Article
Immunology
Jovana Sadlova, Lucie Podesvova, Tomas Becvar, Claretta Bianchi, Evgeny S. Gerasimov, Andreu Saura, Kristyna Glanzova, Tereza Lestinova, Nadezhda S. Matveeva, Lubomira Chmelova, Denisa Mlacovska, Tatiana Spitzova, Barbora Vojtkova, Petr Volf, Vyacheslav Yurchenko, Natalya Kraeva
Summary: Research shows that expressing catalase in Leishmania parasites severely compromises their ability to develop in insects, be transmitted, infect mice, and cause clinical manifestation in mammalian hosts. This suggests that the presence of catalase is not compatible with the dixenous life cycle of Leishmania.
Article
Immunology
Lenka Pacakova, Karel Harant, Petr Volf, Tereza Lestinova
Summary: This study compared the proteome of Leishmania mexicana amastigotes from lesion of infected BALB/c mice with alternately cultivated amastigotes. Significant differences in the abundance of metabolic enzymes, virulence factors and proteins involved in translation and condensation of DNA were observed, with axenic amastigotes showing the most pronounced differences compared to lesion-derived amastigotes, and macrophage-derived amastigotes mostly intermediate between the two.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Xiu-ting Liu, Zhen-rui Shi, Si-yao Lu, Dan Hong, Xiao-nan Qiu, Guo-zhen Tan, Hui Xiong, Qing Guo, Liangchun Wang
Summary: Microabscess of neutrophils in epidermis is a histological hallmark of psoriasis. This study found that blood neutrophils were increased in psoriasis patients, positively correlated with disease severity, and highly expressed CD66b. Keratinocytes expressed high levels of psoriasis-related inflammatory mediators through interaction with neutrophils, and this interaction was dependent on IL-17A produced by neutrophils. Neutrophils from psoriasis patients displayed more transcriptome changes related to integrin and increased migration capacity toward keratinocytes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Parasitology
Mayra A. Rodriguez-Serrato, Angel F. Gonzalez-Mireles, Alberto Y. Limon-Flores, Mario C. Salinas-Carmona
Summary: Cutaneous leishmaniasis is the most common form of leishmaniasis in humans, and UVB radiation may play an important role in this disease by suppressing immune responses and promoting lesion progression.
Review
Immunology
Matheus B. Carneiro, Nathan C. Peters
Summary: This review focuses on the interaction between Leishmania amazonensis infection and host innate immune response, particularly addressing the impact of adaptive immunity on phagocytic host cell recruitment and activation. Experimental observations indicate that most mouse strains are susceptible to L. amazonensis infection, unlike other Leishmania species. The CD4(+) T helper (Th)1/Th2 paradigm does not fully explain the chronic disease established by L. amazonensis, with recent findings complicating the understanding of the Th1/Th2 paradigm.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Karla Fabiola Chacon-Vargas, Luvia Enid Sanchez-Torres, Monica L. Chavez-Gonzalez, Jaime R. Adame-Gallegos, Guadalupe Virginia Nevarez-Moorillon
Summary: This study examined the leishmanicidal effect and mechanism of cell death induced by Mexican oregano essential oils and their main components on Leishmania mexicana promastigotes. The results showed that Mexican oregano essential oil and its main component, thymol, exhibited high leishmanicidal activity and low cytotoxic activity in mammalian cells. Apoptosis was identified as the main mechanism of death induced in the parasites.
Article
Microbiology
Hasnaa Maksouri, Dounia Darif, Jerome Estaquier, Myriam Riyad, Christophe Desterke, Meryem Lemrani, Pham My-Chan Dang, Khadija Akarid
Summary: This study evaluated the impact of Moroccan strains of L. major and L. tropica on PMNs. The results showed that L. tropica inhibited O-2(-) production, while L. major did not. Additionally, Leishmania soluble antigens from both species inhibited O-2(-) induction.
Article
Medicine, General & Internal
Md Anik Ashfaq Khan, Khaledul Faisal, Rajashree Chowdhury, Prakash Ghosh, Faria Hossain, Manfred Weidmann, Dinesh Mondal, Ahmed Abd El Wahed
Summary: This study developed a quantification method to assess the absolute quantification ability of isothermal recombinase polymerase amplification (RPA) assay for parasite genomes. The findings suggest the potential of Q-RPA assay in monitoring parasite load and developing rapid assays for other infectious diseases.
