Article
Cell & Tissue Engineering
Pei Shen, Lu Chen, Dahe Zhang, Simo Xia, Zhuman Lv, Duohong Zou, Zhiyuan Zhang, Chi Yang, Wenlin Li
Summary: Ectodermal chondrogenic cells (ECCs) with self-renewal ability were generated from human pluripotent stem cells (hPSCs) through the neural crest (NC). ECCs had similar transcriptome profiles to condylar chondrocytes and could form cartilage-like structures resembling mandibular condylar cartilage in vivo.
NPJ REGENERATIVE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Hui Song Cui, So Young Joo, Seung Yeol Lee, Yoon Soo Cho, Dong Hyun Kim, Cheong Hoon Seo
Summary: It is unclear how scar fibroblasts (SFs) affect keratinocytes in hypertrophic scars (HTS) through cell-cell interaction. This study investigated the effects of HTS-derived exosomes on the proliferation and differentiation of normal human keratinocytes (NHKs) and compared them with normal fibroblasts (NFs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Luminita Labusca, Camelia Danceanu, Anca Emanuela Minuti, Dumitru-Daniel Herea, Adrian Ghemes, Cristian Rotarescu, Oana Dragos-Pinzaru, Mihai Tibu, Grigoras Marian, Horia Chiriac, Nicoleta Lupu
Summary: Magnetic nanomaterials have a growing impact in the field of biology and medicine due to their versatility in drug delivery, cell targeting, and imaging. This study investigates the use of uncoated nickel nanowires as a mechanically-responsive substrate for inducing osteogenic differentiation of adipose derived-mesenchymal cells (ASCs) through magneto-mechanical stimulation. Preliminary results show a significant increase in osteogenic differentiation of ASCs exposed to a specific magnetic field, while adipogenic differentiation remains unchanged.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Ye Zhu, Ren Li, Li-Ming Wen
Summary: The study found that XIST regulates the chondrogenic differentiation of SMSCs through the miR-27b-3p/ADAMTS-5 axis, offering new targets for TMJOA treatment.
Article
Cell & Tissue Engineering
Daniele Zuncheddu, Elena Della Bella, Dalila Petta, Cecilia Bartschi, Sonja Hackel, Moritz C. Deml, Martin J. Stoddart, Sibylle Grad, Valentina Basoli
Summary: This study investigates the effects of glucose and fructose on the chondrogenic differentiation of BMSCs. The results show that fructose may enhance chondrogenic differentiation, while decreasing glucose concentration may decrease differentiation yield. The study also reveals the expression and regulation of GLUT3 and GLUT5 in BMSCs during chondrogenesis. The findings provide valuable insights into the sugar metabolism of BMSCs during cartilage formation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Masato Kobayashi, Ryota Chijimatsu, David A. Hart, Shuichi Hamamoto, George Jacob, Fumiko Yano, Taku Saito, Kazunori Shimomura, Wataru Ando, Ung-il Chung, Sakae Tanaka, Hideki Yoshikawa, Norimasa Nakamura
Summary: This study investigated the effect of TD-198946 pretreatment on hSSCs and found that TD-198946 enhances the chondrogenic potential of hSSCs through the NOTCH3 signaling pathway. The activation of NOTCH3 by TD-198946 provides a novel insight into regulating the differentiation of hSSCs into chondrocytes.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Marcin Szustak, Edyta Gendaszewska-Darmach
Summary: Nanocellulose is a promising biocompatible biomaterial with good mechanical properties for use as a scaffold in cartilage tissue engineering. The limited regenerative capacity of cartilage due to its lack of vascularization and sparsely distributed chondrocytes poses challenges for cell infiltration, making nanocellulose scaffolds a potential solution. The scaffolds have been shown to induce stem cell differentiation into chondrocyte phenotypes, offering opportunities for cartilage tissue development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell & Tissue Engineering
Anastasiia D. Kurenkova, Lei Li, Anna P. Usanova, Xiaogang Feng, Baoyi Zhou, Andrei A. Nedorubov, Alexey Lychagin, Andrei S. Chagin
Summary: Articular cartilage has limited ability to self-repair, and clinical approaches to cartilage regeneration are necessary. The current method involves expansion of autologous chondrocytes followed by reimplantation, but the outcome is often unsatisfactory due to dedifferentiation. In recent years, articular stem/progenitor cells (artSPCs) have been discovered, which have the potential to repair damaged cartilage. This study found that modulation of the Notch pathway during expansion facilitated chondrogenic potential of artSPCs and chondrocytes, while activation of the pathway abrogated this potential.
Review
Immunology
Sicheng Zhang, Zhen Duan, Fang Liu, Qingjie Wu, Xiwei Sun, Hailong Ma
Summary: Rheumatoid arthritis (RA) is an autoimmune disease that affects joint health. Exosomes, which are secreted vesicles, contain various substances and play a role in cellular communication. Recent studies have shown that exosomes derived from different cells can influence the biological functions of immune cells and joint cells, but the exact mechanisms are still unclear. This comprehensive review provides a scientific foundation for utilizing exosomes as diagnostic targets and therapeutic approaches in the context of RA.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell & Tissue Engineering
Mohamed Elashry, Mebrie Kinde, Michele C. Klymiuk, Asmaa Eldaey, Sabine Wenisch, Stefan Arnhold
Summary: In this study, it was found that exposure to hypoxia enhanced the proliferation and motility of skeletal muscle-derived stem cells, promoted the differentiation of muscle and adipocytes, and improved the efficiency of myoblast differentiation.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Natalia Linkova, Vladimir Khavinson, Anastasiia Diatlova, Svetlana Myakisheva, Galina Ryzhak
Summary: Finding innovative ways to treat osteoarthritis (OA) is a pressing task in molecular medicine and biogerontology. Directed differentiation of mesenchymal stem cells (MSCs) into chondrocytes is considered a potential method for OA treatment. This review examines the main molecules involved in the chondrogenic differentiation of MSCs, with peptides derived from growth factors' structures and components of cartilage tissue showing promising results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Lingxiao Huang, Zhenni Xu, Xudan Lei, Yujun Huang, Siyu Tu, Lu Xu, Jieying Xia, Dengqun Liu
Summary: This study reveals the important role of inducible nitric oxide synthase (iNOS) in the intestinal crypts and its effects on intestinal stem cell (ISC) proliferation and differentiation. iNOS deficiency leads to morphological changes in the intestine and alters gluconeogenesis and the adaptive immune response. These findings highlight the potential of iNOS as a therapeutic target for intestinal disorders.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Wang Tang, Hongyi Zhang, Donghua Liu, Feng Jiao
Summary: The study demonstrated that ICA or BMSCs alone could repair rabbit knee cartilage damage, while the combined treatment of ICA and BMSCs showed better ability in repairing rabbit knee cartilage damage.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2022)
Article
Biology
Kathryn Futrega, Pamela G. Robey, Travis J. Klein, Ross W. Crawford, Michael R. Doran
Summary: The study challenges the long-standing belief that BMSC differentiation requires over 2 weeks of continuous TGF-beta 1 exposure, showing that differentiation can be triggered by just a single day of exposure through the use of a micro-pellet model.
COMMUNICATIONS BIOLOGY
(2021)