Article
Multidisciplinary Sciences
Ranjith P. Anand, Jacob V. Layer, David Heja, Takayuki Hirose, Grace Lassiter, Daniel J. Firl, Violette B. Paragas, Adam Akkad, Sagar Chhangawala, Robert B. Colvin, Russell J. Ernst, Nicholas Esch, Kristen Getchell, Alexandra K. Griffin, Xiaoyun Guo, Katherine C. Hall, Paula Hamilton, Lokesh A. Kalekar, Yinan Kan, Ahmad Karadagi, Feng Li, Susan C. Low, Rudy Matheson, Claudia Nehring, Ryo Otsuka, Matthew Pandelakis, Robert A. Policastro, Rebecca Pols, Luis Queiroz, Ivy A. Rosales, William T. Serkin, Kathryn Stiede, Toshihide Tomosugi, Yongqiang Xue, Gabriel E. Zentner, David Angeles-Albores, J. Chris Chao, Juliet N. Crabtree, Sierra Harken, Nicole Hinkle, Tania Lemos, Mailin Li, Lorena Pantano, Denise Stevens, Omar D. Subedar, Xiaoqing Tan, Shiyi Yin, Imran J. Anwar, David Aufhauser, Saverio Capuano, Dixon B. Kaufman, Stuart J. Knechtle, Jean Kwun, Dhanansayan Shanmuganayagam, James F. Markmann, George M. Church, Mike Curtis, Tatsuo Kawai, Michele E. Youd, Wenning Qin
Summary: This study explores the design, creation, and long-term function of kidney grafts from a genetically engineered porcine donor transplanted into a cynomolgus monkey model. The study shows successful preclinical studies of renal xenotransplantation in nonhuman primates and brings us closer to clinical trials of genetically engineered porcine renal grafts.
Article
Agriculture, Dairy & Animal Science
Koki Takebayashi, Manita Wittayarat, Qingyi Lin, Maki Hirata, Naoaki Yoshimura, Nanaka Torigoe, Megumi Nagahara, Lanh Thi Kim Do, Fuminori Tanihara, Takeshige Otoi
Summary: CRISPR/Cas9 technology has greatly influenced the efficiency and effectiveness of genetic modifications. The study found that electroporation alone significantly increased the frequency of biallelic mutations, while lipofection alone required the removal of the zona pellucida to enable CRISPR/Cas9-based genome editing. Additionally, additional lipofectamine treatment did not improve the mutation rates.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Cell & Tissue Engineering
Preeti Vats, Ramakant Kaushik, Nidhi Rawat, Ankur Sharma, Tushar Sharma, Diksha Dua, Manoj Kumar Singh, Prabhat Palta, Suresh Kumar Singla, Radhey Sham Manik, Manmohan Singh Chauhan
Summary: Utilizing TALEN-mediated transgene integration at the safe harbor locus Rosa 26 in the goat genome resulted in stable gene expression and successful generation of transgenic cloned embryos. Transfection of GFFs with TALEN yielded correctly targeted cell clones with monoallelic gene insertions.
CELLULAR REPROGRAMMING
(2021)
Article
Cell Biology
Pantu Kumar Roy, Ahmad Yar Qamar, Bereket Molla Tanga, Xun Fang, Ghangyong Kim, Seonggyu Bang, Jongki Cho
Summary: The study investigated the effect of milrinone supplementation during in vitro maturation on porcine oocytes and cloned embryo development. Results showed that milrinone improved maturation and blastocyst development rates in less competent oocytes, potentially due to coordinated cytoplasmic and nuclear maturation. Milrinone supplementation also led to higher expression levels of reprogramming-related genes in oocytes, indicating its usefulness for proper nuclear reprogramming in cloned embryo production.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Veterinary Sciences
Zhao Namula, Maki Hirata, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Naoaki Yoshimura, Fuminori Tanihara, Chommanart Thongkittidilok, Takeshige Otoi
Summary: The study showed that weakening the ZP does not affect the developmental competence, mutation rate, or mutation efficiency of electroporated zygotes, while ZP removal has a detrimental effect on embryonic development but may increase the mutation rate.
