Article
Chemistry, Multidisciplinary
Sheng-Long Ding, Xi-Yuan Zhao, Wei Xiong, Lin-Feng Ji, Min-Xuan Jia, Yan-Yan Liu, Hai-Tao Guo, Feng Qu, Wenguo Cui, Qi Gu, Ming-Zhu Zhang
Summary: Cartilage equivalents from hydrogels containing chondrocytes show great potential in hyaline cartilage regeneration. However, current methods struggle to recreate the original architecture and culture non-differentiated chondrocytes in vitro. This study introduces specially designed microcarriers that use mechanotransductive conditions to form stable hyaluronic acid, contributing to the regeneration of hyaline cartilaginous microtissue and preventing chondrocyte dedifferentiation.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Sheng-Long Ding, Xin Liu, Xi-Yuan Zhao, Ke-Tao Wang, Wei Xiong, Zi-Li Gao, Cheng-Yi Sun, Min-Xuan Jia, Cheng Li, Qi Gu, Ming-Zhu Zhang
Summary: The successful regeneration of cartilage tissue at a clinical scale has been a significant challenge. Microcarrier systems offer an attractive approach to regulate cell phenotype and tissue maturation, and have shown promise in cartilage regeneration. This review provides comprehensive guidelines for the rational design and applications of microcarriers in cartilage tissue engineering.
BIOACTIVE MATERIALS
(2022)
Review
Orthopedics
Sida Liao, Haoye Meng, Junkang Li, Jun Zhao, Yichi Xu, Aiyuan Wang, Wenjing Xu, Jiang Peng, Shibi Lu
Summary: The application of microcarriers in cartilage regeneration has advanced significantly in recent years, providing a conducive microenvironment for cell expansion and enabling the delivery of proteins, factors, and drugs. They offer advantages like injectability and targeted delivery, overcoming limitations of traditional methods and enhancing efficiency and mechanical properties in combination with other stents.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2021)
Article
Biotechnology & Applied Microbiology
Pedro Silva Couto, Dale J. J. Stibbs, Marco C. C. Rotondi, Yasuhiro Takeuchi, Qasim A. A. Rafiq
Summary: This study demonstrates a scalable manufacturing process using a microcarrier-bioreactor system for the expansion of gene-modified human mesenchymal stromal cells (hMSCs). The transduced and expanded cells met the criteria established by the International Society for Cell and Gene Therapy (ISCT). This work provides a foundation for the scalable manufacturing of gene-modified hMSCs.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Maurizio Marzaro, Gianantonio Pozzato, Stefano Tedesco, Mattia Algeri, Alessandro Pozzato, Luigi Tomao, Ilaria Montano, Filippo Torroni, Valerio Balassone, Anna Chiara Iolanda Contini, Luciano Guerra, Tommaso D'Angelo, Giovanni Federici di Abriola, Lorenzo Lupoi, Maria Emiliana Caristo, Ivo Boskoski, Guido Costamagna, Paola Francalanci, Giuseppe Astori, Angela Bozza, Andrea Bagno, Martina Todesco, Emanuele Trovalusci, Luigi Dall' Oglio, Franco Locatelli, Tamara Caldaro
Summary: Current surgical options for esophageal replacement have limitations in guaranteeing satisfactory quality of life for patients. Tissue engineering techniques, such as seeding autologous cells on scaffolds, offer a promising solution by simplifying surgical procedures and achieving good clinical outcomes. The use of Quantum Molecular Resonance technology to create microchannels within the decellularized esophageal scaffold improves cell distribution and promotes esophageal regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Materials Science, Multidisciplinary
Ahmad Moukachar, Katie Harvey, Eva Roke, Katherine Sloan, Cameron Pool, Shabbir Moola, Ameer Alshukri, Danielle Jarvis, Phoebe Crews-Rees, Grace McDermott, Lluan Evans, Jin Li, Christopher Thomas, Sion Coulman, Oliver Castell
Summary: The development of low-cost accessible technologies for rapid prototyping of mechanical components has made engineering tools more accessible to hobbyists and researchers. A similar approach can be applied to the fabrication of soft-matter and biologically compatible materials, enabling advancements in biological research.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Biotechnology & Applied Microbiology
Nadine Frerker, Tommy A. A. Karlsen, Maria Stensland, Tuula A. A. Nyman, Simon Rayner, Jan E. E. Brinchmann
