Article
Oncology
Catarina Nascimento, Andreia Gameiro, Joao Ferreira, Jorge Correia, Fernando Ferreira
Summary: Feline mammary carcinoma is the third most common neoplasia in cats, with highly malignant behavior. The study suggests that VEGF-A and its serum receptors assessment may have potential in the diagnosis and treatment of feline mammary carcinoma.
Article
Oncology
Maria Grazia Atzori, Claudia Ceci, Federica Ruffini, Manuel Scimeca, Rosella Cicconi, Maurizio Mattei, Pedro Miguel Lacal, Grazia Graziani
Summary: The study indicates that inhibiting the activation of VEGFR-1 by PlGF can effectively restrain the metastatic potential of melanoma, reducing tumor invasiveness and tropism toward bone tissue.
Review
Chemistry, Medicinal
Die Jiang, Ting Xu, Lei Zhong, Qi Liang, Yonghe Hu, Wenjing Xiao, Jianyou Shi
Summary: Angiogenesis is a vital biological process for body growth and development, wound healing, and tissue formation. Dysregulation of VEGFR signaling can lead to diseases, making it a crucial research area for disease treatment. Currently, anti-VEGF drugs used in ophthalmology have limitations, and there is a need to develop small molecule inhibitors of VEGFR for the targeted treatment of ocular neovascularization diseases.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Oncology
Lorenzo Fornaro, Gianna Musettini, Paola Orlandi, Irene Pecora, Caterina Vivaldi, Marta Banchi, Francesca Salani, Elisabetta Fini, Valentina Massa, Silvia Catanese, Federico Cucchiara, Monica Lencioni, Gianluca Masi, Enrico Vasile, Guido Bocci
Summary: Ramucirumab plus paclitaxel is considered standard treatment for second-line gastric carcinoma (GC) patients. This study evaluated plasma VEGF-A, VEGF-D, and sVEGFR-2 as potential markers of resistance or response to this treatment. Results showed significant increases in VEGF-A and VEGF-D levels during treatment, and early increase in sVEGFR-2 levels was associated with longer progression-free survival and overall survival.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Ivan Guryanov, Tatiana Tennikova, Arto Urtti
Summary: VEGFs play crucial roles in angiogenesis, but dysregulation can lead to pathological angiogenesis in various diseases. Monoclonal antibodies and decoy receptors are commonly used to neutralize VEGFA in anti-angiogenic therapies. Short peptides are promising alternatives to full-length VEGFA inhibitors, overcoming some limitations of existing therapies.
Review
Biochemistry & Molecular Biology
Peace Mabeta, Vanessa Steenkamp
Summary: The VEGF/VEGFR axis plays a crucial role in angiogenesis and tumor vascularization. Current therapies targeting VEGF and its receptors have shown limited effectiveness and undesirable off-target effects. Recent studies have identified VEGF splice variants that modulate angiogenesis, and their expression is regulated by cues within the tumor microenvironment. These variants challenge the established norm of VEGF signaling and their aberrant expression has been observed in several cancers, highlighting the need for further research on their role in cancer and their impact on antiangiogenic therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zhibao Wang, Bo Cao, Peng Ji, Fan Yao
Summary: Propofol inhibits tubular structure formation and various biological functions of tumor-associated endothelial cells, including migration, adhesion, proliferation, and survival, by suppressing the expression and secretion of multiple pro-angiogenic factors and specific signaling pathways. These findings provide preclinical evidence for the anti-cancer properties of propofol and its potential mechanisms in inhibiting tumor growth and metastasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Oncology
Laura Micheli, Carmen Parisio, Elena Lucarini, Alessia Vona, Alessandra Toti, Alessandra Pacini, Tommaso Mello, Serena Boccella, Flavia Ricciardi, Sabatino Maione, Grazia Graziani, Pedro Miguel Lacal, Paola Failli, Carla Ghelardini, Lorenzo Di Cesare Mannelli
Summary: Studies in murine models suggest that VEGF-A and its receptor VEGFR-1 play a crucial role in chemotherapy-induced neuropathic pain, mediating pain transmission through communication between astrocytes and neurons. Blocking VEGFR-1 may help alleviate neuropathic pain by disrupting this interaction.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Flavie Courtaut, Alessandra Scagliarini, Virginie Aires, Clarisse Cornebise, Jean-Paul Pais de Barros, Celine Olmiere, Dominique Delmas