VETERINARY MEDICINE AND SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Celia Pineiro-Silva, Sergio Navarro-Serna, Ramses Belda-Perez, Joaquin Gadea
Summary: Lipofection treatment, which involves encapsulating the CRISPR/Cas9 system into vesicles and introducing them into cells, can be a simple and inexpensive method for producing genetically modified pigs. It has similar effects on embryo development and mutation rates as the electroporation technique when used in zona-pellucida-intact porcine oocytes. However, increasing the concentration of liposomes-CRISPR/Cas9 system complexes in the media has a detrimental effect on embryo development parameters, possibly indicating a toxic effect.
Review
Cell & Tissue Engineering
Sarah Madani, Zoltan Machaty, Gabor Vajta
Summary: Increasing data suggests that the presence of the zona pellucida is not necessary for mammalian embryo production. Zona-free embryo culture systems have shown feasibility and potential in improving efficiency in transgenic research and human-assisted reproduction.
CELLULAR REPROGRAMMING
(2022)
Article
Cell Biology
Werner Giehl Glanzner, Mariana Priotto de Macedo, Karina Gutierrez, Vilceu Bordignon
Summary: Animal cloning has been successful in over 20 mammalian species using SCNT, with pigs being the leading species. The importance of pig cloning lies in its relevance to food production and biomedical applications. Despite recognized progress, the overall efficiency of SCNT in pigs remains low. New strategies for chromatin and epigenetic reprogramming may revolutionize pig cloning.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Francesca Barone, Juan Amaral, Irina Bunea, Mitra Farnoodian, Rohan Gupta, Rishabh Gupta, Dara Baker, M. Joseph Phillips, Richard J. Blanch, Arvydas Maminishkis, David M. Gamm, Kapil Bharti
Summary: Over 30 million people worldwide are suffering from untreatable vision loss and blindness caused by degeneration of photoreceptor, retinal pigment epithelium, and choriocapillaris. Lack of large-animal models for testing safety and efficacy of clinical doses covering the human macula has hindered the development of effective cell therapies. A versatile pig model that mimics different types and stages of retinal degeneration has been developed. This model is optimal for testing cell and gene therapies for outer retinal diseases including AMD and can facilitate faster translation to patients with clinically relevant imaging outcomes.
Article
Biology
Peng Chen, Shangping Wang, Zhenzhen Chen, Pengling Ren, R. Glenn Hepfer, Elizabeth D. Greene, Lia H. Campbell, Kristi L. Helke, Xingju Nie, Jens H. Jensen, Cherice Hill, Yongren Wu, Kelvin G. M. Brockbank, Hai Yao
Summary: Successful nanowarming and ice-free cryopreservation of large articular cartilage samples using nanoparticles is achieved, with significantly enhanced post-warming performance. The developed method shows a heating rate of over one order of magnitude higher than conventional warming methods, and a depth-dependent preservation manner is observed and confirmed. The delivery of nanoparticles to the OCA bone side is a potential direction for further optimization of the method. This study pioneers the application of nanowarming and ice-free cryopreservation for large articular cartilage and provides valuable insights for future technique development, paving the way for clinical applications of cryopreserved cartilage.
