Article
Urology & Nephrology
Simon Ville, Marine Lorent, Clarisse Kerleau, Anders Asberg, Christophe Legendre, Emmanuel Morelon, Fanny Buron, Valerie Garrigue, Moglie Le Quintrec, Sophie Girerd, Marc Ladriere, Laetitia Albano, Antoine Sicard, Denis Glotz, Carmen Lefaucheur, Julien Branchereau, David Jacobi, Magali Giral
Summary: The study found a lower incidence of delayed graft function in patients whose kidney clamping occurred between midnight and noon, while the declamping time was not associated with kidney graft outcomes.
CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hanno Maassen, Leonie H. Venema, Marc G. Weiss, Tobias M. Huijink, H. Sijbrand Hofker, Anna K. Keller, Tom E. Mollnes, Marco Eijken, Soren E. Pischke, Bente Jespersen, Harry van Goor, Henri G. D. Leuvenink
Summary: Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aimed to improve the flush-out and decrease ischemic injury by adding hydrogen sulphide (H2S) to the flush medium. The addition of H2S during flush out and static cold storage resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers.
Article
Biochemistry & Molecular Biology
Adam Hosszu, Akos R. R. Toth, Tamas Lakat, Ganna Stepanova, Zsuzsanna Antal, Laszlo J. J. Wagner, Attila J. J. Szabo, Andrea Fekete
Summary: Kidney transplantation is the preferred treatment for end-stage kidney disease, and optimizing organ condition is crucial due to the shortage of transplantable organs. This study demonstrated that supplementing the preservation solution with the S1R agonist fluvoxamine reduced cold and warm ischemic injury, improved kidney function, mitigated histological injury, and reduced inflammation. The results suggest that S1R agonists have great potential in improving organ preservation and alleviating the problem of organ shortages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Nora Jahn, Ulrich Sack, Sebastian Stehr, Maria Theresa Voeelker, Sven Laudi, Daniel Seehofer, Selim Atay, Panagiota Zgoura, Richard Viebahn, Andreas Boldt, Hans-Michael Hau
Summary: Peripheral blood innate immune cells and clinical factors can be used to predict early renal graft injury (eRGI) after kidney transplantation. Monocyte and NK cell levels on postoperative day 7 are highly predictive for eRGI. Patient age, body mass index, cardiovascular disease, donor age, donor source, and cold ischemia time are also associated with eRGI.
JOURNAL OF CLINICAL MEDICINE
(2022)
Review
Medicine, General & Internal
Ruta Zulpaite, Povilas Miknevicius, Bettina Leber, Kestutis Strupas, Philipp Stiegler, Peter Schemmer
Summary: Kidney transplantation is the gold standard treatment for patients with end-stage kidney disease, but the quality of donor organs poses limitations. Ex-vivo machine perfusion technology has great potential in maintaining kidney viability and optimizing organ treatment, serving as an important component of novel techniques in transplant surgery.
FRONTIERS IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Eileen Edgworth, Lisa Ernst, Zoltan Czigany, Turgay Saritas, Laura Sophie Zarnitz, Marc Wiartalla, Peter Boor, Eva Miriam Buhl, Rolf Rossaint, Rene H. Tolba, Benedict Doorschodt, Gregor Fabry, Christian Bleilevens
Summary: Normothermic machine perfusion (NMP) combined with optimized perfusate composition can improve the quality of kidney preservation, but the optimization of perfusates is still under research. Hemoglobin-based oxygen carriers (HBOCs), such as HBOC-301, can be an alternative to packed red blood cells (pRBC), but they can cause methemoglobin formation and induce hypoxia. The addition of ascorbic acid (VitC) as an antioxidant may have a beneficial effect. This study showed that HBOC-301 is inferior to pRBCs in a porcine kidney NMP model, regardless of the addition of VitC. Oxidative stress and fragmentation of hemoglobin polymers may contribute to these results, indicating the need for further research on cell-free oxygen carriers without these issues.
