokines, regulate receptor cells, and have effects on pathological states such as injury atrophy, aging, and vascular porosis. Exercise also regulates SkMCs-Exs and has physiological significance.
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Kento Mori, Hongkai Sun, Kazuki Miura, Siro Simizu
Summary: DPY19L3 is essential for myogenic differentiation, and its knockout affects cell differentiation, leading to suppression of MEK/ERK and p70S6K signaling pathways.
Article
Pathology
Josephine K. Dermawan, Sarah Chiang, Martee L. Hensley, William D. Tap, Cristina R. Antonescu
Summary: This study investigated the occurrence, clinicopathologic and genomic features of PDGFRB gene alterations in sarcomas with myogenic differentiation, and found recurrent hotspot mutations in a subset of patients. The most frequent mutations were identified in exon 12 (43%), exon 14 (N666K/S/T) (38%), and exon 18 (D850Y/H/ V or insertion/deletion) (19%). The study also revealed that PDGFRB-mutant sarcomas have a distinct genomic landscape compared to uterine and soft tissue leiomyosarcoma control groups, and are associated with aggressive clinical behavior and poor prognosis.
Article
Nanoscience & Nanotechnology
Tao Rui, Kai Wang, Aizhai Xiang, Jufeng Guo, Ning Tang, Xin Jin, Yimou Lin, Jian Liu, Xiaobing Zhang
Summary: In this study, piRNAs were identified as components of serum exosomes from HCC patients, with different expression compared to non-tumor donors. These piRNAs showed potential diagnostic value for HCC and could distinguish HCC patients from non-tumor donors, especially those with low tumor burden.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2023)
Article
Medicine, Research & Experimental
Yang Du, Rui Qiu, Lei Chen, Yuewen Chen, Zhifeng Zhong, Peng Li, Fangcheng Fan, Yong Cheng
Summary: This study investigated the potential molecular mechanism of remote ischemic preconditioning (RIPC)-mediated neuroprotection. By analyzing the differential metabolites and proteins in the serum exosomes of RIPC participants and control subjects, it was found that these differentially expressed substances were related to pathways associated with neurodegenerative diseases. The results suggest that serum exosomal metabolites can serve as promising biomarkers for RIPC and provide a rich dataset and framework for future analyses of cerebral ischemia-reperfusion injury under ischemia/reperfusion conditions.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Materials Science, Biomaterials
Ying Wang, Qingshuai Wang, Shengchang Luo, Zhoujiang Chen, Xiang Zheng, Ranjith Kumar Kankala, Aizheng Chen, Shibin Wang
Summary: The study demonstrates the fabrication of a conductive hydrogel scaffold using 3D bioprinting, incorporating PEDOT nanoparticles to enhance electrical conductivity and facilitate myogenic differentiation of C2C12 cells. The GelMA/PEDOT composite scaffolds showed favorable outcomes in terms of cell proliferation, migration, and differentiation, as confirmed by various in vitro investigations. Moreover, the effects of electrical signals on myogenic differentiation and myotube formation in vitro were also explored, showcasing the potential of conductive hydrogels for cell encapsulation and muscle tissue regeneration.
REGENERATIVE BIOMATERIALS
(2021)
Article
Multidisciplinary Sciences
P. Nayak, A. Colas, M. Mercola, S. Varghese, S. Subramaniam
Summary: This research compares the transcriptomic profiles of hiPSC lines of different myogenic specification levels, revealing temporal differences and the role of β-catenin transcriptional cofactors in mediating cellular interactions and external cues during differentiation.
Article
Biochemistry & Molecular Biology
Hui Song Cui, So Young Joo, Seung Yeol Lee, Yoon Soo Cho, Dong Hyun Kim, Cheong Hoon Seo
Summary: It is unclear how scar fibroblasts (SFs) affect keratinocytes in hypertrophic scars (HTS) through cell-cell interaction. This study investigated the effects of HTS-derived exosomes on the proliferation and differentiation of normal human keratinocytes (NHKs) and compared them with normal fibroblasts (NFs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Kaori Oka, Shusuke Fujioka, Yoshimi Kawamura, Yoshihiro Komohara, Takeshi Chujo, Koki Sekiguchi, Yuki Yamamura, Yuki Oiwa, Natsuko Omamiuda-Ishikawa, Shohei Komaki, Yoichi Sutoh, Satoko Sakurai, Kazuhito Tomizawa, Hidemasa Bono, Atsushi Shimizu, Kimi Araki, Takuya Yamamoto, Yasuhiro Yamada, Hiroyuki Oshiumi, Kyoko Miura
