Review
Cardiac & Cardiovascular Systems
Michael Nguyen-Truong, Peiman Hematti, Zhijie Wang
Summary: MSCs have been studied for nearly two decades as a therapy for myocardial restoration, with a focus on their paracrine function. Utilizing MSC-derived cell-free approaches for regenerative therapy comes with advantages and disadvantages, and involves mechanisms such as angiogenesis and immunomodulation. Researchers are working towards enhancing these properties to improve therapeutic outcomes, while facing remaining challenges in the field.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Miquel Antich-Rossello, Maria Antonia Forteza-Genestra, Marta Monjo, Joana M. Ramis
Summary: Platelet-derived EVs (pEVs) show great potential as a new therapeutic approach in wound healing and regenerative medicine. Studies have demonstrated that pEVs not only participate in healing responses but also induce cellular differentiation, promoting musculoskeletal or neural regeneration. However, there is heterogeneity in the obtention and characterization of pEVs, requiring further research to enhance their translation into feasible therapeutic applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Chemistry, Multidisciplinary
Han Young Kim, Seunglee Kwon, Wooram Um, Sol Shin, Chan Ho Kim, Jae Hyung Park, Byung-Soo Kim
Summary: Extracellular vesicles (EVs), with their unique biological characteristics and clinical potential, have shown promise in the field of regenerative medicine. As important mediators of intercellular communication, naturally secreted EVs have the ability to promote tissue regeneration. However, challenges related to clinical translation have prompted further functionalization. In recent years, various engineering approaches and the development of artificial EVs have been used to enhance the therapeutic outcomes of EVs.
Review
Biochemistry & Molecular Biology
Enzo Manchon, Nell Hirt, Jean-David Bouaziz, Nabila Jabrane-Ferrat, Reem Al-Daccak
Summary: Endosome-derived small extracellular vesicles (EVs), also known as exosomes, play a critical role in intercellular communication and have therapeutic potential in regenerative medicine. Stem cell-derived EVs are effective in repair and regeneration, with the human placenta offering a promising allogeneic stem cell source. Advancements in cell-free therapeutics provide new hope and perspectives for safe and effective treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Charles M. Bowen, Frederick S. Ditmars, Ashim Gupta, Jo-Anna Reems, William Samuel Fagg
Summary: Amniotic fluid, specifically its cell-free form, has regenerative properties and can stimulate immunomodulatory and pro-growth characteristics in target cells or tissues. It has been shown to effectively treat cutaneous wounds and orthopedic degenerative conditions.
Article
Medicine, Research & Experimental
Halimeh Mobarak, Mohammad Heidarpour, Reza Rahbarghazi, Mohammad Nouri, Mandi Mahdipour
Summary: This study aimed to investigate the therapeutic effects of AF-Exos on sperm production capacity in a rat model of azoospermia, showing significant improvement in spermatogenesis and sperm parameters post-injection. The increase of OCT-3/4(+) cells, expression of DAZL and VASA, and restoration of testicular function were observed in NOA rats treated with AF-Exos.
Article
Biotechnology & Applied Microbiology
Ambra Costa, Carolina Balbi, Patrizia Garbati, Maria Elisabetta Federica Palama, Daniele Reverberi, Antonella De Palma, Rossana Rossi, Dario Paladini, Domenico Coviello, Pierangela De Biasio, Davide Ceresa, Paolo Malatesta, Pierluigi Mauri, Rodolfo Quarto, Chiara Gentili, Lucio Barile, Sveva Bollini
Summary: Cardiomyocyte renewal is a clinical need for cardiac regeneration, and stem cell paracrine therapy has been shown to provide cardiac protection and enhance repair. In this study, it was found that extracellular vesicles released by fetal amniotic fluid-derived stem cells could promote cardiomyocyte renewal, possibly through the mediation of factors such as Agrin.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Engineering, Biomedical
Tyler J. DiStefano, Keti Vaso, George Danias, Henry N. Chionuma, Jennifer R. Weiser, James C. Iatridis
Summary: Research has shown that stem cell-derived EVs can slow down the progression of IVDD at the molecular, cellular, and organ levels. The pathophysiological and differentiation state of the EV parent cell affects downstream treatment responses and therapeutic outcomes. These findings support the use of EVs as a promising cell-free strategy for treating IVDD and enhancing endogenous repair.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Cell Biology
Nikolaos Panagiotou, Dagmara McGuinness, Armand M. G. Jaminon, Barend Mees, Colin Selman, Leon Schurgers, Paul G. Shiels
Summary: Extracellular vesicles (EVs) secreted by cells play a role in repairing cellular and tissue damage. Microvesicles (MVs) from stem cell regulatory cells have been found to enhance the repair of human dermal fibroblasts (HDFs) and mesenchymal stem cells (MSCs) under stress, but this effect is age and stress specific. These findings provide insights into the roles of EVs in tissue repair and ageing.
