Article
Biotechnology & Applied Microbiology
Guoshuang Zheng, Chundong Xue, Fang Cao, Minghui Hu, Maoyuan Li, Hui Xie, Weiting Yu, Dewei Zhao
Summary: In this study, an alginate/collagen (ALG/COL) hybrid hydrogel with different guluronate/mannuronate acid ratios was developed. The mechanical stiffness of the hydrogel was significantly affected by the G/M ratios of alginate. Chondrocytes cultured on the Mid-G/M hydrogels exhibited better viability and phenotype preservation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Suyoung Been, Jeongmin Choi, Hunhwi Cho, Gayeong Jeon, Jeong E. Song, Alessio Bucciarelli, Gilson Khang
Summary: The study demonstrated that the agarose/eggshell membrane scaffold can be used for chondrocyte transplantation. This scaffold has excellent biocompatibility and biodegradability, suitable for forming a hydrogel with three-dimensional porosity.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Polymer Science
Miguel-Angel Martin-Piedra, Belen Girones-Camarasa, Antonio Espana-Lopez, Ricardo Fernandez-Valades Gamez, Cristina Blanco-Elices, Ingrid Garzon, Miguel Alaminos, Ricardo Fernandez-Valades
Summary: The use of cellular bone substitutes showed improvements in maxillofacial structures but did not fully succeed in generating dense bone tissue at the regeneration area. Preliminary signs suggest potential usefulness of cellular fibrin-agarose bone substitutes in severe critical mandibular bone defects.
Article
Orthopedics
David Grevenstein, Andreas Mamilos, Volker H. Schmitt, Tanja Niedermair, Willi Wagner, C. James Kirkpatrick, Christoph Brochhausen
Summary: The study analyzed histological and immunohistological findings from five patients who underwent spheroid-based autologous chondrocyte transplantation after traumatic lesions of the knee, showing excellent histological results in terms of hyaline articular cartilage regeneration. This indicates that spheroid-based autologous chondrocyte transplantation is a promising strategy for treating traumatic lesions of the knee's articular cartilage.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Po-Chih Shen, Cheng-Chang Lu, Shih-Hsiang Chou, Zi-Miao Liu, Shu-Jem Su, Yin-Chun Tien
Summary: This study compared stratified chondrocyte sheets (S-CS) and heterogeneous chondrocyte sheets (H-CS) in terms of expression and therapeutic effects, revealing that the S-CS group exhibited higher cell viability, proliferation rate, expression of chondrogenic markers, secretion of tissue inhibitor of metalloproteinase, and GAG production level. The mini-pigs articular cartilage defect model showed that the S-CS group had a higher International Cartilage Repair Society (ICRS) macroscopic score and displayed a zonal structure closer to native cartilage.
Article
Engineering, Biomedical
Sheng-Long Ding, Xin Liu, Xi-Yuan Zhao, Ke-Tao Wang, Wei Xiong, Zi-Li Gao, Cheng-Yi Sun, Min-Xuan Jia, Cheng Li, Qi Gu, Ming-Zhu Zhang
Summary: The successful regeneration of cartilage tissue at a clinical scale has been a significant challenge. Microcarrier systems offer an attractive approach to regulate cell phenotype and tissue maturation, and have shown promise in cartilage regeneration. This review provides comprehensive guidelines for the rational design and applications of microcarriers in cartilage tissue engineering.
BIOACTIVE MATERIALS
(2022)
Review
Chemistry, Applied
Sumit Murab, Aastha Gupta, Malgorzata Katarzyna Wlodarczyk-Biegun, Anuj Kumar, Patrick van Rijn, Patrick Whitlock, Sung Soo Han, Garima Agrawal
Summary: This review highlights the importance of alginate-based hydrogel inks for fabricating 3D printed scaffolds for bone and cartilage regeneration. The fundamentals of direct extrusion 3D bioprinting method are discussed, and a comprehensive overview of various alginate-based hydrogel ink formulations that have been used so far is provided. The requirements of hydrogel inks and 3D printed scaffolds to achieve similarity to the native tissue environment are summarized.