Summary: Focal lesions of articular cartilage can cause pain and reduced joint function, leading to osteoarthritis if untreated. In vitro generated, scaffold-free autologous cartilage discs made from articular chondrocytes were compared to those made from bone marrow-derived mesenchymal stromal cells. Articular chondrocytes produced more extracellular matrix and contained more articular cartilage proteins, while mesenchymal stromal cells had more proteins associated with cartilage hypertrophy and bone formation. MicroRNA analysis suggested that differential expression of microRNAs played a key role in the differential synthesis of proteins. Therefore, articular chondrocytes are preferred for engineering articular cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Md. Shafiullah Shajib, Kathryn Futrega, Rose Ann G. Franco, Eamonn McKenna, Bianca Guillesser, Travis J. Klein, Ross W. Crawford, Michael R. Doran
Summary: This study developed a method for the mass production and cryopreservation of 3D cartilage microtissues, and found that the cryopreserved microtissues maintained their tissue function.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Po-Chih Shen, Cheng-Chang Lu, Shih-Hsiang Chou, Zi-Miao Liu, Shu-Jem Su, Yin-Chun Tien
Summary: This study compared stratified chondrocyte sheets (S-CS) and heterogeneous chondrocyte sheets (H-CS) in terms of expression and therapeutic effects, revealing that the S-CS group exhibited higher cell viability, proliferation rate, expression of chondrogenic markers, secretion of tissue inhibitor of metalloproteinase, and GAG production level. The mini-pigs articular cartilage defect model showed that the S-CS group had a higher International Cartilage Repair Society (ICRS) macroscopic score and displayed a zonal structure closer to native cartilage.
Article
Orthopedics
Yuxin Yan, Rao Fu, Chuanqi Liu, Jing Yang, Qingfeng Li, Ru-Lin Huang
Summary: A sequential chondrocyte isolation protocol was developed with higher cell-yield efficiency and better chondrocyte quality compared to the classic protocol. Cells harvested after sequential digestions demonstrated higher viability, proliferative activity, chondrocyte phenotype, and cartilage-specific matrix synthesis ability.
Article
Materials Science, Biomaterials
Daiying Song, Mengyuan Yu, Jingwen Liu, Wei Xu, Juncen Li, Baihui Li, Yilin Cao, Guangdong Zhou, Yujie Hua, Yu Liu
Summary: By using cartilage regeneration units (CRUs) based on hydrogel microcarriers, injectable cartilage regeneration in goats was successfully achieved, resulting in relatively mature cartilage microtissue. This method improves the utilization rate of the culture space and provides support for future clinical applications.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Chemistry, Applied
Duc Dung Nguyen, Chun-Hsu Yao, Li-Jyuan Luo, Hung-Chi Chen, Yi-Jen Hsueh, David Hui-Kang Ma, Jui-Yang Lai
Summary: This study explores the effects of oxidation level on gelatin microcarriers for repairing corneal injuries, and finds that high oxidation levels can enhance the adhesion and growth of keratocytes. In a rabbit model of corneal alkali burn injury, the use of microcarriers with appropriate oxidation level effectively reduces corneal swelling, promotes collagen production and keratocan expression, and repairs the collagenous stromal architecture.
CARBOHYDRATE POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Panneerselvam Jithendra, Jamal Moideen Muthu Mohamed, Dinesh Annamalai, Rasha Hamed Al-Serwi, Ateya Megahed Ibrahim, Mohamed El-Sherbiny, Abraham Merlin Rajam, Mamdouh Eldesoqui, Nimer Mansour
Summary: The chondrogenic efficacy of aloe vera blended collagen-chitosan (COL-CS-AV) porous scaffold was investigated using articular chondrocytes. The scaffold showed higher chondrogenic efficacy compared to COL-CS, making it a suitable candidate for cartilage tissue engineering. The study utilized various assays and staining techniques to analyze cytocompatibility, GAG content, RNA content, and expression of cartilage-specific genes and collagen type II.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Maria Oliver-Ferrandiz, Lara Milian, Maria Sancho-Tello, Jose Javier Martin de Llano, Fernando Gisbert Roca, Cristina Martinez-Ramos, Carmen Carda, Manuel Mata
Summary: A novel MACI scaffold composed of a mixed alginate-agarose hydrogel with human dental pulp stem cells (hDPSCs) was proposed, showing superior biomechanical properties and uniform cell distribution. The hDPSCs cultured in the scaffold exhibited good chondrogenic differentiation potential.