Article
Microbiology
Laura Sanchez-Garcia, Armando Perez-Torres, Samira Munoz-Cruz, Norma Salaiza-Suazo, Jorge Morales-Montor, Ingeborg Becker
Summary: The presence of male sex hormones modulates the mast-cell response to Leishmania infections and salivary proteins of the sand fly vector, inducing an intense inflammatory response. Their absence in orchiectomized mice results in a delayed inflammatory response, enabling better control of the infection and slower disease progression.
Article
Microbiology
Sarah D'Alessandro, Silvia Parapini, Yolanda Corbett, Roberta Frigerio, Serena Delbue, Annalisa Modenese, Marina Gramiccia, Pasquale Ferrante, Donatella Taramelli, Nicoletta Basilico
Summary: The interaction between Leishmania promastigotes and endothelial cells leads to the production of chemokines and the recruitment of neutrophils, contributing to the establishment of Leishmania infection.
Review
Immunology
Katiuska Passelli, Oaklyne Billion, Fabienne Tacchini-Cottier
Summary: Leishmania parasites, transmitted by infected sand flies, cause a range of diseases in mammals. Neutrophils are crucial in the defense against these parasites, although some Leishmania species have developed ways to survive within them. Neutrophils also play a role in shaping the skin microenvironment during infection, influencing the adaptive immune response and disease outcome.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Kai Liu, Hui-Huang Huang, Tao Yang, Yan-Mei Jiao, Chao Zhang, Jin-Wen Song, Ji-Yuan Zhang, Chun-Bao Zhou, Jin-Hong Yuan, Wen-Jing Cao, Xiu-Ying Mu, Ming-Ju Zhou, Hua-Jie Li, Ming Shi, Ruonan Xu, Fu-Sheng Wang
Summary: Neutrophils in HIV-1 infection undergo aging and over-aggregation, leading to T cell dysfunction. Dampening neutrophil aging may provide a novel approach to recover T cell dysfunction in patients with HIV-1 infection. So it's important to study the dynamics of neutrophil aging and its relationship with T cell exhaustion.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Construction & Building Technology
Songhui Liu, Li Zhang, Dongxing Xuan, Peiliang Shen, Jianping Zhu, Xuemao Guan, Caijun Shi
Summary: The study demonstrates that rapid cooling process can enhance the carbonation reactivity of wollastonite, leading to better CO2 sequestration capacity and compressive strength. This research provides new insights for the application of highly reactive CS.
CONSTRUCTION AND BUILDING MATERIALS
(2021)
Article
Immunology
Fernanda Tomiotto-Pellissier, Milena Menegazzo Miranda-Sapla, Taylon Felipe Silva, Bruna Taciane da Silva Bortoleti, Manoela Daiele Goncalves, Virginia Marcia Concato, Ana Carolina Jacob Rodrigues, Mariana Barbosa Detoni, Idessania Nazareth Costa, Carolina Panis, Ivete Conchon-Costa, Juliano Bordignon, Wander Rogerio Pavanelli
Summary: This study revealed that BALB/c mice had worse disease outcomes than C57BL/6 mice when infected with Leishmania amazonensis, with higher parasitic load and elevated levels of IL-6. C57BL/6 mice showed a more effective immune response, characterized by higher levels of IFN-gamma and superoxide anion. Adoptive transfer of macrophages from C57BL/6 to BALB/c mice resulted in improved outcomes, indicating the importance of Arg-1 related macrophages in the pathogenesis of cutaneous leishmaniasis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Parasitology
Christian Florian Teh-Poot, Victor Manuel Dzul-Huchim, Jonathan M. Mercado, Liliana Estefania Villanueva-Lizama, Maria Elena Bottazzi, Kathryn M. Jones, Francis T. F. Tsai, Julio Vladimir Cruz-Chan
Summary: This study established a convenient method to assess the differentiation status of Leishmania mexicana using flow cytometry, and found that flow cytometry can reliably quantify parasite differentiation in cell culture as light microscopy. Additionally, miltefosine was shown to reduce the promastigote-to-amastigote differentiation of L. mexicana. Flow cytometry provides a rapid means to assay the efficacy of small molecules or natural compounds as potential anti-leishmanial drugs.
EXPERIMENTAL PARASITOLOGY
(2023)
Review
Immunology
Shuang Peng, Jian Gao, Darko Stojkov, Shida Yousefi, Hans-Uwe Simon
Summary: Neutrophils, the most abundant innate immune cells in human blood, have been overlooked in terms of their mitochondria function. However, growing evidence shows that mitochondria play a crucial role in various aspects of neutrophil biology, from development to chemotaxis, effector function, and cell death. Mitochondria and their components can also be released by neutrophils to eliminate infection and shape immune response. This review highlights the importance of mitochondria in modulating neutrophil phenotype and function during infection and inflammation, and discusses the potential and challenges of exploiting mitochondria in neutrophils for disease treatment.