CARBOHYDRATE POLYMERS
(2022)
Article
Engineering, Biomedical
Yun Chu, Lei Huang, Wangping Hao, Tongtong Zhao, Haitao Zhao, Wen Yang, Xin Xie, Lin Qian, Yanyan Chen, Jianwu Dai
Summary: 3D bioprinting of double crosslinked alginate hydrogel promotes MSC differentiation into cartilage and demonstrates good mechanical properties, stability, and printing accuracy.
BIOMEDICAL MATERIALS
(2021)
Article
Orthopedics
Yuxin Yan, Rao Fu, Chuanqi Liu, Jing Yang, Qingfeng Li, Ru-Lin Huang
Summary: A sequential chondrocyte isolation protocol was developed with higher cell-yield efficiency and better chondrocyte quality compared to the classic protocol. Cells harvested after sequential digestions demonstrated higher viability, proliferative activity, chondrocyte phenotype, and cartilage-specific matrix synthesis ability.
Article
Biochemistry & Molecular Biology
Xavier Barcelo, Kian F. Eichholz, Orquidea Garcia, Daniel J. Kelly
Summary: The study investigated the effect of alginate oxidation on the degradation rate of bioinks, resulting in a range of degradable bioinks with the degradation rate highly dependent on the content of partially oxidized alginate. Despite high mass loss, all bioink blends except the 0:100 group maintained the initially printed geometry throughout a 4 week in vitro culture period and supported robust chondrogenic differentiation of mesenchymal stem/stromal cells.
Article
Biochemistry & Molecular Biology
Panneerselvam Jithendra, Jamal Moideen Muthu Mohamed, Dinesh Annamalai, Rasha Hamed Al-Serwi, Ateya Megahed Ibrahim, Mohamed El-Sherbiny, Abraham Merlin Rajam, Mamdouh Eldesoqui, Nimer Mansour
Summary: The chondrogenic efficacy of aloe vera blended collagen-chitosan (COL-CS-AV) porous scaffold was investigated using articular chondrocytes. The scaffold showed higher chondrogenic efficacy compared to COL-CS, making it a suitable candidate for cartilage tissue engineering. The study utilized various assays and staining techniques to analyze cytocompatibility, GAG content, RNA content, and expression of cartilage-specific genes and collagen type II.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Polymer Science
Maciej Plonczak, Monika Wasyleczko, Tomasz Jakutowicz, Andrzej Chwojnowski, Jaroslaw Czubak
Summary: This study presents Autologous Chondrocyte Implantation (ACI) on two different scaffolds, Chondro-Gide made of collagen type I/III and a polyethersulfone (PES) synthetic membrane, for the treatment of lesions in hyaline cartilage in rabbits. The use of PES membranes with desirable features for chondrocyte cultivation is the revolutionary idea in this study.
Article
Chemistry, Multidisciplinary
Sheng-Long Ding, Xi-Yuan Zhao, Wei Xiong, Lin-Feng Ji, Min-Xuan Jia, Yan-Yan Liu, Hai-Tao Guo, Feng Qu, Wenguo Cui, Qi Gu, Ming-Zhu Zhang
Summary: Cartilage equivalents from hydrogels containing chondrocytes show great potential in hyaline cartilage regeneration. However, current methods struggle to recreate the original architecture and culture non-differentiated chondrocytes in vitro. This study introduces specially designed microcarriers that use mechanotransductive conditions to form stable hyaluronic acid, contributing to the regeneration of hyaline cartilaginous microtissue and preventing chondrocyte dedifferentiation.
ADVANCED MATERIALS
(2023)
Article
Polymer Science
Mike Wagenbrenner, Susanne Mayer-Wagner, Maximilian Rudert, Boris Michael Holzapfel, Manuel Weissenberger
Summary: Cartilage has limited regenerative capacity, and cell-based approaches are a promising alternative for the treatment of cartilage defects and osteoarthritis, with MSCs being an attractive cell source often combined with hydrogels. This review focused on the advantages and disadvantages of various hydrogels, as well as different combinations of MSC subpopulations and hydrogels used in cartilage engineering.
Article
Engineering, Biomedical
Zhonghan Wang, Hanxiang Le, Yanbing Wang, He Liu, Zuhao Li, Xiaoyu Yang, Chenyu Wang, Jianxun Ding, Xuesi Chen
Summary: The development of interdisciplinary biomedical engineering has made significant breakthroughs in the field of cartilage regeneration. However, cartilage defects are more complex under clinical conditions, especially when injuries occur at specific sites or under inflammatory microenvironments. This review summarizes the strategies for cartilage regeneration at particular sites and discusses the challenges and opportunities for clinical transformation.
