Article
Medicine, Research & Experimental
Chushan Zheng, Zehong Yang, Shihao Chen, Fang Zhang, Zilong Rao, Cailing Zhao, Daping Quan, Ying Bai, Jun Shen
Summary: NGCs composed of longitudinally aligned electrospun nanofibers and 0.25% pDNM gel show promising outcomes in directing axonal extension, promoting nerve fiber myelination, and facilitating functional recovery. Furthermore, T2-mapping and DTI metrics are found to be useful non-invasive monitoring techniques for PNI treatment.
Review
Biotechnology & Applied Microbiology
Ming-Wei Hsu, Szu-Han Chen, Wan-Ling Tseng, Kuo-Shu Hung, Tzu-Chun Chung, Sheng-Che Lin, Jahyun Koo, Yuan-Yu Hsueh
Summary: In severe or complex peripheral nerve injuries, autologous nerve grafts have promising results but limited availability and donor site morbidity. Biological or synthetic substitutes are inconsistent, while biomimetic alternatives from allogenic or xenogenic sources offer an off-the-shelf supply. Successful peripheral nerve regeneration requires effective decellularization, which can be achieved through physical methods.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yan Kong, Jiawei Xu, Qi Han, Tiantian Zheng, Linliang Wu, Guicai Li, Yumin Yang
Summary: In this study, a pure porcine decellularized nerve matrix conduit (xDNME) was successfully developed using an electrospinning technique without any additives. The xDNME conduit exhibited an obvious fiber structure and retained the natural components of the decellularized nerve tissue. In vivo experiments showed that xDNME significantly promoted the regeneration of rat sciatic nerve and exhibited comparable myelin sheath thickness and electrical signal transmission ability to the autologous nerve.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Nanoscience & Nanotechnology
Wen Xue, Yunfan Kong, Rafay Abu, Pooja Roy, Sung-Ho Huh, Mitchell Kuss, Vikas Kumar, Bin Duan
Summary: Decellularized nerve hydrogels (dNHs) have shown promising effects on promoting Schwann cell proliferation and neurite outgrowth. The mechanics and components of the hydrogel contribute to its functionality, with laminin (LAM) and type V collagen (COLV) playing important roles. Additionally, by adding LAM and COLV to collagen hydrogels, gene expression of Schwann cells can be regulated, leading to improved spreading and neurite outgrowth. Furthermore, dNHs can be fabricated into paper-like, aligned nerve scaffolds for peripheral nerve injury treatment.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Tomasz Debski, Ewa Kijenska-Gawronska, Aleksandra Zolocinska, Katarzyna Siennicka, Anna Slysz, Wiktor Paskal, Pawel K. Wlodarski, Wojciech Swieszkowski, Zygmunt Pojda
Summary: The study aimed to examine the efficiency of a scaffold with adipose-derived stem cells as a nerve conduit in a rat model, showing promising results in managing nerve gap by reducing muscle atrophy and improving nerve fiber density.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Joo-Yul Bae, Suk Young Park, Young Ho Shin, Shin Woo Choi, Jae Kwang Kim
Summary: Animal studies have shown that using amphoteric detergent and nuclease is the most reliable method for decellularizing peripheral nerves. This method successfully removes cellular components and preserves extracellular matrix integrity, outperforming nonionic and anionic detergents. Additionally, the decellularized nerves are biocompatible and maintain comparable biomechanical properties to fresh controls.
