Article
Cell Biology
Daniela Eletto, Francesca Reppucci, Agostino Ronga, Vincenzo Altieri, Sergio Brongo, Rosanna Martinelli, Margot De Marco, Liberato Marzullo
Summary: Diuron, a substituted urea herbicide, may induce bladder carcinogenesis by involving the BAG3 protein, which could potentially serve as an early biomarker for damage caused by chronic exposure to Diuron.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Mateja Erdani Kreft, Alexander A. Mironov, Samo Hudoklin
Summary: The Golgi complex undergoes structural remodeling during the differentiation of urothelial cells, which contributes to the formation of the blood-urine barrier. Uroplakins (UPs), the most abundant membrane proteins in differentiated urothelial cells, play a crucial role in the barrier's formation by their structural organization and hindering endocytosis. Golgi fragmentation is necessary for the delivery of specific differentiation cargos to the plasma membrane and cell-cell communication. Understanding the contribution of the Golgi complex to the blood-urine barrier is important for both normal urothelial cells and cancer cells.
HISTOCHEMISTRY AND CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Karim Mrouj, Nuria Andres-Sanchez, Geronimo Dubra, Priyanka Singh, Michal Sobecki, Dhanvantri Chahar, Emile Al Ghoul, Ana Bella Aznar, Susana Prieto, Nelly Pirot, Florence Bernex, Benoit Bordignon, Cedric Hassen-Khodja, Martin Villalba, Liliana Krasinska, Daniel Fisher
Summary: Ki-67, while not necessary for cell proliferation, plays a crucial role in tumor initiation, growth, and metastasis. Its absence leads to changes in the transcriptome and immune response, making cancer cells more susceptible to drugs and immune attacks.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Nazila V. Jafari, Jennifer L. Rohn
Summary: This study developed an improved 3D urine-tolerant human urothelial model, which demonstrated the differentiation status needed for barrier function and exhibited a similar immune response to urinary tract infection in humans.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Oncology
Qixin Liu, Ziheng Peng, Liangfang Shen, Lin Shen
Summary: Ki-67 overexpression is associated with poor overall survival rates in melanoma patients, but not correlated with PFS and RFS rates. Ki-67 expression levels are associated with tumor thickness.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sydney R. Grant, Li Tang, Lei Wei, Barbara A. Foster, Gyorgy Paragh, Wendy J. Huss
Summary: More than 80,000 new cases of bladder cancer are estimated to be diagnosed in 2023, but the 5-year survival rate has remained unchanged for decades, highlighting the need for prevention. Mutations in cancer-driving genes in normal urothelium have shown a significant correlation with bladder cancer tissue, providing evidence for mutation hotspots in assessing cancer risk. A machine learning model using personal risk factors and mutations within bladder cancer hotspots has shown promising results in predicting high- vs. low-risk normal urothelium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Li Zhang, Mengyi Shen, Dingyi Zhang, Xin He, Qinglin Du, Nian Liu, Xiaohua Huang
Summary: A nomogram based on dual-sequence MRI radiomic features and clinical characteristics was developed to assess Ki-67 expression in breast cancer. It showed improved prediction efficiency compared to single-sequence models and clinical models.
JOURNAL OF MAGNETIC RESONANCE IMAGING
(2023)
Review
Biochemistry & Molecular Biology
Lucy Remnant, Natalia Y. Kochanova, Caitlin Reid, Fernanda Cisneros-Soberanis, William C. Earnshaw
Summary: Ki-67 is a well-known marker protein used to identify proliferating cells, but our understanding of its molecular biology and biochemistry is incomplete, and its exact molecular function remains mysterious. Recent studies have revealed that Ki-67 is dispensable for cell proliferation, contrary to previous understanding. Its intrinsically disordered structure may play a role in controlling liquid-liquid phase separation of nucleolar proteins and RNAs, aiding in the assembly of the enigmatic mitotic chromosome periphery compartment.
Review
Oncology
Matthew G. Davey, Sean O. Hynes, Michael J. Kerin, Nicola Miller, Aoife J. Lowery
Summary: In breast cancer development, the expression of Ki-67 is a valuable indicator for prognosis and treatment decision making. The advent of molecular medicine has transformed breast cancer management by recognizing the heterogeneity of the disease and using a combination of factors for substratification and personalization of treatment modalities. Routine evaluation of Ki-67 as a proliferation marker in breast cancer patients has limitations, but promising strategies are being explored to enhance its utility in future oncology.
Article
Cell Biology
Koji Okudela, Tetsukan Woo, Yusuke Saigusa, Hiromasa Arai, Mai Matsumura, Hideaki Mitsui, Misaki Sugiyama, Motoki Sekiya, Yoshihiro Ishikawa, Hisashi Oshiro, Yoichi Kameda, Kenichi Ohashi
Summary: This study optimized a procedure for measuring the Ki-67 index in lung adenocarcinoma and established the best cut-off value. Using the SP6 antibody for immunohistochemical staining, they successfully developed an optimal procedure for determining the Ki-67 labeling index. They identified an optimal cut-off value of 0.12 and achieved a coverage probability >70% and a confidence interval of <0.05 in over 90% of cases.
Article
Endocrinology & Metabolism
L. Samuel Hellgren, Adam Stenman, Johan O. Paulsson, Anders Hoog, Catharina Larsson, Jan Zedenius, C. Christofer Juhlin
Summary: The study aimed to assess the potential value of Ki-67 as an adjunct tool for distinguishing between follicular thyroid carcinoma (FTC) and adenoma (FTA) and predicting prognosis. The findings suggest that the Ki-67 labeling index can be used to separate FTC from FTA and identify high-risk FTCs. This marker has the potential to be beneficial in the histopathological assessment of follicular thyroid tumors if reproduced in international series.