BIOACTIVE MATERIALS
(2022)
Article
Cell & Tissue Engineering
Sara Martin-Iglesias, Lara Milian, Maria Sancho-Tello, Ruben Salvador-Clavell, Jose Javier Martin de Llano, Carmen Carda, Manuel Mata
Summary: Bone tissue engineering provides an alternative approach for bone regeneration therapies. In this study, a model for in vitro osteogenic differentiation based on mesenchymal stem cells (MSCs) was developed to analyze the influence of culture media and cell origin on the efficiency of the process. The results showed that the osteogenic marker levels were influenced by the culture medium and the cell origin used, with higher levels observed when hADSCs were cultured with medium supplemented with BMP-2. Additionally, the 3D culture environment was suitable for cell survival and osteogenic induction.
STEM CELLS INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Fernando Gisbert Roca, Luis Gil Santos, Manuel Mata Roig, Lara Milian Medina, Cristina Martinez-Ramos, Manuel Monleon Pradas
Summary: This study presents a novel multimodular nerve guidance conduit for the treatment of large nerve damages. The conduit consists of aligned PLA microfibers and HA conduits that retain pre-seeded auxiliary cells. The results demonstrate that this multimodular approach is effective for promoting nerve regeneration and provides new surgical solutions in this field.
Article
Biochemistry & Molecular Biology
Lara Milian, Irene Monleon-Guinot, Maria Sancho-Tello, Jose Marcelo Galbis, Antonio Cremades, Maria Almenar-Ordaz, Josep Penaroja-Martinez, Rosa Farras, Jose Javier Martin de Llano, Carmen Carda, Manuel Mata
Summary: Evidence suggests that cannabinoid agonists inhibit relevant aspects of lung cancer, such as proliferation and epithelial-to-mesenchymal transition (EMT). The effects of tetrahydrocannabinol (THC) and cannabidiol (CBD) on lung cancer-associated fibroblasts (CAFs) and normal fibroblasts (NFs) were evaluated in this study. The results demonstrate the potential of cannabinoid agonists for the treatment of lung cancer and their effects on CAFs in relation to cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Fernando Gisbert Roca, Sara Serrano Requena, Manuel Monleon Pradas, Cristina Martinez-Ramos
Summary: In this study, an electroconductive fibrillar substrate was used to guide axonal growth and accelerate its growth rate. The substrate showed the ability to guide axonal growth in a highly aligned manner and the growth was further improved with electrical stimulation. The upregulation of genes related to axon guidance, axogenesis, Schwann cells, proliferation and neurotrophins was also observed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Irene Monleon-Guinot, Lara Milian, Patricia Martinez-Vallejo, Maria Sancho-Tello, Mauro Llop-Miguel, Jose Marcelo Galbis, Antonio Cremades, Carmen Carda, Manuel Mata
Summary: The malignancy of lung cancer is influenced by the tumor microenvironment, particularly the presence of cancer-associated fibroblasts (CAFs). This study generated organoids by combining A549 cells with CAFs and normal fibroblasts (NF) isolated from adenocarcinoma tumors, and optimized the manufacturing conditions. The addition of stromal cells induced self-organization of the organoids, affected their morphology and growth, and influenced the expression of genes related to epithelial mesenchymal transition (EMT). The results highlight the role of CAFs in lung tumor progression and provide a potential in vitro pharmacological model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
K. Marquez-Florez, D. A. Garzon-Alvarado, C. Carda, M. Sancho-Tello
Summary: Computational models are useful for explaining phenomena that cannot be observed through animal models and have developed a simulation tool to determine the mechanical conditions provided by polymeric scaffolds. The computational model considers the articular cartilage, subchondral bone, and scaffold, modeling them as poroelastic materials with linear-elastic collagen fibers in the cartilage. This model explains the remodeling of subchondral bone and how scaffolds facilitate cartilage regeneration, suggesting their potential to improve tissue growth.
