Article
Microbiology
Caiquan Zhao, Jie Gao, Yongzhi Wang, Lina Ji, Hui Qin, Wei Hu, Yang Yang
Summary: This study describes a novel recombinant bovine herpes virus type I expressing rabies virus glycoprotein, which induced a protective immune response in mice and cattle.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Edward Trybala, Nadia Peerboom, Beata Adamiak, Malgorzata Krzyzowska, Jan-Ake Liljeqvist, Marta Bally, Tomas Bergstrom
Summary: The study shows that the HSV-2 mutant deficient in mgG expression has a defect in releasing progeny virions from infected cells. The mgG of HSV-2 plays a critical role in balancing virus interactions with GAG chains, preventing trapping of progeny virions on infected cell surfaces. This balance is necessary to ensure proper release of virions.
Review
Pharmacology & Pharmacy
Yiwei Huang, Yuyun Song, Jichen Li, Changning Lv, Zhe-Sheng Chen, Zhijun Liu
Summary: Human herpes simplex viruses use cell surface receptors such as heparan sulfate, herpesvirus entry mediator, and nectin-1/-2, 3-O-sulfated heparan sulfate to enter host cells. No vaccine or curative treatment is currently available for HSV infections.
DRUG DISCOVERY TODAY
(2022)
Article
Pharmacology & Pharmacy
Rui Tian, Fei Ju, Mengqin Yu, Zhiqi Liang, Zilong Xu, Min Zhao, Yaning Qin, Yanhua Lin, Xiaoxuan Huang, Yating Chang, Shaopeng Li, Wenfeng Ren, Chaolong Lin, Ningshao Xia, Chenghao Huang
Summary: Infections caused by herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) are a serious global health issue. The discovery and development of virus-neutralizing monoclonal antibodies (NAbs) could be a potential approach to treat or prevent HSV infection. In this study, five HSV NAbs were isolated from mice immunized with both HSV-1 and HSV-2, and two of them exhibited strong neutralizing activity against both HSV-1 and HSV-2.
ANTIVIRAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Xiao Zhang, Junping Hong, Ling Zhong, Qian Wu, Shanshan Zhang, Qianying Zhu, Haiwen Chen, Dongmei Wei, Rui Li, Wanlin Zhang, Xinyu Zhang, Guosong Wang, Xiang Zhou, Junyu Chen, Yinfeng Kang, Zhenghui Zha, Xiaobing Duan, Yang Huang, Cong Sun, Xiangwei Kong, Yan Zhou, Yanhong Chen, Xiaoping Ye, Qisheng Feng, Shaowei Li, Tong Xiang, Song Gao, Mu-Sheng Zeng, Qingbing Zheng, Yixin Chen, Yi-Xin Zeng, Ningshao Xia, Miao Xu
Summary: This study identified two neutralizing antibodies that effectively neutralize EBV infection and provide protection against EBV-induced lymphoproliferative disorders in humanized mice. The antibodies inhibit membrane fusion through interference with gB-cell interaction and activation, offering potential targets for antiviral therapies and vaccines.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Bishajit Sarkar, Md. Asad Ullah, Yusha Araf, Sowmen Das, Md. Hasanur Rahman, Abu Tayab Moin
Summary: HSV is a highly infectious virus with two types, HSV-1 and HSV-2, infecting millions worldwide. This study designed three polyvalent subunit vaccines targeting multiple strains of HSV, which showed stability and potential effectiveness through molecular docking and simulation studies. Validation of these vaccines in wet lab-based studies is still needed.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Chemistry, Applied
Mohamed M. Mohyeldin, Ahmaida F. S. Tarbah, Nehal M. El-Deeb, Nabila M. Ghazy, Amr El-Hawiet
Summary: This study provides the first comparative analysis of the anti-HSV1 activity and phytochemical components of Artemisia herba-alba and Thymus capitatus collected from Egypt and Libya. The results demonstrate that the ethanolic extracts of Egyptian Thymus capitatus exhibit the most potent anti-viral activity.