ENDOCRINE PATHOLOGY
(2022)
Article
Oncology
Soon Bo Choi, Jung Min Park, Jee Hyun Ahn, Jieon Go, Jeeye Kim, Hyung Seok Park, Seung Il Kim, Byeong-Woo Park, Seho Park
Summary: This study aimed to explore the relationship between Ki-67 level and the prognosis of breast cancer patients, regardless of the timing of Ki-67 testing. The results showed that patients with higher Ki-67 levels had poorer survival, larger tumors, higher grade, and more aggressive treatment, regardless of when the testing was conducted.
BREAST CANCER RESEARCH AND TREATMENT
(2022)
Article
Oncology
Pingping Wang, Hai Li, Yu Hu, Xiaojing Peng, Xinhua Ye, Di Xu, Ao Li
Summary: This study found a moderate correlation between vascular density grade and Ki-67 LI in soft tissue sarcoma, with other ultrasound features showing no correlation. The optimal cut-off point for distinguishing low and high proliferation groups was determined to be grade II. The area under the ROC curve was 0.74, with a sensitivity of 60.6% and specificity of 78.6%.
FRONTIERS IN ONCOLOGY
(2021)
Article
Pharmacology & Pharmacy
B. Haznedar, O. Kaplan, F. Asir, I. S. Ermis
Summary: The aim of this study was to investigate the expression level of Ki-67 in nasal polyps using immunohistochemical method. The results showed abnormal blood parameters and histological changes in nasal polyps, including cellular degeneration and leukocyte infiltration. Ki-67 expression was observed in mucosal epithelial cells, vascular endothelial cells and plasma cells. In conclusion, epithelial degeneration and leukocyte infiltration may contribute to the formation of nasal adenoma, and Ki-67 expression could be a diagnostic tool.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2023)
Article
Medicine, General & Internal
Yu Meng, Quan Quan, Fenfen Zhang, Yao Liu, Siling Ren, Xiaoling Mu
Summary: This study investigated the prognostic value of the Ki-67 index in endometrial stromal sarcoma and identified a cut-off value of 35% for predicting recurrence. High Ki-67 index and ovarian preservation were significantly associated with shorter progress free survival. The Ki-67 index could serve as a useful prognostic marker in endometrial stromal sarcoma.
FRONTIERS IN MEDICINE
(2022)
Article
Plant Sciences
Natasa Resnik, Anja Mavric, Darja Kese, Peter Veranic, Dasa Zupancic
Article
Plant Sciences
Roghayeh Imani, Meysam Pazoki, Dasa Zupancic, Mateja Erdani Kreft, Veronika Kralj-Iglic, Peter Veranic, Ale Iglic
Article
Plant Sciences
Dasa Zupancic, Mateja Erdani Kreft, Maja Grdadolnik, Dimitar Mitev, Ales Iglic, Peter Veranic
Article
Plant Sciences
Dasa Zupancic, Marjeta Tercelj, Bojan Strus, Peter Veranic
Article
Anatomy & Morphology
Dasa Zupancic, Rok Romih, Horst Robenek, Kristina Zuzek Rozman, Zoran Samardzija, Rok Kostanjsek, Mateja Erdani Kreft
MICROSCOPY RESEARCH AND TECHNIQUE
(2014)
Article
Plant Sciences
Dasa Zupancic, Mateja Erdani Kreft, Rok Romih
Review
Biotechnology & Applied Microbiology
Gilho Lee, Rok Romih, Dasa Zupancic
BIOMED RESEARCH INTERNATIONAL
(2014)
Article
Cell Biology
Dasa Zupancic, Mateja Erdani Kreft, Igor Sterle, Rok Romih
EUROPEAN JOURNAL OF HISTOCHEMISTRY
(2020)
Article
Oncology
Dasa Zupancic, Jelena Korac-Prlic, Mateja Erdani Kreft, Lucija Frankovic, Katarina Vilovic, Jera Jeruc, Rok Romih, Janos Terzic
Article
Cell Biology
Dasa Zupancic, Rok Romih
Summary: The urothelium of the urinary bladder acts as a permeability barrier, with the ability to respond quickly to injury and regenerate. The review discusses the importance of key markers and potential treatments for bladder diseases. The use of immunohistochemistry is emphasized in advancing our understanding and management of bladder disorders.
EUROPEAN JOURNAL OF HISTOCHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Larisa Tratnjek, Jera Jeruc, Rok Romih, Dasa Zupancic
Summary: Vitamin A and its derivatives, particularly retinoic acid, show promising potential in the chemoprevention and treatment of bladder cancer, but their translation to clinical practice is currently limited.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Dasa Zupancic, Peter Veranic
Summary: Nanodiamonds (NDs) are carbon nanomaterials with excellent properties, making them promising for biomedical applications. However, selectively targeting desired biomolecules within complex biological systems using NDs remains challenging. This review presents the synthesis, properties, and functionalization of NDs, as well as recent advances in their use for improving the treatment of bladder cancer and bacterial cystitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Developmental Biology
Sara Mugerli, Dasa Zupancic, Rok Romih, Miha Lucovnik
Summary: This study demonstrated the reduction of endothelial glycocalyx in pregnant patients with severe preeclampsia using transmission electron microscopy.
Review
Biochemistry & Molecular Biology
Tanja Visnjar, Rok Romih, Dasa Zupancic
Article
Cell Biology
Dasa Zupancic, Katjusa Mrak Poljsak, Mateja Erdani Kreft
CELL BIOLOGY INTERNATIONAL
(2018)