Article
Biochemistry & Molecular Biology
Tomasz Boruta, Anna Scigaczewska
Summary: This study evaluated the interaction of different microorganisms on oxytetracycline production and morphological development using co-culture methods. The co-culture of Streptomyces rimosus with Streptomyces noursei showed an enhancement in antibiotic production, while the presence of Mucor racemosus resulted in a delay in production. Different initiation strategies for the co-culture were tested, with improved oxytetracycline titers when S. noursei was co-inoculated with S. rimosus as spores.
Article
Plant Sciences
Han Wang, Huan Qi, Shao-Yong Zhang, Wen-Shuai Song, Li-Qin Zhang, Wen-Sheng Xiang, Ji-Dong Wang
Summary: A chemical investigation of Streptomyces sp. Hu186 led to the discovery of known quinone antibiotics and new metabolites with unique structures, which showed moderate cytotoxic activity against tumor cell lines.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Microbiology
Matthieu Nicault, Ali Zaiter, Stephane Dumarcay, Patrick Chaimbault, Eric Gelhaye, Pierre Leblond, Cyril Bontemps
Summary: The study explored the impact of co-culture between Streptomyces and fungi on the production of bioactive compounds, finding that co-culture had a significant effect on the metabolic expression of each partner and facilitated the expression of specific compounds.
Article
Biotechnology & Applied Microbiology
Tomasz Boruta, Anna Scigaczewska, Marcin Bizukojc
Summary: This study aimed to characterize the bioprocess kinetics and secondary metabolites production in a novel microbial co-cultivation system involving Streptomyces noursei and Aspergillus terreus. The co-cultures showed concurrent development and observable biosynthetic activity of both species. The study found that the co-cultures were effective in enhancing the biosynthesis of several metabolic products.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Evan M. F. Shepherdson, Marie A. Elliot
Summary: Streptomyces bacteria have complex life cycles and remarkable metabolic capabilities. Their recently discovered exploration ability allows them to successfully compete with other microbes. Nutrient availability affects exploration rates and metabolic output, with glycerol dramatically increasing exploration rates. Different siderophores play different roles in exploration, and the alternative siderophore foroxymithine is more important than desferrioxamine during coculture.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Anton P. Tyurin, Vera A. Alferova, Alexander S. Paramonov, Maxim V. Shuvalov, Gulnara K. Kudryakova, Eugene A. Rogozhin, Alexander Y. Zherebker, Vladimir A. Brylev, Alexey A. Chistov, Anna A. Baranova, Mikhail V. Biryukov, Igor A. Ivanov, Igor A. Prokhorenko, Natalia E. Grammatikova, Tatyana V. Kravchenko, Elena B. Isakova, Elena P. Mirchink, Elena G. Gladkikh, Elena V. Svirshchevskaya, Andrey V. Mardanov, Aleksey V. Beletsky, Milita V. Kocharovskaya, Valeriya V. Kulyaeva, Alexander S. Shashkov, Dmitry E. Tsvetkov, Nikolay E. Nifantiev, Alexander S. Apt, Konstantin B. Majorov, Svetlana S. Efimova, Nikolai V. Ravin, Evgeny N. Nikolaev, Olga S. Ostroumova, Genrikh S. Katrukha, Olda A. Lapchinskaya, Olga A. Dontsova, Stanislav S. Terekhov, Ilya A. Osterman, Zakhar O. Shenkarev, Vladimir A. Korshun
Summary: A novel family of natural lipoglycopeptides produced by Streptomyces sp. INA-Ac-5812, including gausemycins A and B, have been reported. These compounds exhibit pronounced activity against Gram-positive bacteria and show significant differences in mechanism compared to known glyco- and lipopeptides.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Microbiology
Evellin do Espirito Santo, Marina Ishii, Uelinton Manoel Pinto, Marcelo Chuei Matsudo, Joao Carlos Monteiro De Carvalho
Summary: The applications of microalgae biomass have been widely studied worldwide. Bacterial interactions with microalgae and cyanobacteria can positively impact their production process to ensure safety and profitability. This interaction promotes improvements in biomolecule production and the development of innovative products such as prebiotics and antimicrobial compounds. Microalgae can be utilized in pharmaceutical, cosmetic, and food bioproducts to align with the Sustainable Development Goals.
