Article
Plant Sciences
Yubo Yao, Hongmei Yuan, Guangwen Wu, Chunmei Ma, Zhenping Gong
Summary: This study investigated the effect of phosphorus on soybean nodule nitrogen fixation. It was found that phosphorus stress induced structural changes in ribosomal proteins, altered protein synthesis profiles, and up-regulated expression of peroxidase. Phosphorus transporter and purple acid phosphatase were also up-regulated to maintain phosphorus balance and nitrogen fixation function in phosphorus-stressed root nodules.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Wei Fan, Chunjiao Xia, Shixiang Wang, Jing Liu, Lijun Deng, Shiyong Sun, Xuelu Wang
Summary: The symbiotic relationship between legumes and rhizobia leads to the formation of nitrogen-fixing root nodules. This study reveals that infected cells and uninfected cells in the developing nodules both have 4C nuclei, and the 4C cells primarily invaded by rhizobia undergo endoreduplication. Moreover, the function of the 4C cells in the nodules changes at different time points after infection.
Article
Multidisciplinary Sciences
Barney A. Geddes, Jason V. S. Kearsley, Jiarui Huang, Maryam Zamani, Zahed Muhammed, Leah Sather, Aakanx K. Panchal, George C. diCenzo, Turlough M. Finan
Summary: The symbiosis between leguminous plants and rhizobia in root nodules plays a key role in sustainable agricultural systems. Research has identified a minimal bacterial gene complement necessary for successful symbiosis and nitrogen fixation, paving the way for engineering symbiosis in nonlegume crops. By reorganizing the minimal symbiotic genes into three modules, researchers have provided a platform for expanding symbiosis to other plant partners and identifying genetic components that modulate competitiveness for nodulation and the effectiveness of rhizobia-plant symbioses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Miroslava Hrbackova, Ivan Luptovciak, Katerina Hlavackova, Petr Dvorak, Michaela Ticha, Olga Samajova, Dominik Novak, Hanna Bednarz, Karsten Niehaus, Miroslav Ovecka, Jozef Samaj
Summary: Transgenic manipulation of SIMK in alfalfa plants infected with Sinorhizobium meliloti showed that downregulation of both SIMKK and SIMK led to reduced root hair growth and lower capacity for infection thread and nodule formation, while overexpression of GFP-tagged SIMK promoted root hair growth and clustering of infection threads and nodules. These findings suggest that genetic manipulations affecting SIMK expression have significant impacts on root hair, nodule, and shoot formation patterns in alfalfa, indicating the biotechnological potential of this MAPK.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Longlong Wang, Jianjun Liang, Yu Zhou, Tao Tian, Baoli Zhang, Deqiang Duanmu
Summary: In this study, 13 carbonic anhydrase (CA) genes were identified in the model legume Lotus japonicus, with three of them further characterized for their expression patterns during nodule development. Knockout experiments using CRISPR/Cas9 technology showed that the knocked-out genes were not essential for nitrogen fixation under normal symbiotic conditions. The diverse expression patterns and distributions of these genes suggest their potential functions in symbiotic nitrogen fixation, awaiting further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Agnes Csiszar, Daniel Winkler, Denes Bartha, Gergely Zagyvai
Summary: The black locust is an important invasive woody species known for its strong nitrogen-fixing ability. This study found a negative correlation between the number and biomass of root nodules of black locust and species richness, suggesting a negative impact of its nitrogen-fixing bacteria on biodiversity. Additionally, the biomass of root nodules also decreased with stand age.
Article
Plant Sciences
Bethany L. Holland, Megan L. Matthews, Pedro Bota, Lee J. Sweetlove, Stephen P. Long, George C. Dicenzo
Summary: Efforts are being made to bioengineer nitrogen fixation into nonleguminous crops, requiring a quantitative understanding of its energetic costs and the relationship between metabolic variations and symbiotic efficiency. A whole-plant metabolic model for soybean with its associated microsymbiont was developed and used to predict the cost-benefit of nitrogen fixation under different soil nitrogen availability. The model predicted a nitrogen-fixation cost of approximately 4.13 g C g(-1) N and a grain yield reduction of 27%, compared to non-nodulating plants receiving their nitrogen from the soil.