COMMUNICATIONS BIOLOGY
(2023)
Article
Genetics & Heredity
Kate Watson, Irving Korman, Yanhe Liu, Deirdre Zander-Fox
Summary: Live birth can be achieved in patients with zona production deficiency. Adjustments in ovarian stimulation and subsequent embryo culture strategies may have contributed to the success of the third cycle.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Weihong Fan, Misato Homma, Renliang Xu, Hiroki Kunii, Hanako Bai, Manabu Kawahara, Toshikazu Kawaguchi, Masashi Takahashi
Summary: The zona pellucida (ZP) is crucial for fertilization and embryonic development. Removing the ZP (ZPR) is necessary for various reproductive techniques, but traditional methods can have negative effects on development. This study shows that a two-step ZPR method combined with a customized well-of-well system can improve blastocyst rate and reduce toxic effects on zona free (ZF) embryo development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Hideki Kanda, Daigo Ando, Rintaro Hoshino, Tetsuya Yamamoto, Wahyudiono, Shogo Suzuki, Satoshi Shinohara, Motonobu Goto
Summary: The decellularization of porcine aortic tissue using subcritical DME was successfully conducted without the need for surfactants. Experimental results showed that this method effectively removed cell nuclei and reduced the remaining DNA content below the standard value.
Article
Agriculture, Dairy & Animal Science
Keisuke Sasaki, Saaya Takaoka, Yayoi Obata
Summary: By knocking amiRNA sequences against EGFP into the intronic sites of the Zp3 gene, an oocyte-specific knockdown system was successfully established, demonstrating size-dependent reduction of EGFP fluorescence in oocytes.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2021)
Article
Multidisciplinary Sciences
Sirilug Magerd, Thanyaporn Senarai, Orawan Thongsum, Chidchanok Chawiwithaya, Chihiro Sato, Ken Kitajima, Wattana Weerachatyanukul, Somluk Asuvapongpatana, Piyaporn Surinlert
Summary: This study characterized the presence of O-beta(1,4)-GlcNAc polymers on shrimp thrombospondin and found that they play a role in the structural architecture of the extracellular matrix.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
R. Li, Y. Liu, H. S. Pedersen, H. Callesen
REPRODUCTION IN DOMESTIC ANIMALS
(2018)
Article
Genetics & Heredity
Eduardo M. Razza, Hanne S. Pedersen, Lotte Stroebech, Patricia K. Fontes, Haja N. Kadarmideen, Henrik Callesen, Maria Pihl, Marcelo F. G. Nogueira, Poul Hyttel
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2019)
Article
Biochemical Research Methods
Mihai V. Curtasu, Knud Erik B. Knudsen, Henrik Callesen, Stig Purup, Jan Stagsted, Mette S. Hedemann
JOURNAL OF PROTEOME RESEARCH
(2019)
Article
Toxicology
Hanne Skovsgaard Pedersen, Ying Liu, Leslie Foldager, Henrik Callesen, Knud Larsen, Martin Tang Sorensen
TOXICOLOGY IN VITRO
(2019)
Article
Cell Biology
Beatriz Dorado, Gro Grunnet Ploen, Ana Barettino, Alvaro Macias, Pilar Gonzalo, Maria Jesus Andres-Manzano, Cristina Gonzalez-Gomez, Carlos Galan-Arriola, Jose Manuel Alfonso, Manuel Lobo, Gonzalo J. Lopez-Martin, Antonio Molina, Raul Sanchez-Sanchez, Joaquin Gadea, Javier Sanchez-Gonzalez, Ying Liu, Henrik Callesen, David Filgueiras-Rama, Borja Ibanez, Charlotte Brandt Sorensen, Vicente Andres
Article
Reproductive Biology
Ying Liu, Hanne Skovsgaard Pedersen, Knud Larsen, Kaja Kjaer Kristensen, Leslie Foldager, Henrik Callesen, Martin Tang Sorensen
REPRODUCTIVE TOXICOLOGY
(2019)
Review
Biodiversity Conservation
Julie Strand, Helena Thomsen, Julie B. Jensen, Cecilie Marcussen, Trine B. Nicolajsen, Mathias B. Skriver, Isabella M. Sogaard, Tariq Ezaz, Stig Purup, Henrik Callesen, Cino Pertoldi
CONSERVATION GENETICS RESOURCES
(2020)
Article
Cell Biology
Gianluca Mazzoni, Hanne S. Pedersen, Maria B. Rabaglino, Poul Hyttel, Henrik Callesen, Haja N. Kadarmideen
PHYSIOLOGICAL GENOMICS
(2020)
Article
Reproductive Biology
Hanne Skovsgaard Pedersen, Gianluca Mazzoni, Maria Pihl, Haja N. Kadarmideen, Poul Hyttel, Henrik Callesen
REPRODUCTIVE BIOLOGY
(2020)
Article
Agriculture, Dairy & Animal Science
Julie Strand, Henrik Callesen, Cino Pertoldi, Stig Purup
Summary: This study successfully established a cell line from a threatened amphibian species, highlighting the importance of using fresh tissue for cell line establishment, as well as confirming the effects of different media and contamination on cell growth.
