Article
Physiology
Shaharior Hossen, Soo Cheol Kim, Yusin Cho, Kang Hee Kho
Summary: The aim of this study was to improve the sperm cryopreservation protocol of marbled flounder by evaluating the vitality of 7-day and 1-year cryopreserved sperm. It was found that a cryopreservation protocol using sucrose solution combined with 15% dimethyl sulfoxide or 12% glycerol showed similar results to fresh sperm, indicating potential for hatchery and germplasm bank purposes. Differences were observed in plasma membrane integrity, mitochondrial membrane potential, and DNA integrity between fresh and cryopreserved sperm, highlighting the importance of selecting the appropriate cryoprotectant and extender for successful sperm cryopreservation.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Andrology
Odai A. M. Alqawasmeh, Mingpeng Zhao, Carol P. S. Chan, Maran B. W. Leung, Ki C. Chow, Nikunj Agarwal, Jennifer S. M. Mak, Chi C. Wang, Chi P. Pang, Tin C. Li, Wai K. Chu, David Y. L. Chan
Summary: Adding green tea extract (GTE) to sperm freezing media has a significant protective effect on human sperm motility and DNA integrity against oxidative stress. The supplementation of 1.0 ng ml(-1) GTE can increase sperm motility and DNA integrity, even in patients with high sperm DNA damage.
ASIAN JOURNAL OF ANDROLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Elba Rodriguez-Pena, Diego Suarez, Graciela Estevez-Perez, Patricia Verisimo, Noelia Barreira, Luis Fernandez, Ana Gonzalez-Tizon, Andres Martinez-Lage
Summary: Female spider crabs can store sperm for several years, but the viability and DNA integrity of the stored sperm decline sharply during the first few months.
Article
Fisheries
Sylwia Judycka, Mariola A. Dietrich, Daniel Zarski, Halina Karol, Katarzyna Palinska-Zarska, Maciej Blazejewski, Andrzej Ciereszko
Summary: This study aimed to investigate the effects of final glucose and sperm concentrations, different activation solutions for sperm motility, and dilution of semen on the cryopreservation success of cultured pikeperch semen. Results showed that specific concentrations and dilution methods had significant impacts on sperm motility and successful cryopreservation. Future efforts should focus on controlling spontaneous motility to further improve cryopreservation techniques for pikeperch semen.
Article
Multidisciplinary Sciences
Mirko Marino, Letizia Gigliotti, Peter Moller, Patrizia Riso, Marisa Porrini, Cristian Del Bo
Summary: The comet assay is used to assess DNA damage in tissues and biological samples. This study found that storage time had varying effects on DNA damage levels in PBMCs and WB samples, with significant increases observed in WB samples stored for over 6 months. WB samples showed higher levels of DNA damage compared to PBMCs.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Alexandre Erraud, Marc Bonnard, Valerie Cornet, Imen Ben Ammar, Sascha Antipine, Quentin Peignot, Jerome Lambert, Syaghalirwa N. M. Mandiki, Patrick Kestemont
Summary: By investigating the impact of different biological factors on sperm quality and reproductive success, it was found that sperm quality of early parr from captivity was poorer when subjected to cryopreservation, resulting in reduced reproductive success. Therefore, it is recommended that only the semen of anadromous males should be cryopreserved.
Article
Agriculture, Dairy & Animal Science
Shaimaa K. Hamad, Ahmed M. Elomda, Yanyan Sun, Yunlei Li, Yunhe Zong, Jilan Chen, Ahmed O. Abbas, Farid K. R. Stino, Ali Nazmi, Gamal M. K. Mehaisen
Summary: This study aims to evaluate the impact of different concentrations (3%, 6%, or 9%) of dimethylacetamide (DMA) as a cryoprotectant on post-thawed sperm quality, motility, antioxidant-biomarkers, and the expression of anti-freeze related genes. The results showed that 3% DMA group maintained higher post-thawed sperm quality compared to the other tested groups, with improvements in motility, viability, and antioxidant enzyme activity. Additionally, certain anti-freeze related genes were upregulated in the 3% DMA group.