Review
Biochemistry & Molecular Biology
Maria Irene Bellini, Francesco Tortorici, Maria Ida Amabile, Vito D'Andrea
Summary: Kidney transplantation is the preferred treatment for end-stage renal disease, with static cold storage being the standard preservation method. However, dynamic techniques like machine perfusion have shown potential to improve graft function and quality assessment, particularly in kidneys from extended criteria donors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, General & Internal
Sara Akalay, Sarah A. Hosgood
Summary: Although machine perfusion is gradually being applied in clinical practice, the improvement of organ reconditioning therapies is still at the experimental stage, showing a translational gap. This review discusses the biological processes and current strategies and interventions for preventing ischemia-reperfusion injury, treating its deleterious consequences, or supporting the reparative response of the kidney, and explores prospects to improve the clinical translation of these therapies by addressing multiple aspects of ischemia-reperfusion injury to achieve robust and long-lasting protective effects on the kidney graft.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Florian Emmerich, Stefan Zschiedrich, Christine Reichenbach-Braun, Caner Susal, Susana Minguet, Marie-Christin Pauly, Maximilian Seidl
Summary: Higher Cav-1 levels are associated with lower occurrence of acute cellular tubulointerstitial rejection episodes.
Review
Medicine, Research & Experimental
Fatemeh Sabet Sarvestani, Negar Azarpira, Ismail H. Al-Abdullah, Ali-Mohammad Tamaddon
Summary: Ischemia reperfusion injury can lead to tissue damage, especially during organ transplantation. By manipulating miRNAs level, they can be used as a biomarker for early diagnosis of IRI or suggestive to be therapeutic agents in clinical situation in future.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Medicine, General & Internal
Stephan Kemmner, Christopher Holzmann-Littig, Helene Sandberger, Quirin Bachmann, Flora Haberfellner, Carlos Torrez, Christoph Schmaderer, Uwe Heemann, Lutz Renders, Volker Assfalg, Tarek M. El-Achkar, Pranav S. Garimella, Juergen Scherberich, Dominik Steubl
Summary: Higher pretransplant serum uromodulin (sUMOD) levels were independently associated with lower odds of delayed graft function (DGF) following kidney transplantation, potentially serving as a non-invasive marker for stratifying patients based on their risk for developing DGF early on.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Immunology
Biao Lei, M. Mahdi Sleiman, Qi Cheng, Zhenxiao Tu, Peng Zhu, Martin Goddard, Paulo N. Martins, Logan Langerude, Satish Nadig, Stephen Tomlinson, Carl Atkinson
Summary: This study found that donor brain death exacerbates ischemia-reperfusion injury in vascularized composite allograft transplantation and leads to earlier rejection of the graft. Treatment of donor VCAs with CR2-Crry significantly reduced complement deposition, inflammation, and injury, providing localized protection without significant immunosuppression effects.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Qi Peng, Anna Nowocin, Kulachelvy Ratnasothy, Richard A. Smith, Lesley A. Smyth, Robert I. Lechler, Anthony Dorling, Giovanna Lombardi
Summary: Thrombin inhibition enhances the infiltration of anti-inflammatory cells and improves renal ischemia-reperfusion injury. In a transplant model, combined therapy of thrombin inhibition and regulatory T cell infusion leads to prolonged graft survival and reduced inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Surgery
Joana Ferrer-Fabrega, Emma Folch-Puy, Juan Jose Lozano, Pedro Ventura-Aguiar, Gabriel Cardenas, David Paredes, Angeles Garcia-Criado, Josep Antoni Bombi, Rocio Garcia-Perez, Miguel Angel Lopez-Boado, Ramon Rull, Enric Esmatjes, Maria Jose Ricart, Fritz Diekmann, Constantino Fondevila, Laureano Fernandez-Cruz, Josep Fuster, Juan Carlos Garcia-Valdecasas
Summary: This study retrospectively analyzed data from 380 pancreas transplants and compared the impact of different preservation solutions on transplant outcomes. The results showed that the IGL-1 solution had better performance in perioperative complications and higher survival rates at 1, 3, and 5 years compared to other solutions. Therefore, using the IGL-1 solution for perfusion and organ preservation may be a promising choice in clinical pancreas transplantation.
