Article
Biochemistry & Molecular Biology
Yushi Abe, Daigo Ochiai, Masako Taguchi, Seiji Kanzaki, Satoru Ikenoue, Yoshifumi Kasuga, Mamoru Tanaka
Summary: Human amniotic fluid stem cells (hAFSCs) can form aggregates with host immune cells and alleviate inflammation. Additionally, hAFSCs can increase regulatory T cells in the acquired immune system. Therefore, hAFSCs play an important role in the treatment of inflammation-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Athanasia Dasargyri, Dayme Gonzalez Rodriguez, Hubert Rehrauer, Ernst Reichmann, Thomas Biedermann, Ueli Moehrlen
Summary: This study characterized the tissue origin and marker expression of cultured amniotic fluid cells at the single-cell level using single-cell RNA sequencing. It revealed the heterogeneity of amniotic fluid cells and identified cell types of neural origin in fetuses with spina bifida aperta. The findings provide valuable information for further research and clinical applications of amniotic fluid cells.
Article
Cell Biology
Francesca Casciaro, Silvia Zia, Mattia Forcato, Manuela Zavatti, Francesca Beretti, Emma Bertucci, Andrea Zattoni, Pierluigi Reschiglian, Francesco Alviano, Laura Bonsi, Matilde Yung Follo, Marco Demaria, Barbara Roda, Tullia Maraldi
Summary: This study utilized Celector® technology to classify and characterize hAFSCs, uncovering deep intra-population differences and validating their impact on stemness properties. The results suggest the importance of selecting cellular fractions with the highest potential for use in regenerative medicine.
Article
Cell Biology
Marcin Michalik, Patrycja Wieczorek, Piotr Czekaj
Summary: The effectiveness of differentiating hAECs into hepatic cells can be enhanced by culturing them with high concentrations of HGF or EGF. HGF is more effective in increasing the expression of liver-specific genes, while EGF stimulates the differentiation process more efficiently, resulting in more hepatocyte-like cells capable of synthesizing alpha-fetoprotein.
Article
Biotechnology & Applied Microbiology
Aiste Zentelyte, Deimante Zukauskaite, Ieva Jaceryte, Veronika V. Borutinskaite, Ruta Navakauskiene
Summary: In this study, short-term treatments of small molecules were used to enhance the properties and differentiation capability of human amniotic fluid stem cells (AFSC). The combinations of epigenetically active compounds and multifunctional molecules upregulated genes involved in pluripotency, leading to a more energetically active phenotype in AFSCs. The small molecule treatments also showed a beneficial effect on differentiation potential towards a neurogenic lineage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Cell Biology
Nengqing Liu, Yi Cheng, Ding Wang, Hongmei Guan, Diyu Chen, Juan Zeng, Dian Lu, Yuanshuai Li, Yinghong Yang, Qian Luo, Lifen Zhu, Bin Jiang, Xiaofang Sun, Bing Song
Summary: Amniotic fluid derived mesenchymal stem cells (AFMSCs) exhibit superior multipotency and immunomodulatory properties compared to MSCs derived from other somatic tissues. This study categorized AFMSCs into three distinct groups and found that a minority population (AFMSCs-X) displayed the highest multipotency and immunomodulatory abilities. Experimental results in a septic mice model showed that AFMSCs-X significantly improved survival rates and reduced bacterial colonization.
Article
Cardiac & Cardiovascular Systems
Jiajia Hu, Xuliang Chen, Ping Li, Xiaoxu Lu, Jianqin Yan, Huiling Tan, Chengliang Zhang
Summary: Research has shown that hAFMSCExos have proangiogenic effects on endothelial cells and can enhance angiogenesis in cardiac fibrosis. These exosomes may be a promising potential treatment strategy for cardiac fibrosis.