Article
Reproductive Biology
Andre Furugen Cesar de Andrade, Kayode Balogun, Zoltan Machaty, Robert Victor Knox
Summary: This study aimed to evaluate the effect of supplementing a commercial freezing media with BHT, GSH, or their combination on in-vitro measures of boar sperm after cryopreservation. The results showed that adding GSH improved motility but decreased in vitro fertilization rate. Conversely, adding BHT enhanced the in vitro fertilizing ability of the frozen-thawed sperm cells.
Article
Reproductive Biology
Naomi Nakagata, Satohiro Nakao, Nobuyuki Mikoda, Katsuma Yamaga, Toru Takeo
Summary: This study used an inverted microscope to observe the process of fertilization and subsequent development of frozen-thawed rat sperm in vitro. It was found that the sperm attached to the zona pellucida 2-3 hours after thawing, penetrated into the oocytes after 3-5 hours, and formed pronuclei in the cytoplasm 5-9 hours after insemination. The frozen-thawed sperm from SD, Wistar, LE, and F344 strains showed higher fertility rates, while the sperm from the BN strain exhibited lower motility and fertility rates.
Article
Veterinary Sciences
Martina Colombo, Maria Giorgia Morselli, Jennifer Zahmel, Gaia Cecilia Luvoni
Summary: The aim of this study was to evaluate the morphofunctional integrity of cat epididymal spermatozoa ultra-rapid frozen in different extenders and to test whether spermatozoa preserved by the best combination were able to fertilize oocytes and produce embryos in vitro by intracytoplasmic sperm injection (ICSI). The results showed that cat epididymal spermatozoa better maintained their morphofunctional features after ultra-rapid freezing with a specific extender and could successfully produce embryos in vitro after ICSI.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
J. Beirao, S. Flengstad, I Babiak
Summary: A protocol for spotted wolffish sperm cryopreservation in 5-mL cryovials was developed to scale up the process, showing similar quality of cryopreserved sperm compared to traditional 0.5-mL straws under optimal freezing and thawing conditions.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Fisheries
Anatolii Sotnikov, Marek Rodina, Taisiya Stechkina, Cristiane Fernanda Benevente, David Gela, Sergii Boryshpolets, Vitaliy Kholodnyy, Otomar Linhart, Borys Dzyuba
Summary: To prevent mechanical compression of sperm during cryopreservation, a high dilution ratio is used. However, this results in increased sample volume and decreased post-thaw sperm motility. The study found that a lower dilution rate can be effective for carp sperm cryobanking, reducing cryo-storage space and required sperm volume without significantly affecting fertilization outcome.
Article
Fisheries
J. Nynca, S. Judycka, E. Liszewska, S. Dobosz, M. Krzys, A. Ciereszko
Summary: Freezing-thawing cycles have been found to preserve sperm motility in different fish species, but with a decrease in motility. Fertilizing ability of rainbow trout sperm did not differ after one freeze-thaw cycle, but significant decreases were recorded after two cycles. Double cryopreservation of fish semen shows potential benefits for preserving endangered fish species and valuable breeding aquaculture individuals.
Article
Biochemistry & Molecular Biology
Rosetta Ponchia, Annunziata Bruno, Asia Renzi, Claudia Landi, Enxhi Shaba, Francesca Paola Luongo, Alesandro Haxhiu, Paolo Giovanni Artini, Alice Luddi, Laura Governini, Paola Piomboni
Summary: Despite the widespread use of sperm cryopreservation, it can cause detrimental alterations in sperm function, including decreased viability, motility, and DNA fragmentation. Oxidative stress appears to play a prominent role in the mechanisms of human sperm cryodamage. Antioxidants such as myo-inositol have been shown to minimize the detrimental effects of reactive oxygen species (ROS) and improve sperm quality and motility. This study demonstrated that post-thaw addition of myo-inositol significantly improved sperm parameters, increased oxygen consumption, and decreased carbonyl groups, indicating reduced oxidative stress. These findings suggest that the developed sperm cryopreservation procedure may improve current in vitro techniques used for sperm cryostorage in assisted reproductive technology (ART) laboratories.
