Article
Cell & Tissue Engineering
Cassandra M. Juran, Justina Zvirblyte, Eduardo A. C. Almeida
Summary: The forces generated by gravity have a significant impact on the development and gene expression of organisms. This study used single cell RNA-sequencing to explore the effects of hypergravity on developmental processes in embryonic stem cells and embryoid bodies. The results showed that gravity mechanostimulation can regulate the maintenance and development of these cells, and that cells committed to differentiation programs are more sensitive to gravity-induced changes in gene expression.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
Xiang Zhao, Bethany N. Radford, Mark Ungrin, Wendy Dean, Myriam Hemberger
Summary: Normal developmental progression relies on close interactions between the embryonic and extraembryonic lineages in the pre- and peri-gastrulation stage conceptus. Trophoblast cells of the extraembryonic ectoderm play a role in delaying cardiomyocyte differentiation dynamics and prolonging pluripotency in embryonic stem cells (ESCs). These findings provide insights into the intricate crosstalk between different compartments of the early conceptus and have implications for synthetic embryo research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Hisashi Yano, Takashi Inoue, Satoshi Murakami, Hiroyuki Kaneko
Summary: The aim of this study was to investigate the impact of oxygen tension on mouse induced pluripotent stem (iPS) cells in terms of differentiation and expression levels of collagen type 1 and Runx2. Under hypoxia, normoxia, and hyperoxia conditions, embryoid bodies (EBs) and outgrowth cells (OGCs) were cultured. In comparison to the hypoxia group, significantly larger EBs were observed in the hyperoxia and normoxia groups on Days 8 and 10. Furthermore, the hyperoxia group exhibited significantly higher expression levels of collagen type 1 and Runx2 mRNA and protein on Days 10 and 26, indicating accelerated differentiation of iPS cells.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Lu Qiao, So Hee Dho, Ji Young Kim, Lark Kyun Kim
Summary: SEPHS1 plays a minor role in maintaining pluripotency in mouse embryonic stem cells (ESCs), but is crucial for germ layer differentiation, especially for functional cardiac lineage.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Praphawi Nattasit, Kunimichi Niibe, Masahiro Yamada, Yumi Ohori-Morita, Phoonsuk Limraksasin, Watcharaphol Tiskratok, Masaya Yamamoto, Hiroshi Egusa
Summary: The stiffness of the substrate can regulate the behavior and differentiation of induced pluripotent stem cell (iPSC)-derived embryoid bodies (EB). A 3D hydrogel-sandwich culture (HGSC) system was developed to investigate the effects of mechanical cues on iPSC-EB differentiation. The moderate-stiffness HGSC promotes ectoderm and mesoderm lineage differentiation, as well as cardiomyocyte differentiation and myofibril structural maturation.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Cell & Tissue Engineering
Hanyi Zeng, Fanke Peng, Jiachen Wang, Ru Meng, Jun Zhang
Summary: The study showed that Fruquintinib can promote the maintenance of naive pluripotency and inhibit early differentiation programs in mESCs. Cells treated with Fruquintinib expressed higher levels of pluripotent markers under LIF condition, demonstrating its effect on mESC pluripotency.
CELLULAR REPROGRAMMING
(2021)
Article
Environmental Sciences
SunHwa Jeong, Changhwan Ahn, Jin-Sook Kwon, KangMin Kim, Eui-Bae Jeung
Summary: Inorganic arsenic is a known carcinogen and can disrupt the endocrine system even at low levels. This study investigated the effects of sodium arsenite (SA), an inorganic form of arsenic, on embryonic bodies (EBs) with three germ layers. The findings suggest that SA exposure inhibits the differentiation of EBs into cardiomyocytes and causes mitochondrial damage, interfering with myocardial differentiation and embryogenesis. The study highlights the need for increased awareness of SA exposure during pregnancy due to its irreversible adverse effects on embryogenesis through mitochondrial dysfunction.
