Article
Immunology
Shengtao Fan, Yun Liao, Guorun Jiang, Lichun Wang, Heng Zhao, Li Yu, Xingli Xu, Dandan Li, Ying Zhang, Qihan Li
Summary: The study developed a bivalent inactivated EV71/CA16 vaccine that, when administered via the intradermal route, activated innate immunity and induced neutralizing antibodies and specific T cellular responses in adult mice. This successful induction of specific protective antiviral immunity suggests that immunization with this vaccine elicits effective immunity against EV71 and CA16 infection.
Article
Immunology
Yaping Chen, Yanhui Xiao, Ying Ye, Feng Jiang, Hanqing He, Linyun Luo, Haiping Chen, Lubin Shi, Qiuyue Mu, Wei Chen, Xue Guo, Min Zhang, Jun Li, Qinghu Guan, Zhiping Chen, Xiaoming Yang
Summary: This study demonstrates that in infants, coadministration of an inactivated enterovirus 71 vaccine (EV71 vaccine) with trivalent split-virion inactivated influenza vaccine (IIV3) is safe and does not interfere with immunogenicity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Eun-Je Yi, Young-In Kim, Seung-Yeon Kim, Sung Hyun Ahn, Hyoung Jin Lee, Bohyun Suh, Jaelim Yu, Jeehye Park, Yoon Jung Lee, Eunju Jung, Sun-Young Chang
Summary: Hand-foot-and-mouth disease (HFMD) is a viral infectious disease mainly caused by coxsackievirus (CV) and enterovirus (EV) in children under 5 years old. In the absence of efficient therapeutics, vaccines play a crucial role in preventing HFMD. A bivalent vaccine is needed to provide broad coverage against both CV and EV. This study used Mongolian gerbils as an animal model to investigate the efficacy of a bivalent inactivated EV71 C4a and inactivated CVA16 vaccine. The results showed that the bivalent vaccine induced increased production of Ag-specific IgG antibodies, activated T cell-biased cytokine responses, mitigated paralytic signs, increased survival rate, and decreased viral amplification, indicating its potential as a safe and effective HFMD vaccine candidate.
BIOMOLECULES & THERAPEUTICS
(2023)
Article
Multidisciplinary Sciences
Liping Wang, Miao Zhu, Yulu Fang, Hao Rong, Liuying Gao, Qi Liao, Lina Zhang, Changzheng Dong
Summary: This study predicted the conformational epitopes of EV-A71 and CV-A16 using a Bioinformatics-based algorithm, and found that their distribution patterns were similar to that of CV-A10, consisting of three sites. The highly overlapping epitopes indicated the reliability of the predictions, suggesting a basic distribution pattern of three sites for enterovirus capsids.
SCIENTIFIC REPORTS
(2021)
Article
Public, Environmental & Occupational Health
Lixia Ye, Jieping Chen, Ting Fang, Rui Ma, Jianmei Wang, Xingqiang Pan, Hongjun Dong, Guozhang Xu
Summary: This study investigated the coverage and timeliness of EV71 vaccination among children born in Ningbo, Zhejiang province from 2012 to 2018. Results showed that there is room for improvement in increasing EV71 vaccination coverage and timeliness, especially in addressing disparities among different populations. The study highlights the importance of simultaneous administration in increasing coverage and timeliness of EV71 vaccination.
Article
Immunology
Jin Luo, Chunling Huo, Huan Qin, Junhong Hu, Lei Lei, Zishu Pan
Summary: This study prepared EV71-VLP and its chimeras, finding that the chimeras provided better cross-protection against CA16 infection compared to EV71-VLP, and induced potent cellular immune responses while decreasing Th2 type cytokine secretion.
Article
Immunology
Jun Gao, Fenyang Tang, Zhiguo Wang, Jing Yu, Ran Hu, Li Liu, Guodong Kang
Summary: This study evaluated the safety profiles of two types of EV71 vaccines administered in Jiangsu Province, China since 2017. A total of 209, 407, and 344 AEFIs cases were reported between 2017 and 2019, with common symptoms including fever, irritability, and allergic eruptions. No significant differences in rare reactions were found between the two types of vaccines.
Article
Microbiology
Rajesh Vikkurthi, Asgar Ansari, Anupama R. Pai, Someshwar Nath Jha, Shilpa Sachan, Suvechchha Pandit, Bhushan Nikam, Anurag Kalia, Bimal Prasad Jit, Hilal Ahmad Parray, Savita Singh, Pallavi Kshetrapal, Nitya Wadhwa, Tripti Shrivastava, Poonam Coshic, Suresh Kumar, Pragya Sharma, Nandini Sharma, Juhi Taneja, Anil K. Pandey, Ashok Sharma, Ramachandran Thiruvengadam, Alba Grifoni, Daniela Weiskopf, Alessandro Sette, Shinjini Bhatnagar, Nimesh Gupta
Summary: The inactivated vaccine BBV152 induces robust and persistent immune memory to both SARS-CoV-2 and its variants. The vaccine generates comparable levels of antibodies to natural infection and provides some protection against variant strains. Additionally, the vaccine activates CD4(+) and CD8(+) T cells and induces follicular T helper cells that assist B cells.