Article
Biochemistry & Molecular Biology
Ana Hernandez-Rodriguez, Carla Sousa-Santos, Fatima Gil, Elsa Cabrita, Pedro M. Guerreiro, Victor Gallego
Summary: During the spring of 2022, sperm samples were extracted from endangered leuciscid species found in Vasco da Gama aquarium facilities and rivers in Algarve region, Portugal. The study analyzed the sperm motion parameters of the four species and found similar kinetic patterns but varying longevity. Morphometric analysis showed that the spermatozoa had similar sizes and shapes. Short-term gamete storage and cryopreservation were also conducted, with acceptable results obtained in some species. This study provides valuable information for the management and conservation of these endangered species.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Stefano Paolo Marelli, Manuela Madeddu, Maria Grazia Mangiagalli, Silvia Cerolini, Luisa Zaniboni
Summary: This study compared the physical characteristics and fatty acid profiles of eggs produced in four different systems, showing significant differences in egg quality due to production system and outdoor space availability. Marketed eggs from different production systems were found to vary in fatty acid profile and physical parameters.
Article
Agriculture, Dairy & Animal Science
Alessandro Franzoni, Marta Gariglio, Annelisse Castillo, Dominga Soglia, Stefano Sartore, Arianna Buccioni, Federica Mannelli, Martino Cassandro, Filippo Cendron, Cesare Castellini, Alice Cartoni Mancinelli, Silvia Cerolini, Ahmad Abdel Sayed, Nicolaia Iaffaldano, Michele Di Iorio, Margherita Marzoni, Sonia Salvucci, Achille Schiavone
Summary: Loss of biodiversity is a global concern, with industrialized livestock farming and highly selected hybrid breeds leading to the extinction of many native chicken breeds. Despite the existence of niche markets, native chicken breeds have low diffusion rates in Italy. Encouraging the use of local breeds in alternative poultry farming could help protect biodiversity, utilizing consumer demand for alternative poultry products and high retail prices.
Article
Agriculture, Dairy & Animal Science
Annelisse Castillo, Marta Gariglio, Alessandro Franzoni, Dominga Soglia, Stefano Sartore, Arianna Buccioni, Federica Mannelli, Martino Cassandro, Filippo Cendron, Cesare Castellini, Alice Cartoni Mancinelli, Nicolaia Iaffaldano, Michele Di Iorio, Margherita Marzoni, Sonia Salvucci, Silvia Cerolini, Luisa Zaniboni, Achille Schiavone
Summary: The conservation status of native Italian poultry breeds is generally poor, with small population sizes and lack of awareness among poultry farmers and the general public. Promoting the virtues of Italian breeds would help encourage breeders to rear these birds and consumers to buy their products. Collaboration between university research centers, public entities, and breeders is essential for facilitating access to pure breed birds and improving the conservation status of native poultry breeds.
Article
Agriculture, Dairy & Animal Science
Valentina Bongiorno, Achille Schiavone, Manuela Renna, Stefano Sartore, Dominga Soglia, Paola Sacchi, Marta Gariglio, Annelisse Castillo, Cecilia Mugnai, Claudio Forte, Chiara Bianchi, Silvia Mioletti, Laura Gasco, Ilaria Biasato, Alberto Brugiapaglia, Federico Sirri, Marco Zampiga, Francesco Gai, Margherita Marzoni, Silvia Cerolini, Sihem Dabbou
Summary: This research investigated the slaughter performance and meat quality of two native Italian chicken breeds, Bionda Piemontese and Bianca di Saluzzo. The results showed that slaughtering at 7 months of age was associated with the best slaughter performance and meat quality characteristics in both breeds.