NATURAL PRODUCT RESEARCH
(2022)
Article
Immunology
Yang Wang, Runyang Wang, Han Hu, Jing Jin, Linkang Cai, Siqi Zhang, Fan Yi, Yanxia Li, Zhiqiang Zheng, Qin Zhou, Zhizheng Fang, Binlei Liu
Summary: Oncolytic virotherapy is a safe and emerging therapeutic approach, and a new type of oncolytic herpes simplex virus armed with Bispecific Antibody has been developed for treating human malignancies. Preclinical safety evaluation has shown that this therapy is safe and suitable for clinical trials. It has obtained FDA approval for Phase I clinical trials in the United States.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Andrew G. Diamos, Mary D. Pardhe, Melissa H. Bergeman, Aigerim S. Kamzina, Michelle P. DiPalma, Sara Aman, Artemio Chaves, Kenneth Lowe, Jacquelyn Kilbourne, Ian B. Hogue, Hugh S. Mason
Summary: This study designed a self-binding recombinant immune complex (RIC) vaccine that mimics the larger immune complexes (ICs) generated during natural infection. The results showed that RIC formed larger complexes and enhanced C1q receptor binding 25-fold compared to traditional IC. Immunization with RIC induced significantly higher gD-specific antibody titers and stronger virus-specific neutralization against HSV-2.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Mette Ratzer Freytag, Sofie Eg Jorgensen, Michelle Molgaard Thomsen, Ali Al-Mousawi, Alon Schneider Hait, David Olagnier, Jakob T. Bay, Marie Helleberg, Trine H. Mogensen
Summary: This study describes a 19-year-old woman with systemic HSV-1 infection and HLH, as well as a fatal course of neonatal herpesvirus infection postpartum. Investigation showed impaired antiviral responses in the mother's cells, potentially caused by variants in CASP8 or other noncoding regions of the genome.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Jie Wang, Kun-Te Shang, Qiong-Hong Ma, Zhao-Ying Dong, Yi-Hong Chen, Yu-Feng Yao
Summary: This study aimed to determine whether HSV-1 can be transmitted through TNTs and to investigate the effect of inhibiting the Arp2/3 complex on the intercellular transmission of HSV-1. The results showed that HSV-1 can be transmitted through TNTs and that inhibiting the Arp2/3 complex reduces the number of TNTs and the spread of HSV-1. This finding provides new insights into the transmission mode of HSV-1 and suggests a potential new antiviral target.
Article
Microbiology
Ye Liu, Qiao You, Fang Zhang, Deyan Chen, Zhenping Huang, Zhiwei Wu
Summary: Harringtonine (HT) significantly inhibited HSV-1 infection and two ACV-resistant strains by mainly targeting HVEM and reducing the early stage of HSV-1 infection. The study demonstrated that HT could be a promising therapeutic candidate for mitigating HSV-1-induced pathogenesis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jing Jin, Runyang Wang, Junhan Yang, Han Hu, Di Wang, Linkang Cai, Zhizheng Fang, Shuang Dong, Sheng Hu, Yang Wang, Binlei Liu
Summary: In this study, we generated oHSV2-BsAb to arm oncolytic viruses and found that they showed improved oncolytic potency. Our modified oncolytic viruses not only induced oncolysis in tumor cells, but also activated peripheral blood mononuclear cells to kill tumor cells. Additionally, the oncolytic viruses upregulated PD-L1 expression on tumor cells, enhancing the targeting effect of BsAb molecules and the oncolytic effect.
HUMAN GENE THERAPY
(2022)
Article
Immunology
Xiaoqian Tang, Jing Cao, Jialin Zhang, Jing Xing, Xiuzhen Sheng, Wenbin Zhan
Summary: In this study, monoclonal antibodies (mAbs) specific to the glycoprotein (G) of Hirame rhabdovirus (HIRRV) were successfully produced, showing virus-neutralizing activity in vitro and in vivo. These findings have the potential to serve as effective detection probes and potential neutralizing antibodies for HIRRV.