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Cell Biology
Roni Sarel-Gallily, Tamar Golan-Lev, Atilgan Yilmaz, Ido Sagi, Nissim Benvenisty
Summary: This study identified haploinsufficiency-related genes and identified important dosage-dependent pathways involved in hESC growth and survival.
Article
Cell Biology
Simin Nafian Dehkordi, Farzaneh Khani, Seyedeh Nafiseh Hassani, Hossein Baharvand, Hamid Reza Soleimanpour-lichaei, Ghasem Hosseini Salekdeh
Summary: The study revealed a significant correlation between the upregulation of specific MSY genes and spontaneous differentiation, with most MSY genes being upregulated during differentiation and grouped into ectoderm, mesoderm, and endoderm clusters. These findings suggest a potential important role of MSY genes in early embryonic development.
Article
Cell Biology
German Atzin Mora-Roldan, Dalia Ramirez-Ramirez, Rosana Pelayo, Karlen Gazarian
Summary: This study compared the effects of 2D and 3D culture formats on the hematopoietic differentiation of human pluripotent stem cells, demonstrating that under the same conditions, the 3D format better supports the differentiation of stem cells into primitive hematopoietic cells, while the 2D format performs poorly.
Article
Biochemical Research Methods
Xue Han, Qi Zhang, Hui He, Qiang Zhao, Gang Li
Summary: A method for fabricating deep concave microwells for the large-scale production of uniform embryoid bodies (EBs) was developed. The method combined micromilling, caramel replica molding, and thermal reflow to create high aspect ratio micromolds. The deep concave microwell platform was shown to be practical for generating a large number of homogeneous EBs for potential tissue regeneration and cell therapy applications.
Article
Multidisciplinary Sciences
Veronica Veschi, Alice Turdo, Chiara Modica, Francesco Verona, Simone Di Franco, Miriam Gaggianesi, Elena Tirro, Sebastiano Di Bella, Melania Lo Iacono, Vincenzo Davide Pantina, Gaetana Porcelli, Laura Rosa Mangiapane, Paola Bianca, Aroldo Rizzo, Elisabetta Sciacca, Irene Pillitteri, Veronica Vella, Antonino Belfiore, Maria Rita Bongiorno, Giuseppe Pistone, Lorenzo Memeo, Lorenzo Colarossi, Dario Giuffrida, Cristina Colarossi, Paolo Vigneri, Matilde Todaro, Giorgio Stassi
Summary: In this study, the authors demonstrated the ability of hESCs to recapitulate different histotypes of thyroid carcinoma (TC) through specific genomic alterations using CRISPR-Cas9. They identified KISS1R and TIMP1 targeting as a potential therapeutic adjuvant option for undifferentiated TCs.
NATURE COMMUNICATIONS
(2023)
Article
Cell & Tissue Engineering
Yikai Huang, Ting Su, Congcong Wang, Lixia Dong, Shuang Liu, Yaru Zhu, Kunying Hao, Yin Xia, Qing Jiang, Jinzhong Qin
Summary: The deficiency of retinoblastoma binding protein 4 (RBBP4) in embryonic stem cells (ESCs) leads to spontaneous differentiation into mesendodermal lineages, and shares common genomic targets with the core Polycomb complex. RBBP4 is crucial for targeting PRC2 to a subset of developmental genes, and its presence is closely related to the maintenance of the pluripotency regulatory network and repression of mesendoderm lineages.
Article
Cell Biology
Mengtian Zhang, Libo Su, Wenwen Wang, Chenxiao Li, Qingli Liang, Fen Ji, Jianwei Jiao
Summary: The intimate communication between vascular and nervous systems is crucial for CNS development. In this study, it was found that during the development of vascular endothelial cells (ECs), DNA damage occurs. The E3 ubiquitin ligase RNF20 is required for the DNA damage response. Deletion of RNF20 leads to accumulation of DNA damage in ECs, impairing the secretion of cartilage intermediate layer protein 2 (CILP2). The loss of endothelium-derived CILP2 disrupts the downstream signaling of the Wnt pathway through interaction with Wnt3a, causing disturbances in NPC fate and autism-like behaviors in mice.