Summary: Naked mole-rats exhibit resistance to cancer through a dampened inflammatory response. The study found that immune cell infiltration in the skin of naked mole-rats was significantly lower compared to mice after carcinogenic insults. The mole-rats carry mutations in two necroptosis-related genes, which may contribute to their enhanced cancer resistance.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Emi Sano, Tatsuya Suzuki, Rina Hashimoto, Yumi Itoh, Ayaka Sakamoto, Yusuke Sakai, Akatsuki Saito, Daisuke Okuzaki, Daisuke Motooka, Yukiko Muramoto, Takeshi Noda, Tomohiko Takasaki, Jun-Ichi Sakuragi, Shohei Minami, Takeshi Kobayashi, Takuya Yamamoto, Yasufumi Matsumura, Miki Nagao, Toru Okamoto, Kazuo Takayama
Summary: Bronchial organoids (BO) and BO-derived air-liquid interface models (BO-ALI) can be used to model and study SARS-CoV-2 infection mechanisms and therapeutic methods. Ciliated cells are susceptible to virus infection, while basal cells are not. These models can also be used for airway regeneration studies.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Minas Nalbandian, Mingming Zhao, Hiroki Kato, Tatsuya Jonouchi, May Nakajima-Koyama, Takuya Yamamoto, Hidetoshi Sakurai
Summary: In this study, an atlas of hiPSC-MuPCs was established using single-cell RNA sequencing, revealing the heterogeneity of these cells and identifying distinct cell clusters. The study also found that noncycling progenitors have a higher regenerative capacity compared to committed cells. Furthermore, the E2F transcription factor family was identified as key regulators of hiPSC-MuPC proliferation.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biochemistry & Molecular Biology
Masahiro Nagano, Bo Hu, Shihori Yokobayashi, Akitoshi Yamamura, Fumiya Umemura, Mariel Coradin, Hiroshi Ohta, Yukihiro Yabuta, Yukiko Ishikura, Ikuhiro Okamoto, Hiroki Ikeda, Naofumi Kawahira, Yoshiaki Nosaka, Sakura Shimizu, Yoji Kojima, Ken Mizuta, Tomoko Kasahara, Yusuke Imoto, Killian Meehan, Roman Stocsits, Gordana Wutz, Yasuaki Hiraoka, Yasuhiro Murakawa, Takuya Yamamoto, Kikue Tachibana, Jan-Michel Peters, Leonid A. Mirny, Benjamin A. Garcia, Jacek Majewski, Mitinori Saitou
Summary: This study provides comprehensive insights into the nucleome analysis of mouse germ cell development in vitro, revealing the mechanisms of epigenetic reprogramming and euchromatization in spermatogonial development, and highlighting the importance of proper nucleome maturation for normal spermatogenesis.
Review
Oncology
Ryusaku Matsumoto, Hidetaka Suga, Hiroshi Arima, Takuya Yamamoto
Summary: The lack of human pituitary cell lines has limited the study of the molecular mechanisms of pituitary tumors. Recent research has successfully induced pituitary cells from human-induced pluripotent stem cells (hiPSCs), providing an opportunity to develop in vitro models of human pituitary adenomas. These models have great potential for discovering new drugs and investigating the development and pathophysiology of pituitary tumors.
Article
Biology
Yusuke Imoto, Tomonori Nakamura, Emerson G. Escolar, Michio Yoshiwaki, Yoji Kojima, Yukihiro Yabuta, Yoshitaka Katou, Takuya Yamamoto, Yasuaki Hiraoka, Mitinori Saitou
Summary: Single-cell RNA sequencing (scRNA-seq) is a powerful technique in biomedical research, but it is challenging to analyze the high-dimensional and noisy data it produces. In this study, a noise reduction method called RECODE is proposed to address these challenges and improve the analysis of scRNA-seq data. Results show that RECODE consistently resolves the statistical problem known as the curse of dimensionality and outperforms other methods in various analyses.
LIFE SCIENCE ALLIANCE
(2022)
Article
Cell Biology
Hiroyuki Nakajima, Hiroyuki Ishikawa, Takuya Yamamoto, Ayano Chiba, Hajime Fukui, Keisuke Sako, Moe Fukumoto, Kenny Mattonet, Hyouk-Bum Kwon, Subhra P. Hui, Gergana D. Dobreva, Kazu Kikuchi, Christian S. M. Helker, Didier Y. R. Stainier, Naoki Mochizuki
Summary: By using live imaging-based lineage tracing and single-cell transcriptomics in zebrafish embryos, researchers have identified the origin of venous endothelial cells (ECs) that make up the majority of the hematopoietic stem and progenitor cell (HSPC) niche. They found that cells expressing the gene islet1 (isl1), surprisingly derived from the endoderm, differentiate into ECs and contribute to the HSPC niche. This study demonstrates that the endothelial specialization in the HSPC niche is determined, in part, by the origin of the ECs.
DEVELOPMENTAL CELL
(2023)
Article
Multidisciplinary Sciences
Yoshihiro Yamanaka, Sofiane Hamidi, Kumiko Yoshioka-Kobayashi, Sirajam Munira, Kazunori Sunadome, Yi Zhang, Yuzuru Kurokawa, Rolf Ericsson, Ai Mieda, Jamie L. Thompson, Janet Kerwin, Steven Lisgo, Takuya Yamamoto, Naomi Moris, Alfonso Martinez-Arias, Taro Tsujimura, Cantas Alev
Summary: A pluripotent stem cell-derived 3D model called "axioloid" has been developed to accurately capture the dynamics of segmentation and somitogenesis in humans. This model shows similarities to the human embryo and can be used to study congenital spine diseases.
