Article
Chemistry, Medicinal
Jiaxiang Gu, Bin Wang, Tianliang Wang, Naichen Zhang, Hongjun Liu, Jianchao Gui, Yiming Lu
Summary: In this study, the effects of cartilage progenitor cells (CPCs), bone marrow mesenchymal stem cells (BMSCs), and chondrocytes (CCs) on cartilage repair were investigated. The results showed that CPCs had higher proliferation ability compared to CCs and BMSCs. Moreover, CPCs seeded in agarose gel exhibited significantly higher levels of collagen, glycosaminoglycan, DNA content, and chondrocyte-related gene expression. Integration strength in the CPCs seeded gel was also higher than the other two gels. These findings suggest that CPCs have advantages over CCs and BMSCs as seed cells in tissue engineering for cartilage repair.
DRUG DESIGN DEVELOPMENT AND THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Ke Xue, Shuqi Zhang, Jin Ge, Qiang Wang, Lin Qi, Kai Liu
Summary: This study compares the effects of adding Bioglass into PHBV scaffolds on the characteristics of in vivo tissue-engineered cartilages. The results show that the PHBV/10% Bioglass scaffold has better hydrophilicity, cell adhesion, cartilage formation, and biomechanical performance than the pure PHBV scaffold.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Chong Zhang, Guanhuier Wang, Yang An
Summary: This review discusses the new strategy of in situ regeneration of nasal septal cartilage through scaffold-based tissue engineering. By summarizing the characteristics and migratory ability of nasal septal CPCs, as well as their roles in cartilage regeneration, strategies to promote CPCs migration and chondrogenesis are proposed, providing ideas for achieving nasal septal cartilage in situ regeneration.
Article
Biotechnology & Applied Microbiology
Jingsong Wang, Sally Roberts, Weiping Li, Karina Wright
Summary: This study identifies and characterizes progenitor cell populations in different regions of the human meniscus. Progenitor cells from the vascular region show enhanced proliferative and fibrochondrogenic characteristics compared to those from the avascular region. These findings indicate that meniscal progenitors may be a promising cell therapy strategy for meniscal regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Kai Feng, Yifan Yu, Zhengsheng Chen, Feng Wang, Kunqi Zhang, Hongfang Chen, Jia Xu, Qinglin Kang
Summary: Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage destruction. Cartilage progenitor cells (CPCs)-based therapy has shown promise in repairing degenerated articular cartilage. In this study, hypoxia-preconditioned 3D cultured CPCs-laden GelMA microspheres (CGMs) were constructed and characterized, showing enhanced cartilage extracellular matrix (ECM) secretion and chondrogenic ability. These hypoxia-preconditioned CPCs effectively maintained cartilage matrix metabolism balance and attenuated articular cartilage degeneration in OA models. Mechanistically, they exerted chondroprotective effects by inhibiting inflammation and oxidative stress via the NRF2/HO-1 pathway.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Marija Mazor, Eric Lespessailles, Thomas M. Best, Mazen Ali, Hechmi Toumi
Summary: Recent research has shown that cells isolated from osteoarthritic cartilage have the potential to form hyaline-like cartilage tissue. In this study, the researchers examined the marker expression and chondrogenetic potential of cells from mild, moderate, and severe osteoarthritic tissue. They found that only cells from moderate osteoarthritis were able to form hyaline cartilage-like tissue, while cells from severe and mild osteoarthritis did not. These findings have implications for understanding the repair capacity of articular cartilage in osteoarthritis and suggest that these progenitor cells may be a candidate for cartilage repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Florian Helms, Skadi Lau, Thomas Aper, Sarah Zippusch, Melanie Klingenberg, Axel Haverich, Mathias Wilhelmi, Ulrike Boeer
Summary: Physiologically organized three-layered bioartificial vessels were successfully manufactured by stepwise fibrin molding with subsequent mechanical stimulation, demonstrating longitudinal alignment of luminal endothelial cells, circumferential alignment of cells in the media-layer expressing SMC marker proteins, and longitudinally aligned microvascular tubes in the adventitia-layer.
