Article
Agriculture, Multidisciplinary
Haifei Wang, Yeyi Xiao, Chao Xu, Yue Cao, Pengfei Jing, Shenglong Wu, Jianfeng Liu, Wenbin Bao
Summary: Zearalenone (ZEA) is a mycotoxin that threatens both animal feed and human food safety. This study examined the metabolic and transcriptional changes in porcine IPEC-J2 intestinal epithelial cells upon ZEA exposure and identified key metabolites and regulators involved in ZEA toxicological processes. The results showed that lipid metabolism, amino acid metabolism, and carbohydrate metabolism were most affected. The addition of L-arginine significantly facilitated ZEA metabolism, reduced reactive oxygen species levels and cell apoptosis, and activated autophagic flux. This study provides insights into the cellular response to ZEA exposure and potential targets for biomonitoring and controlling ZEA contamination.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Fisheries
Kaihui Xie, Zunqiang Yan, Wei Wang, Ruirui Luo, Xiaoli Gao, Pengfei Wang, Qiaoli Yang, Xiaoyu Huang, Juanli Zhang, Jiaojiao Yang, Shuangbao Gun
Summary: The study demonstrates that ssc-microR-132 targets DACH1 to alleviate CPB2-mediated inflammation and apoptosis in IPEC-J2.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xiaoli Gao, Qiaoli Yang, Shengwei Zhang, Xiaoyu Huang, Zunqiang Yan, Pengfei Wang, Ruirui Luo, Wei Wang, Kaihui Xie, Shuangbao Gun
Summary: The study found that the significant upregulation of ssc-miR-124a in IPEC-J2 cells after stimulation by C. perfringens beta2-toxin was associated with the demethylation status of the MiR-124A-1 and MiR-124A-2 gene promoters. This upregulation of ssc-miR-124a may help alleviate apoptosis and inflammation in the cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Veterinary Sciences
Zipeng Jiang, Weifa Su, Chaoyue Wen, Wentao Li, Yu Zhang, Tao Gong, Shuai Du, Xinxia Wang, Zeqing Lu, Mingliang Jin, Yizhen Wang
Summary: Clostridium perfringens is a main pathogen that can cause various diseases in humans and animals. This study aimed to build a mouse model infected with porcine C. perfringens and investigated its effects on growth performance, immune status, and intestinal integrity in mice. The results showed that high dose of C. perfringens caused a decline in growth performance, severe damage to intestinal structure, and increased inflammation in mice.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Haixiu Wang, Eric Cox, Bert Devriendt
Summary: Enterotoxigenic Escherichia coli (ETEC) strains recognize and respond to factors secreted by gut epithelial cells, leading to transcriptional modulation of key ETEC virulence genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Environmental Sciences
Song Yu, Lianpeng Zou, Jiawei Zhao, Yiping Zhu
Summary: Fumonisins are common contaminants that pose various health risks. This study demonstrates that resveratrol can protect against fumonisin-induced intestinal toxicity by reducing cell damage, apoptosis, and inflammation. It also provides a new strategy for blocking and controlling the toxicity of fumonisins.
ENVIRONMENTAL TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yuhan Wang, Bing Yu, Yuheng Luo, Ping Zheng, Xiangbing Mao, Zhiqing Huang, Jie Yu, Junqiu Luo, Hui Yan, Aimin Wu, Jun He
Summary: In this study, the protective effects of porcine interferon-lambda 3 (PIFN-lambda 3) on intestinal inflammation and injury induced by porcine rotavirus (PRV) were explored. PIFN-lambda 3 decreased the concentrations of inflammatory cytokines, increased the concentrations of immunoglobulins, and restored the damaged intestinal structure. These findings suggest that PIFN-lambda 3 could be a promising candidate for preventing pathogen-induced bowel diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Pablo Castro-Cordova, Maria Jose Mendoza-Leon, Daniel Paredes-Sabja
Summary: In elderly mice, the internalization of Clostridioides difficile spores in the ileum and colonic mucosa is higher compared to adults or young mice, while spore adherence to the ileum and colonic mucosa decreases with aging.