Review
Biochemistry & Molecular Biology
Yasunari Matsuzaka, Ryu Yashiro
Summary: Extracellular vesicles (EVs) are lipid bilayer membrane particles that play critical roles in intracellular communication. Mesenchymal stem cells (MSCs) induce tissue regeneration by direct recruitment into injured tissues or secreting factors. Recent studies have shown that MSC-derived EVs have regenerative effects against various diseases and can serve as drug delivery systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Eduardo Anitua, Maria Troya, Juan Manuel Falcon-Perez, Silvia Lopez-Sarrio, Esperanza Gonzalez, Mohammad H. Alkhraisat
Summary: The use of platelet-rich plasma (PRP) has attracted increasing interest due to its rich content of growth factors, cytokines, chemokines, and other biomolecules. Recent research has focused on platelets' released extracellular vesicles (EVs) and their role in intercellular communication. This review aims to compile the current evidence on PRP-derived EVs and evaluate their advantages and limitations for clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Luis A. Costa, Noemi Eiro, Maria Fraile, Luis O. Gonzalez, Jorge Saa, Pablo Garcia-Portabella, Belen Vega, Jose Schneider, Francisco J. Vizoso
Summary: Studies have shown that mesenchymal stem cells (MSC) have therapeutic potential in various organs and tissues. However, their functional heterogeneity poses a challenge in transferring their capabilities to clinical applications. Proper selection of MSC based on their functional potential and improving their features in the artificial niche are essential for personalized and precise medicine.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
Maryam Adelipour, David M. Lubman, Jeongkwon Kim
Summary: This article provides a comprehensive overview of regenerative medicine, focusing on the use of mesenchymal stem cells (MSCs) and their exosomes as potential therapies for damaged cells, tissues, or organs. The article highlights the unique advantages of MSCs and their secreted exosomes, including immunomodulatory effects, lack of immunogenicity, and recruitment to damaged areas. It also discusses challenges and proposed solutions for improving MSC or exosome therapy. Literature search was conducted using Google Scholar and PubMed databases.
EXPERT OPINION ON BIOLOGICAL THERAPY
(2023)
Article
Neurosciences
Masoumeh Pourhadi, Hakimeh Zali, Rasoul Ghasemi, Saeed Vafaei-Nezhad
Summary: Mesenchymal stem cells and their derived extracellular vesicles, especially those derived from the oral cavity, have shown promising potential in improving neurodegenerative diseases and promoting neuroregeneration.
MOLECULAR NEUROBIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Marta Bryk, Elzbieta Karnas, Jakub Mlost, Ewa Zuba-Surma, Katarzyna Starowicz
Summary: MSCs, as multipotent progenitor cells derived from mesoderm, have been extensively studied for their regenerative properties, including the release of EVs which play a role in their functions. They regulate various processes in target cells and show potential applications in pain treatment and other diseases.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Editorial Material
Cardiac & Cardiovascular Systems
Sveva Bollini, Tomasz J. Guzik
Summary: The commentary summarizes the crucial role of the immune system in driving the aging and senescence of solid organs, offering a new perspective for understanding age-related pathologies.
CARDIOVASCULAR RESEARCH
(2022)
Article
Chemistry, Physical
Paolo Canepa, Giuseppe Firpo, Elena Gatta, Roberto Spotorno, Paolo Giannoni, Rodolfo Quarto, Maurizio Canepa, Ornella Cavalleri
Summary: We propose a two-step surface modification process to tailor the micro and nano morphology of niobium oxide layers. Firstly, niobium undergoes anodization in a solution containing calcium and phosphorus, followed by acid etching. The effects of anodization time and applied potential on surface morphology are investigated, and subsequently, the surface nano morphology is tuned by acid etching. In vitro tests show that the etching-induced nanostructure effectively promotes cell adhesion and spreading onto the niobium oxide surface.
Review
Oncology
Barbara Cardinali, Roberta Tasso, Patrizia Piccioli, Maria Chiara Ciferri, Rodolfo Quarto, Lucia Del Mastro
Summary: This review discusses the potential application of circulating microRNAs (miRNAs) as diagnostic and monitoring biomarkers in breast cancer. It highlights the advantages and challenges that need to be addressed. Although further studies are needed to support their clinical utility, miRNAs could represent novel tools for breast cancer management.