Review
Plant Sciences
Suvriti Sharma, Jahanvi Ganotra, Jyotipriya Samantaray, Ranjan Kumar Sahoo, Deepak Bhardwaj, Narendra Tuteja
Summary: A comprehensive understanding of nitrogen signaling cascades involving heterotrimeric G-proteins and their putative receptors can assist in the production of nitrogen-efficient plants.
Article
Plant Sciences
Laurent Sauviac, Antoine Remy, Emeline Huault, Melanie Dalmasso, Theophile Kazmierczak, Marie-Francoise Jardinaud, Ludovic Legrand, Corentin Moreau, Bryan Ruiz, Anne-Claire Cazale, Sophie Valiere, Benjamin Gourion, Laurence Dupont, Veronique Gruber, Eric Boncompagni, Eliane Meilhoc, Pierre Frendo, Florian Frugier, Claude Bruand
Summary: This study identified hundreds of plant and bacterial genes associated with nodule senescence through dual plant-bacteria RNA sequencing, providing a valuable resource for exploring the mechanisms of nodule senescence. The study revealed that genes related to both plant and bacterial cell cycle and stress responses play important roles in nodule senescence, suggesting complex regulatory networks involved in this process.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Soil Science
Silmar Primieri, Julio Cesar Pires Santos, Pedro Madeira Antunes
Summary: The tripartite symbiosis of Mimosa scabrella, N-2 fixing bacteria, and AMF can synergistically promote plant biomass and N-2 fixation, while nodule-associated bacteria may have a detrimental effect on this tripartite mutualism.
SOIL BIOLOGY & BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Melanie Gasser, Nicole Alloisio, Pascale Fournier, Severine Balmand, Ons Kharrat, Joris Tulumello, Lorena Carro, Abdelaziz Heddi, Pedro Da Silva, Philippe Normand, Petar Pujic, Hasna Boubakri
Summary: This study monitored the early stages of actinorhizal symbiosis at the transcriptional level and found that 42 genes were significantly upregulated in inoculated roots. Most of these genes are involved in biological processes induced during microbial infection. A non-specific lipid transfer protein (nsLTP) was found to play a role in antimicrobial defense and bind to specialized cells for nitrogen fixation. This study provides insights into the plant immune response and defense mechanisms against Frankia alni infection.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Biochemical Research Methods
Angus E. Rae, Vivien Rolland, Rosemary G. White, Ulrike Mathesius
Summary: The formation of infection threads in symbiotic infection of rhizobacteria in legumes is a unique and fascinating process, but poorly understood. New imaging methods have successfully visualized infection threads and potentially discovered a way to visualize the infection thread matrix.
Editorial Material
Plant Sciences
Carole Laffont, Florian Frugier
Summary: C-terminally encoded peptides (CEP) play a role in regulating nitrogen acquisition pathways in plants. Recent studies in Medicago truncatula have shown that root-produced CEP peptides control the symbiotic competence of rhizobia through the activity of the CRA2 receptor in shoots.
Article
Microbiology
A. A. Vladimirova, R. S. Gumenko, E. S. Akimova, Al. Kh. Baymiev, An. Kh. Baymiev
Summary: The study analyzed the functional activity of different phylogenetic variants of the transcriptional activator of nitrogen fixation genes in root nodule bacteria cells of different taxa. It was found that introducing an additional active copy of the nifA gene into the genome of these bacteria resulted in detectable nitrogenase activity, regardless of the phylogeny of the introduced nifA gene. This suggests the possibility of combinatorial processes in the evolution of nitrogen fixation genes.