Article
Polymer Science
Mike Wagenbrenner, Susanne Mayer-Wagner, Maximilian Rudert, Boris Michael Holzapfel, Manuel Weissenberger
Summary: Cartilage has limited regenerative capacity, and cell-based approaches are a promising alternative for the treatment of cartilage defects and osteoarthritis, with MSCs being an attractive cell source often combined with hydrogels. This review focused on the advantages and disadvantages of various hydrogels, as well as different combinations of MSC subpopulations and hydrogels used in cartilage engineering.
Article
Chemistry, Multidisciplinary
Maurice C. E. van Dalen, Jonathan Vaneyck, Slav A. Semerdzhiev, Marcel Karperien, Janine N. Post, Mireille M. A. E. Claessens
Summary: The viscoelastic response of amyloid fibril networks of lysozyme protein in biologically relevant solutions differs significantly from their response in simple buffers, with enhanced energy dissipation observed in cell culture medium and synovial fluid. This energy dissipation is attributed to interactions of amyloid fibrils with other molecules in these solutions, particularly the adsorption of serum albumin protein. This finding provides a basis for a better understanding of the performance of amyloid hydrogels in biomedical applications.
Review
Rheumatology
Carlo Alberto Paggi, Liliana Moreira Teixeira, Severine Le Gac, Marcel Karperien
Summary: Arthritis is a global health problem affecting millions of people, with limited treatment options. Current disease models for drug screening and development do not accurately replicate all aspects of human joint diseases. A humanized joint-on-chip (JoC) model has the potential to revolutionize research and drug development in rheumatic diseases.
NATURE REVIEWS RHEUMATOLOGY
(2022)
Article
Immunology
Jan Hendriks, Richard Schasfoort, Michelle Koerselman, Maureen Dannenberg, Alexander Daniel Cornet, Albertus Beishuizen, Job van der Palen, Johannes Krabbe, Alide H. L. Mulder, Marcel Karperien
Summary: This study investigates the humoral antibody response in COVID-19 patients and its impact on disease progression. The findings suggest that antibody titers are positively associated with disease severity, while binding strength decreases with increasing severity. In critically ill patients, there is also a positive association between antibody titers and symptoms such as pulmonary embolism.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Polymer Science
Yao Fu, Sanne K. Both, Jacqueline R. M. Plass, Pieter J. Dijkstra, Bram Zoetebier, Marcel Karperien
Summary: This study demonstrates that hydrogels laden with different types of cells result in distinct host responses. Hydrogels laden with hMSCs or bCHs showed beneficial interaction in terms of enhanced cell proliferation and matrix deposition. Host gender has an impact on the performance of hydrogels laden with bCHs.
Article
Biochemical Research Methods
Jan Hendriks, Richard B. M. Schasfoort, Jurriaan Huskens, DanielB. F. Saris, Marcel Karperien
Summary: This paper presents a toolbox for kinetically characterizing biomarker measurement assays using SPRi. The toolbox allows for real-time quality control and accurate prediction of measurements at low and high concentrations of analytes. The biphasic binding sites model accurately fits experimental curves and enables optimal detection of antibodies, cross-reactivity, spotting irregularities, and other confounders. The toolbox can also create calibration curves for calibration-free measurements and aid in assay optimization for clinical translation of new biomarker assays.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Syed Ahmed Shah, Muhammad Sohail, Marcel Karperien, Castro Johnbosco, Arshad Mahmood, Mubeen Kousar
Summary: Biopolymer-based thermoresponsive injectable hydrogels containing multifunctional characteristics show great potential for diabetic wound regeneration. In this study, Chitosan-CMC-g-PF127 injectable hydrogels loaded with Curcumin were developed, which exhibited viscoelastic behavior, good swelling properties, and a controlled release profile. The hydrogels demonstrated good biocompatibility and tissue regenerative ability, making them a promising platform for sustained drug delivery.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Tom Kamperman, Niels G. A. Willemen, Cindy Kelder, Michelle Koerselman, Malin Becker, Luanda Lins, Castro Johnbosco, Marcel Karperien, Jeroen Leijten
Summary: This study demonstrates that stimuli-responsive cell-adhesive micromaterials can self-assemble with cells into 3D living composite microtissues and provide control over the biomechanical and biochemical environment, influencing cell behavior.