Article
Biochemistry & Molecular Biology
Ishaq A. Viringipurampeer, Andrew L. Metcalfe, Abu E. Bashar, Olena Sivak, Anat Yanai, Zeinabsadat Mohammadi, Orson L. Moritz, Cheryl Y. Gregory-Evans, Kevin Gregory-Evans
HUMAN MOLECULAR GENETICS
(2016)
Article
Cell & Tissue Engineering
Anat Yanai, Ishaq A. Viringipurampeer, Emran Bashar, Kevin Gregory-Evans
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2017)
Article
Materials Science, Multidisciplinary
T. N. Le, L. Straatman, A. Yanai, R. Rahmanian, C. Garnis, U. O. Hafeli, T. Poblete, B. D. Westerberg, K. Gregory-Evans
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
(2017)
Article
Medicine, Research & Experimental
Xia Wang, Kevin Gregory-Evans, Kishor M. Wasan, Olena Sivak, Xianghong Shan, Cheryl Y. Gregory-Evans
MOLECULAR THERAPY-NUCLEIC ACIDS
(2017)
Article
Neurosciences
Ishaq A. Viringipurampeer, Cheryl Y. Gregory-Evans, Andrew L. Metcalfe, Emran Bashar, Orson L. Moritz, Kevin Gregory-Evans
MOLECULAR NEUROBIOLOGY
(2019)
Review
Cell & Tissue Engineering
Emily Young, Brian Westerberg, Anat Yanai, Kevin Gregory-Evans
REGENERATIVE MEDICINE
(2018)
Article
Cell & Tissue Engineering
Ishaq A. Viringipurampeer, Anat Yanai, Vahitha S. Nizamudheen, Cheryl Y. Gregory-Evans, Kevin Gregory-Evans
Summary: Cell replacement therapy holds promise for treating neurodegenerative diseases, but challenges remain in improving cell survival and integration. Pre-treatment with PKX-001 enhances cell survival, maturation, and functional integration in vivo, showing potential for clinical application in repairing damaged retinas.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Allison J. Selinger, Natalie A. Cavallin, Anat Yanai, Inanc Birol, Fraser Hof
Summary: This study reports the synthesis of bivalent water-soluble calix[4]arene and calix[5]arene hosts, Super-sCx4 and Super-sCx5, as new broad-spectrum supramolecular binders of neuromuscular blocking agents. The hosts bind a variety of NMBAs by engaging both quaternary amines present, with high selectivity over other hydrophobic amines. This binding is achieved through linking two highly anionic p-sulfonatocalixarene building blocks in aqueous solution using the target bisquaternary amine NMBAs as a template.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Infectious Diseases
Diana Lin, Darcy Sutherland, Sambina Islam Aninta, Nathan Louie, Ka Ming Nip, Chenkai Li, Anat Yanai, Lauren Coombe, Rene L. Warren, Caren C. Helbing, Linda M. N. Hoang, Inanc Birol
Summary: Antibiotic resistance is a global health crisis, and alternative antimicrobial therapeutics are urgently needed. This study presents rAMPage, a bioinformatics discovery platform, to identify AMP sequences from RNA-seq datasets. The researchers used rAMPage on 84 publicly available RNA-seq datasets, identifying 1137 possible AMPs, with 1024 deemed novel. Testing 21 selected peptide sequences, seven of them showed high antimicrobial activity.
Review
Ophthalmology
Anat Yanai, Pia McNab, Kevin Gregory-Evans
EXPERT REVIEW OF OPHTHALMOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Abu E. Bashar, Andrew L. Metcalfe, Ishaq A. Viringipurampeer, Anat Yanai, Cheryl Y. Gregory-Evans, Kevin Gregory-Evans