Article
Biochemistry & Molecular Biology
Umakanta Swain, Gilgi Friedlander, Urmila Sehrawat, Avital Sarusi-Portuguez, Ron Rotkopf, Charlotte Ebert, Tamar Paz-Elizur, Rivka Dikstein, Thomas Carell, Nicholas E. Geacintov, Zvi Livneh
Summary: TENT4A plays a critical role in regulating multiple biological pathways, specifically in translesion DNA synthesis, by modulating mRNA stability and translation of DNA polymerase eta and RAD18 E3 ligase. Additionally, TENT4A indirectly regulates RAD18 through the tumor suppressor CYLD and the long non-coding antisense RNA PAXIP1-AS2, leading to dysregulation of TLS in endometrial cancer genomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
So-Young Yoon, Eunhong Jang, Naho Ko, Minseok Kim, Su Yoon Kim, Yeojin Moon, Jeong-Seok Nam, Sunjae Lee, Youngsoo Jun
Summary: The asymmetry in cytosolic pH between mother and daughter cells is believed to be responsible for cellular aging in budding yeast. Preferential accumulation of Pma1p in mother cells, which reduces the level of cytoplasmic protons, is thought to contribute to this pH increase. However, this study found that the accumulation of Pma1p in mother cells is not the key determinant of aging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Helene J. Bustad, Juha P. Kallio, Mikko Laitaoja, Karen Toska, Inari Kursula, Aurora Martinez, Janne Janis
Summary: Research showed structural differences between AIP-associated mutants and wild-type PBGD, and highlighted the significant role of Arg173 in the polypyrrole elongation mechanism.
Article
Biochemistry & Molecular Biology
Olivier Finet, Carlo Yague-Sanz, Lara Katharina Kruger, Phong Tran, Valerie Migeot, Max Louski, Alicia Nevers, Mathieu Rougemaille, Jingjing Sun, Felix G. M. Ernst, Ludivine Wacheul, Maxime Wery, Antonin Morillon, Peter Dedon, Denis L. J. Lafontaine, Damien Hermand
Summary: This study reveals the importance of RNA modifications in gene expression control, and develops a new integrated pipeline to detect modifications in a transcriptome-wide manner. The researchers identify a novel RNA modification and demonstrate its biological function in mRNA of fission yeast. They also find that the modification is evolutionarily conserved in human cells.
Article
Pharmacology & Pharmacy
Xi Liu, Qian Hu, Wanyan Wang, Hui Ma, Jiaqian Pu, Jiayan Cui, Ting Gong, Yu Wu, Weiqiang Lu, Jin Huang
Summary: Gain-of-function mutations in estrogen receptor alpha (ER alpha) are associated with hormone therapy resistance in breast cancer. A new GLPCA method was developed to evaluate ER alpha mutants, identifying natural products celastrol and gambogic acid as antagonists, which showed synergistic anti-cancer effects when combined with CDK4/6 inhibitor abemaciclib.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aldo Spanjaard, Ronak Shah, Daniel de Groot, Olimpia Alessandra Buoninfante, Ben Morris, Cor Lieftink, Colin Pritchard, Lisa M. Zurcher, Shirley Ormel, Joyce J. Catsman, Renske De Korte-Grimmerink, Bjorn Siteur, Natalie Proost, Terry Boadum, Marieke van de Ven, Ji-Ying Song, Maaike Kreft, Paul C. M. van den Berk, Roderick L. Beijersbergen, Heinz Jacobs
Summary: PCNA-ubiquitination and REV1 play distinct roles in DNA damage tolerance, particularly in tolerating cisplatin lesions. Polymerase Kappa is essential in tolerating multiple genotoxic lesions, including cisplatin lesions.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Michelle K. Scotland, Caleb Homiski, Mark D. Sutton
Summary: Specialized DNA polymerases (Pols) capable of translesion synthesis (TLS) generate mutations that contribute to bacterial virulence, pathoadaptation, and antimicrobial resistance. The mechanism by which bacterial TLS Pol IV gains access to the DNA involves exchanging places with bacterial Pol III replicase via a Pol III-Pol IV switch, which may be regulated by multiple interactions between Pol III and Pol IV. Furthermore, several additional E. coli Pol-Pol interactions have been described, suggesting their fundamental roles in coordinating bacterial DNA replication, DNA repair, and TLS.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kun Huang, Xiao-Xian Wu, Cheng-Li Fang, Zhou-Geng Xu, Hong-Wei Zhang, Jian Gao, Chuan-Miao Zhou, Lin-Lin You, Zhan-Xi Gu, Wen-Hui Mu, Yu Feng, Jia-Wei Wang, Yu Zhang
Summary: DNA methylation plays a crucial role in gene expression and genome integrity in plants. The complex formed by Pol IV and RDR2 is involved in dsRNA synthesis, revealing a mechanism of "backtracking-triggered RNA channeling".