Article
Medicine, General & Internal
Keiko Imamura, Yuishin Izumi, Makiko Nagai, Kazutoshi Nishiyama, Yasuhiro Watanabe, Ritsuko Hanajima, Naohiro Egawa, Takashi Ayaki, Ryosuke Oki, Koji Fujita, Ryuji Uozumi, Akiko Morinaga, Tomoko Hirohashi, Yosuke Fujii, Takuya Yamamoto, Harutsugu Tatebe, Takahiko Tokuda, Naoto Takahashi, Satoshi Morita, Ryosuke Takahashi, Haruhisa Inoue
Summary: This study aimed to evaluate the safety and efficacy of bosutinib in patients with ALS, finding good tolerability in patients with a dose limit of 300mg QD, with a subset of patients showing treatment response to bosutinib, and no ALS-specific adverse events were observed.
Article
Biochemistry & Molecular Biology
Sayuri Gyobu-Motani, Yukihiro Yabuta, Ken Mizuta, Yoshitaka Katou, Ikuhiro Okamoto, Masanori Kawasaki, Ayaka Kitamura, Tomoyuki Tsukiyama, Chizuru Iwatani, Hideaki Tsuchiya, Taro Tsujimura, Takuya Yamamoto, Tomonori Nakamura, Mitinori Saitou
Summary: Human in vitro oogenesis is better understood through studying in vitro oogenesis in cynomolgus monkeys. In this model, epigenetic reprogramming, including inactivation of the Xi and activation of the Xa, occurs during differentiation of embryonic stem cells. However, Xa and Xi remain epigenetically asymmetric. This in vitro model closely resembles the human system and can be used to advance research on human in vitro oogenesis.
Article
Medicine, Research & Experimental
Rina Hashimoto, Tomokazu Tamura, Yukio Watanabe, Ayaka Sakamoto, Naoko Yasuhara, Hayato Ito, Masahiro Nakano, Hiromitsu Fuse, Akira Ohta, Takeshi Noda, Yasufumi Matsumura, Miki Nagao, Takuya Yamamoto, Kazuo Takayama, Takasuke Fukuhara
Summary: To combat a broad range of coronaviruses, it is important to develop drugs that target viral proteins as well as host proteins/cellular processes. Autophagy has been found to play a significant role in coronavirus infection. In this study, autophagic modulators were screened using airway organoids to identify compounds that could potentially fight against infections by various coronaviruses. Among the tested compounds, cycloheximide and thapsigargin showed dose-dependent reduction in SARS-CoV-2 infection efficiency. Cycloheximide treatment not only reduced the infection efficiency of SARS-CoV-2 variants but also other human coronaviruses. However, the use of cycloheximide may have side effects on host cells, such as inhibiting protein synthesis.
MOLECULAR PHARMACEUTICS
(2023)
Article
Cell & Tissue Engineering
Takashi Shinohara, Takuya Yamamoto, Hiroko Morimoto, Yusuke Shiromoto, Mito Kanatsu-Shinohara
Summary: This study found that spermatogonial stem cells (SSCs) can induce immune tolerance by enhancing the expression of immunosuppressive molecule PD-L1, leading to the successful birth of allogeneic offspring. It was also found that activation of the MAPK14-BCL6B pathway can induce PD-L1 expression and promote self-renewal of SSCs.
Article
Urology & Nephrology
Takahisa Yoshikawa, Akiko Oguchi, Naoya Toriu, Yuki Sato, Takashi Kobayashi, Osamu Ogawa, Hironori Haga, Satoko Sakurai, Takuya Yamamoto, Yasuhiro Murakawa, Motoko Yanagita
Summary: Ectopic lymphoid structures called tertiary lymphoid tissues (TLTs) develop in kidney diseases and worsen renal prognosis. Single-nucleus RNA sequencing and validation experiments reveal that TLTs amplify inflammation in injured kidneys by promoting proinflammatory phenotypes of surrounding cells. These findings shed light on TLT pathology and suggest potential therapeutic targets.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Biology
Satoshi Kozuki, Mio Kabata, Satoko Sakurai, Keiko Iwaisako, Tomomi Nishimura, Masakazu Toi, Takuya Yamamoto, Fumiko Toyoshima
Summary: This study reveals that hepatocyte proliferation in different liver lobular zones is regulated in a spatiotemporal manner during pregnancy in mice. The periportal hepatocyte proliferation in midgestation is critical for maternal glycogen metabolism and fetal size.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Sayaka Deguchi, Kaori Kosugi, Rina Hashimoto, Ayaka Sakamoto, Masaki Yamamoto, Rafal P. Krol, Peter Gee, Ryosuke Negoro, Takeshi Noda, Takuya Yamamoto, Yu-suke Torisawa, Miki Nagao, Kazuo Takayama
Summary: This study investigates the liver pathophysiology in COVID-19 patients and demonstrates that SARS-CoV-2 infection induces hepatic dysfunctions. The combination of anti-viral and immunosuppressive drugs is found to effectively treat hepatic dysfunctions caused by SARS-CoV-2 infection.