MICROBIAL PATHOGENESIS
(2021)
Article
Microbiology
Ilona Rissanen, Stefanie A. Krumm, Robert Stass, Annalis Whitaker, James E. Voss, Emily A. Bruce, Sylvia Rothenberger, Stefan Kunz, Dennis R. Burton, Juha T. Huiskonen, Jason W. Botten, Thomas A. Bowden, Katie J. Doores
Summary: Research demonstrates that the Gn glycoprotein of HTNV can elicit a neutralizing antibody response in rabbits, leading to the isolation of neutralizing and non-neutralizing monoclonal antibodies. Molecular-level insights on how the Gn glycoprotein may be targeted by the antibody-mediated immune response are provided for the first time. These findings may inform rational vaccine design approaches focused on targeting the hantavirus glycoprotein envelope.
Article
Virology
Kinga Grabowska, Magda Wachalska, Malgorzata Graul, Michal Rychlowski, Krystyna Bienkowska-Szewczyk, Andrea D. Lipinska
Article
Infectious Diseases
Erik Alm, Eeva K. Broberg, Thomas Connor, Emma B. Hodcroft, Andrey B. Komissarov, Sebastian Maurer-Stroh, Angeliki Melidou, Richard A. Neher, Aine O'Toole, Dmitriy Pereyaslov
Article
Virology
Edward Trybala, Nadia Peerboom, Beata Adamiak, Malgorzata Krzyzowska, Jan-Ake Liljeqvist, Marta Bally, Tomas Bergstrom
Summary: The study shows that the HSV-2 mutant deficient in mgG expression has a defect in releasing progeny virions from infected cells. The mgG of HSV-2 plays a critical role in balancing virus interactions with GAG chains, preventing trapping of progeny virions on infected cell surfaces. This balance is necessary to ensure proper release of virions.
Article
Surgery
John M. Softeland, Magnus Gisslen, Jan-Ake Liljeqvist, Vanda Friman, Emily de Coursey, Kristjan Karason, Jan Ekelund, Marie Felldin, Jesper Magnusson, Seema Baid-Agrawal, Carin Wallquist, Andreas Schult, Hanna Jacobsson, Anders Bergdahl, Mats Bemark, Lars-Magnus Andersson, Inger Holm Gunnarsson, Jan Stenstrom, Susannah Leach
Summary: Solid organ transplant recipients (SOTRs) exhibit comparable S-specific antibody responses to SARS-CoV-2 infection after COVID-19 as immunocompetent controls, but differ in N-specific antibody responses.
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Infectious Diseases
Lukasz Rabalski, Maciej Kosinski, Natalia Mazur-Panasiuk, Boguslaw Szewczyk, Krystyna Bienkowska-Szewczyk, Ravi Kant, Tarja Sironen, Krzysztof Pyrc, Maciej Grzybek
Summary: This study analyzed the possible zoonotic spill-over of SARS-CoV-2 and reported the transmission of mink-adapted SARS-CoV-2 from farmed mink to humans for at least 3 months.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Natalia Mazur-Panasiuk, Lukasz Rabalski, Tomasz Gromowski, Grzegorz Nowicki, Michal Kowalski, Witold Wydmanski, Piotr Szulc, Maciej Kosinski, Karolina Gackowska, Natalia Drweska-Matelska, Jakub Grabowski, Anna Piotrowska-Mietelska, Boguslaw Szewczyk, Krystyna Bienkowska-Szewczyk, Jakub Swadzba, Pawel Labaj, Maciej Grzybek, Krzysztof Pyrc
Summary: Routine genomic surveillance of COVID-19 samples collected in Poland during summer 2021 identified a SARS-CoV-2 Delta variant with a large 872 nt deletion, resulting in complete loss of ORF7a, ORF7b, and ORF8 genes. The index case carrying this deletion is unknown, and the standard sequencing pipeline may mask it with a stretch of N's. Further study is needed to investigate the effects of this deletion on phenotype or immune evasion.
