Article
Microbiology
Dennis Maletich Junqueira, Caroline Tochetto, Tavis K. Anderson, Danielle Gava, Vanessa Haach, Mauricio E. Cantao, Amy L. Vincent Baker, Rejane Schaefer
Summary: This study analyzed a large number of H1N1pdm09 gene sequences from humans and swine globally between 2009 and 2022, and found that there was rapid transmission of the virus from humans to swine in Brazil during 2009-2011, but most of the infections in swine were self-limited with limited onward transmission. After 2012, there was a reduction in human-to-swine H1N1pdm09 transmissions in Brazil. The study highlights the dynamic interplay between human-to-swine transmission, antigenic drift, and the establishment of swine-to-swine transmission in shaping the evolution and persistence of H1N1pdm09 in swine populations.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Subuhi Sherwani, Mohd Wajid Ali Khan, Arshi Mallik, Mahvish Khan, Mohd Saleem, Mohamed Raafat, Ayed A. Shati, Noor Alam
Summary: This study investigated the seroprevalence of serum antibodies against the SARS-CoV-2 S1-RBD antigen in a population from the Hail region, KSA, during the pre-pandemic and early pandemic period. The results showed that subjects during the pandemic period exhibited significantly higher levels of serum antibodies than the pre-pandemic subjects. Additionally, a small number of pre-pandemic subjects also showed serum antibodies, suggesting prior exposure to other coronaviruses in the region. The study highlights the importance of developing better assays for surveillance, management, and future research.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Immunology
Veronica A. Ferrando, Marcel E. Friedrich, Shrey Gandhi, Alexander Mellmann, Doerthe Masemann, Anmari Christersson, Darisuren Anhlan, Linda Brunotte, Monika Stoll, Timm Harder, Martin Beer, Yvonne Boergeling, Stephan Ludwig
Summary: Influenza A viruses, including the H1N1pdm09 virus, cause recurrent epidemics and occasional pandemics in humans. The reassortment of these viruses in swine and their ability to replicate in human lung tissue suggest a possible zoonotic potential. Reassortment frequently occurs in the PB1, PA, and NA segments, resulting in the generation of reassortants that can reach high titers in swine lung cells.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Multidisciplinary Sciences
Kristien Van Reeth, Anna Parys, Jose Carlos Mancera Gracia, Ivan Trus, Koen Chiers, Philip Meade, Sean Liu, Peter Palese, Florian Krammer, Elien Vandoorn
Summary: This study demonstrates that sequential vaccination with diverse H1N1 viruses in pigs induces a broad H1N1 antibody response, providing protection against multiple H1N1 strains in humans and pigs.
NATURE COMMUNICATIONS
(2023)
Article
Virology
Salin Sirinam, Supawat Chatchen, Watcharee Arunsodsai, Suriya Guharat, Kriengsak Limkittikul
Summary: This study investigated the seroprevalence of Zika virus infection in Samut Songkhram, Thailand, during the 2016 outbreak. The findings showed a seroprevalence rate of 15.1-17.8% with no significant change over the year. The study confirmed the occurrence of occult Zika virus infections in the community, which could contribute to the spread of the virus in vulnerable groups.
Article
Biotechnology & Applied Microbiology
Zhihong Qian, Dylan H. H. Morris, Annika Avery, Karen A. A. Kormuth, Valerie Le Sage, Michael M. M. Myerburg, James O. O. Lloyd-Smith, Linsey C. C. Marr, Seema S. S. Lakdawala
Summary: Respiratory viruses can be transmitted through contaminated surfaces, or fomites. This study examined the stability of the H1N1 virus on different surface materials at varying humidities. The results showed that the virus was rapidly inactivated on copper, but remained stable on plastics, steel, aluminum, and glass. The stability of the virus was more influenced by the respiratory secretion of the host than the surface material.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Virology
Gloria Perez-Rubio, Marco Antonio Ponce-Gallegos, Bruno Andre Dominguez-Mazzocco, Jaime Ponce-Gallegos, Roman Alejandro Garcia-Ramirez, Ramces Falfan-Valencia
Summary: Research has shown that genetic susceptibility to Influenza A virus infection is influenced by multiple pathways, including innate immune response, complement activation, and variants in inflammatory response genes. Specific gene polymorphisms such as SNPs play key roles in host immune responses, affecting viral replication and host response, representing important factors in genetic susceptibility to IAV infection.
