Article
Neurosciences
Natali L. Chanaday, Elena Nosyreva, Ok-Ho Shin, Hua Zhang, Iltan Aklan, Deniz Atasoy, Ilya Bezprozvanny, Ege T. Kavalali
Summary: The study found that acute ER Ca2+ depletion increases presynaptic Ca2+ levels and glutamate release, while chronic ER stress leads to syt7-dependent potentiation of spontaneous glutamate release. Inhibition of SOCE or syt7-driven fusion during ER stress can partially restore basal neurotransmission.
Article
Cell Biology
Camille S. Wang, Lisa M. Monteggia, Ege T. Kavalali
Summary: Calcium (Ca2+) signaling is tightly regulated within a presynaptic bouton, involving evoked, spontaneous, and baseline signals derived from multiple sources. These signals have non-overlapping domains within the synapse and interact through synaptic vesicle turnover.
Article
Neurosciences
Andreas T. Grasskamp, Meida Jusyte, Anthony W. McCarthy, Torsten W. B. Goetz, Susanne Ditlevsen, Alexander M. Walter
Summary: Synaptic transmission relies on the release of neurotransmitter from synaptic vesicles and its detection by postsynaptic receptors. There are two principal modes of transmission: action-potential evoked and spontaneous transmission. This study investigates the functional interdependence of these modes at individual synaptic contacts in Drosophila larval neuromuscular junctions. The results show that spontaneous activity can predict the responsiveness of synapses to action-potential stimulation.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Pao-Huan Chen, Cheng-Chih Chung, Yuan-Feng Lin, Yu-Hsun Kao, Yi-Jen Chen
Summary: The study showed that therapeutic trough levels of lithium can reduce the migration and collagen synthesis abilities of human cardiac fibroblasts by inhibiting SOCE and Orai1 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Baris Alten, Qiangjun Zhou, Ok-Ho Shin, Luis Esquivies, Pei-Yi Lin, K. Ian White, Ron Sun, Wendy K. Chung, Lisa M. Monteggia, Axel T. Brunger, Ege T. Kavalali
Summary: Recent studies have shown that mutations in the SNAP25 gene may cause developmental and epileptic encephalopathies, but the specific mechanisms are not yet clear. Research has found that mutations in SNAP25 can lead to related synaptic transmission phenotypes, but specific alterations in spontaneous neurotransmitter release are key factors in disease heterogeneity.
Article
Multidisciplinary Sciences
Susann Ludewig, Ulrike Herrmann, Kristin Michaelsen-Preusse, Kristin Metzdorf, Jennifer Just, Charlotte Bold, Ulrike C. Mueller, Martin Korte
Summary: The study reveals the essential role of the APP family, particularly the ectodomain APPs alpha, in neuronal Ca2+ handling. Additionally, the loss of APP and APLP2 is shown to impair Ca2+ handling, endoplasmic reticulum Ca2+ store refill, and synaptic plasticity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Camille S. Wang, Natali L. Chanaday, Lisa M. Monteggia, Ege T. Kavalali
Summary: This study used a fluorescent probe called iGluSnFR to investigate the organization of action potential-evoked and spontaneous neurotransmitter release within individual synapses. The results showed that action potential-evoked release is more susceptible to photobleaching, while spontaneous release is less affected.
Article
Biophysics
Lawrence Oprea, Nicolas Desjardins, Xiaoyu Jiang, Kushagra Sareen, James Q. Zheng, Anmar Khadra
Summary: This study developed a stochastic spatiotemporal computational model to simulate spontaneous Ca2+ local transients (SCaLTs) in isolated oligodendrocyte precursor cells. By comparing the model simulation with experimental data, the study found that SCaLTs exhibited similar characteristics between the two datasets, were mostly random, and encoded information in their frequency. The study also suggested that the slow baseline oscillations of SCaLTs could be attributed to the stochastic slow clustering of inositol-trisphosphate receptors.
BIOPHYSICAL JOURNAL
(2022)
Review
Cell Biology
Ewelina Latoszek, Magdalena Czeredys
Summary: The review highlights the essential role of SOCE in the physiology of neural stem cells and its dysregulation in HD pathology. Recent studies demonstrate the impact of elevated STIM2 expression on excessive Ca2+ entry in juvenile-onset HD-induced GABAergic cells. Progress in the in vitro differentiation of MSNs derived from various cell sources is discussed, along with the establishment of HD brain organoids for studying HD pathology and potential treatments. Stem cell therapy, particularly the transplantation of MSNs or their precursors, shows promise in restoring normal neural cell function in HD patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Editorial Material
Cell Biology
Jana Hartmann, Hsing-Jung Chen-Engerer, Arthur Konnerth
Summary: This study explores the critical role of Orai1 channels in hippocampal neurons, which contribute to the Ca2+ signal and synaptic plasticity associated with learning and memory. The findings contrast with previous reports emphasizing the roles of Orai2 channels and overlooking the functions of Orai1 channels in these neurons.