TRANSPLANT INTERNATIONAL
(2022)
Article
Medicine, General & Internal
Nora Jahn, Maria Theresa Voelker, Sven Laudi, Sebastian Stehr, Stefan Schneeberger, Gerald Brandacher, Elisabeth Sucher, Sebastian Rademacher, Daniel Seehofer, Hans Michael Hau, Robert Sucher
Summary: This study investigated the effects of different volatile anesthetics on early ischemia-reperfusion injury (IRI)-associated clinical outcomes in pancreas transplantation recipients. The results showed that the use of sevoflurane and desflurane was associated with increased early graft survival and decreased IRI-related postoperative complications.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ophelie Pasini-Chabot, Julia Vincent, Sylvain Le Pape, Maryne Lepoittevin, Wassim Kaaki, Jean-Baptiste Woillard, Sebastien Giraud, Nicolas Bourmeyster, Thierry Hauet, Raphael Thuillier
Summary: The study indicates that significant changes in protein expression occur in key pathways during organ preservation, with time-sensitive protein expression potentially serving as quality biomarkers for organs and targets for new strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Leonie H. Venema, L. Leonie van Leeuwen, Rene A. Posma, Harry van Goor, Rutger J. Ploeg, Patrick Hannaert, Thierry Hauet, Thomas Minor, Henri G. D. Leuvenink
Summary: This study compared the effectiveness of two blood substitute solutions, one with red blood cells (RBCs) and one without, in kidney evaluation. The results showed that the addition of RBCs reduced renal injury, improved function, and increased renal metabolism. However, a blood-based perfusion solution was still superior.
Article
Engineering, Biomedical
Yannick Le Meur, Eric Delpy, Felix Renard, Thierry Hauet, Lionel Badet, Jean Philippe Rerolle, Antoine Thierry, Matthias Buchler, Franck Zal, Benoit Barrou
Summary: The study evaluated the safety and efficacy of using HEMO(2)life (R) as an additive to preservation solution in renal transplantation, showing lower DGF rates and better renal function in the HEMO(2)life (R) group. Analysis using Kaplan-Meier estimate curves, Cox model, and Bootstrap confirmed the advantages of the HEMO(2)life (R) group.
Article
Biochemistry & Molecular Biology
Pierre-Jean Ferron, Brendan Le Dare, Julie Bronsard, Clara Steichen, Elodie Babina, Romain Pelletier, Thierry Hauet, Isabelle Morel, Karin Tarte, Florian Reizine, Bruno Clement, Bernard Fromenty, Thomas Gicquel
Summary: The study investigated the effects of using hydroxychloroquine (HCQ) to treat COVID-19 patients, especially obese patients, and its impact on drug metabolism and hepatotoxicity. In vitro experiments on HepaRG and RPTEC cells showed that HCQ metabolism was altered in conditions of hepatic steatosis in HepaRG cells, leading to the detection of a new metabolite (carboxychloroquine). Furthermore, HepaRG cells exposed to exogenous fatty acids exhibited increased cytotoxicity of HCQ and enhanced neutral lipid accumulation. These in vitro findings were compared with clinical data from COVID-19 patients treated with HCQ, suggesting that steatosis may increase the risk of altered drug metabolism and potential toxicity of HCQ.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, General & Internal
S. Giraud, R. Thuillier, T. Kerforne, B. Barrou, L. Badet, P. Bucur, E. Salame, C. Goumard, E. Savier, J. Branchereau, P. Battistella, O. Mercier, S. Mussot, T. Hauet
Summary: Organ transplantation is the last resort for patients with terminal organ failure. In France, only about 25% of patients on the waiting list can receive a transplant each year. The consideration of new donor populations has led to the need for better management of organ donation and optimization of storage conditions.