Article
Immunology
Krista Salo-Tuominen, Tamara Teros-Jaakkola, Laura Toivonen, Helena Ollila, Paivi Rautava, Minna Aromaa, Elina Lahti, Niina Junttila, Ville Peltola
Summary: The study investigated the association between parental socioeconomic and psychosocial factors and the uptake of the 2009 pandemic influenza vaccine in children. It found that children of younger and less educated mothers had a lower rate of vaccine uptake. However, the individual or relational psychosocial well-being of parents was not associated with children's vaccination.
Article
Immunology
Jiapei Yu, Hui Li, Ju Jia, Zhisheng Huang, Shuai Liu, Ying Zheng, Shengrui Mu, Xiaoyan Deng, Xiaohui Zou, Yeming Wang, Xiao Shang, Dan Cui, Lixue Huang, Xiaoxuan Feng, William J. Liu, Bin Cao
Summary: Despite the use of vaccines and anti-viral drugs, influenza A virus still poses a significant epidemic risk to global public health. Through single-cell transcriptome analyses, we have identified the high heterogeneity of susceptibility to viral infection and biological responses in different subsets of CD4(+) T and CD8(+) T cells. Effector memory CD8(+) T cells (CD8(+) T-EM) were found to be the most susceptible subset to pandemic influenza A virus infection among primary human T cells. Non-productive infection was established in CD8(+) T-EM and naive CD8(+) T cells, indicating the mechanisms of intracellular antiviral activities in human T cells.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Virology
Shannon R. Christensen, Emily T. Martin, Joshua G. Petrie, Arnold S. Monto, Scott E. Hensley
Summary: The HA stalk domain of the pH1N1 virus has remained antigenically stable despite the presence of high levels of HA stalk antibodies within the human population. The pH1N1 HA stalk has not undergone antigenic drift in the past decade, as serum antibodies from 230 humans were unable to distinguish between the 2009 and 2019 HA stalks.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Seungwon Kim, Margaret Carrel, Andrew Kitchen
Summary: Identifying the spatial patterns of genetic structure of influenza A viruses is important for understanding their spread. This study analyzed the genetic sequences of the A/H1N1pdm09 virus in mainland China and found that there is high genetic similarity among viruses within small geographic regions, but broad-scale genetic differentiation. Local viral circulation has a greater impact on the spatial genetic structure of the virus than countrywide viral mixing and gene flow.
Article
Business, Finance
Di Gong, Tao Jiang, Liping Lu
Summary: This paper empirically analyzes the impact of pandemic on the contracting of bank loans. Using data on syndicated loans during the season of H1N1 Swine Flu, we find that more flu is associated with higher loan spreads and smaller loan size. The adverse impact of pandemic was alleviated by the approval of vaccines.
FINANCE RESEARCH LETTERS
(2021)
Article
Virology
Nima Ghalekhani, Saied Bokaie, Sana Eybpoosh, Hesameddin Akbarein, Hamid Sharifi
Summary: Using Bayesian phylogeography analysis, this study investigates the time and path of transmission of H1N1 serotype influenza A viruses in Iran and neighboring countries. The study finds that the age of emergence of H1N1 virus in Iran is older compared to neighboring countries, and Tehran seems to have played a key role in its transmission within Iran. Continuous monitoring and screening of H1N1 virus, along with addressing gaps and shortages in virus control, are important measures to be taken.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Vaclav Brazda, Otilia Porubiakova, Alessio Cantara, Natalia Bohalova, Jan Coufal, Martin Bartas, Miroslav Fojta, Jean-Louis Mergny
Summary: The study identified multiple potential G-quadruplex forming sequences in the G4-EA-H1N1 genomes, which could serve as potential therapeutic targets.
Article
Microbiology
Jing-ying Sun, Chun-yan Guo, Guo-rong Wang, Li-ting Yan, Qing Feng, Yan Li, Xue-ping Huo, Xin Xie, Jun Hu, Li-jun Sun
Summary: Our previous studies found that the H1-50 monoclonal antibody cross-reacts with pancreatic tissue and islet beta-cells, and further investigation revealed that it binds to PHB protein. We screened the binding epitopes using a phage 12-peptide library and identified a specific binding sequence of H1-50 mAb to influenza A virus HA. The study provides new insights into the role of influenza virus in type 1 diabetes.