IMMUNOLOGICAL REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Aref Hosseini, Darko Stojkov, Timothee Fettrelet, Rostyslav Bilyy, Shida Yousefi, Hans-Uwe Simon
Summary: Neutrophil extracellular traps (NETs) and oxidative stress play important roles in the immune defense against pathogens. However, impaired clearance of NETs in the lungs of COVID-19 patients may contribute to severe respiratory syndrome infection. Through RNA sequencing (RNA-seq) and immunofluorescence and immunohistochemistry methods, the study identified increased oxidative stress and viral-related signaling pathways in COVID-19 patients' lungs. Excess neutrophil infiltration and NET formation, with oxidized extracellular DNA co-localizing with myeloperoxidase (MPO), were observed. The lipid peroxidation marker 4-hydroxynonenal (4-HNE) showed high colocalization with NETs and correlated with lung neutrophil infiltration, suggesting its potential as a marker for identifying NETs and disease severity. Local inhalation therapy to reduce lipid oxidation and NETs may benefit severely infected patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Darko Stojkov, Meike J. Claus, Evelyne Kozlowski, Kevin Oberson, Olivier P. Scharen, Charaf Benarafa, Shida Yousefi, Hans-Uwe Simon
Summary: Neutrophil extracellular traps (NETs) are released by neutrophils as DNA scaffolds coated with granule proteins to ensnare and kill bacteria. The formation of NETs can occur through independent molecular pathways in response to various stimuli. It has been thought that NET release is associated with lytic cell death, but live neutrophils can release antimicrobial NETs rapidly. Gasdermin D (GSDMD), which causes pyroptotic death in macrophages, is believed to be essential for NET formation in neutrophils. However, the study found that GSDMD is not required for NET formation and that viable neutrophils can form NETs after inflammasome activation through both canonical and noncanonical signaling pathways.
Article
Immunology
Bakhos Jneid, Aurore Bochnakian, Caroline Hoffmann, Fabien Delisle, Emeline Djacoto, Philemon Sirven, Jordan Denizeau, Christine Sedlik, Yohan Gerber-Ferder, Frederic Fiore, Ramazan Akyol, Carine Brousse, Robert Kramer, Ian Walters, Sylvain Carlioz, Helene Salmon, Bernard Malissen, Marc Dalod, Eliane Piaggio, Nicolas Manel
Summary: Activation of STING in immunogenic tumors can induce antitumor T cell response, but it remains unclear how to apply it in nonimmunogenic tumors. This study showed that intratumoral delivery of cGAMP-VLP activated T cells and reduced tumor regulatory T cells, synergizing with PD1 blockade. However, intratumoral administration of the synthetic CDN ADU-S100 caused tumor necrosis and systemic T cell activation, but depleted immune cells and minimally primed circulating tumor-specific T cells. Thus, cell targeting of STING stimulation shapes the antitumor T cell response and provides a strategy to enhance T cell-targeted immunotherapy.
SCIENCE IMMUNOLOGY
(2023)
Review
Allergy
Lea Gigon, Timothee Fettrelet, Shida Yousefi, Dagmar Simon, Hans-Uwe Simon
Summary: Eosinophils are bone marrow-derived granulocytes that increase in number during type 2 inflammatory diseases. They migrate to various tissues and organs and have multiple functions through the synthesis and release of granule proteins and pro-inflammatory mediators. The functional role of eosinophils is still debated but they may play a role in host defense and tissue homeostasis.
Article
Biology
Warren Anderson, Fariba Barahmand-pour-Whitman, Peter S. Linsley, Karen Cerosaletti, Jane H. Buckner, David J. Rawlings, Bernard Malissen
Summary: A genetic variant in the PTPN22 gene increases the risk for multiple autoimmune diseases and affects T cell regulation and activation. Using gene editing, researchers created T cells with different PTPN22 variants and found that the risk variant and PTPN22 knockout both led to increased T cell activation. They also observed enhanced signaling and proliferation in T cells with self-reactive T cell receptors lacking PTPN22 function. These findings suggest that PTPN22 rs2476601 contributes to autoimmunity risk by promoting TCR signaling and activation in mildly self-reactive T cells.