ANNALS OF BIOMEDICAL ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Alexandre Dufour, Frederic Mallein-Gerin, Emeline Perrier-Groult
Summary: In this study, a combination of soluble factors, fibrin hydrogel, direct perfusion, and human articular chondrocytes was used to engineer large cartilage tissues. Perfusion preconditioning improved the quality and spatial distribution of the cartilage matrix, resulting in the reconstruction of hyaline cartilage and increased production of the main component of the pericellular matrix by chondrocytes.
APPLIED SCIENCES-BASEL
(2021)
Article
Medicine, Research & Experimental
Ngoc-Trinh Tran, Minh-Dung Truong, Hee-Woong Yun, Byoung-Hyun Min
Summary: This study aims to analyze and evaluate the potential of human fetal cartilage progenitor cells-secretome (ShFCPC) in modifying osteoarthritis (OA) process. The secreted proteins enriched in ShFCPC were characterized and shown to possess biological functions that protect chondrocytes, suppress inflammation, and promote cartilage repair. These findings suggest that ShFCPC may be a novel therapeutic agent for OA.
Article
Engineering, Biomedical
Mahboubeh Sadat Mousavi, Ghassem Amoabediny, Seyed Hossein Mahfouzi, Seyed Hamid Safiabadi Tali
Summary: The study introduced a novel perfusion-based bioreactor (PBB) method for efficient decellularization of bovine articular cartilages, resulting in better preservation of the tissue structure and essential components while reducing harmful physical and chemical steps and process duration. The PBB method showed a 90% reduction in DNA content, significantly higher than the control method, and maintained around 50% of sulfated glycosaminoglycan (sGAG) content and 92% of compression properties, demonstrating efficient preservation of scaffold structure and components. Additionally, the PBB specimens supported cell adherence and proliferation effectively, showing promise for producing close-to-natural scaffolds for cartilage tissue engineering.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Yuki Fujisawa, Tomoka Takao, Daisuke Yamada, Takeshi Takarada
Summary: In this study, we successfully generated a large amount of hiPSC-derived cartilaginous particles using tissue engineering techniques, showing characteristics of hyaline cartilage, and this method has the potential for scaling up cartilage regeneration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Salomi Desai, Mark Dooner, Jake Newberry, John Twomey-Kozak, Janine Molino, Jay Trivedi, Jay M. Patel, Brett D. Owens, Chathuraka T. Jayasuriya
Summary: In a rodent model, treatment with stable cartilage-derived progenitor cell lines has been shown to stimulate meniscus tear healing and provide chondroprotection.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Jianhua Zhang, Esther Wehrle, Marina Rubert, Ralph Mueller
Summary: Three-dimensional (3D) bioprinting is a promising approach for tissue engineering, allowing precise placement of cells and biomaterials to mimic complex microstructures of biological tissues. Bioprinted tissues can benefit from exposure to fluid mechanical cues provided by bioreactor systems, aiding in tissue development and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Paper & Wood
Roya Salehi, Leila Mohammadzadeh, Mehrdad Mahkam, Abbas Jafarizad, Reza Rahbarghazi
Summary: This study evaluated the effects of PCL/SF/SESM/Gel and PCL/SF/SESM/MC hybrid scaffolds on skin progenitor cells. The results showed that the addition of Gel and MC increased the diameter size and surface wettability of the nanofibers, improved cell viability, and promoted the differentiation of skin cells into keratinocyte-like cells.
Article
Biochemistry & Molecular Biology
Maria Oliver-Ferrandiz, Lara Milian, Maria Sancho-Tello, Jose Javier Martin de Llano, Fernando Gisbert Roca, Cristina Martinez-Ramos, Carmen Carda, Manuel Mata
Summary: A novel MACI scaffold composed of a mixed alginate-agarose hydrogel with human dental pulp stem cells (hDPSCs) was proposed, showing superior biomechanical properties and uniform cell distribution. The hDPSCs cultured in the scaffold exhibited good chondrogenic differentiation potential.