CURRENT MICROBIOLOGY
(2023)
Article
Nursing
Evgnosia Effraimidou, Dimitrios C. Cassimos, Snezana Medic, Maria Topalidou, Maria Theodoridou, Helena C. Maltezou
Summary: Although all European countries have vaccination policies for children, there are no comprehensive studies of pediatric vaccination programs in Europe. The study found variations in vaccination programs and policies among European countries, indicating the need for a consensus-based vaccination program.
JOURNAL OF CHILD HEALTH CARE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Theodoros Tasopoulos, Georgia Vrioni, Olga Naka, Theodora Diamantatou, Panagiotis Zoidis, Athanasios Tsakris
Summary: This in vitro study investigated the adherence of Candida albicans to five long-term silicone-based soft denture lining materials using artificial saliva. The results showed that Molloplast B had significantly earlier adherence of C. albicans cells compared to the other materials, and the number of cells increased over time. The study suggests that long-term silicone denture liners accumulate a significant amount of C. albicans and their coverage increases progressively over time.
JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY
(2023)
Review
Infectious Diseases
Maria Kantzanou, Maria A. Karalexi, Kalliopi Theodoridou, Evangelos Kostares, Georgia Kostare, Thalia Loka, Georgia Vrioni, Athanassios Tsakris
Summary: This review analyzed published data on the prevalence of Leishmania spp. infection among people with HIV and found a prevalence rate of 6%. The study also identified age, sex, and CD4 cell count as determinants that affect the prevalence rates. The research provides important insights into the burden of Leishmaniasis in the context of HIV.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Editorial Material
Infectious Diseases
Athanasios Tsakris, James Hurley, Alan Johnson, Russell Lewis
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Immunology
Helena C. Maltezou, Sophia Hatziantoniou, Kalliopi Theodoridou, Konstantinos Vasileiou, Cleo Anastassopoulou, Athanasios Tsakris
Summary: The aim of this study was to estimate the rates of anaphylaxis following mRNA COVID-19 vaccination in children and adolescents in Europe. The results showed that the rate of anaphylaxis after mRNA COVID-19 vaccination in children and adolescents is low, and continuous monitoring is needed to guide vaccination policies.
Review
Virology
Helena C. Maltezou, Amalia Papanikolopoulou, Sofia Vassiliu, Kalliopi Theodoridou, Georgia Nikolopoulou, Nikolaos V. Sipsas
Summary: The COVID-19 pandemic has significantly affected the circulation, seasonality, and morbidity of respiratory viruses. Co-infections of SARS-CoV-2 and influenza were mainly reported during the first wave of the pandemic. However, the overall incidence of co-infections may be higher due to limited testing during that period. While animal models indicate severe lung pathology and high fatality, the clinical course and prognosis of co-infected patients remain inconclusive in human cases. It is important to consider the timing of each respiratory virus infection, but such information is lacking in reported cases. With the evolving epidemiology and availability of vaccines and treatments, early findings should not be extrapolated to the present times. Multiplex real-time PCR-based assays can improve diagnosis, infection control, and surveillance. Vaccination against both COVID-19 and influenza is crucial for high-risk groups. Further studies are needed to understand the impact and prognosis of SARS-CoV-2 and respiratory virus co-infections in the coming years.
Article
Microbiology
Maria Katsiari, Angeliki Mavroidi, Nikolaos Kesesidis, Eleftheria Palla, Konstantina Zourla, Kyriakos Ntorlis, Konstantinos Konstantinidis, Maria Laskou, Konstantinos Strigklis, Anastasios Sakkalis, Charikleia Nikolaou, Evangelia D. Platsouka, Ioannis Karakasiliotis, Georgia Vrioni, Athanasios Tsakris
Summary: The first five ICU cases of C. auris in Greece were reported. All C. auris isolates showed resistance to fluconazole, and three of them exhibited resistance to amphotericin B. Environmental screening also indicated the dissemination of C. auris in the ICU. The study confirms the emergence of C. auris as a yeast pathogen in the region during the COVID-19 pandemic.