Review
Biotechnology & Applied Microbiology
James Mason, Daniel Ohlund
Summary: The tumor microenvironment plays a crucial role in cancer initiation and progression. Understanding the interactions between cancer cells and the various components of the tumor microenvironment is essential for developing effective treatment strategies. Co-culture models have been developed to study these interactions, providing insights into the role of the stroma and tumor-stromal interactions.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Min Beom Kim, Soonho Hwangbo, Sungho Jang, Yun Kee Jo
Summary: The recent increase in complex physiological host-microbe interactions has led to a demand for in vitro models that replicate the microbial microenvironment in the human body. Organoids, an in vitro culture system that closely mimics the structure, function, and genetic features of human organs, are emerging as a promising tool for studying host-microbe interactions. Co-cultures of organoids with microbes show great potential in simulating complex host-microbe interactions and advancing related fields. This review provides an overview of bioengineering approaches for microbe-co-cultured organoids and highlights the latest developments in their applications for studying human physiology and pathophysiology.
MATERIALS TODAY BIO
(2022)
Article
Plant Sciences
Zhiyan Wang, Feng-Xian Yang, Chongxi Liu, Li Wang, Yuxin Qi, Minghang Cao, Xiaowei Guo, Jie Li, Xueshuang Huang, Jing Yang, Sheng-Xiong Huang
Summary: Nexphenazine A, a phenazine-polyketide hybrid compound, was isolated from Streptomyces sp. KIB-H483. The biosynthesis of nexphenazine involves a gene cluster, with npzI gene playing a key role.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Agricultural Engineering
Mohamad Padri, Nittaya Boontian, Neung Teaumroong, Pongdet Piromyou, Chatlada Piasai
Summary: This study shows that the co-culture of a microalga and bacterium can effectively treat wastewater and enhance biodiesel production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Infectious Diseases
Balasubramanian Cibichakravarthy, Polapass Arul Jose
Summary: Streptomyces are a rich source of structurally diverse microbial natural products, with the potential to produce numerous novel secondary metabolites. However, there is currently a mismatch between the speed of genome analysis and the discovery of new compounds.
Article
Engineering, Chemical
Bibek Raj Bhattarai, Karan Khadayat, Niraj Aryal, Babita Aryal, Uttam Lamichhane, Keshab Bhattarai, Nabin Rana, Bishnu P. Regmi, Achyut Adhikari, Sandeep Thapa, Niranjan Parajuli
Summary: This study characterized actinomycetes from Nepal and predicted their secondary metabolites using various analysis methods. The BN14 isolate showed broad-spectrum antibacterial activity and may be a potential source for discovering new antimicrobial agents in the future.
Review
Biotechnology & Applied Microbiology
Tomasz Boruta
Summary: Filamentous microorganisms are potent sources of bioactive secondary metabolites formed in response to complex environmental signals. Despite the extensively reviewed biosynthetic outcomes of co-cultures, the bioprocess aspects are often overlooked. This review discusses submerged co-cultivation strategies for triggering and enhancing secondary metabolites production in Streptomyces, focusing on co-culture initiation, experimental setup, controls, and influencing outcomes.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tomasz Boruta, Anna Scigaczewska, Marcin Bizukojc
Summary: Microbial co-cultivation is commonly used to induce secondary metabolic pathways and discover novel molecules. This study focused on the co-cultivation of Aspergillus terreus and Streptomyces rimosus in a bioreactor, revealing the production of over 40 secondary metabolites, including unique molecules observed only under co-cultivation conditions. Despite the dominance of Streptomyces rimosus, Aspergillus terreus still had an observable influence on secondary metabolite production and substrate utilization in co-culture. Monitoring dissolved oxygen levels was identified as a fast method to determine the dominant microorganism during co-cultivation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Diego Barriales, Itziar Martin-Ruiz, Ana Carreras-Gonzalez, Marta Montesinos-Robledo, Mikel Azkargorta, Ibon Iloro, Iraide Escobes, Teresa Martin-Mateos, Estibaliz Atondo, Ainhoa Palacios, Monika Gonzalez-Lopez, Laura Barcena, Ana R. Cortazar, Diana Cabrera, Ainize Pena-Cearra, Sebastiaan M. van Liempd, Juan M. Falcon-Perez, Miguel A. Pascual-Itoiz, Juana Maria Flores, Leticia Abecia, Aize Pellon, Maria Luz Martinez-Chantar, Ana M. Aransay, Alberto Pascual, Felix Elortza, Edurne Berra, Jose Luis Lavin, Hector Rodriguez, Juan Anguita
Summary: Lyme carditis is associated with down-regulation of mitochondrial components in the heart and long-term memory-like responses in macrophages exposed to B. burgdorferi. This memory-like response can be manipulated in vivo by inhibiting glycolysis, leading to reduced inflammation and spirochetal burdens, and the recovery of tissue mitochondrial components.