Review
Cardiac & Cardiovascular Systems
Paul C. Evans, Sean M. Davidson, Johann Wojta, Magnus Baeck, Sveva Bollini, Mairi Brittan, Alberico L. Catapano, Bill Chaudhry, Matthijs Cluitmans, Massimiliano Gnecchi, Tomasz J. Guzik, Imo Hoefer, Rosalinda Madonna, Joao P. Monteiro, Henning Morawietz, Elena Osto, Teresa Padro, Judith C. Sluimer, Carlo Gabriele Tocchetti, Kim Van der Heiden, Gemma Vilahur, Johannes Waltenberger, Christian Weber
Summary: This article reviews the highlights of cardiovascular basic science published in 2021 and early 2022. It covers topics including non-coding RNAs, single-cell 'omics, extracellular vesicles, cardiovascular sensing of mechanical force, atherosclerosis, cardiac arrhythmia, inducible pluripotent stem cell technology, and the association between COVID-19 and the cardiovascular system.
CARDIOVASCULAR RESEARCH
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
A. Costa, C. Balbi, P. Garbati, A. De Palma, D. Ceresa, S. Santamaria, K. Cortese, D. Coviello, P. De Biasio, D. Paladini, L. Barile, P. Malatesta, P. Mauri, S. Bollini
CARDIOVASCULAR RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Barbara Cardinali, Giuseppa De Luca, Roberta Tasso, Simona Coco, Anna Garuti, Giulia Buzzatti, Andrea Sciutto, Luca Arecco, Federico Villa, Franca Carli, Daniele Reverberi, Rodolfo Quarto, Mariella Dono, Lucia Del Mastro
Summary: This study investigates PIK3CA mutations in different circulating biomaterials and demonstrates the potential of these components to provide information on molecular targetable profiling. The consistent mutational profiles between extracellular vesicles and tumor cells suggest their association. This study also highlights the translational value of circulome in understanding the tumor heterogeneity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Ambra Costa, Carolina Balbi, Patrizia Garbati, Maria Elisabetta Federica Palama, Daniele Reverberi, Antonella De Palma, Rossana Rossi, Dario Paladini, Domenico Coviello, Pierangela De Biasio, Davide Ceresa, Paolo Malatesta, Pierluigi Mauri, Rodolfo Quarto, Chiara Gentili, Lucio Barile, Sveva Bollini
Summary: Cardiomyocyte renewal is a clinical need for cardiac regeneration, and stem cell paracrine therapy has been shown to provide cardiac protection and enhance repair. In this study, it was found that extracellular vesicles released by fetal amniotic fluid-derived stem cells could promote cardiomyocyte renewal, possibly through the mediation of factors such as Agrin.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Michela Pozzobon, Stefania D'Agostino, Maria G. G. Roubelakis, Anna Cargnoni, Roberto Gramignoli, Susanne Wolbank, Florelle Gindraux, Sveva Bollini, Halima Kerdjoudj, Mathilde Fenelon, Roberta Di Pietro, Mariangela Basile, Veronika Borutinskaite, Roberta Piva, Andreina Schoeberlein, Guenther Eissner, Bernd Giebel, Peter Ponsaerts
Summary: Perinatal tissues contain various types of stem cells that have the potential for regenerative therapies. This review summarizes the therapeutic approaches using perinatal derivatives (PnD) products and discusses the importance of validating their therapeutic efficacy and the concept of PnD application in tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Cansu Gorgun, Chiara Africano, Maria Chiara Ciferri, Nadia Bertola, Daniele Reverberi, Rodolfo Quarto, Silvia Ravera, Roberta Tasso
Summary: This study demonstrates that EVs derived from MSCs preconditioned with hypoxia inflammatory cytokines can attenuate inflammaging and alleviate age-related inflammation and mitochondrial dysfunction.
Article
Dermatology
Giulia Gasparini, Roberta Tasso, Maria Elisabetta Federica Palama, Maria Chiara Ciferri, Chiara Gentili, Giovanni Di Zenzo, Alessia Provini, Adele Salemme, Rodolfo Quarto, Aurora Parodi, Emanuele Cozzani
Summary: This study aimed to investigate the presence of BP targeted autoantigens in blister fluid-derived extracellular vesicles (EVs). EVs were isolated from blister fluid of BP patients and suction blister fluid of healthy donors, and EV characterization was performed. Western blot analysis revealed the presence of BP180 in EVs derived from blister fluid of 3 out of 6 BP patients, while no BP180 was detected in EVs from healthy donors. This discovery provides insights into the pathogenesis of BP.
ARCHIVES OF DERMATOLOGICAL RESEARCH
(2023)
Meeting Abstract
Cell & Tissue Engineering
Federico Villa, Ambra Costa, Silvia Bruno, Mingchuan Li, Michele Russo, James Cimino, Clarissa Ruggeri, Rodolfo Quarto, Pietro Ameri, Alessandra Ghigo, Silvia Ravera, Bollini Sveva, Roberta Tasso
TISSUE ENGINEERING PART A
(2022)