Article
Biology
Luisa Zaniboni, Manuela Madeddu, Fabio Mosca, Ahmad Abdel Sayed, Stefano Paolo Marelli, Michele Di Iorio, Nicolaia Iaffaldano, Silvia Cerolini
Summary: This study compared the effect of two permeant-cryoprotectants, DMA and NMA, at different concentrations on the quality and fertility of post-thaw rooster semen. Results showed that increasing the concentration of both DMA and NMA improved semen quality and fertility. Semen cryopreserved with DMA at a concentration of 6% had the best fertility and embryo viability, while semen cryopreserved with NMA at a concentration of 2% achieved the highest fertility. Therefore, the use of NMA is recommended for cryopreservation of chicken semen.
Article
Agriculture, Dairy & Animal Science
Stefano Paolo Marelli, Luisa Zaniboni, Maria Giuseppina Strillacci, Manuela Madeddu, Silvia Cerolini
Summary: This study investigated the phenotypical traits of two Italian heritage turkey breeds, Brianzolo and Nero d'Italia, through morphometry and colorimetric analysis. The study also highlighted the risk of genetic erosion and extinction faced by these breeds.
Article
Biology
Gianluca Paventi, Michele Di Iorio, Giusy Rusco, Anatoly P. Sobolev, Silvia Cerolini, Emanuele Antenucci, Mattia Spano, Luisa Mannina, Nicolaia Iaffaldano
Summary: Semen cryopreservation is crucial for preserving biodiversity, but in avian species like turkeys, the process leads to a significant reduction in sperm quality. Using NMR technology, this study analyzed the metabolic changes in turkey sperm during cryopreservation, revealing differences in the levels of amino acids, water-soluble compounds, and lipids between fresh and frozen-thawed samples.
Article
Veterinary Sciences
Manuela Madeddu, Stefano Marelli, Ahmad Abdel Sayed, Fabio Mosca, Silvia Cerolini, Luisa Zaniboni
Summary: More research in reproductive biology is needed to preserve endangered psittacine species. This study investigated the effects of different processing protocols on psittacines seminal quality analysis and found significant differences in the effectiveness of analysis protocols.
VETERINARY MEDICINE INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Manuela Madeddu, Luisa Zaniboni, Stefano Paolo Marelli, Stefano Comazzi, Silvia Cerolini
Summary: This study investigated the effect of two permeant-cryoprotectants, dimethy-lacetamide (DMA) and N-methylacetamide (NMA), at different concentrations (0, 2, 4, and 6%) on the quality of cryopreserved chicken semen. Sperm acrosome integrity and mitochondrial activity were assessed using flow cytometry, with only mitochondrial activity being significantly affected by the different cryoprotectant concentrations.
Article
Agriculture, Dairy & Animal Science
Stefano Paolo Marelli, Luisa Zaniboni, Manuela Madeddu, Maria Giuseppina Strillacci, Silvia Cerolini
Summary: The aim of this research is to study breeders' management strategies and their effects on reproductive performances in heritage breeds. A total of 468 litters were analyzed, and the results showed no effects of breeds on litter size, percentage of weaned kits, and percentage of selected kits. However, the year of birth had significant effects on litter size and percentage of weaned kits. The inclusion of external breeders did not significantly influence the analyzed reproductive data. The number of litters per buck and per doe were not influenced by breed.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Annelisse Castillo, Carla Lenzi, Andrea Pirone, Alessandro Baglini, Silvia Cerolini, Achille Schiavone, Margherita Marzoni Fecia di Cossato
Summary: This study evaluated the viability and mobility of rooster sperm, as well as the occurrence of normal cells and sperm injuries, in post-thawed pelleted sperm. Different pelleting protocols were compared, and it was found that the protocols using 6% DMA showed the highest values of thawed sperm mobility. The most favorable thawing methods were using the hot-plate at 60 degrees C or the water-bath at 50 degrees C. The best sperm quality parameters were achieved with a 1:2 dilution rate, a 30-min equilibration time at 4 degrees C, 6% DMA, and thawing 80 muL pellets using the water-bath at 50 degrees C or the hot-plate at 60 degrees C. Despite the damage caused by the freezing-thawing methodology, the survival rate of normal sperm cells reached 39%, with 32% recovered mobility compared to fresh sperm samples.
ITALIAN JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.