NEURAL REGENERATION RESEARCH
(2021)
Article
Health Care Sciences & Services
Arne Boecker, Martin Aman, Ulrich Kneser, Leila Harhaus, Frank Siemers, Felix Stang
Summary: The efficacy of chitosan-based nerve conduits in regenerating sensory nerves in the hand was evaluated in this study. The results showed that this type of nerve conduit had similar functional outcomes and patient satisfaction to autologous nerve grafts in treating small nerve defects.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Oscar Dario Garcia-Garcia, Marwa El Soury, David Gonzalez-Quevedo, David Sanchez-Porras, Jesus Chato-Astrain, Fernando Campos, Victor Carriel
Summary: Acellular nerve allografts (ANGs) have shown to be a promising alternative in nerve repair. This study demonstrates that using genipin (GP) as a crosslinking agent can improve the biomechanical properties of ANGs, especially in RS and RS-GP 0.10% ANGs, with minimal impact on biocompatibility and histological pattern. This novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Enxing Yu, Zhiwu Chen, Yuye Huang, Yibing Wu, Zonghuan Wang, Fangfang Wang, Miaoben Wu, Kailei Xu, Wei Peng
Summary: Peripheral nerve injury (PNI) is a common and severe clinical disease with poor prognosis. Autologous nerve grafting is limited in availability and size, leading to the development of artificial nerve conduits. In this study, a novel composite neural conduit containing decellularized tissues and a grooved PLGA conduit was found to significantly promote nerve regeneration. This artificial nerve conduit could potentially be used in clinical practice.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2023)
Review
Neurosciences
Ting Li, Rabia Javed, Qiang Ao
Summary: This review discusses the utilization of xenogeneic decellularized extracellular matrix (dECM) based biomaterials for surgical nerve repair. It highlights the potential of dECM as a reliable alternative for promoting nerve regeneration with a variety of applications.
CURRENT NEUROPHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Steven Zhou, Nasir Hussain, Alaa Abd-Elsayed, Racha Boulos, Mohammed Hakim, Mayank Gupta, Tristan Weaver
Summary: Headaches are a common medical complaint worldwide, with peripheral nerve stimulation emerging as a novel treatment for intractable headaches. While evidence supports its use, efficacy is limited to specific nerves and headache types, requiring further research for mainstream adoption.
Review
Biochemistry & Molecular Biology
Takehiro Kawashiri, Keisuke Mine, Daisuke Kobayashi, Mizuki Inoue, Soichiro Ushio, Mayako Uchida, Nobuaki Egashira, Takao Shimazoe
Summary: Oxaliplatin is an essential drug in chemotherapy for colorectal, gastric, and pancreatic cancers, but it often causes peripheral neuropathy. Basic research has identified many potential neuropathy inhibitors, but few drugs have demonstrated clear efficacy in clinical trials for oxaliplatin-induced peripheral neuropathy. It is crucial to further translational research to bridge the gap between basic research and clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Kevin J. Zuo, Golsa Shafa, Katelyn Chan, Jennifer Zhang, Cynthia Hawkins, Kasra Tajdaran, Tessa Gordon, Gregory H. Borschel
Summary: The study showed that local FK506 DDS can eliminate the toxicity of systemic immunosuppressant FK506 by enhancing motor and sensory nerve regeneration through nerve allografts. This treatment may have potential clinical applications in peripheral nerve reconstruction or vascularized composite allotransplantation.
EXPERIMENTAL NEUROLOGY
(2021)
Review
Cell Biology
Kelly C. S. Roballo, Jason P. Gigley, Tyler A. Smith, George D. Bittner, Jared S. Bushman
Summary: This review discusses the unique functional and immunological properties of live allogeneic peripheral nerves compared to other transplanted tissues. It highlights the effectiveness of live peripheral nerve allografts in promoting recovery after peripheral nerve injury and the potential interest of understanding their immunological peculiarities in the field of transplantation. The review addresses topics such as the utility of peripheral nerve allografts in bridging nerve defects, the host immune response to these grafts, and the potential mechanisms accounting for immunological differences.
NEURAL REGENERATION RESEARCH
(2022)
Article
Cell Biology
Estefania Contreras, Sara Bolivar, Nuria Nieto-Nicolau, Oscar Farinas, Patricia Lopez-Chicon, Xavier Navarro, Esther Udina
Summary: This study assessed the regenerative potential of decellularized nerve allografts compared to autografts for repairing nerve resections. The results showed that while decellularized allografts sustained regeneration in rats, regeneration was limited with decellularized xenografts.