CARDIOVASCULAR DIAGNOSIS AND THERAPY
(2021)
Article
Multidisciplinary Sciences
Carolina C. Zuliani, Ingrid I. Damas, Kleber C. Andrade, Cecilia B. Westin, Angela M. Moraes, Ibsen Bellini Coimbra
Summary: The study reveals the potential of amniotic fluid stem cells (AFSCs) combined with scaffolds in reconstructing articular cartilage, showing successful differentiation into chondrocytes. The experiments demonstrated that AFSCs cultured in both a micromass system and directly in a scaffold can differentiate into chondrocytes effectively.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Giulia Gaggi, Andrea Di Credico, Simone Guarnieri, Maria Addolorata Mariggio, Angela Di Baldassarre, Barbara Ghinassi
Summary: Human amniotic fluids stem cells (hAFSCs) can differentiate into spinal motor neurons and form functional neuromuscular junctions, providing a promising approach for the study and treatment of neurodegenerative diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Yushi Abe, Daigo Ochiai, Seiji Kanzaki, Yu Sato, Toshimitsu Otani, Satoru Ikenoue, Yoshifumi Kasuga, Mamoru Tanaka
Summary: This study demonstrates that hAFSCs are effective in the treatment and prevention of inflammation-induced preterm birth. hAFSCs suppress inflammation and reduce preterm birth by switching macrophage polarity.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Review
Medicine, General & Internal
Han Luo, Zhen Wang, Fang Qi, Dali Wang
Summary: Complete wound regeneration is crucial for maintaining skin structure and physiological functions, and amniotic fluid stem cells (AFSCs) have the potential to play a significant role in wound generation and treatment. AFSCs are easily accessible and have advantages such as anti-inflammatory and immunomodulatory properties, which can promote full wound regeneration.
CHINESE MEDICAL JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Ambra Costa, Rodolfo Quarto, Sveva Bollini
Summary: Studies have shown that small extracellular vesicles (EVs) obtained from human amniotic fluid stem cells (hAFSC) and human amniotic fluid (hAF) have regenerative effects and can be used for tissue recovery and treatment of various diseases. However, the isolation, characterization, and quantification methods of EVs remain challenging for clinical translation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Obstetrics & Gynecology
Monique M. Martin, Michael Chan, Clarel Antoine, Liron Bar-El, Eran Bornstein, Bruce K. Young
Summary: This study investigated whether amniotic fluid-derived stem cells maintained their stem cell characteristics after processing by a licensed cell therapy center and simulated clinical application. The results showed that amniotic fluid stem cells processed in a clinical cell therapy center behaved similarly to those processed in a research laboratory, exhibiting viability, stability, growth, differentiation, and markers of stemness. The study also found that amniotic fluid cells maintained a stable viability at room temperature for a minimum of 3 hours when passed through a clinically approved delivery device. These findings suggest that human amniotic fluid processed in a clinical facility may have therapeutic potential if proven safe.
JOURNAL OF PERINATAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Francesca Casciaro, Francesca Beretti, Martina Gatti, Giuseppe Persico, Emma Bertucci, Marco Giorgio, Tullia Maraldi
Summary: Human amniotic fluid cells (hAFSCs) are a heterogeneous population with stem cell characteristics. This study compares the gene expression profiles of adherent amniotic fluid cells and their c-Kit(+) subpopulation through single-cell and bulk RNAseq analysis. Differences in cellular distribution, pathways related to stemness, and secretome composition were identified between the two populations. Selecting c-Kit positive fractions with higher potential could be important in regenerative medicine applications.