Summary: The study explores potential targets for combating age-related macular degeneration and finds that a new nutritional supplement, Resvega, may effectively inhibit VEGF secretion, making it a promising complementary treatment against AMD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Samaneh Ghasemali, Safar Farajnia, Abolfazl Barzegar, Mohammad Rahmati-Yamchi, Roghayyeh Baghban, Leila Rahbarnia, Hamid R. Y. Nodeh
Summary: This review explores the latest developments in anti-angiogenic agents, emphasizing their role in cancer treatment. Research shows that anti-angiogenesis agents, particularly in combination therapy, are effective in treating various cancers.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Joanna Janusz, Aleksandra Janusz, Zdzislawa Kondera-Anasz, Justyna Sikora, Marta Smycz-Kubanska, Aleksandra Englisz, Dominika Wendlocha, Aleksandra Mielczarek-Palacz
Summary: Studies suggest that BMP-2 and BMP-7, along with their receptors ALK-1 and BMPR2, play important roles in the formation and development of endometriosis. Concentrations of these factors in peritoneal fluid showed a significant decrease in BMP-2 and an increase in BMP-7, ALK-1, and BMPR2 in women with endometriosis compared to the reference group, indicating more effective formation of new blood vessels especially in the early stages of the disease.
Article
Pharmacology & Pharmacy
Reem M. Hazem, Anhar A. Mohamed, Nagat Ghareb, Eman T. Mehanna, Noha M. Mesbah, Dina M. Abo-Elmatty, Mohamed Saleh Elgawish
Summary: This study investigated the anti-cancer role of two novel compounds, benzoimidazoquinazoline and benzimidazotriazin, which act as potent anti-angiogenic agents by inhibiting VEGF signaling pathways in breast cancer. The compounds reduced tumor weight, VEGF levels, and down-regulated VEGFR2 and CD34 expression in tumor tissue, offering a promising strategy for chemotherapy advancement in breast cancer treatment.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Christina M. Termini, Amara Pang, Tiancheng Fang, Martina Roos, Vivian Y. Chang, Yurun Zhang, Nicollette J. Setiawan, Lia Signaevskaia, Michelle Li, Mindy M. Kim, Orel Tabibi, Paulina K. Lin, Joshua P. Sasine, Avradip Chatterjee, Ramachandran Murali, Heather A. Himburg, John P. Chute
Summary: Ionizing radiation and chemotherapy deplete hematopoietic stem cells and damage the vascular niche, leading to the secretion of SEMA3A from bone marrow endothelial cells. Inhibition of NRP1 promotes vascular and hematopoietic regeneration through increased expression of R spondin 2, suggesting a potential therapeutic target for improving hematopoietic recovery after myeloablation.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Edward Hartsough, Rajani R. J. Shelke, Razie Amraei, Zahra Aryan, Saran Lotfollahzadeh, Nader Rahimi
Summary: Activation and signal transduction of vascular endothelial growth factor receptor-2 (VEGFR-2) play crucial roles in vasculogenesis and pathological angiogenesis. The study reveals that protein arginine methyltransferase 4 (PRMT4) mediates methylation of a specific site on VEGFR-2, which affects VEGFR-2-induced angiogenic responses. This finding may have implications for the development of targeted VEGFR-2 inhibitors.
Article
Ophthalmology
Jooseppi Puranen, Sanna Koponen, Tiina Nieminen, Iiris Kanerva, Emmi Kokki, Pyry Toivanen, Arto Urtti, Seppo Yla-Herttuala, Marika Ruponen
Summary: This study evaluated the efficacy and safety of gene therapy for inhibiting ocular neovascularization. The results showed that gene therapy effectively reduced the formation of new blood vessels and could potentially be used as a therapeutic tool for retinal diseases associated with neovascularization.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
M. M. Kaczmarek, Z. P. Reliszko, J. Szuszkiewicz, A. Nitkiewicz, M. M. Guzewska, K. Myszczynski, M. Romaniewicz, M. Sikora, M. Kajko, Y. Heifetz
Summary: Circulating miRNAs serve as potential indicators of reproductive status in pigs, with distinct profiles found in serum samples collected from cyclic and pregnant animals. The study identified reproductive status-dependent miRNAs involved in processes important during the estrous cycle and early pregnancy, highlighting their potential as biomarkers for reproductive health and breeding management.