Article
Multidisciplinary Sciences
Natalia Jaeger, Ramya Gamini, Marina Cella, Jorge L. Schettini, Mattia Bugatti, Shanrong Zhao, Charles V. Rosadini, Ekaterina Esaulova, Blanda Di Luccia, Baylee Kinnett, William Vermi, Maxim N. Artyomov, Thomas A. Wynn, Ramnik J. Xavier, Scott A. Jelinsky, Marco Colonna
Summary: Crohn's disease is a chronic transmural inflammation in the gut caused by dysregulated interaction between microbiome and gut immune system. Studies have shown that T cells from intestinal epithelium and lamina propria in CD patients exhibit unique subsets and altered phenotypes during inflammation.
NATURE COMMUNICATIONS
(2021)
Article
Food Science & Technology
Shuyi Xu, Shiyi Lu, Haichao Wang, Sisi Li, Jie Feng
Summary: The study demonstrates that betaine can protect porcine small intestinal epithelium from hyperosmotic stress by alleviating cell shrinkage, ROS accumulation, as well as apoptosis and autophagy.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Microbiology
Ayesha Lone, Walid Mottawea, Yasmina Ait Chait, Riadh Hammami
Summary: The poultry industry is rapidly growing, but safety concerns and the need for antibiotic alternatives are important. Salmonella and Clostridium perfringens are common pathogens, prompting the search for potential probiotics. Isolated strains showed inhibitory activity against major pathogens, with some exhibiting probiotic characteristics.
Article
Biochemistry & Molecular Biology
Mahmood Karimi Abdolmaleki, Deepak Ganta, Ali Shafiee, Carlo Alberto Velazquez, Devang P. Khambhati
Summary: This study investigates the usage of MOFs for heparin uptake, specifically focusing on the metal-organic framework ZIF-8. Results show that ZIF-8 can recover up to 70% of heparin from porcine intestinal mucosa with appropriate surface conditions for adsorption.
Article
Pharmacology & Pharmacy
Jaiza Samara Macena de Araujo, Maria Cristina Volpato, Bruno Vilela Muniz, Gabriela Gama Augusto Xavier, Claudia Cristina Maia Martinelli, Renata Fonseca Vianna Lopez, Francisco Carlos Groppo, Michelle Franz-Montan
Summary: Permeation assays on swine buccal and esophageal epithelia using electrical resistance (ER) as a method showed that ER is effective for evaluating tissue integrity in vitro. Frozen storage decreased electrical resistivity and increased permeability in both epithelia, with esophageal epithelium exhibiting higher permeability compared to buccal epithelium.
Article
Food Science & Technology
Song Yu, Lianpeng Zou, Jiawei Zhao, Yiping Zhu
Summary: Human health is seriously threatened by mycotoxin contamination, and previous health risk assessments have failed to consider the additive or competitive interactions between co-occurring mycotoxins. This investigation evaluated the toxicological effects of three fumonisin-family B mycotoxins on porcine intestinal epithelial cells. The findings showed that the mycotoxins inhibited cell viability, with FB1 exhibiting greater cytotoxicity than FB2 and FB3. Combinations of these mycotoxins exhibited synergistic or antagonistic effects.
Article
Biotechnology & Applied Microbiology
Jie Li, Kaihui Xie, Jiaojiao Yang, Juanli Zhang, Qiaoli Yang, Pengfei Wang, Shuangbao Gun, Xiaoyu Huang
Summary: This study identified downstream target genes regulated by S100A9 in Clostridium perfringens beta2 (CPB2) toxin-induced IPEC-J2 cell injury using RNA-Seq technique. The results showed that S100A9 was involved in CPB2-induced inflammatory response by regulating the expression of downstream target genes, promoting apoptosis, and aggravating oxidative cell damage. This study laid the foundation for further understanding the regulatory mechanism underlying piglet diarrhea.