Article
Oncology
Clemence Demerle, Laurent Gorvel, Marielle Mello, Sonia Pastor, Clara Degos, Ana Zarubica, Fabien Angelis, Frederic Fiore, Jacques A. Nunes, Bernard Malissen, Laurent Greillier, Geoffrey Guittard, Herve Luche, Fabrice Barlesi, Daniel Olive
Summary: The study found that anti-HVEM18-10 can enhance the activity of human αβ-T cells and trigger T cell activation in the presence of PD-L1-negative cells. Anti-HVEM18-10 treatment inhibits the growth of HVEM-positive tumors in mouse models and promotes the presence of effector memory CD4+ T cells while suppressing exhausted CD8+ T cells and regulatory T cells.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Biology
Richard Miallot, Virginie Millet, Yann Groult, Angelika Modelska, Lydie Crescence, Sandrine Roulland, Sandrine Henri, Bernard Malissen, Nicolas Brouilly, Laurence Panicot-Dubois, Renaud Vincentelli, Gerlind Sulzenbacher, Pascal Finetti, Aurelie Dutour, Jean-Yves Blay, Francois Bertucci, Franck Galland, Philippe Naquet
Summary: Aggressive tumors often have dysfunctional mitochondria. OMA1 mediates fission of mitochondria in response to oxidative stress through cleavage of the fusion effector OPA1. In this study, mutation of cysteine 403 in OMA1 impaired mitochondrial responses to stress, resulting in reduced ATP production, resistance to apoptosis, and enhanced mitochondrial DNA release. Inactivation of OMA1 increased anti-tumor immunity and may enhance sarcoma immunogenicity.
LIFE SCIENCE ALLIANCE
(2023)
Article
Immunology
Anais Joachim, Rudy Aussel, Lena Gelard, Fanghui Zhang, Daiki Mori, Claude Gregoire, Sergio Villazala Merino, Mauro Gaya, Yinming Liang, Marie Malissen, Bernard Malissen
Summary: Mice with a loss-of-function mutation in the LAT adaptor (Lat(Y136F)) develop an autoimmune and type 2 inflammatory disorder called defective LAT signalosome pathology (DLSP). Through single-cell omics analysis, it was found that T follicular helper cells, CD4+ cytotoxic T cells, activated B cells, and plasma cells are present in Lat(Y136F) spleen and lung. This cell constellation is similar to the cell types involved in IgG4-related disease (IgG4-RD), indicating that Lat(Y136F) DLSP can serve as a model for IgG4-RD.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Biology
Richard Miallot, Virginie Millet, Anais Roger, Romain Fenouil, Catherine Tardivel, Jean-Charles Martin, Laetitia Shintu, Paul Berchard, Juliane Sousa Lanza, Bernard Malissen, Sandrine Henri, Sophie Ugolini, Aurelie Dutour, Pascal Finetti, Francois Bertucci, Jean-Yves Blay, Franck Galland, Philippe Naquet
Summary: The study found that pantethine can inhibit tumor growth by enhancing the function of the immune system. Pantethine can promote the polarization of myeloid and dendritic cells towards enhanced antigen presentation pathways and improve the development of CD8+ T cells with immune effector activity. However, immune cell exhaustion limits the therapeutic effect of pantethine.
LIFE SCIENCE ALLIANCE
(2023)
Meeting Abstract
Oncology
Herve Luche, Lillia Hadjem, Marielle Mello, Priscilla Canavese, Fabien Angelis, Anais Joachim, Sylvie Bouilly, Frederic Guinut, Frederic Fiore, Bernard Malissen, Ana Zarubica, Erwan Corcuff
Meeting Abstract
Oncology
Laurent Gorvel, Clemence Demerle, Marielle Mello, Sonia Pastor, Clara Degos, Ana Zarubica, Fabien Angelis, Frederic Fiore, Jacques Nunes, Bernard Malissen, Laurent Greillier, Geoffrey Guittard, Herve Luche, Fabrice Barlesi, Daniel Olive
Correction
Oncology
C. Demerle, L. Gorvel, M. Mello
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Review
Oncology
K. F. Idrisova, H. -U. Simon, M. O. Gomzikova
Summary: It has been discovered that traditional tumor-derived cell lines do not accurately replicate the drug response and behavior of real human cancers. Consequently, more reliable tumor models that mimic the heterogeneity and patho-physiology of patient tumors are being developed. This review aims to outline the current patient-derived models of cancer, discuss their pros and cons, and highlight the progress being made in clinical trials and research in this field.