Article
Chemistry, Medicinal
Daniel Umereweneza, Jackson T. Molel, Joanna Said, Yoseph Atilaw, Theoneste Muhizi, Edward Trybala, Tomas Bergstrom, Adolf Gogoll, Mate Erdelyi
Summary: The methanol root extract of Clerodendrum myricoides yielded two new and two known iridoid glycosides, which showed strong antiviral activity against human respiratory syncytial virus. Compound 2 exhibited the highest virus inhibition, while compound 1, 3, and 4 displayed weaker antiviral and cytotoxic activities.
Article
Virology
Petra Bergstrom, Edward Trybala, Charlotta E. Eriksson, Maria Johansson, Tugce Munise Satir, Sibylle Widehn, Stefanie Fruhwurth, Wojciech Michno, Faisal Hayat Nazir, Jorg Hanrieder, Soren Riis Paludan, Lotta Agholme, Henrik Zetterberg, Tomas Bergstrom
Summary: The study demonstrates that human induced pluripotent stem cells (iPSCs) differentiated into cortical neurons can be infected with clinical strains of HSV-1 and HSV-2. While both viruses decrease the viability of neuroprogenitors, cortical neurons show resilience to infection. However, in functional neurons, both HSV-1 and HSV-2 affect gene expression of synaptic activity markers and negatively impact synaptic activity.
Article
Virology
Maciej Szewczyk, Krzysztof Lepek, Sabina Nowak, Malgorzata Witek, Anna Bajcarczyk, Korneliusz Kurek, Przemyslaw Stachyra, Robert W. Myslajek, Boguslaw Szewczyk
Summary: African swine fever (ASF) caused by a DNA virus is a serious disease affecting suids, particularly in Eastern and Central Europe. Research suggests that wolves, known for high daily movement and long-range dispersal ability, may not be indirect vectors for ASFV transmission.
Article
Virology
Beata Gromadzka, Milena Chraniuk, Lilit Hovhannisyan, Karolina Uranowska, Boguslaw Szewczyk, Magdalena Narajczyk, Miroslawa Panasiuk
Summary: Our findings suggest that vaccination with triple H5N1/ NA-HA-M1 virus-like particles (VLPs) can generate broad-spectrum universal anti-HA-stalk antibodies in hens. Furthermore, these antibodies can recognize HA-stalk recombinant proteins from homologous H5N1 and heterologous H7N9 AIVs, as well as from the heterosubtypic human H1N1 influenza strain.
Article
Chemistry, Multidisciplinary
Scott E. Guimond, Courtney J. Mycroft-West, Neha S. Gandhi, Julia A. Tree, Thuy T. Le, C. Mirella Spalluto, Maria Humbert, Karen R. Buttigieg, Naomi Coombes, Michael J. Elmore, Matthew Wand, Kristina Nystrom, Joanna Said, Yin Xiang Setoh, Alberto A. Amarilla, Naphak Modhiran, Julian D. J. Sng, Mohit Chhabra, Paul R. Young, Daniel J. Rawle, Marcelo A. Lima, Edwin A. Yates, Richard Karlsson, Rebecca L. Miller, Yen-Hsi Chen, Ieva Bagdonaite, Zhang Yang, James Stewart, Dung Nguyen, Stephen Laidlaw, Edward Hammond, Keith Dredge, Tom M. A. Wilkinson, Daniel Watterson, Alexander A. Khromykh, Andreas Suhrbier, Miles W. Carroll, Edward Trybala, Tomas Bergstrom, Vito Ferro, Mark A. Skidmore, Jeremy E. Turnbull
Summary: This study found that the synthetic HS mimetic pixatimod can interact with the receptor binding domain of the SARS-CoV-2 spike protein and directly inhibit its binding to ACE2. The results show that pixatimod can effectively inhibit the infection of multiple clinical isolates of SARS-CoV-2 virus and has broad potency against various variants. In a mouse model, pixatimod also reduces viral titers and weight loss. This study provides strong evidence for the potential use of synthetic HS mimetics as a therapeutic approach for COVID-19.