Article
Health Care Sciences & Services
Maria Mavrouli, Spyridon Mavroulis, Efthymios Lekkas, Athanassios Tsakris
Summary: On 6 February 2023, two major earthquakes struck Turkey and Syria, causing severe damage to structures and infrastructure in densely populated areas. The authors visited the affected area shortly after the earthquakes to assess the potential impact on public health. Field data revealed that risk factors for infectious diseases were present in the affected residential areas from the early stages of the emergency. Collapsed health facilities, cold winter conditions, destroyed infrastructure, overcrowded shelters, poor sanitation, and adverse socio-economic conditions, along with ongoing crises and disasters, could worsen the public health situation and delay the recovery process. Efficient disease surveillance and other prevention measures are crucial for early warning and management of infectious diseases in earthquake-affected areas.
Article
Immunology
Caterina Ledda, Venerando Rapisarda, Helena C. Maltezou
Summary: Despite evidence of the benefits of COVID-19 vaccination, vaccine hesitancy and refusal among healthcare personnel have become a major concern. A survey among Italian healthcare personnel who refused COVID-19 vaccination and were suspended from work revealed that they had good knowledge of occupational vaccinations but strong anti-vaccination beliefs. They were against mandatory vaccination policies and expressed doubts about the expected benefits and safety of vaccines.
Article
Infectious Diseases
Konstantina Gartzonika, Lida Politi, Angeliki Mavroidi, Andreas G. Tsantes, Nicholas Spanakis, Efthalia Priavali, Georgia Vrioni, Athanasios Tsakris
Summary: This study describes the epidemiological and molecular characteristics of a large-scale outbreak of NDM-1-producing Enterobacter cloacae complex in Greece, which is the largest clonal outbreak of its kind in Europe. The presence of NDM-1 gene was confirmed in the isolates and it was found to be associated with other resistance genes through genetic sequencing.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Review
Biochemistry & Molecular Biology
Fotini Boufidou, Snezana Medic, Vicky Lampropoulou, Nikolaos Siafakas, Athanasios Tsakris, Cleo Anastassopoulou
Summary: We are reviewing the current state of knowledge on the virological and immunological correlates of long COVID, focusing on the possible association between SARS-CoV-2 reinfections and long COVID. The severity of reinfections depends on the severity of the initial episode and a combination of genetic factors and the pathogenicity of the variant. The risk of long COVID and complications increases with the number of SARS-CoV-2 infections, especially in the elderly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Vassiliki C. Pitiriga, Myrto Papamentzelopoulou, Kanella E. Konstantinakou, Kalliopi Theodoridou, Irene V. Vasileiou, Athanasios Tsakris
Summary: The study compared the levels of T cell responses to SARS-CoV-2 between cohorts of subjects with hybrid immunity, vaccinated naive subjects, and convalescent unvaccinated subjects. The findings suggest that natural cellular immunity, either alone or combined with vaccination, confers stronger and more durable protection compared to vaccine-induced cellular immunity.
Article
Microbiology
Joseph Papaparaskevas, Alexandra Procopiou, John Routsias, Georgia Vrioni, Athanasios Tsakris
Summary: Brucellosis remains prevalent in Greece, with the majority of isolates identified as B. melitensis. All eight pathogenicity-associated genes were present in almost all Brucella isolates, though the ure gene was absent in some B. melitensis isolates.
Article
Infectious Diseases
Angeliki Mavroidi, Konstantina Gartzonika, Nick Spanakis, Elisavet Froukala, Christos Kittas, Georgia Vrioni, Athanasios Tsakris
Summary: In this study, comparative genomics of two representative outbreak isolates of multidrug-resistant Enterobacter cloacae complex were performed. It was found that these two isolates harbored multiple antibiotic resistance genes and various virulence factors, with differences in the genetic makeup of the bacterial cell surface and genomic islands. Additionally, diverse modes of acquisition and ongoing evolution of plasmids carrying beta-lactamase genes between the two strains were observed.
Review
Health Care Sciences & Services
Helena C. Maltezou, Caterina Ledda, Nikolaos V. Sipsas
Summary: This systematic review assessed the impact of COVID-19 on absenteeism among healthcare personnel. The findings showed that COVID-19 was a major driver of absenteeism among healthcare personnel, with the duration of absence varying based on different factors. COVID-19 vaccination, routine testing, and the use of personal protective equipment were found to significantly improve absenteeism. The study highlights the need for ongoing research and the establishment of networks to monitor morbidity and absenteeism among healthcare personnel in real-time and inform vaccination policies.