Article
Biotechnology & Applied Microbiology
Jose Miguel Mancheno, Estibaliz Atondo, Julen Tomas-Cortazar, Jose Luis Lavin, Laura Plaza-Vinuesa, Itziar Martin-Ruiz, Diego Barriales, Ainhoa Palacios, Claudio Daniel Navo, Leticia Sampedro, Ainize Pena-Cearra, Miguel Angel Pascual-Itoiz, Janire Castelo, Ana Carreras-Gonzalez, Donatello Castellana, Aize Pellon, Susana Delgado, Patricia Ruas-Madiedo, Blanca delas Rivas, Leticia Abecia, Rosario Munoz, Gonzalo Jimenez-Oses, Juan Anguita, Hector Rodriguez
Summary: The occurrence and development of colorectal cancer is associated with the presence of Fusobacterium nucleatum, dietary components, and reduction of acetylated derivatives of spermidine. The study found that a new tannase orthologue of F. nucleatum (TanBF(nn)) has structural differences affecting the active site and substrate accessibility. Spermidine and its derivatives inhibit the hydrolytic activity of TanBF(nn) and increase the toxicity of gallotannins to F. nucleatum.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Infectious Diseases
Ramon Santamaria, Ana Martinez-Carrasco, Jesus Martin, Jose R. Tormo, Ignacio Perez-Victoria, Ignacio Gonzalez, Olga Genilloud, Fernando Reyes, Margarita Diaz
Summary: Xylem sap from grapevines has a positive effect on antibiotic production in Streptomyces bacteria, inducing the production of known compounds and new unidentified compounds. The active fraction of the sap contains small molecules that act as elicitors, but there may be other unidentified elicitors involved.
Article
Gastroenterology & Hepatology
Maria Juarez-Fernandez, Naroa Goikoetxea-Usandizaga, David Porras, Maria Victoria Garcia-Mediavilla, Miren Bravo, Marina Serrano-Macia, Jorge Simon, Teresa C. Delgado, Sofia Lachiondo-Ortega, Susana Martinez-Florez, Oscar Lorenzo, Mercedes Rincon, Marta Varela-Rey, Leticia Abecia, Hector Rodriguez, Juan Anguita, Esther Nistal, Maria Luz Martinez-Chantar, Sonia Sanchez-Campos
Summary: This study provides evidence for the relevance of the interaction between mitochondria and microbiota in NASH and identifies targeting this interaction as a valuable therapeutic approach.
Review
Biochemistry & Molecular Biology
Ricardo Sanchez de la Nieta, Ramon Santamaria, Margarita Diaz
Summary: Bacteria of the Streptomyces genus are a valuable biotech resource due to their ability to produce compounds and enzymes of interest in various fields. Understanding their physiology and regulatory mechanisms is crucial for their manipulation and application. Two-component systems (TCSs) are the main signal transduction mechanism in prokaryotes, detecting stimuli and triggering cellular responses for adaptation. This review focuses on the TCSs described in Streptomyces coelicolor, an important model species. Unraveling the complex regulatory network formed by TCSs is essential for their biotechnological potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Michelle J. Klouwens, Jos J. A. Trentelman, Diego Barriales, Jasmin I. Ersoz, Mikel Azkargorta, Felix Elortza, Radek Sima, Ondrej Hajdusek, Julen Tomas Cortazar, Iraide Escobes Corcuera, Emil Colstrup, Abhijeet Nayak, Itziar Martin Ruiz, Hector Rodriguez, Ard M. Nijhof, Juan Anguita, Joppe W. R. Hovius
Summary: In this study, differential protein production in the salivary glands of Ixodes ricinus ticks in response to Borrelia afzelii infection and different feeding conditions was identified using quantitative proteomics. Recombinant tick proteins selected from this study significantly reduced the post-engorgement weights of I. ricinus nymphs in experimental animal models, but efficient transmission of B. afzelii to the murine host was still observed.
