Article
Food Science & Technology
Kungnang Bunsroem, Witoon Prinyawiwatkul, Siwatt Thaiudom
Summary: This study evaluated the susceptibility of whey proteins to enzyme hydrolysis and the antidiabetic properties of protein hydrolysates. The results showed that alpha-LA hydrolysate was more effective than beta-LG hydrolysate in inhibiting DPP-W activity and increasing GLP-1 levels. Heat treatment at 75 degrees C had the greatest impact on the antidiabetic properties of the protein hydrolysates.
Article
Food Science & Technology
Maria Romo, Massimo Castellari, Dinar Fartdinov, Xavier Felipe
Summary: In this study, high-pressure processing was used to remove beta-Lactoglobulin from bovine native whey concentrate and generate an enriched alpha-Lactalbumin fraction. The optimal conditions for alpha-Lactalbumin yield and purification degree were determined. The results showed that high-pressure processing could improve the precipitation of beta-Lactoglobulin and the purification of alpha-Lactalbumin. Further characterization and application of membrane concentration and high-pressure processing are recommended for the valorization of cheese whey.
Article
Agriculture, Dairy & Animal Science
Kamila Puppel, Patrycja Staniszewska, Marcin Golebiewski, Jan Slosarz, Grzegorz Grodkowski, Pawel Solarczyk, Malgorzata Kunowska-Slosarz, Piotr Kostusiak, Beata Kuczynska, Tomasz Przysucha
Summary: This study demonstrates a relationship between whey protein concentrations and blood BHBA levels, serving as markers for diagnosing metabolic diseases. Differences in alpha-lactoalbumin and beta-lactoglobulin concentrations in milk were observed based on varying BHBA levels in cows.
Article
Food Science & Technology
Maria Romo, Massimo Castellari, Ricard Bou, Pere Gou, Xavier Felipe
Summary: Whey from goat and sheep are being studied for their nutritional properties, but the presence of beta-Lg in these whey sources may cause allergies in infants. Therefore, researchers are interested in developing whey ingredients with higher alpha-La/beta-Lg ratios. This study investigated the effect of high-pressure processing on goat and sheep native whey concentrates (NWC) to obtain alpha-La-enriched fractions. The results showed that high-pressure processing reduced the concentration of beta-Lg in NWC, while alpha-La concentration remained unchanged.
Article
Engineering, Chemical
Marlen Gonzalez-Amado, Ana P. M. Tavares, Mara G. Freire, Ana Soto, Oscar Rodriguez
Summary: This study investigated the use of Aqueous Two-Phase Systems (ATPS) for the recovery of lactose, BSA, beta-lactoglobulin and alpha-lactalbumin from cheese whey. Different ATPS were studied for partitioning the selected solutes, showing efficient recovery of proteins and lactose under optimized conditions. This polymer/salt ATPS method could be a scalable and cost-effective separation strategy for cheese whey and related wastes.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Applied
Clara Fucinos, Natalia Estevez, Lorenzo Pastrana, Clara A. Tovar, Maria Luisa Rua
Summary: The bioactivity of α-lactalbumin was significantly improved by the synthesis of nanotubes, with higher ACE-inhibitory and antioxidant activity. Different ion ratios during nanotube synthesis altered the microstructure of α-lactalbumin nanotubes, resulting in varied rheological properties and structural regeneration capabilities.
FOOD HYDROCOLLOIDS
(2021)
Article
Food Science & Technology
Serena Boscaini, Peter Skuse, Kanishka N. Nilaweera, John F. Cryan, Paul D. Cotter
Summary: This review provides an overview of the beneficial effects of whey protein (WP) and its components on human health, particularly in controlling obesity and its comorbidities. WP consumption positively impacts energy balance and obesity-related parameters, primarily through modulation in adipose tissue metabolism and changes that occur in the gastrointestinal tract. Furthermore, WP also has a positive impact on gut microbiota, mood, and behavior. This review emphasizes the importance of WP supplementation in preventing or treating obesity and highlights areas for further research.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2023)
Review
Chemistry, Analytical
Jelena Radosavljevic, Dragana Stanic-Vucinic, Marija Stojadinovic, Mirjana Radomirovic, Ana Simovic, Milica Radibratovic, Tanja Cirkovic Velickovic
Summary: Whey protein, an important source of nutrition and biotechnology, is considered a by-product of the dairy industry due to low economic value. The challenge in whey protein separation lies in low concentration and similar properties. Ion exchange techniques are widely used for separation, and recent research on ion exchange membranes shows promise for industrial application. Adsorption-based methodologies targeting protein structure differences are anticipated to further improve whey protein separation.