NATURE MICROBIOLOGY
(2022)
Article
Virology
Wei-Ping Jin, Chen Wang, Jie Wu, Jing Guo, Sheng-Li Meng, Ze-Jun Wang, Dai-Guan Yu, Shuo Shen
Summary: This study focuses on the recombinants of Coxsackievirus A5 (CV-A5) carrying reporter genes of different lengths, which have been proven to efficiently express in vitro. These recombinants can be used to establish a rapid and convenient neutralizing antibody assay and study the infection and mechanisms of CV-A5 pathogenicity in a mouse model.
Article
Virology
Andrew R. Swartz, Yvonne Shieh, Amanda Gulasarian, Jessica W. Olson, Richard R. Rustandi
Summary: A purification method using affinity chromatography has been developed for extracting CVA21 virus from cell culture, with the binding and recovery of the virus depending on different cell culture conditions.
Article
Immunology
Hongchao Jiang, Zhen Zhang, Qing Rao, Xiaodan Wang, Meifen Wang, Tingyi Du, Jiaolian Tang, Shuying Long, Juan Zhang, Jia Luo, Yue Pan, Junying Chen, Jing Ma, Xiaomei Liu, Mao Fan, Tiesong Zhang, Qiangming Sun
Summary: The use of EV-A71 inactivated vaccine in Kunming City significantly reduced the incidence of EV-A71, while increasing the proportion of non-CV-A16/EV-A71 EVs infections. CV-A6 was identified as the most important causative agent in all clinical symptoms, highlighting the need for developing 4-valent combined vaccines to provide broader protection.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Infectious Diseases
Lifen Zhang, Fan Gao, Gang Zeng, Haitao Yang, Taotao Zhu, Shuangmin Yang, Xing Meng, Qunying Mao, Xiaoqiang Liu
Summary: The study demonstrated good safety and immunogenicity of the Sinovac EV71 vaccine in children aged 36-71 months, showing superior results compared to the control group.
JOURNAL OF THE PEDIATRIC INFECTIOUS DISEASES SOCIETY
(2021)
Article
Surgery
Li Lei, Qing Li, Shuhong Xu, Mingyang Tian, Xinghui Zheng, Yunxia Bi, Bo Huang
Summary: Three VP1 peptides with high immunogenicity were selected for vaccine injection, showing improved inflammatory response and reduced virus levels in EV71-infected mice. The injection also decreased lesions in the small intestines of infected mice and protected brain tissues from the infection.
ANNALS OF TRANSPLANTATION
(2021)
Article
Immunology
Xiaodong Liu, Shaoying Chang, Ruize Wang, Yanhui Xiao, Fangjun Li, Qing Xu, Shaobai Zhang, Xiao Chen, Shangxiao Zhang, Min Zhang, Xiaoqi Chen, Qingfan Cao, Xiaoyu Liu, Hui Wang, Daihong Zhan, Haiping Chen, Wei Chen, Jianyong Jiang, Chao Zhang, Haijiao Wang, Lidong Gao, Xuanwen Shi, Xiaoming Yang, Aiqiang Xu
Summary: This study investigated the immunogenicity and safety of the enterovirus 71 vaccine. The results showed that simultaneous administration of the vaccine with other vaccines was as effective and safe as separate administration.
Article
Immunology
Zhi-qun Li, Zi-qi Qin, Hui-feng Tan, Chun-huan Zhang, Jian-xiong Xu, Jian Chen, Li-Hong Ni, Xue-xia Yun, Min Cui, Yong Huang, Wen Wang, Zhou-bin Zhang
Summary: The study found that the EV71 vaccine coverage rate is low in Guangzhou, but the vaccines from the three manufacturers have good safety with no significant differences in AEFI responses. It is recommended to strengthen vaccine publicity and increase vaccine coverage rate in children.