Article
Biochemistry & Molecular Biology
Maria Soledad Alvarez, Estefania Nunez, Marina Fuertes-Agudo, Carme Cucarella, Maria Fernandez-Velasco, Lisardo Bosca, Jesus Vazquez, Rodrigue Rossignol, Paloma Martin-Sanz, Marta Casado
Summary: This study suggests a new association between COX-2 and mitochondria, which may contribute to increased expression of respiratory chain complex IV proteins, enhanced respiratory capacity, and increased ATP levels in the hearts of COX-2 transgenic mice, thus providing protective effects against ischemia-reperfusion injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Jun Li, Yanna Wei, Jia Wang, Yao Li, Guoqing Shao, Zhixin Feng, Qiyan Xiong
Summary: This study investigates the development of fluoroquinolone resistance in Mycoplasma hyorhinis and identifies the mechanisms of resistance. Point mutations in the quinolone resistance-determining regions (QRDRs) were observed, with initial mutations occurring in ParC, followed by additional mutations in GyrA and/or ParE. The study also observed target mutations in field strains. Understanding these mutations can aid in rapid detection of fluoroquinolone resistance in field isolates.
Article
Biology
Naima G. Sharaf, Mona Shahgholi, Esther Kim, Jeffrey Y. Lai, David G. VanderVelde, Allen T. Lee, Douglas C. Rees
Summary: NmMetQ is a lipoprotein that binds multiple methionine analogs and plays dual roles in bacterial transport processes, tethering to membranes via a lipid anchor. It has been found to stimulate the ATPase activity of NmMetNI and may also have additional functions on the bacterial surface.
Article
Biochemistry & Molecular Biology
Rui Wang, Qiqin Xu, Chenlu Wang, Kai Tian, Hui Wang, Xiong Ji
Summary: Proteomic profiling identified ZBTB21 as a transcription factor that contributes to the chromatin binding and transcriptional repression of cohesin in 3D chromatin structures. Other ZBTB factors also interact with cohesin, and double degradation of ZBTB21 and ZBTB7B leads to a further decrease in cohesin chromatin occupancy.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Plant Sciences
Hui Feng, Tianli Liu, Jiaxu Li, Chuanxu Wan, Feifei Ding, Yuanchao Wang, Xiaobo Zheng, Wenwu Ye
Summary: In this study, a mutated version of the Phytophthora capsici oxysterol-binding protein-related protein 1 (PcMuORP1) was used as a selection marker for genetic transformation in Pythium ultimum. The knockout of PuM90 gene resulted in significant downregulation of PuLLP expression, and reintroduction of PuLLP using PcMuORP1 restored oospore production. PcMuORP1 showed higher screening efficiency and little long-term effect on fitness, making it a potential alternative selection marker. This study contributes to the functional genomic investigation of oomycetes.
PHYTOPATHOLOGY RESEARCH
(2023)
Article
Genetics & Heredity
Juan Pablo Gomez-Escribano, Lis Algora Gallardo, Kenan A. J. Bozhuyuk, Steven G. Kendrew, Benjamin D. Huckle, Nicola A. Crowhurst, Mervyn J. Bibb, Andrew J. Collis, Jason Micklefield, Paul R. Herron, Barrie Wilkinson
Summary: Streptomyces clavuligerus is an industrially important actinomycete with genetic manipulation limitations. Through Cas9-mediated genome editing, successful curing of pSCL4 revealed its crucial role in the structural stability of the linear chromosome.
MICROBIAL GENOMICS
(2021)
Article
Neurosciences
Savita G. Bhakta, James F. Cavanagh, Jo A. Talledo, Juliana E. Kotz, Lindsay Benster, Benjamin Z. Roberts, John A. Nungaray, Jonathan L. Brigman, Gregory A. Light, Neal R. Swerdlow, Jared W. Young
Summary: The poor translatability between preclinical and clinical drug trials has limited pro-cognitive therapeutic development. Future pro-cognitive drug trials should use translatable cross-species cognitive tasks with biomarkers relevant to specific cognitive constructs and sensitive to drug treatment. The study found that dextroamphetamine (d-amp) affected both behavioral and neurophysiological measures of cognitive control elements.
NEUROPSYCHOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
James E. Kath, Seungwoo Chang, Michelle K. Scotland, Johannes H. Wilbertz, Slobodan Jergic, Nicholas E. Dixon, Mark D. Sutton, Joseph J. Loparo
NUCLEIC ACIDS RESEARCH
(2016)
Article
Multidisciplinary Sciences
Elizabeth S. Thrall, James E. Kath, Seungwoo Chang, Joseph J. Loparo
NATURE COMMUNICATIONS
(2017)
Article
Multidisciplinary Sciences
Hua Bai, James E. Kath, Felix Manuel Zoergiebel, Mingxuan Sun, Pallavi Ghosh, Graham F. Hatfull, Nigel D. F. Grindley, John F. Marko
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2012)
Article
Multidisciplinary Sciences
James E. Kath, Slobodan Jergic, Justin M. H. Heltzel, Deena T. Jacob, Nicholas E. Dixon, Mark D. Sutton, Graham C. Walker, Joseph J. Loparo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Biochemical Research Methods
Jasmine M. Hershewe, William D. Wiseman, James E. Kath, Chelsea C. Buck, Maneesh K. Gupta, Patrick B. Dennis, Rajesh R. Naik, Michael C. Jewett
ACS SYNTHETIC BIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
James E. Kath, Aleksandra Baranczak
CHEMICAL COMMUNICATIONS
(2019)
Article
Biotechnology & Applied Microbiology
Jennifer A. Schoborg, Jasmine M. Hershewe, Jessica C. Stark, Weston Kightlinger, James E. Kath, Thapakorn Jaroentomeechai, Aravind Natarajan, Matthew P. DeLisa, Michael C. Jewett
BIOTECHNOLOGY AND BIOENGINEERING
(2018)