CURRENT ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Davide Pedrali, Alessio Scarafoni, Anna Giorgi, Vera Lavelli
Summary: Encapsulation of antioxidants in hydrogels can increase their stability and bioaccessibility. Hydrogels of alginate and whey proteins provide a pH-dependent dissolution mechanism, allowing the release of encapsulated compounds in the intestine for absorption.
Article
Food Science & Technology
Lina Zhang, Ruoya Zhou, Jinyue Zhang, Peng Zhou
Summary: Whey proteins, as the second most abundant protein in milk, contain many bioactive proteins that play important roles in consumer health. Heating treatments during dairy processing can lead to changes in the structure and bioactivity of whey proteins, which may impact the nutritional value and properties of dairy products.
INTERNATIONAL DAIRY JOURNAL
(2021)
Article
Food Science & Technology
Yu-Zhe Gao, Hong-Hua Xu, Chen Guan, Rui-Chi Guo, Xiao-Tong Yang, Cai-Hong Ma, Mei-Li Shao
Summary: This study found that monomers, homogeneous nuclei, and protofibrils have different abilities to resist disturbance by other protein agents during beta-lactoglobulin fibril formation. Coassembly of beta-lg monomers with α-lactalbumin/κ-casein affected fibril formation differently compared to coassembly with homogeneous nuclei and protofibrils. α-lactalbumin increased fibril diameter, while κ-casein clearly inhibited fibril formation.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2021)
Review
Food Science & Technology
Jianxin Xiao, Jiaying Ma, Muhammad Zahoor Khan, Gibson Maswayi Alugongo, Tianyu Chen, Shuai Liu, Shengli Li, Zhijun Cao
Summary: Whey protein components have anticancer attributes and can reduce the risk of colorectal cancer. They have anti-inflammatory and antioxidant abilities and can induce apoptosis, inhibit tumor cell proliferation, and impede metastasis. Studies have shown the importance of incorporating whey protein components into a balanced daily diet for the prevention and treatment of colorectal cancer.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Applied
Tai Zhang, Yisuo Liu, Pengjie Wang, Yixuan Li, Fazheng Ren, Huaxi Yi
Summary: The interaction between alpha-La and beta-Lg promotes the formation of aggregates in dUHT milk during heat treatment and storage, which is important for optimizing the process and storage of dUHT milk.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Applied
Thomas Valente De Oliveira, Marcelo Depolo Poleto, Samuel Vieira Barbosa, Jane Selia dos Reis Coimbra, Eduardo Basilio De Oliveira
Summary: Computational molecular dynamics was used to analyze the molecular conformations of bovine alpha-lactalbumin at different temperatures, showing that the absence of Ca2+ triggered significant protein conformation changes and affected the foaming properties. This study demonstrates the power of MD in providing insights into the molecular origins of food protein functionalities.
Article
Nutrition & Dietetics
Dagmara Zlotkowska, Mateusz Kuczynski, Ewa Fuc, Joanna Fotschki, Barbara Wroblewska
Summary: This study aimed to evaluate the effect of glycation on the ex vivo response of beta-lactoglobulin sensitized lymphocytes. Results showed that glycation does not reduce the immunogenicity of the protein.