CELL AND TISSUE RESEARCH
(2022)
Article
Biochemical Research Methods
Deng Pan, Junichi Sayanagi, Jesus A. Acevedo-Cintron, Lauren Schellhardt, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: This study introduced a new method of embedding liposomes within fibrin gels to target macrophages at specific spatial locations in nerve regeneration. The clodronate liposomes showed specific toxicity to macrophages in vitro and effectively depleted macrophages in vivo without affecting other cell types. The delivery system allowed for sustained release of liposomes and demonstrated promising results for nerve regeneration in mice.
JOURNAL OF NEUROSCIENCE METHODS
(2021)
Article
Neurosciences
Deng Pan, Lauren Schellhardt, Jesus A. Acevedo-Cintron, Daniel Hunter, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: IL-4 plays a crucial role in nerve regeneration, promoting axonal extension and recovery after injury. Loss of IL-4 affects macrophage accumulation and delays axon regeneration, leading to delayed functional recovery. Exogenous IL-4 administration enhances early axonal extension and macrophage accumulation, but does not impact functional recovery compared to untreated mice.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Surgery
Joseph Roh, Lauren Schellhardt, Grace C. Keane, Daniel A. Hunter, Amy M. Moore, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: This study found that as little as 10 minutes of intraoperative electrical stimulation therapy increased early axon regeneration and facilitated functional recovery following nerve transection with repair. These findings provide a new strategy to improve nerve injury repair outcomes.
PLASTIC AND RECONSTRUCTIVE SURGERY
(2022)
Article
Ophthalmology
Jyh Haur Woo, Simeon Christian Daeschler, Kamiar Mireskandari, Gregory Howard Borschel, Asim Ali
Summary: The purpose of this retrospective case series was to measure sensory recovery after minimally invasive corneal neurotization and investigate the influence of patient and technical factors on sensory recovery, ulceration rate, and visual outcomes. The study included 23 patients who underwent indirect corneal neurotization for neurotrophic keratopathy. The results showed that corneal boundary improvements were significantly correlated with time after surgery, choice of donor nerve, and the number of fascicles and insertions.
AMERICAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Medicine, Research & Experimental
Jackson S. Burton, Arthur C. Sletten, Evan Marsh, Matthew D. Wood, Justin M. Sacks
Summary: Lymphedema is a chronic condition characterized by impaired lymphatic flow, leading to limb swelling and debilitation. It primarily affects cancer survivors, with obesity and body mass index being independent risk factors. Current treatments focus on palliative approaches and surgery, but there is a need for novel therapeutic strategies that target the role of adipose tissue in lymphedema progression. Animal models have suggested the potential of targeting adipocyte metabolism as a new experimental treatment approach.
LYMPHATIC RESEARCH AND BIOLOGY
(2023)
Article
Biochemical Research Methods
Igor D. Luzhansky, Emma Anisman, Dharma Patel, Naasik Syed, Matthew D. Wood, Mikhail Y. Berezin
Summary: This study demonstrated the feasibility of monitoring fibrin dynamics using nerve guidance conduits (NGCs) containing fluorescently tagged fibrin. Visualizing the in vivo intraneural fibrin degradation provides a potential indicator of regeneration success. The results showed that fluorescent ECM, in this case fibrin, can facilitate imaging of regeneration in peripheral nerve conduits without significantly affecting the regeneration process.
JOURNAL OF BIOMEDICAL OPTICS
(2022)
Article
Surgery
Patrick D. Grimm, Benjamin M. Wheatley, Allison Tomasino, Crystal Leonhardt, Daniel A. Hunter, Matthew D. Wood, Amy M. Moore, Thomas A. Davis, Scott M. Tintle
Summary: The study demonstrated the ability of a long acellular nerve allograft to prevent neuroma formation in a swine model of nerve injury, showing a decreased number of axons and reduced cross sectional area of nerve tissue in the treatment group.