JOURNAL OF THEORETICAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Nestor J. Martinez-Hernandez, Lara Milian-Medina, Jorge Mas-Estelles, Jose L. Monroy-Anton, Jose L. Lopez-Villalobos, David Hervas-Marin, Amparo Roig-Bataller, Manuel Mata-Roig
Summary: This study investigates the effect of an escalated radiation dose on decellularized rabbit trachea. It determines that a dose of 1 kGy achieves complete sterility without significant histological and biomechanical changes in the organ.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Biochemistry & Molecular Biology
Lara Milian, Pilar Molina, Maria Oliver-Ferrandiz, Carlos Fernandez-Sellers, Ana Monzo, Rafael Sanchez-Sanchez, Aitana Braza-Boils, Manuel Mata, Esther Zorio
Summary: This study found the feasibility of isolating and characterizing adipose-derived stem cells (ADSCs) from cadaveric samples, and confirmed their ability to differentiate into chondrocytes and osteocytes. This discovery provides potential applications in tissue engineering and research fields.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Sara Fontana, Laura Caramazza, Paolo Marracino, Irene Cuenca Ortola, Micol Colella, Noemi Dolciotti, Alessandra Paffi, Fernando Gisbert Roca, Sergiy Ivashchenko, Jorge Mas Estelles, Claudia Consales, Marco Balucani, Francesca Apollonio, Micaela Liberti
Summary: This paper presents a numerical study on the effect of microfibrils' scaffolds on E-field distribution in planar interdigitated electrodes. The results show that microfibrils can redistribute the E-field, increasing the maximum value up to 100 kV/m and providing adequate cell exposure.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Fernando Gisbert Roca, Cristina Martinez-Ramos, Sergiy Ivashchenko, Abel Garcia-Bernabe, Vicente Compan, Manuel Monleon Pradas
Summary: Electroconductive materials based on poly(lactic acid) electrospinning membranes grafted with carboxylic group functionalized carbon nanotubes (CNTs) were obtained. The PLA membranes were oxidized with sulfuric acid and subsequently grafted with CNTs, resulting in improved mechanical and conductive properties. The uniform distribution of CNTs on the PLA nanofiber surface and the elimination of the insulating barrier of PLA contributed to the enhanced functionality of the PLA-CNTs membranes for medical and industrial applications.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Engineering, Biomedical
Maria Gomez-alvarez, Clara Bueno-Fernandez, Adolfo Rodriguez-Eguren, Emilio Frances-Herrero, Marcos Agustina-Hernandez, Amparo Faus, Fernando Gisbert Roca, Cristina Martinez-Ramos, Amparo Galan, Antonio Pellicer, Hortensia Ferrero, Irene Cervello
Summary: The endometrium plays a vital role in fertility and understanding its physiology is crucial for improving reproductive healthcare. This study presents a hybrid endometrial-derived hydrogel that shows promising potential as a biomaterial for regenerative treatments in reproductive medicine. The hydrogel provides excellent support for hEO culture, enhances hEO differentiation efficiency, and demonstrates in vivo compatibility and stability.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Manuel Mata, Santiago Peydro, Jose Javier Martin de Llano, Maria Sancho-Tello, Carmen Carda
Summary: Periodontitis is an inflammatory disease that can lead to tooth loss. This study examined the role of surface microtopography in the differentiation of human dental pulp stem cells into cementoid-like secreting cells. The results showed that the cells cultured on dentin surfaces exhibited an odontoblastic phenotype, while cells cultured on cementum surfaces generated a well-organized tissue consisting of secretory cells and dense fibrous connective tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Beatriz Perez-Benavente, Alihamze Fathinajafabadi, Lorena de la Fuente, Carolina Gandia, Arantxa Martinez-Ferriz, Jose Miguel Pardo-Sanchez, Lara Milian, Ana Conesa, Octavio A. Romero, Julian Carretero, Rune Matthiesen, Isabelle Jariel-Encontre, Marc Piechaczyk, Rosa Farras
Summary: The transcription factor JUNB plays a role in cell division and tumorigenic processes by regulating the expression of cyclin El and transforming growth factor (TGF)-beta 2. High levels of JUNB can switch the response of TGF-beta 2 stimulation from anti-proliferative to pro-invasive. JUNB amplification is associated with poor prognosis in breast and ovarian cancer patients.