Meeting Abstract
Oncology
O. Kacar, I. Hatipoglu, V. Yilmaz, N. Arda, E. Ulukaya, C. A. Acilan
EUROPEAN JOURNAL OF CANCER
(2016)
Meeting Abstract
Oncology
C. Acilan, B. Cevatemre, Z. Adiguzel, D. Karakas, E. Ulukaya, N. Arda, N. Ribeiro, I. Correiae, J. C. Pessoa
EUROPEAN JOURNAL OF CANCER
(2016)
Meeting Abstract
Oncology
Z. Adiguzel, S. Ozalp-Yaman, G. Celik, S. Salem, T. Bagci-Onder, Y. C. Cetin, N. Arda, C. Acilan
EUROPEAN JOURNAL OF CANCER
(2016)
Meeting Abstract
Oncology
E. Onay Ucar, M. Pekmez, E. Mertoglu, L. Dalyan, N. Arda
EUROPEAN JOURNAL OF CANCER
(2016)
Article
Agronomy
Yahya Emin Demirci, Cihan Inan, Aslihan Gunel, Dilara Maytalman, Zafer Mert, A. Tarik Baykal, Senay Vural Korkut, Nazli Arda, Semra Hasancebi
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2016)
Article
Cell Biology
Selda Gezginci-Oktayoglu, Evren Onay-Ucar, Serap Sancar-Bas, Ayse Karatug-Kacar, Emine S. N. Arda, Sehnaz Bolkent
JOURNAL OF CELLULAR PHYSIOLOGY
(2018)
Article
Physiology
Farinaz Jafari Ghods, Aysegul Topal Sarikaya, Nazli Arda, Vedat Hamuryudan
KIDNEY & BLOOD PRESSURE RESEARCH
(2017)
Article
Chemistry, Multidisciplinary
Peyker Kayce, Nazli Boke Sarikahya, Murat Pekmez, Nazli Arda, Suheyla Kirmizigul
TURKISH JOURNAL OF CHEMISTRY
(2017)
Article
Chemistry, Multidisciplinary
Aysecik Kacmaz, Nahide Gulsah Deniz, Serdar Goksin Aydinli, Cigdem Sayil, Evren Onay-Ucar, Elif Mertoglu, Nazli Arda
Article
Nanoscience & Nanotechnology
Oguz Yucel, Aslihan Sengelen, Serkan Emik, Evren Onay-Ucar, Nazli Arda, Gulten Gurdag
Article
Allergy
Behnaz Karadogan, Sengul Beyaz, Asli Gelincik, Suna Buyukozturk, Nazli Arda
Summary: This study aimed to evaluate the differences in oxidative stress biomarkers and antioxidant capacity between asthma patients and healthy individuals. The results showed a significant increase in levels of malondialdehyde and protein carbonyls, as well as a decrease in reduced glutathione levels, in asthma patients. These findings suggest the presence of severe oxidative stress in asthma patients even under controlled conditions.
Article
Chemistry, Analytical
Nazli Boke Sarikahya, Gaye Sumer Okkali, Veysel U. Celenk, Elif Mertoglu, Murat Pekmez, Nazli Arda, Gulacti Topcu, Ahmet C. Goren
Summary: The study aimed to determine the chemical composition and biological activities of ethanol and n-hexane extracts of three different Jurinea species. Results showed that certain extracts exhibited cytotoxic effects on cancer cells and high antioxidant activities. These plants may be considered as alternative sources of cytotoxic and/or antioxidant flavonoids, phenolics, and unsaturated fatty acids.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Allergy
Behnoush Nasr Zanjani, Afshin Samadi, Selen Yilmaz Isikhan, Incilay Lay, Sengul Beyaz, Asli Gelincik, Suna Buyukozturk, Nazli Arda
Summary: This study aimed to compare the levels of oxysterols and lipid peroxidation products in allergic asthma patients and healthy controls, and provide information about the involvement of lipid peroxidation in allergic asthma. The results showed that plasma levels of oxysterols and lipid peroxidation products were significantly higher in allergic asthma patients compared to healthy controls, suggesting their involvement in the pathogenesis of allergic asthma.
Article
Allergy
Demirhan Cetereisi, Nurgul Karlioglu, Ash Gelincik, Sylvie Michelland, Evren Onay-Ucar, Belkis Ertek, Nilgun Akdeniz, Sacide Erden, Bahattin Colakoglu, Unal Akkemik, Gunnur Deniz, Murat Pekmez, Helene Flamant-Waret, Sylvia Lehmann, Sandrine Bourgoin-Voillard, Michel Seve, Suna Buyukozturk, Nazli Arda
ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Omer Kacar, Buse Cevatemre, Ibrahim Hatipoglu, Nazli Arda, Engin Ulukaya, Veysel T. Yilmaz, Ceyda Acilan
BIOORGANIC & MEDICINAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