Article
Engineering, Biomedical
Rong Wang, Xiaobin Huang, Bram Zoetebier, Pieter J. Dijkstra, Marcel Karperien
Summary: In this study, hydrogels with adjustable elasticity and degradation time were designed using the properties of hyaluronic acid and star shaped poly(ethylene glycol). The hydrogels showed biocompatibility and enhanced production of collagen type II, making them suitable as scaffolds for tissue regeneration applications.
BIOACTIVE MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Francesca Signori, Jos W. H. Wennink, Simona Bronco, Jan Feijen, Marcel Karperien, Ranieri Bizzarri, Pieter J. Dijkstra
Summary: Poly(lactide) (PLA) and poly(ethylene glycol) (PEG)-based hydrogels were prepared by mixing enantiomerically pure copolymers with opposite chirality. The gelation process was found to follow different mechanisms depending on the nature of the linker R. Mixing equimolar amounts of the enantiomeric copolymers resulted in micellar aggregates with a stereocomplexed PLA core and a hydrophilic PEG corona.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Maurice C. E. van Dalen, Marcel Karperien, Mireille M. A. E. Claessens, Janine N. Post
Summary: The study investigates the use of amyloid fibril-based scaffolds to stimulate chondrocytes for the formation of articular cartilage. Different proteins were induced to self-assemble into amyloid fibrils and form micron scale amyloid networks resembling cartilage extracellular matrix. The results show that lysozyme amyloid micronetworks support chondrocyte viability and extracellular matrix deposition, while alpha-synuclein and beta-lactoglobulin only maintain cell viability.
Review
Engineering, Biomedical
Michelle Koerselman, Lisanne C. M. Morshuis, Marcel Karperien
Summary: This article discusses the application of small molecules such as peptides, aptamers, and variable antigen-binding domain of heavy chain only antibodies in tissue engineering and regenerative medicine. It compares these biological functionalities with conventional antibodies and provides an overview of recent work in this area. The article focuses on the structural differences, modifications, and conjugation strategies of these biologicals, as well as the clinical trials involving them. The challenges associated with each of these biologicals are also highlighted.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Nuno Araujo-Gomes, Barbara Zoetebier-Liszka, Bas van Loo, Malin Becker, Suzanne Nijhuis, Alexandra M. Smink, Bart J. de Haan, Paul de Vos, Marcel Karperien, Jeroen Leijten
Summary: Transplantation of microencapsulated pancreatic cells using monodisperse PEG-TA microgels is a promising therapy for T1DM. The microgels provide immunoprotection to transplanted cells and maintain their viability and function. These microencapsulated beta-cells are able to rapidly self-assemble into islet-sized spheroids, show limited immunogenicity, and remain glucose responsive both in vitro and in vivo.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Engineering, Biomedical
Castro Johnbosco, Lisanne Karbaat, Nicoline M. Korthagen, Kelly Warmink, Michelle Koerselman, Katja Coeleveld, Malin Becker, Bas van Loo, Bram Zoetebier, Sanne Both, Harrie Weinans, Marcel Karperien, Jeroen Leijten
Summary: This study investigates the effect of micromaterial composition and concentration on the therapeutic outcome of intra-articular injections of microencapsulated stem cells for the treatment of osteoarthritis. The results demonstrate that different polymer compositions and concentrations can significantly alter the metabolic activity and secretome of MSCs, as well as the retention time and biodistribution within the joint. Furthermore, the study shows that the different microgel formulations have a material-dependent effect on reducing cartilage degradation and matrix loss in rat joints with osteoarthritis.
MATERIALS TODAY BIO
(2023)
Article
Biochemical Research Methods
Carlo Alberto Paggi, Jan Hendriks, Marcel Karperien, Severine Le Gac
Summary: In this study, the effects of different types of mechanical stimulation on chondrocyte phenotype and extracellular matrix (ECM) production were investigated using a cartilage-on-chip system. The results showed that mechanical stimulation enhanced matrix protein expression and promoted the production of cartilage ECM, with the most significant effects observed with multi-directional stimulation.
Meeting Abstract
Orthopedics
L. Zhong, M. Koerselman, L. Karbaat, T. Kamperman, J. Leijten, B. Zoetebier, M. Karperien
OSTEOARTHRITIS AND CARTILAGE
(2021)