BULLETIN DE L ACADEMIE NATIONALE DE MEDECINE
(2022)
Review
Biochemistry & Molecular Biology
Maryne Lepoittevin, Sebastien Giraud, Thomas Kerforne, Benoit Barrou, Lionel Badet, Petru Bucur, Ephrem Salame, Claire Goumard, Eric Savier, Julien Branchereau, Pascal Battistella, Olaf Mercier, Sacha Mussot, Thierry Hauet, Raphael Thuillier
Summary: Organ transplantation is the last resort for treating organ failure, but the shortage of organs remains a challenge. To address this issue, there is a need to expand the donor pool and optimize preservation conditions to improve the quality of transplanted organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Maryne Lepoittevin, Thomas Kerforne, Luc Pellerin, Thierry Hauet, Raphael Thuillier
Summary: The emerging field of molecular predictive medicine is aiming to change the traditional medical approach in renal transplantation by exploring potential biomarker molecules with predictive properties to improve transplantation success. Current research indicates that molecules associated with kidney transplant outcomes mainly relate to inflammation and immune response, as well as other molecular ontologies such as proteasome, growth, and drug metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Valentin Bossard, Nicolas Bourmeyster, Sophie Pasini, Pierre Dupuis, Souleiman El Balkhi, Emmanuel Richard, Hugo Alarcan, Thierry Hauet, Raphael Thuillier
Summary: Due to the increased prevalence of obesity in the world, bariatric surgeries are on the rise and necessitate life-long surveillance for deficiencies. However, inadequate multivitamin supplementation may induce vitamin B6 overload.
Editorial Material
Biochemistry & Molecular Biology
Thierry Hauet, Didier F. Pisani
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Anatomy & Morphology
Beya Robert, Danion Jerome, Vendeuvre Tanguy, Nguyen My-Van, Renault Arthur, Richer Jean-Pierre, Hauet Thierry, Breque Cyril, Faure Jean-Pierre
Summary: This study compared two models of cadaveric dissection to evaluate the importance of the perfused SimLife((R)) technology in identifying the vascular loop in the pelvic region and its dynamic anatomy. The results showed that the perfused model B made it easier to identify and dissect the vascular loop.
SURGICAL AND RADIOLOGIC ANATOMY
(2023)
Article
Cell Biology
Nicolas Melis, Isabelle Rubera, Sebastien Giraud, Marc Cougnon, Christophe Duranton, Mallorie Poet, Gisele Jarretou, Raphael Thuillier, Laurent Counillon, Thierry Hauet, Luc Pellerin, Michel Tauc, Didier F. Pisani
Summary: Through kidney transplantation, ischemia/reperfusion induces tissular injury due to energy shortage, oxidative stress, and endoplasmic reticulum (ER) stress. We found that the specific inhibitor of eukaryotic Initiation Factor 5A (eIF5A) hypusination, GC7, protects kidney cells by modulating unfolded protein response (UPR) pathways. GC7 pretreatment enhances xbp1 splicing and BiP expression while reducing eIF2 alpha phosphorylation and ATF6 nuclear level, indicating a specific modulation of UPR pathways. Inhibition of xbp1 splicing reverses the protective effect of GC7 against anoxia, demonstrating the crucial role of eIF5A hypusination inhibition in UPR modulation.
Editorial Material
Biochemistry & Molecular Biology
Raphael Thuillier
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Meeting Abstract
Immunology
Perrine Burdeyron, Sebastien Giraud, Sonia Brishoual, Estelle Lemarie, Maite Jacquard, Virginie Ameteau, Nadege Boildieu, Hanno Maassen, Tobias M. Huijink, Luc Pellerin, Henri Leuvenink, Thierry Hauet, Clara steichen