Article
Immunology
Krzysztof Lukaszuk, Jolanta Kiewisz, Karolina Rozanska, Malgorzata Dabrowska, Amira Podolak, Grzegorz Jakiel, Izabela Woclawek-Potocka, Aron Lukaszuk, Lukasz Rabalski
Summary: The study aimed to compare anti-SARS-CoV-2 test results to evaluate the use of the WHO standard unit for measuring vaccination responses. The three assays showed varying correlations at different time points, indicating the need for further work on international standard measurements for SARS-CoV-2 Ig.
Article
Biochemistry & Molecular Biology
Michal Trela, Olga Witkowska-Pilaszewicz, Dominika Domanska, Monika M. Kaczmarek, Bartosz Pawlinski, Zdzislaw Gajewski, Malgorzata Domino
Summary: This study evaluated the effectiveness of vaginal progesterone inserts therapy in cows with anestrus type II. The expression of progesterone and estrogen receptors in the endometrium was assessed, and blood levels of estradiol and progesterone were evaluated. The results showed a decrease in mRNA expression of certain receptors in both treated and untreated animals. However, treated animals showed an increase in the expression of specific receptors compared to untreated animals. In addition, the recurrence of ovarian cyclicity and pregnancy rates were higher in the treated group.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Jolanta Kiewisz, Anna Pawlowska, Agata Winiarska, Agnieszka Perkowska-Ptasinska, Agnieszka Skowronska, Janusz Godlewski, Zbigniew Kmiec, Tomasz Stompor
Summary: The observed increase in serum WNT4 protein in the early stages of CKD and in patients diagnosed with immune-mediated glomerular disease may suggest that WNT4 may act as a mediator of inflammation.
ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE
(2022)
Article
Immunology
Krzysztof Lukaszuk, Amira Podolak, Grzegorz Jakiel, Jolanta Kiewisz, Izabela Woclawek-Potocka, Aron Lukaszuk, Lukasz Rabalski
Summary: The study confirmed that a half-dose vaccination schedule can produce comparable antibody responses in adults as the recommended full dose, which may help achieve widespread vaccination coverage more quickly and cheaply.
Article
Medicine, General & Internal
Krzysztof Lukaszuk, Grzegorz Jakiel, Izabela Woclawek Potocka, Jolanta Kiewisz, Jolanta Olszewska, Wlodzimierz Sieg, Amira Podolak, Ewa Pastuszek, Artur Wdowiak
Summary: ICSI is a commonly used treatment for oocyte fertilization, and the quality of sperm selection depends on accurate visualization of morphology. We emphasize the importance of optical system's resolution in observing sperm and provide specific microscope system setup recommendations. Furthermore, a lack of standardization in microscopy techniques for both ICSI and IMSI may contribute to mixed research results.
Article
Reproductive Biology
Joanna Szuszkiewicz, Anna Nitkiewicz, Klaudia Drzewiecka, Monika M. Kaczmarek
Summary: Embryo implantation is the most critical stage of pregnancy, dependent on the synchronized developmental capacity of the embryo and uterine receptivity. In early pregnancy, both the conceptus and uterus release various factors to facilitate successful implantation and placenta formation. MicroRNAs, which regulate gene expression, are considered important regulators of early pregnancy events.
BIOLOGY OF REPRODUCTION
(2022)
Article
Multidisciplinary Sciences
Andrzej Ciereszko, Mariola A. Dietrich, Mariola Slowinska, Joanna Nynca, Michal Ciborowski, Monika M. Kaczmarek, Kamil Myszczynski, Joanna Kisluk, Anna Majewska, Anna Michalska-Falkowska, Natalia Kodzik, Joanna Reszec, Ewa Sierko, Jacek Niklinski
Summary: Proteomics was used to study non-small cell lung cancer and investigate the differences in proteins between the tumor center and tumor margin. Several important proteins related to lung cancer development and molecular specificity were identified, which could potentially serve as biomarkers and targets for anticancer therapy.