Article
Plant Sciences
Paula Belles-Sancho, Yilei Liu, Benjamin Heiniger, Elia von Salis, Leo Eberl, Christian H. Ahrens, Nicola Zamboni, Aurelien Bailly, Gabriella Pessi
Summary: Rhizobia control the expression of auxin synthesis genes through NifA, leading to increased auxin levels in non-fixing mutant strains, which enhances root infection rates and plays a role in early symbiotic interactions.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Joao P. Monteiro, Felisa Rey, Tania Melo, Ana S. P. Moreira, Jean-Francois Arbona, Jorunn Skjermo, Silje Forbord, Jon Funderud, Diogo Raposo, Philip D. Kerrison, Marie-Mathilde Perrineau, Claire Gachon, Pedro Domingues, Ricardo Calado, M. Rosario Domingues
Article
Veterinary Sciences
Thomas R. De Ridder, Justine E. Campbell, Cheryl Burke-Schwarz, David Clegg, Emily L. Elliot, Samuel Geller, Wendy Kozak, Stephen T. Pittenger, Jennifer B. Pruitt, Jocelyn Riehl, Julie White, Melissa L. Wiest, Chad M. Johannes, John Morton, Pamela D. Jones, Peter F. Schmidt, Victoria Gordon, Paul Reddell
Summary: The study demonstrates that tigilanol tiglate is effective and well tolerated for the local treatment of mast cell tumors in dogs.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2021)
Article
Veterinary Sciences
Pamela D. Jones, Justine E. Campbell, Graham Brown, Chad M. Johannes, Paul Reddell
Summary: A study evaluated the durability of the treatment response achieved with Tigilanol tiglate in dogs with mast cell tumors in the U.S. by assessing MCT recurrence at the treatment site 6 and 12 months after treatment. Results showed that 89% of evaluable dogs remained tumor free at the treatment site by 12 months, with recurrences mostly occurring within the first 6 months.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2021)
Article
Veterinary Sciences
Paul Reddell, Thomas R. De Ridder, John M. Morton, Pamela D. Jones, Justine E. Campbell, Graham Brown, Chad M. Johannes, Peter F. Schmidt, Victoria Gordon
Summary: Following treatment with TT, wound area is related to pretreatment tumor volume, and healing time depends on wound area and body location of wounds.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Jason K. Cullen, Glen M. Boyle, Pei-Yi Yap, Stefan Elmlinger, Jacinta L. Simmons, Natasa Broit, Jenny Johns, Blake Ferguson, Lidia A. Maslovskaya, Andrei Savchenko, Paul Malek Mirzayans, Achim Porzelle, Paul Bernhardt, Victoria A. Gordon, Paul W. Reddell, Alberto Pagani, Giovanni Appendino, Peter G. Parsons, Craig M. Williams
Summary: The study challenges the longstanding view of some Protein Kinase C (PKC) isoforms as oncogenes, suggesting that activation of certain isoforms may have tumor-suppressive effects. This research identified a group of novel phorboids related to a PKC-activating compound, with potential anticancer activity. The findings indicate that PKC activation could be a promising strategy for anticancer drug discovery in some cases.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Babacar Thioye, Herve Sanguin, Aboubacry Kane, Cheikh Ndiaye, Dioumacor Fall, Diaminatou Sanogo, Robin Duponnois, Sergio Miana de Faria, Samba Ndao Sylla, Amadou Ba
Summary: Research on the use of arbuscular mycorrhizal fungi as biofertilizers in fruit tree orchards showed significant positive effects on growth and fruit production of jujube trees, lasting up to 18 months with potential to improve survival rates.
Article
Environmental Sciences
Gustavo Wyse Abaurre, Jorge Makhlouta Alonso, Orivaldo Jose Saggin Junior, Sergio Miana de Faria
Summary: This study evaluated the initial growth and tolerance to water stress of Samanea saman seedlings produced with different substrates and inoculation patterns. Inoculation promoted higher height and biomass in seedlings produced in the standard substrate, while roots biomass decreased in the sludge when fertilized or inoculated. Seedlings grown in sludge showed higher height and biomass before planting, but after rehydration, the height increment was similar for the sludge and the standard substrate.
Article
Microbiology
Jerri Edson Zilli, Camila Pereira de Moraes Carvalho, Aline Vieira de Matos Macedo, Luis Henrique de Barros Soares, Eduardo Gross, Euan Kevin James, Marcelo Fragomeni Simon, Sergio Miana de Faria
Summary: The neotropical genus Calliandra is important for ecology and agroforestry, but little is known about its nodulation or rhizobia. Through studying species from different regions, it was found that Calliandra nodules have typical anatomy but symbionts vary according to geographical origin, affecting the species' preferences and symbiotic relationships.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Plant Sciences
Giuseppina Chianese, Hawraz Ibrahim M. Amin, Chiara Maioli, Paul Reddell, Peter Parsons, Jason Cullen, Jenny Johns, Herlina Handoko, Glen Boyle, Giovanni Appendino, Orazio Taglialatela-Scafati, Simone Gaeta
Summary: The kernels of the Australian blushwood tree contain a high concentration of phorboids, which can be used to produce the anticancer drug tigilanol tiglate. Unlike croton oil, the oily matrix of the blushwood kernels mainly consists of free fatty acids. By active partitioning, a hidden tigliane fraction was recovered and characterized for the first time. The esterification profile of the epoxytigliane di- and triesters differs, and certain esters showed cytotoxic activity.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Biochemistry & Molecular Biology
Shahida A. Mitu, Praphaporn Stewart, Trong D. Tran, Paul W. Reddell, Scott F. Cummins, Steven M. Ogbourne
Summary: This study identified potential gene biomarkers associated with biosynthesis of EBC-46, which may be used in the selective breeding of F. picrosperma. High-EBC-46-producing plants were found to have differentially expressed genes related to defense, transport, and biosynthesis compared to low producers.