EXPERT REVIEW OF VACCINES
(2021)
Article
Pharmacology & Pharmacy
Wen-Chun Liu, Ying-Yu Liu, Ting-Hsuan Chen, Chia-Chyi Liu, Jia-Tsrong Jan, Suh-Chin Wu
ANTIVIRAL RESEARCH
(2016)
Article
Pharmacology & Pharmacy
Chia-Chyi Liu, Meng-Shin Guo, Shang-Rung Wu, Hsiao-Yu Lin, Ya-Ting Yang, Wei-Chih Liu, Yen-Hung Chow, Dar-Bin Shieh, Jen-Ren Wang, Pele Chong
ANTIVIRAL RESEARCH
(2016)
Article
Pharmacology & Pharmacy
Hsiao-Yun Shao, Huai-Sheng Hsu, Shu-Ling Yu, Shang-Rung Wu, Kai-Chieh Hu, Ching-Kun Chang, Chia-Chyi Liu, Yen-Hung Chow
ANTIVIRAL RESEARCH
(2016)
Article
Immunology
Chia-Chyi Liu, Suh-Chin Wu, Shang-Rung Wu, Hsiao-Yu Lin, Meng-Shin Guo, Alan Yung-Chih Hu, Yen-Hung Chow, Jen-Ron Chiang, Dar-Bin Shieh, Pele Chong
Article
Virology
Hsiao-Yun Shao, Ying-Chin Chen, Nai-Hsiang Chung, Yi-Ju Lu, Ching-Kun Chang, Shu-Ling Yu, Chia-Chyi Liu, Yen-Hung Chow
Correction
Multidisciplinary Sciences
Ching-Kun Chang, Shang-Rung Wu, Ying-Chin Chen, Kuen-Jin Lee, Nai-Hsiang Chung, Yi-Ju Lu, Shu-Ling Yu, Chia-Chyi Liu, Yen-Hung Chow
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Ching-Kun Chang, Shang-Rung Wu, Ying-Chin Chen, Kuen-Jin Lee, Nai-Hsiang Chung, Yi-Ju Lu, Shu-Ling Yu, Chia-Chyi Liu, Yen-Hung Chow
SCIENTIFIC REPORTS
(2018)
Article
Biotechnology & Applied Microbiology
Ting-Hsuan Chen, Wen-Chun Liu, Chia-Ying Lin, Chia-Chyi Liu, Jia-Tsrong Jan, Maureen Spearman, Michael Butler, Suh-Chin Wu
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Review
Immunology
Chih-Yeu Fang, Chia-Chyi Liu
EXPERT REVIEW OF VACCINES
(2018)
Article
Pharmacology & Pharmacy
Jo-Yu Hong, Ting-Hsuan Chen, Yu-Jou Chen, Chia-Chyi Liu, Jia-Tsrong Jan, Suh-Chin Wu
ANTIVIRAL RESEARCH
(2019)
Article
Immunology
Ting-Hsuan Chen, Wen-Chun Liu, I-Chen Chen, Chia-Chyi Liu, Ming-Hsi Huang, Jia-Tsrong Jan, Suh-Chin Wu
Review
Pharmacology & Pharmacy
Chih-Yeu Fang, Chia-Chyi Liu
Summary: Hand, foot, and mouth disease poses a serious threat to young children in the Asia-Pacific region, with enterovirus A71 (EV-A71) being a major cause of severe complications. While vaccines exist to prevent EV-A71 infection, there is a lack of effective treatments for other enteroviruses. Novel strategies are needed to develop new therapies for emerging enteroviruses associated with HFMD, including the use of DNA/RNA vaccine technology, structure biology, neutralization antibody studies, and nucleotide aptamer library screening.
EXPERT OPINION ON DRUG DISCOVERY
(2022)
Article
Pharmacology & Pharmacy
Chi-Hsun Chen, Chung-Chu Chen, Wei-Bo Wang, Vania Lionel, Chia-Chyi Liu, Li-Min Huang, Suh-Chin Wu
Summary: In this study, a recombinant ZIKV envelope DIII protein genetically fused with Salmonella typhimurium flagellin was developed as a mucosal antigen. Intranasal immunization with this antigen elicited strong protective immunity against ZIKV challenges, demonstrating its potential for developing mucosal vaccines.
Article
Biology
Seii Ohka, Soon Hao Tan, Eri Ishiyama, Katsutoshi Ogasawara, Tomohito Hanasaka, Kinji Ishida, Kyoji Hagiwara, Chia-Chyi Liu, Pele Choi-Sing Chong, Ken-ichi Hanaki, Giampietro Schiavo
Summary: Recent research has shown that EV71, a causative agent of hand-foot-and-mouth disease, undergoes efficient uncoating in late endosomes containing hSCARB2, M6PR, RAB9, bis(monoacylglycero)phosphate, and LAMP2. The autophagic pathway does not play a critical role in EV71 uncoating, and cation-dependent M6PR is likely to be important for this process.
Article
Biochemical Research Methods
Sheng-Chieh Lien, Chia-Chun Lu, Yu-Sheng Shen, Ya-Ting Yang, Shang-Rung Wu, Chih-Yeu Fang, Yen -Hung Chow, Ching -Len Liao, Jen -Ron Chiang, Chia-Chyi Liu
Summary: This study aimed to develop an approach to isolate and purify highly immunogenic mature EV-A71 particles. By applying a step gradient elution procedure, VP0-cleaved particles of EV-A71 were successfully isolated using Q-membrane anion-exchange chromatography. The purified VP0-cleaved particles exhibited characteristics equivalent to highly infectious F-particles of EV-A71.
JOURNAL OF CHROMATOGRAPHY A
(2022)