Article
Biochemistry & Molecular Biology
Silvija Jankeviciute, Natasa Svirskiene, Gytis Svirskis, Vilmante Borutaite
Summary: The study revealed that microglial cells exhibit changes in Ca2+ signaling when mitochondrial respiration is inhibited under both normoxic and mild-hypoxic conditions, and different effects are observed with the drugs metformin and rotenone. The fast action of metformin and rotenone in normoxia contrasts with their slow action during mild-hypoxia, suggesting activation of distinct signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Omar A. Ramirez, Alex Cordova, Mauricio Cerda, Pedro Lobos, Steffen Hartel, Andres Couve, Cecilia Hidalgo
Summary: Neuronal Ca2+ signals are essential for synaptic transmission and gene expression changes. Voltage-gated Ca2+ channels and N-methyl-D-aspartate receptors are important for Ca2+ entry during action potential firing. The endoplasmic reticulum (ER), including RyR and IP3R channels, contributes to Ca2+ signal generation in response to neuronal activity. The study investigates the role of RyR-mediated Ca2+ release in IP3-generated Ca2+ signals in dendrites and the potential impact of atlastin on Ca2+ signaling and ER-resident Ca2+ channel distribution.
Article
Biology
Alberto Coccarelli, Sanjay Pant
Summary: Agonist-induced Ca2+ signaling plays a vital role in regulating endothelial cell functions. This study establishes a minimalistic modeling framework based on data from isolated endothelial cells to quantitatively capture the cytosolic Ca2+ response to IP3 stimulation. The Ca2+-regulatory function of IP3Rs and parameter space are identified through experimental fitting and validation. This minimalistic model can serve as a reference framework for quantifying EC Ca2+ dynamics induced by IP3Rs activation.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Engineering, Multidisciplinary
Sergey A. Lobov, Ekaterina S. Berdnikova, Alexey I. Zharinov, Dmitry P. Kurganov, Victor B. Kazantsev
Summary: Mathematical and computer simulations of learning in neural networks have mostly focused on changes in synaptic weights. However, experimental data suggests that brain circuit plasticity also involves homeostatic and structural plasticity. This study proposes a model of structural plasticity based on activity-dependent appearance and disappearance of synaptic connections. The results show that adaptive rewiring can consolidate the effects of synaptic plasticity.
Article
Biology
Alexis Bedecarrats, Laura Puygrenier, John Castro O'Byrne, Quentin Lade, John Simmers, Romuald Nargeot
Summary: A study in isolated nervous system preparations of Aplysia reveals that a rhythmic signal based on intracellular calcium fluxes can act as an autonomous releaser for food-seeking behavior by driving motor output through voltage oscillations in a specific pair of interneurons.
Article
Multidisciplinary Sciences
Benjamin R. Nelson, Catherine A. Makarewich, Douglas M. Anderson, Benjamin R. Winders, Constantine D. Troupes, Fenfen Wu, Austin L. Reese, John R. McAnally, Xiongwen Chen, Ege T. Kavalali, Stephen C. Cannon, Steven R. Houser, Rhonda Bassel-Duby, Eric N. Olson
Article
Biology
Austin L. Reese, Ege T. Kavalali
Article
Cell Biology
Katie Schaukowitch, Austin L. Reese, Seung-Kyoon Kim, Gokhul Kilaru, Jae-Yeol Joo, Ege T. Kavalali, Tae-Kyung Kim
Article
Neurosciences
Manjot Bal, Jeremy Leitz, Austin L. Reese, Denise M. O. Ramirez, Murat Durakoglugil, Joachim Herz, Lisa M. Monteggia, Ege T. Kavalali
Article
Ecology
Raymond Barbehenn, Chris Dukatz, Chris Holt, Austin Reese, Olli Martiskainen, Juha-Pekka Salminen, Lynn Yip, Lan Tran, C. Peter Constabel
Article
Multidisciplinary Sciences
Anna K. Koster, Austin L. Reese, Yuri Kuryshev, Xianlan Wen, Keri A. McKiernan, Erin E. Gray, Caiyun Wu, John R. Huguenard, Merritt Maduke, J. Du Bois
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