Article
Orthopedics
Yuji Kohno, Hideyuki Koga, Nobutake Ozeki, Junpei Matsuda, Mitsuru Mizuno, Hisako Katano, Ichiro Sekiya
Summary: The study investigated the effect of centralization with modifications on meniscus biomechanics, showing that centralization procedures can effectively redistribute loads on the tibial cartilage and meniscus. Centralization with capsular advancement was found to be the most effective treatment for an extruded meniscus after partial meniscectomy in terms of reducing tibial cartilage load.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2022)
Article
Orthopedics
Ichiro Sekiya, Hideyuki Koga, Hisako Katano, Mitsuru Mizuno, Yuji Kohno, Koji Otabe, Nobutake Ozeki
Summary: The arthroscopic findings of degenerative flap and radial tear of the medial meniscus were improved one year after treatment with meniscus repair and synovial mesenchymal stem cell transplantation.
JOURNAL OF ORTHOPAEDIC SCIENCE
(2022)
Article
Cell & Tissue Engineering
Misaki Yagi, Mitsuru Mizuno, Ryota Fujisawa, Hisako Katano, Kentaro Endo, Nobutake Ozeki, Yuriko Sakamaki, Hideyuki Koga, Ichiro Sekiya
Summary: This study investigated the optimal pore size of honeycomb PLA films for in vitro cartilage formation using synovial MSCs. The results showed that the 5 μm pore size was more suitable for cartilage formation compared to the 20 μm pore size.
STEM CELLS INTERNATIONAL
(2021)
Article
Orthopedics
Nobutake Ozeki, Yusuke Nakagawa, Mitsuru Mizuno, Yuji Kohno, Hisako Katano, Hideyuki Koga, Ichiro Sekiya
Summary: The study developed an ultrasound-guided harvesting procedure for synovium for regenerative medicine of cartilage and meniscus, which is minimally invasive and may pose a risk for hemostasis.
ARTHROSCOPY TECHNIQUES
(2021)
Article
Anatomy & Morphology
Yuji Kohno, Mitsuru Mizuno, Kentaro Endo, Nobutake Ozeki, Hisako Katano, Mikio Matsumoto, Haruka Kaneko, Yuji Takazawa, Hideyuki Koga, Ichiro Sekiya
Summary: This study aimed to determine whether the yield of synovial fluid MSCs could predict the ultimate yield of primary MSCs from the synovium of patients with RA. The results showed a positive correlation between the number of synovial fluid cells and synovial cells, and the synovial fluid MSCs exhibited MSC-like characteristics and multi-differentiation potential. These findings reveal the feasibility of using synovial fluid MSC yield to predict the synovial MSC yield.
Article
Cell & Tissue Engineering
Mitsuru Mizuno, Takahisa Matsuzaki, Nobutake Ozeki, Hisako Katano, Hideyuki Koga, Takanori Takebe, Hiroshi Y. Yoshikawa, Ichiro Sekiya
Summary: This study compared the freeze-thaw tolerance of synovial mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVECs), and identified the cell membrane fluidity and antioxidant capacity as important factors contributing to the differences. These findings have implications for analyzing cell biology and membrane physics, and establishing appropriate long-term preservation methods to promote transplantation therapies.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Takahiro Tanimoto, Kentaro Endo, Yuriko Sakamaki, Nobutake Ozeki, Hisako Katano, Mitsuru Mizuno, Hideyuki Koga, Ichiro Sekiya
Summary: This study confirms the adhesion of synovial stem cells to degenerated cartilage and shows that the adhesion increases over time. The study also observes morphological changes in the stem cells during the adhesion process, transitioning from microspikes to pseudopodia.
SCIENTIFIC REPORTS
(2022)
Article
Cell & Tissue Engineering
Mitsuru Mizuno, Yoshimi Sugahara, Daisuke Iwayama, Noe Miyashita, Hisako Katano, Ichiro Sekiya
Summary: This study aims to investigate the attributes, stress factors, and motivational factors of cell processing operators (CPOs). The results showed that more than half of the CPOs had less than two years of work experience and were more likely to encounter various stressful situations compared to CPOs with three or more years of experience. In particular, wearing dust-free clothing and working in a unique environment like a clean room, where breaks are difficult due to the closed space nature, were considered particularly stressful situations. However, these CPOs had high motivations based on their pride in participating in cutting-edge medical care and contributing to patient care.