Editorial Material
Veterinary Sciences
E. Goossens, E. Dierick, R. Ducatelle, F. Van Immerseel
Article
Gastroenterology & Hepatology
Julien Pujo, Camille Petitfils, Pauline Le Faouder, Venessa Eeckhaut, Gaelle Payros, Sarah Maurel, Teresa Perez--Berezo, Matthias Van Hul, Frederick Barreau, Catherine Blanpied, Stephane Chavanas, Filip Van Immerseel, Justine Bertrand--Michel, Eric Oswald, Claude Knauf, Gilles Dietrich, Patrice D. Cani, Nicolas Cenac
Summary: In this study, it was found that a probiotic strain contained increased levels of 3-hydroxyoctadecaenoic acid (C18-3OH), which was shown to decrease colitis in mice and was associated with anti-inflammatory properties of prebiotic fructooligosaccharides (FOS). The concentration of C18-3OH was correlated with an increase in specific bacterial genera in the microbiota. This suggests that C18-3OH production by bacteria could play a role in the anti-inflammatory properties of probiotics and in microbiota/host interactions.
Article
Agriculture, Dairy & Animal Science
Annatachja De Grande, Richard Ducatelle, Evelyne Delezie, Christof Rapp, Stefaan De Smet, Joris Michiels, Freddy Haesebrouck, Filip Van Immerseel, Saskia Leleu
Summary: The study showed that supplementing broilers with a diet containing zinc amino acid complex and 50 IU/kg of vitamin E had a positive impact on growth performance, including improved weight gain and feed conversion ratio. Additionally, it also enhanced intestinal health indicators.
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
(2021)
Article
Agriculture, Dairy & Animal Science
Evelien Dierick, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
Summary: This research describes the use of experimental models with different timings and frequencies of Eimeria administration to evaluate the incidence and severity of necrotic enteritis. The early repeated coccidial administration resulted in a significant increase in average necrotic lesion score and a higher number of necrotic enteritis-positive animals. These findings contribute to the better standardization of the subclinical necrotic enteritis model and reduction of the required numbers of experimental animals.
Article
Microbiology
Thomas Demoulins, Melanie Brugger, Beatrice Zumkehr, Blandina I. Oliveira Esteves, Kemal Mehinagic, Amal Fahmi, Loic Borcard, Adriano Taddeo, Damian Jandrasits, Horst Posthaus, Charaf Benarafa, Nicolas Ruggli, Marco P. Alves
Summary: Using a model of newborn lambs, researchers found significant differences in the T cell compartment of the neonatal lung compared to the mature adult lung, with RSV infection exacerbating a pro-type 2 environment in the bronchoalveolar space. Regulatory T cells play a key role in dampening the pro-type 2 environment early on, affecting the severity of RSV disease.
Article
Immunology
Lore Van Damme, Natasja Cox, Chana Callens, Michelle Dargatz, Monika Fluegel, Sarah Hark, Frank Thiemann, Stefan Pelzer, Freddy Haesebrouck, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
Summary: Clostridium perfringens produces ECM-degrading enzymes that play a role in avian necrotic enteritis. Different variants of the colA gene encoding collagenase were identified, with some variants linked to necrotic enteritis strains. Truncated collagenases may contribute to the pathology of necrotic enteritis by degrading collagen type IV in the intestinal villi.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Pearl Choi, Lamya Rhayat, Eric Pinloche, Estelle Devillard, Ellen De Paepe, Lynn Vanhaecke, Freddy Haesebrouck, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
Summary: In this study, it was found that administering a Bacillus strain to broiler chickens can change the microbial composition in the gut by producing metabolites that affect beneficial bacterial families and suppress pathogenic bacterial families. The metabolites produced by the strain were shown to have positive effects on both the host and its microbiome, indicating that the probiotic effects of the Bacillus strain are mediated through a combination of metabolites.
Article
Veterinary Sciences
M. Ringenier, N. Caekebeke, F. De Meyer, T. Van Limbergen, V Eeckhaut, R. Ducatelle, F. Van Immerseel, J. Dewulf
Summary: This study evaluated the correlation between macroscopic gut appearance scoring (GAS), coccidiosis lesion scoring, histological measurements, and performance parameters in broilers under field conditions. The findings showed that a higher GAS score was associated with shorter villi and lower CD3+ T-lymphocyte area percentage in the duodenal mucosa, while it did not affect feed conversion ratio (FCR). This suggests that broilers are able to cope with a certain level of gut damage before it influences overall performance.