ACS CENTRAL SCIENCE
(2022)
Article
Plant Sciences
Thobias M. Kalenga, Jackson T. Mollel, Joanna Said, Andreas Orthaber, Jas S. Ward, Yoseph Atilaw, Daniel Umereweneza, Monica M. Ndoile, Joan J. E. Munissi, Kari Rissanen, Edward Trybala, Tomas Bergstroem, Stephen S. Nyandoro, Mate Erdelyi
Summary: Two new modified ent-abietane diterpenoids, zanzibariolides A and B, and two known triterpenoids, simiarenol and fi-amyrin, were isolated from the leaf extract of Suregada zanzibariensis. The structures of these compounds were determined using NMR, MS, and X-ray crystallography. The crude extract showed inhibitory activity against HSV-2 and exhibited toxicity on GMK AH1 cells. However, the isolated compounds showed no anti-HSV-2 activity and showed insignificant toxicity against GMK AH1 cells.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Virology
Yara Abidine, Lifeng Liu, Oskar Wallen, Edward Trybala, Sigvard Olofsson, Tomas Bergstrom, Marta Bally
Summary: This study reveals the mechanisms of virus diffusion regulated by cell-surface GAGs, showing that different GAGs have different effects on virus diffusion and internalization. The findings provide important insights into the process of viral entry.
Article
Plant Sciences
Emanuel T. Mahambo, Colores Uwamariya, Masum Miah, Leandro da Costa Clementino, Luis Carlos Salazar Alvarez, Gabriela Paula Di Santo Meztler, Edward Trybala, Joanna Said, Lianne H. E. Wieske, Jas S. Ward, Kari Rissanen, Joan J. E. Munissi, Fabio T. M. Costa, Per Sunnerhagen, Tomas Bergstrom, Stephen S. Nyandoro, Mate Erdelyi
Summary: Six new crotofolane diterpenoids (1-6) and 13 known compounds (7-19) were isolated from Croton kilwae. The structures of the new compounds were determined by analyzing spectroscopic and mass spectrometric data. Some of the compounds exhibited antiviral, antibacterial, and antimalarial activities.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Virology
Sigvard Olofsson, Marta Bally, Edward Trybala, Tomas Bergstrom
Summary: N- and O-glycans are important components of viral envelope glycoproteins. O-glycosylation can result in functional diversity due to different human polypeptide O-acetylgalactosaminyl transferases. O-glycans play roles in viral life cycle and colonization of host, affecting interactions between viruses and their hosts. Conserved solitary O-glycans are crucial for viral uptake in target cells.
ANNUAL REVIEW OF VIROLOGY
(2023)
Article
Virology
Naiqing Xu, Xinen Tang, Xin Wang, Miao Cai, Xiaowen Liu, Xiaolong Lu, Shunlin Hu, Min Gu, Jiao Hu, Ruyi Gao, Kaituo Liu, Yu Chen, Xiufan Liu, Xiaoquan Wang
Summary: This study found that the H9N2 subtype avian influenza virus has a high airborne transmissibility, while the H7N9 virus does not. The Hemagglutinin protein of the H9N2 virus was found to play a key role in replication, stability, and airborne transmission.
Article
Virology
Samar S. Ewies, Sabry M. Tamam, Ahmed S. Abdel-Moneim, Sherin R. Rouby
Summary: Contagious ecthyma (CE) is a highly contagious viral disease of sheep and goats worldwide. The study provided a clinical description of CE and screened for genetic variation in the B2L gene. Infected sheep exhibited anorexia and oral lesions, while inoculated chicken embryos showed pock lesions. The B2L gene was successfully amplified and found to be highly conserved.