REGENERATIVE THERAPY
(2022)
Review
Cell & Tissue Engineering
Mitsuru Mizuno, Nobutake Ozeki, Ichiro Sekiya
Summary: Cell therapy is a promising alternative treatment option for osteoarthritis, but the safety of using expanded mesenchymal stem cells (MSCs) for transplantation needs further assessment. Karyotypic abnormalities, including chromosomal mutations, may occur during the cell-expansion process. The suitability of cells with these abnormalities for cell therapy has not been evaluated.
REGENERATIVE THERAPY
(2022)
Article
Cell Biology
Shunichi Fujii, Kentaro Endo, Nobutake Ozeki, Yuriko Sakamaki, Yuji Kohno, Mitsuru Mizuno, Hisako Katano, Kunikazu Tsuji, Hideyuki Koga, Ichiro Sekiya
Summary: Thawed MSCs adhere better than cultured MSCs immediately after placement, but adhesion is similar after 10 min. The presence of microspikes is associated with better adhesion of synovial MSCs immediately after placement.
BMC MOLECULAR AND CELL BIOLOGY
(2022)
Article
Immunology
Nobutake Ozeki, Mitsuru Mizuno, Shinobu Yanada, Takuya Okada, Rei Kubota, Yoshihisa Kushida, Hideto Furuoka, Kentaro Endo, Hisako Katano, Kentaro Nakamura, Shoji Ohya, Hideyuki Koga, Ichiro Sekiya
Summary: The study investigated the effect of autologous synovial mesenchymal stem cell transplantation on meniscus healing. The results showed that autologous MSC transplantation can suppress inflammation caused by synovial harvesting and promote meniscus repair.
TRANSPLANTATION PROCEEDINGS
(2023)
Article
Cell & Tissue Engineering
Mitsuru Mizuno, Junpei Matsuda, Ken Watanabe, Norio Shimizu, Ichiro Sekiya
Summary: The risk of cross-contamination during the process of cell product changeover is high. This study evaluated the effectiveness of different disinfectants and manual wiping methods in removing bacteria during cell processing.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Mitsuru Mizuno, Kouichirou Yori, Toshikazu Takeuchi, Tetsuya Yamaguchi, Ken Watanabe, Yasuhiro Tomaru, Norio Shimizu, Ichiro Sekiya
Summary: Minimizing cross-contamination risks is crucial during cell-product processing. Human error increases contamination risk in biosafety cabinets. UV irradiation effectively eliminates cross-contamination risks, and monitoring the UV dose in the biosafety cabinet is a promising method for reducing cross-contamination.
REGENERATIVE THERAPY
(2023)
Article
Cell & Tissue Engineering
Mitsuru Mizuno, Koki Abe, Takashi Kakimoto, Keiichi Yano, Yukiko Ota, Kengo Tomita, Naoki Kagi, Ichiro Sekiya
Summary: The study aimed to evaluate the air quality in cell processing facilities (CPF) and detect volatile organic compounds (VOCs) from disinfectants and building materials, as well as airborne ionic substances from outdoor air. The results showed that CPFs had relatively lower levels of chloride ions, nitrogen oxides, and sulfur oxides compared to outdoor air. Ethanol and toluene were the main VOCs detected. The study suggests that the health risks to cell processing operators may increase with the expansion of cell processing.
REGENERATIVE THERAPY
(2023)
Article
Multidisciplinary Sciences
Hisako Katano, Nobutake Ozeki, Mitsuru Mizuno, Kentaro Endo, Noriya Okanouchi, Jo Fujita, Jun Masumoto, Hideyuki Koga, Ichiro Sekiya
Summary: By observing three-dimensional magnetic resonance images from a cohort population, we clarified the frequency, localization, and morphological progression of patellofemoral osteoarthritis. The prevalence of patellar cartilage defects was higher in the medial than lateral regions, and the defects were divided into confined and unconfined types.
SCIENTIFIC REPORTS
(2023)