Article
Biochemistry & Molecular Biology
Knud Larsen, Henrik Callesen
Summary: The expression levels of CREB1 mRNA show differential patterns in different brain tissues during pig embryo development, with a significant decrease in the brain stem and basal ganglia, and a significant increase in the lungs. However, there are no significant changes in the expression levels of NFATC2 mRNA in pig brain tissue during embryo development.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Cell Biology
Olav M. Andersen, Nikolaj Bogh, Anne M. Landau, Gro G. Ploen, Anne Mette G. Jensen, Giulia Monti, Benedicte P. Ulhoi, Jens R. Nyengaard, Kirsten R. Jacobsen, Margarita M. Jorgensen, Ida E. Holm, Marianne L. Kristensen, Aage Kristian O. Alstrup, Esben S. S. Hansen, Charlotte E. Teunissen, Laura Breidenbach, Mathias Droescher, Ying Liu, Hanne S. Pedersen, Henrik Callesen, Yonglun Luo, Lars Bolund, David J. Brooks, Christoffer Laustsen, Scott A. Small, Lars F. Mikkelsen, Charlotte B. Sorensen
Summary: This study developed a model of SORL1 haploinsufficiency using gene editing in minipigs, showing that this model can mimic the preclinical features of AD and providing functional support for the theory that SORL1 haploinsufficiency leads to AD.
CELL REPORTS MEDICINE
(2022)
Article
Zoology
Julie Strand, Henrik Callesen, Cino Pertoldi, Stig Purup
Summary: This study successfully established cell lines from the Asian Common Toad and found that tongue tissue had the highest success rate and more stable growth pattern. Cell growth patterns varied among individuals but were consistent within each individual. These findings can be used to improve the preservation and safeguarding of amphibian genetic material.
AMPHIBIAN & REPTILE CONSERVATION
(2022)
Article
Oncology
Martin Fogtmann Berthelsen, Maria Riedel, Huiqiang Cai, Soren H. Skaarup, Aage K. O. Alstrup, Frederik Dagnaes-Hansen, Yonglun Luo, Uffe B. Jensen, Henrik Hager, Ying Liu, Henrik Callesen, Mikkel H. Vendelbo, Jannik E. Jakobsen, Martin Kristian Thomsen
Summary: Research in large animal models has been simplified by the discovery of the CRISPR/Cas system, allowing for easier introduction of new gene alterations. A Cas9 minipig was cloned to generate a porcine model for pre-clinical research, with successful backcrossing and in vitro gene alterations confirmed. The development of a transgenic Cas9 minipig enables the creation of complex pre-clinical models for cancer research, including multiple gene alterations and in vivo activation of Cas9 expression.
Article
Agriculture, Dairy & Animal Science
Poul Hyttel, Lais Vicari de Figueiredo Pessoa, Jan Bojsen-Moller Secher, Katarina Stoklund Dittlau, Kristine Freude, Vanessa J. Hall, Trudee Fair, Remmy John Assey, Jozef Laurincik, Henrik Callesen, Torben Greve, Lotte Bjorg Stroebech
ANIMAL REPRODUCTION
(2019)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.