Article
Microbiology
Martina Husta, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
Summary: The study presents a high-throughput method for assessing the NetB activity of C. perfringens, the causative agent of necrotic enteritis in poultry. It demonstrates a positive correlation between in vitro NetB activity and the virulence of C. perfringens strains, enabling effective screening of strains with disease-inducing potential.
Review
Infectious Diseases
N. J. C. Woittiez, J. van Prehn, F. van Immerseel, E. Goossens, M. P. Bauer, C. L. Ramspek, R. M. E. Slangen, I. M. Purmer, J. Ludikhuize
Summary: Clostridium perfringens infection with intravascular haemolysis is rare but often fatal. Toxinotyping is not regularly performed, and increased use is advocated for better understanding of pathophysiology and more effective interventions.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Food Science & Technology
Basma Tarek, Julia Bruggisser, Filippo Cattalani, Horst Posthaus
Summary: Beta toxin (CPB) is a toxin produced by Clostridium perfringens type C that targets intestinal endothelial cells by interacting with the membrane protein CD31. This study confirms CD31 as the receptor of CPB on human endothelial and monocytic cells, explaining the cell type specificity of CPB observed in vitro and in vivo.
Article
Microbiology
Lore Van Damme, Chana Callens, Michelle Dargatz, Monika Flugel, Sarah Hark, Frank Thiemann, Stefan Pelzer, Richard Ducatelle, Filip Van Immerseel, Evy Goossens
Summary: This study shows the important role of sialidases in the pathogenesis of necrotic enteritis caused by NetB producing Clostridium perfringens. Sialidases degrade the intestinal mucus layer and increase toxin production. Additionally, the use of recombinant sialidase increases bacterial adherence to specific cells.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Emilia Radulovic, Kemal Mehinagic, Tsering Wuethrich, Markus Hilty, Horst Posthaus, Artur Summerfield, Nicolas Ruggli, Charaf Benarafa
Summary: In this study, the immunological profile of specific pathogen-free (SPF) and farm-raised Large White domestic pigs before and after African Swine Fever virus (ASFV) infection was compared. The results showed that SPF pigs had lower immune cell counts and basal inflammatory responses at steady state. When infected with a highly virulent virus strain, SPF pigs showed earlier and more severe clinical signs and pro-inflammatory cytokines, indicating a reduced ability to control early virus replication. In contrast, SPF pigs presented a milder and shorter disease course with full recovery when infected with an attenuated virus strain.
Article
Oncology
Carmen A. Widmer, Ismar Klebic, Natalya Domanitskaya, Morgane Decollogny, Denise Howald, Myriam Siffert, Paul Essers, Zuzanna Nowicka, Nadine Stokar-Regenscheit, Marieke van de Ven, Renske de Korte-Grimmerink, Jose A. Galvan, Colin E. J. Pritchard, Ivo J. Huijbers, Wojciech Fendler, Conchita Vens, Sven Rottenberg
Summary: Our study demonstrates that the lack of expression of Lrrc8a or Lrrc8d significantly reduces the uptake and efficacy of cisplatin and carboplatin in Pt-sensitive BRCA1;p53-deficient tumors. Moreover, our work provides support to confirm the LRRC8A and LRRC8D gene expression in individual tumors prior to initiation of intensive Pt-based chemotherapy.
CANCER RESEARCH COMMUNICATIONS
(2022)
Article
Nutrition & Dietetics
Ya Wang, Evy Goossens, Venessa Eeckhaut, Estefania Perez Calvo, Rual Lopez-Ulibarri, Irene Eising, Mikkel Klausen, Nathan Debunne, Bart De Spiegeleer, Richard Ducatelle, Filip Van Immerseel
Summary: The fungal muramidase derived from Acremonium alcalophilum can hydrolyse PGN to MDP, activating NOD2 and potentially improving intestinal health.
BRITISH JOURNAL OF NUTRITION
(2021)