Article
Cell & Tissue Engineering
Valentina Orticelli, Andrea Papait, Elsa Vertua, Patrizia Bonassi Signoroni, Pietro Romele, Lorena Di Pietro, Marta Magatti, Luciana Teofili, Antonietta Rosa Silini, Ornella Parolini
Summary: This study investigated the potential of human term placenta-derived amniotic membrane MSCs (hAMSC) to support the ex vivo expansion of HSC and progenitor cells. The results suggest that hAMSCs are a valuable alternative to BM-MSC for enhancing the proliferation and maintaining stemness of CB-HSC, and can improve the colony forming unit ability and long-term culture initiating cells ability, addressing the need for high HSC content in CB units available for transplantation.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Po-Yen Lin, Er-Yuan Chuang, Yi-Hsuan Chiu, Hsin-Lung Chen, Kun-Ju Lin, Jyuhn-Huarng Juang, Ching-Hua Chiang, Fwu-Long Mi, Hsing-Wen Sung
JOURNAL OF CONTROLLED RELEASE
(2017)
Article
Chemistry, Multidisciplinary
Wei-Lin Wan, Yu-Jung Lin, Hsin-Lung Chen, Chieh-Cheng Huang, Po-Chien Shih, Yu-Ru Bow, Wei-Tso Chia, Hsing-Wen Sung
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Article
Chemistry, Multidisciplinary
Er-Yuan Chuang, Kun-Ju Lin, Tring-Yo Huang, Hsin-Lung Chen, Yang-Bao Miao, Po-Yen Lin, Chiung-Tong Chen, Jyuhn-Huarng Juang, Hsing-Wen Sung
Article
Chemistry, Multidisciplinary
Yu-Jung Lin, Chun-Chieh Chen, Nai-Wen Chi, Trang Nguyen, Hung-Yun Lu, Dang Nguyen, Po-Liang Lai, Hsing-Wen Sung
ADVANCED MATERIALS
(2018)
Article
Chemistry, Multidisciplinary
Wei-Lin Wan, Yu-Jung Lin, Po-Chien Shih, Yu-Ru Bow, Qinghua Cui, Yen Chang, Tso Chia, Hsing-Wen Sung
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2018)
Article
Cardiac & Cardiovascular Systems
Sheng He, Huifang Song, Jun Wu, Shu-Hong Li, Richard D. Weisel, Hsing-Wen Sung, Jianding Li, Ren-Ke Li
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2018)
Article
Medicine, Research & Experimental
Zhi Cui, Nathan C. Ni, Jun Wu, Guo-Qing Du, Sheng He, Terrence M. Yau, Richard D. Weisel, Hsing-Wen Sung, Ren-Ke Li
Article
Chemistry, Multidisciplinary
Po-Yen Lin, Ya-Ling Chiu, Jing-Huei Huang, Er-Yuan Chuang, Fwu-Long Mi, Kun-Ju Lin, Jyuhn-Huarng Juang, Hsing-Wen Sung, Kam W. Leong
Article
Engineering, Biomedical
Wei-Chih Lin, Wen-Yu Pan, Chen-Kao Liu, Wu-Xuan Huang, Hsiang-Lin Song, Kai-Sheng Chang, Meng-Ju Li, Hsing-Wen Sung
Review
Pharmacology & Pharmacy
Yu-Jung Lin, Fwu-Long Mi, Po-Yen Lin, Yang-Bao Miao, Tringyo Huang, Kuan-Hung Chen, Chiung-Tong Chen, Yen Chang, Hsing-Wen Sung
ADVANCED DRUG DELIVERY REVIEWS
(2019)
Article
Chemistry, Multidisciplinary
Po-Yen Lin, Kuan-Hung Chen, Yang-Bao Miao, Hsin-Lung Chen, Kun-Ju Lin, Chiung-Tong Chen, Chun-Nan Yeh, Yen Chang, Hsing-Wen Sung
ADVANCED FUNCTIONAL MATERIALS
(2019)
Article
Engineering, Biomedical
Wei-Chih Lin, Chieh-Cheng Huang, Shu-Jyuan Lin, Meng-Ju Li, Yen Chang, Yu-Jung Lin, Wei-Lin Wan, Po-Chien Shih, Hsing-Wen Sung
Review
Engineering, Biomedical
Yu-Jung Lin, Chieh-Cheng Huang, Wei-Lin Wan, Ching-Hua Chiang, Yen Chang, Hsing-Wen Sung
Article
Nanoscience & Nanotechnology
Kun-Ying Lu, Po-Yen Lin, Er-Yuan Chuang, Chwen-Ming Shih, Tsai-Mu Cheng, Tsung-Yao Lin, Hsing-Wen Sung, Fwu-Long Mi
ACS APPLIED MATERIALS & INTERFACES
(2017)
Article
Engineering, Biomedical
Chiranjeevi Korupalli, Chieh-Cheng Huang, Wei-Chih Lin, Wen-Yu Pan, Po-Yen Lin, Wei-Lin Wan, Meng-Ju Li, Yen Chang, Hsing-Wen Sung