Article
Virology
Yigal Farnoushi, Dan Heller, Avishai Lublin
Summary: In recent years, new variants of avian reovirus (ARV) have caused a variety of symptoms in chickens worldwide, including viral arthritis/tenosynovitis. This study analyzed emerging ARV variants in Israel and found significant genetic diversity. Most ARV isolates in Israel belonged to genotypic cluster 5 (GC5). The study suggests that Israel has not experienced the emergence of new ARV variants since the introduction of the live vaccine (ISR-7585), but ongoing monitoring is needed due to the continuous emergence of ARV variants.
Article
Virology
Shigeru Tajima, Michiyo Kataoka, Yuki Takamatsu, Hideki Ebihara, Chang-Kweng Lim
Summary: Yokose virus (YOKV), a bat-associated flavivirus, was found to replicate at a slower rate in mosquito cells compared to other mosquito-borne flaviviruses. Specific nucleotide mutations in the virus were identified to enhance its proliferation ability in mosquito cells.
Article
Virology
Alejandra Borjabad, Baojun Dong, Wei Chao, David J. Volsky, Mary Jane Potash
Summary: This study investigated HIV brain disease using a mouse model, and found that poly I:C can reverse associated cognitive impairment and reduce virus burden. The results also revealed transcriptional changes related to neuronal function and innate immune responses.
Article
Virology
Ching-Hung Lin, Feng-Cheng Hsieh, Meilin Wang, Chieh Hsu, Hsuan-Wei Hsu, Chun-Chun Yang, Cheng-Yao Yang, Hung-Yi Wu
Summary: This study demonstrates that the synthesis of coronavirus subgenomic mRNA is not solely determined by the sequence homology between the leader TRS and TRS-B, but also by the disassociation of the coronavirus polymerase from the viral genome. This finding provides a new insight into the transcription mechanism of coronaviruses.
Article
Virology
Nicholas S. Kron, Benjamin W. Neuman, Sathish Kumar, Patricia L. Blackwelder, Dayana Vidal, Delphina Z. Walker-Phelan, Patrick D. I. Gibbs, Lynne A. Fieber, Michael C. Schmale
Summary: Two recent studies documented the genome of a novel virus in marine animals, finding that the virus is widespread in apparently healthy animals but not highly expressed in neurons. The studies also identified viral replication factories and high levels of defective genomes in chronically infected animals.
Article
Virology
Andrew M. Ramey, Laura C. Scott, Christina A. Ahlstrom, Evan J. Buck, Alison R. Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson
Summary: We successfully detected and characterized highly pathogenic avian influenza viruses in hunter-harvested wild waterfowl samples from western Alaska. Genomic analysis revealed three independent viral introductions into Alaska. Our findings demonstrate the utility and potential limitations of using molecular processing approaches directly on original swab samples for viral research and monitoring.
Article
Virology
Ting Gong, Dongdong Wu, Yongzhi Feng, Xing Liu, Qi Gao, Xiaoyu Zheng, Zebu Song, Heng Wang, Guihong Zhang, Lang Gong
Summary: This study discovered that quercetin can inhibit PEDV replication both in vivo and in vitro, and alleviate the clinical symptoms and intestinal injury caused by the virus. This provides a new direction for the development of PED antiviral drugs.
Article
Virology
Min Zhu, Hao Zeng, Jianqiao He, Yaohui Zhu, Pingping Wang, Jianing Guo, Jinfan Guo, Huabo Zhou, Yifeng Qin, Kang Ouyang, Zuzhang Wei, Weijian Huang, Ying Chen
Summary: The reassortment between avian H9N2 and Eurasian avian-like (EA) H1N1 viruses may have potentially changed from avian-to-mammals adaptation. This study found that the introduction of EA H1N1 internal genes into H9N2 virus restored the replication capability and resulted in extreme virulence in some cases. This raises new concerns for public health due to the possible coexistence of H9N2 and EA H1N1 viruses in dogs.