Article
Biochemistry & Molecular Biology
Ramon I. Santamaria, Ana Martinez-Carrasco, Jose R. Tormo, Jesus Martin, Olga Genilloud, Fernando Reyes, Margarita Diaz
Summary: The co-culturing of microorganisms is a well-known strategy to study microbial interactions in the laboratory. In this study, the effects of the interaction between nine Streptomyces species and predator bacteria Myxococcus xanthus were investigated. It was found that certain Streptomyces species induce mound formation of M. xanthus on complex media. Moreover, the study identified siderophores produced by S. griseus and molecules produced by S. peucetius that affect the behavior of M. xanthus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Ainize Pena-Cearra, Deguang Song, Janire Castelo, Ainhoa Palacios, Jose Luis Lavin, Mikel Azkargorta, Felix Elortza, Miguel Fuertes, Miguel Angel Pascual-Itoiz, Diego Barriales, Itziar Martin-Ruiz, Asier Fullaondo, Ana M. Aransay, Hector Rodriguez, Noah W. Palm, Juan Anguita, Leticia Abecia
Summary: There is potential association between mitochondrial dysfunction and inflammatory bowel diseases (IBD). The bidirectional interaction between the intestinal microbiota and host mitochondria may affect intestinal inflammation. The study shows that fecal microbiota transplantation (FMT) from MCJ-deficient mice to germ-free mice increases susceptibility to colitis. Furthermore, the study identifies potential microbial signatures associated with complex I deficiency and disease progression.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Gastroenterology & Hepatology
Ainize Pena-Cearra, Janire Castelo, Jose Luis Lavin, Monika Gonzalez-Lopez, Miguel Angel Pascual-Itoiz, Miguel Fuertes, Virginia Gutierrez de Juan, Laura Barcena, Itziar Martin-Ruiz, Aize Pellon, Iratxe Seoane, Diego Barriales, Ainhoa Palacios, Asier Fullaondo, Iago Rodriguez-Lago, Maria L. Martinez-Chantar, Ana M. Aransay, Hector Rodriguez, Juan Anguita, Leticia Abecia
Summary: Anti-TNF therapy is effective in inducing and maintaining remission in intestinal bowel disease. However, a certain percentage of patients do not respond to this therapy. This study suggests that the absence of MCJ, a natural inhibitor of respiratory chain Complex I, leads to changes in gut microbiota, which is a critical determinant of the lack of response in a mouse model of inflammation. The study also highlights the role of macrophage mitochondrial function in the gut ecological niche in affecting the severity of inflammation and the response to current therapies.
Article
Multidisciplinary Sciences
Ainize Pena-Cearra, Miguel Angel Pascual-Itoiz, Jose Luis Lavin, Miguel Fuertes, Itziar Martin-Ruiz, Janire Castelo, Ainhoa Palacios, Diego Barriales, Asier Fullaondo, Ana M. Aransay, Hector Rodriguez, Juan Anguita, Leticia Abecia
Summary: Inflammatory bowel disease (IBD) is a complex and chronic disease. This study found that MCJ deficiency leads to increased TACE activity in chronic inflammation, affecting the metabolic adaptation of colon tissue and potentially associated with reduced microbial members associated with inflammation.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Carolina Simo, Tiziana Fornari, Monica R. Garcia-Risco, Ainize Pena-Cearra, Leticia Abecia, Juan Anguita, Hector Rodriguez, Virginia Garcia-Canas
Summary: In this study, a novel screening strategy was developed to evaluate the effects of food and natural extract on Klebsiella pneumoniae's TMA production. Oregano and red thyme extracts were found to have the potential to inhibit TMA formation.
Article
Microbiology
Ainize Pena-Cearra, Alejandro Belanche, Monika Gonzalez-Lopez, Jose Luis Lavin, Miguel Angel Pascual-Itoiz, Elisabeth Jimenez, Hector Rodriguez, Ana Ma Aransay, Juan Anguita, David R. Yanez-Ruiz, Leticia Abecia
Summary: The study assessed changes in the circulating microbiome by sequencing PBMC bacterial DNA from goats with or without rumen liquid transplantation in early life. The PBMC microbiome composition differed from the colon microbiota, with Proteobacteria as the predominant phylum. The study suggests that PBMC microbiome in healthy ruminants could play a role in homeostatic conditions.
Article
Gastroenterology & Hepatology
Aize Pellon, Diego Barriales, Ainize Pena-Cearra, Janire Castelo-Careaga, Ainhoa Palacios, Nerea Lopez, Estibaliz Atondo, Miguel Angel Pascual-Itoiz, Itziar Martin-Ruiz, Leticia Sampedro, Monika Gonzalez-Lopez, Laura Barcena, Teresa Martin-Mateos, Jose Maria Landete, Rafael Prados-Rosales, Laura Plaza-Vinuesa, Rosario Munoz, Blanca de las Rivas, Juan Miguel Rodriguez, Edurne Berra, Ana M. Aransay, Leticia Abecia, Jose Luis Lavin, Hector Rodriguez, Juan Anguita
Summary: The study demonstrates that innate immune memory plays a crucial role in promoting anti-inflammatory homeostasis by modulating bacterial intracellular survival and reducing the release of pro-inflammatory signals. Changes in metabolic pathways discovered in memory cells contribute to maintaining gut homeostasis.