Article
Plant Sciences
Sergio M. de Faria, Jens J. Ringelberg, Eduardo Gross, Erik J. M. Koenen, Domingos Cardoso, George K. D. Ametsitsi, John Akomatey, Marta Maluk, Nisha Tak, Hukam S. Gehlot, Kathryn M. Wright, Neung Teaumroong, Pongpan Songwattana, Haroldo C. de Lima, Yves Prin, Charles E. Zartmann, Janet Sprent, Julie Ardley, Colin E. Hughes, Euan K. James
Summary: Nitrogen-fixing symbiosis is globally important, but the evolutionary history of nodulation is still debated. Recent evidence suggests a single origin of nodulation followed by massive parallel losses. Nodulation is mainly restricted to the subfamilies Papilionoideae and Caesalpinioideae within legumes, with the latter showing more stable retention of nodulation. In the subfamily Caesalpinioideae, two types of nodule anatomy were characterized: fixation thread nodules (FTs) and symbiosomes (SYMs). Using a phylogenomic tree, it was found that losses of nodulation are more common in lineages with FTs. The evolution of symbiosomes allows for a more intimate and enduring symbiosis, resulting in greater stability of nodulation in this species-rich clade of pantropical legumes.
Article
Environmental Sciences
Guaciara M. Santos, Rafael Perpetuo Albuquerque, Claudia Franca Barros, Santiago Ancapichun, Rose Oelkers, Laia Andreu-Hayles, Sergio Miana de Faria, Ricardo De Pol-Holz, Arno Fritz das Neves Brandes
Summary: This study presents a precisely measured C-14 dating sequence from 1938 to 2007 in tree rings of Hymenolobium petraeum, a tree species from the Central Brazilian Amazon. The results show that atmospheric C-14 records have been limited along tropical latitudes. The study also finds that the inter-hemispheric air-mass transport of excess-C-14 injected into the stratosphere during atmospheric nuclear tests is relatively uniform across distinct longitudinal regions. Additionally, the research reveals the significant influence of mining operations and shipping traffic on inland waterways in Porto Trombetas.
ENVIRONMENTAL RESEARCH
(2022)
Article
Chemistry, Medicinal
Ritesh Raju, Dhanushka Gunawardena, Paul Reddell, Gerald Munch
Summary: A phytochemical investigation of the leaves of the Australian rainforest tree Cryptocarya mackinnoniana led to the discovery of new oxygenated phenyl alkyl acids with weak to moderate anti-inflammatory activity.
Article
Biodiversity Conservation
Aaron J. Brunton, Robert W. Lamont, Gabriel C. Conroy, Samantha Yap, Maurizio Rossetto, Alyce Taylor-Brown, Laurent Maggia, Paul W. Reddell, Steven M. Ogbourne
Summary: Our study assessed the phylogeny of seven Fontainea taxa using chloroplast DNA sequence data and reduced-representation genome sequencing. We found three distinct lineages and several sister species. Importantly, we discovered that the vulnerable Fontainea australis and the critically endangered F. oraria form a sister subclade with evidence of shared plastid genotypes.
Article
Forestry
Gustavo Wyse Abaurre, Orivaldo Jose Saggin Junior, Sergio Miana de Faria
Summary: Inoculation with nitrogen-fixing bacteria and arbuscular mycorrhizal fungi significantly promotes the growth of Samanea saman seedlings, with Sewage Sludge substrate showing the best growth results.
FLORESTA E AMBIENTE
(2021)