Article
Immunology
Jolanta Kiewisz, Damian Drzyzga, Karolina Rozanska, Emilia Krzynowek, Krzysztof Lukaszuk
Summary: This study measured the antibody titers of healthcare workers who received two doses of BNT162b2 vaccines, considering their prior history of SARS-CoV-2 infection. The results showed that individuals with previous infection had higher antibody titers. The highest antibody level was observed 8 days after the second dose of the vaccine. The occurrence of local and systemic adverse reactions appeared to be independent of the vaccine dose. These findings can contribute to the improvement of vaccination programs and management of vaccine-related adverse effects.
Article
Biochemistry & Molecular Biology
Joanna Szuszkiewicz, Kamil Myszczynski, Zaneta P. Reliszko, Yael Heifetz, Monika M. Kaczmarek
Summary: Research has shown that during early pregnancy, communication between the embryo and uterus occurs through the exchange of extracellular vesicles carrying miRNAs. These vesicles impact embryonic development and trophoblast cell function and interactions, playing a crucial role in successful embryo implantation and pregnancy.
Article
Biochemistry & Molecular Biology
Pawel Likszo, Katarzyna Gromadzka-Hliwa, Jan Klos, Monika M. Kaczmarek, Adam J. Ziecik
Summary: This study focuses on the routine procedure of estrous cycle synchronization in pigs and the use of gonadotropins to stimulate ovarian activity. The research reveals the importance of sexual maturity in maintaining the estrogenic environment and the moderate role of eCG and FSH in activating steroidogenic enzymes in preovulatory follicles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Maria M. Guzewska, Joanna Szuszkiewicz, Monika M. Kaczmarek
Summary: The establishment of cell-to-cell communication is crucial for successful mammalian pregnancy. Extracellular vesicles (EVs) carrying unique molecular cargo have been identified as mediators of this communication during early pregnancy. However, the mechanism of EV-mediated crosstalk between early embryos and the endometrium needs further investigation. Understanding the role of EVs in mammalian reproduction can greatly enhance our knowledge of successful reproductive performance.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2023)
Article
Oncology
Jolanta Kiewisz, Tomasz Wasniewski, Jacek Kiezun, Agnieszka Skowronska, Monika M. Kaczmarek, Blazej Szostak, Anna E. Kowalczyk, Zbigniew Kmiec
Summary: This study aimed to evaluate the alterations of WNT4 gene expression and protein immunoreactivity in endometrial cancer (EC) and their association with tumor characteristics, clinicopathological factors, and estrogen dependence. The results showed that WNT4 gene expression was decreased in EC samples, particularly in endometrioid EC, and was associated with tumor characteristics. However, there was no statistical difference in WNT4 protein immunoreactivity or estrogen receptor alpha immunoreactivity between EC and control group. The study also found that alterations in WNT4 gene expression tended to be mutually exclusive with alterations in ESR1 gene expression. Overall, this study highlights the importance of evaluating the molecular background of EC, and suggests that WNT4 gene may be a marker of functional estrogen signaling in endometrioid EC.
Review
Biochemistry & Molecular Biology
Wlodzimierz Sieg, Jolanta Kiewisz, Amira Podolak, Grzegorz Jakiel, Izabela Woclawek-Potocka, Jakub Lukaszuk, Krzysztof Lukaszuk
Summary: Inflammatory processes during embryo implantation play a crucial role in the successful establishment of pregnancy. However, excessive inflammation can lead to pregnancy complications. Understanding these mechanisms can bring new hope to infertile couples for improved treatments.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Amira Podolak, Joanna Liss, Jolanta Kiewisz, Sebastian Pukszta, Celina Cybulska, Michal Rychlowski, Aron Lukaszuk, Grzegorz Jakiel, Krzysztof Lukaszuk
Summary: This study investigated the correlation between mitochondrial DNA content in embryos and their developmental potential. The findings showed that aneuploid embryos had a higher mitochondrial DNA content compared to euploid embryos.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.