Article
Engineering, Biomedical
Anne E. Faust, Lorenzo Soletti, Nicole A. Cwalina, Andrew D. Miller, Matthew D. Wood, Mark A. Mahan, Jonathan Cheetham, Bryan N. Brown
Summary: This study describes the development and testing of a xenogeneic nerve cap graft made from decellularized porcine nerve. The grafts showed promising results in in vitro and in vivo testing, providing proof of concept for a novel xenograft-based approach to neuroma prevention.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Clinical Neurology
Evan B. Marsh, Lauren Schellhardt, Daniel A. A. Hunter, Susan E. E. Mackinnon, Alison K. K. Snyder-Warwick, Matthew D. D. Wood
Summary: The dual administration of FK506 and E-stim does not have significant additive effects on regeneration and recovery compared to either therapy alone. The combination of FK506 and E-stim appears to combine the relative strengths of each therapeutic.
Article
Neurosciences
Adam Liebendorfer, Michael J. Finnan, Jonathon Blake Schofield, Sai L. Pinni, Jesus A. Acevedo-Cintron, Lauren Schellhardt, Alison K. Snyder-Warwick, Susan E. Mackinnon, Matthew D. Wood
Summary: This research shows that eosinophils play a significant role in nerve regeneration by promoting axon regeneration and regulating cytokines that influence macrophage phenotypes. However, eosinophils are not essential for nerve regeneration.
EXPERIMENTAL NEUROLOGY
(2023)
Review
Clinical Neurology
M. Jakeman, G. H. Borschel, P. Sharma
Summary: C7 nerve transfer in BPBI has few long-term donor limb complications, with transient sensory and motor deficits. The impact on upper limb function in this patient cohort is still unknown.
CHILDS NERVOUS SYSTEM
(2023)
Article
Clinical Neurology
Deng Pan, Jonathon Blake Schofield, Lauren Schellhardt, Alison K. Snyder-Warwick, Susan E. Mackinnon, Xiaowei Li, Matthew D. Wood
Summary: This study evaluated the feasibility and effects of transplanting exogenous macrophages to a segmental nerve injury. The results showed that incorporating macrophage colony-stimulating factor (M-CSF) improved the survival and expansion of bone marrow-derived macrophages (BMDM). Transplanted BMDM survived for at least 7 days in the nerve gap and promoted angiogenesis and axonal extension. The findings suggest that transplanting exogenous macrophages could be a beneficial strategy for promoting regeneration after nerve injury.
Article
Orthopedics
Adam Evans, William M. Padovano, J. Megan M. Patterson, Matthew D. Wood, Warangkana Fongsri, Carie R. Kennedy, Susan E. Mackinnon
Summary: This study evaluated the impact of adjunctive procedures and electrical stimulation on the outcomes of patients with cubital tunnel syndrome. The results showed that patients who underwent adjunctive procedures had greater improvement in DASH scores, and patients who received electrical stimulation also had significant improvement in DASH scores.
HAND-AMERICAN ASSOCIATION FOR HAND SURGERY
(2023)
Article
Orthopedics
Blair R. Peters, Matthew D. Wood, Daniel A. Hunter, Susan E. Mackinnon
Summary: This article reports five cases of nerve injuries repaired with acellular nerve allografts and highlights the limitations of this method through histomorphological analysis. The authors suggest careful consideration of the use of acellular nerve allografts in the repair of long length, large-diameter nerve injuries.
HAND-AMERICAN ASSOCIATION FOR HAND SURGERY
(2023)
Review
Clinical Neurology
John M. Daines, Lauren Schellhardt, Matthew D. Wood
Summary: IL-4 signaling plays a crucial role in nerve injury and regeneration, promoting neuron survival, axonal regeneration, and remyelination for improved functional recovery. Targeting the IL-4 signaling pathway shows promise for treating traumatic nerve injury, but a better understanding of the immune response and signaling networks within the nerve injury microenvironment is still needed.
NEUROREHABILITATION AND NEURAL REPAIR
(2021)