Review
Forestry
Dan Aoki, Yasuyuki Matsushita, Kazuhiko Fukushima
Summary: Imaging mass spectrometry is a method used to detect various phenomena in living physiological systems under hydrated conditions. However, the high vacuum conditions required for this method can lead to rapid drying of the sample surface, affecting cell survival. Combining freeze-fixation and a cryo sample stage is a promising approach to maintain the original situation of cells for mass spectrometry analysis.
Article
Plant Sciences
Qinyue Gong, Dan Aoki, Yasuyuki Matsushita, Masato Yoshida, Toru Taniguchi, Keita Endoh, Kazuhiko Fukushima
Summary: In this study, the distribution of eight alkaloids in freeze-fixed stems of Phellodendron amurense in fall and summer seasons was visualized, revealing variation in relative contents in different positions with seasons. High-performance liquid chromatography analysis further confirmed the seasonal variation of alkaloid contents. The results suggest the potential roles of these alkaloids in the physiological processes of the plant and its response to environmental changes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microscopy
M. F. Hayles, D. A. M. De Winter
Summary: The integration of cryo-techniques into FIB-SEM has allowed for the study of frozen, hydrated samples in Life Sciences. Cryo-TEM and cryo-SEM have provided valuable 2D information, while FIB-milling in cryo-FIB-SEM has added a third dimension for imaging purposes. This paper discusses the production of suitable cross-section results from Life Science samples using cryo-FIB-SEM.
JOURNAL OF MICROSCOPY
(2021)
Article
Biochemistry & Molecular Biology
Min Yu, Dan Aoki, Takuya Akita, Syunya Fujiyasu, Shunsuke Takada, Yasuyuki Matsushita, Masato Yoshida, Kazuhiko Fukushima
Summary: In this study, cryo-TOF-SIMS/SEM was used to investigate the distribution of lignans and lignan glucosides in Ginkgo biloba stems. The most abundant lignan glucoside, olivil DG, was found to be mainly distributed in the phloem, ray parenchyma cells, and pith of Ginkgo biloba. The comparative accumulation of olivil DG provided insights into its transport pathways and storage sites in Ginkgo biloba. These findings contribute to the understanding of the biological functions of lignans and lignan glucosides.
Article
Chemistry, Physical
Hye Hyun Yu, Youngseo Lee, Yun-Sik Nam, Man-Ho Kim, Kang-Bong Lee, Yeonhee Lee
Summary: In this study, physical measurements and chemical analysis were used to investigate the properties and chemical composition of fermented wheat and rice starch. The results showed that fermented starch had better antiseptic properties and smaller particle size.
Article
Forestry
Shohei Yamagishi, Kengo Shigetomi, Syunya Fujiyasu, Dan Aoki, Tetsuro Uno, Kazuhiko Fukushima, Yuzou Sano
Summary: This study investigated the presence of ethanol-extractable pit membrane incrustations (PMIs) in 40 angiosperms and found PMIs in 11 species. The researchers revealed that in dried samples, PMIs consist of an artifactual coating of lipids originated from the parenchyma. This highlights the importance for researchers to take precautions against self-coating by intrinsic chemicals when analyzing plant cell walls.
Article
Chemistry, Multidisciplinary
Petr Klimek, Rupert Wimmer, Peter Meinlschmidt
Summary: The cup-plant stalks were investigated as a potential wood-replacement material in particleboards, where differences in morphology were found to affect the resulting properties of the boards. While cup-plant boards performed slightly below spruce-made controls, three-layer cup-plant boards showed higher modulus of rupture and elasticity, along with lower thickness swelling, meeting the EN 312 standard P1 requirements for usage in dry conditions.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Jianguo Zhang, Danyang Zhang, Lei Sun, Gang Ji, Xiaojun Huang, Tongxin Niu, Jiashu Xu, Chengying Ma, Yun Zhu, Ning Gao, Wei Xu, Fei Sun
Summary: This study reports an integrated workflow, VHUT-cryo-FIB, for efficiently preparing frozen hydrated tissue lamella for cryo-electron tomography studies. The workflow was validated by obtaining in situ structures of ribosomes from spinach and liver samples.
JOURNAL OF STRUCTURAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Wil Gardner, David A. Winkler, Suzanne M. Cutts, Steven A. Torney, Geoffrey A. Pietersz, Benjamin W. Muir, Paul J. Pigram
Summary: Feature extraction algorithms are important for reducing data dimensionality, but most methods do not consider spatial information. This study introduces a convolutional autoencoder algorithm that can be applied to hyperspectral ToF-SIMS imaging and achieves better results.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Wil Gardner, David A. Winkler, Suzanne M. Cutts, Steven A. Torney, Geoffrey A. Pietersz, Benjamin W. Muir, Paul J. Pigram
Summary: Feature extraction algorithms are important for reducing data dimensionality, but most methods do not consider spatial information. This study introduces a convolutional autoencoder (CNNAE) specifically designed for hyperspectral ToF-SIMS imaging data, which incorporates pixel neighborhood information through convolutional layers. Compared to other methods, the CNNAE produces features with higher contrast and autocorrelation, accurately representing histologically recognizable features in the data. Additionally, the extension of CNNAE to 3D ToF-SIMS images provides a proof of principle for analyzing more complex 3D datasets.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Marcie B. Wiggins, Mengyu Liu, Catherine Matsen, Chang Liu, Karl S. Booksh
Summary: This study analyzed the painted ceiling decoration of Lin'xi Pavilion in the Forbidden City during the Ming and Qing dynasties, identifying similar organic and inorganic materials characteristic of both time periods, as well as a shift from natural mineral sources to synthetic copper-based pigments for these larger architectural projects.
Article
Forestry
Haruhiko Taneda, Kenich Yazaki, Tokiyoshi Hiramatsu, Bunnichi Shimizu, Daisuke Sugiura, Yoshiyuki Miyazawa
Summary: This study introduces a simple and effective method to distinguish between water- and air-filled conduits in wood by observing the xylem cross-section of frozen conifer stems. By using a digital camera to photograph the frozen stem cross-section, the distribution of water in the wood can be easily monitored, providing insight into the adaptive strategies of plants in extreme environments.
TREES-STRUCTURE AND FUNCTION
(2021)
Article
Materials Science, Multidisciplinary
Anna Dobkowska, Millicent Dayle H. Castillo, Joseph P. Turnbull, Sridhar Ramamurthy, Dmitrij Zagidulin, Desmond E. Moser, Mehran Behazin, Peter G. Keech, David W. Shoesmith, James J. Noel
Summary: The study focused on the corrosion behavior of coarse- and fine-grained copper materials in an aerated aqueous nitric acid solution. Crystallographic orientations greatly influenced the corrosion rate in coarse-grained materials, while grain boundary etching and grain boundary pores played a significant role in fine-grained materials.
Article
Nanoscience & Nanotechnology
Sofia Marchesini, Benjamen P. Reed, Helen Jones, Lidija Matjacic, Timothy E. Rosser, Yundong Zhou, Barry Brennan, Mariavitalia Tiddia, Rhodri Jervis, Melanie. J. Loveridge, Rinaldo Raccichini, Juyeon Park, Andrew J. Wain, Gareth Hinds, Ian S. Gilmore, Alexander G. Shard, Andrew J. Pollard
Summary: This article discusses the measurement challenges that affect surface analysis techniques used for Li-ion battery degradation studies and provides recommendations for improving reproducibility and reducing uncertainty. The article highlights key measurement issues that need to be addressed.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Nanoscience & Nanotechnology
Sofia Marchesini, Benjamen P. Reed, Helen Jones, Lidija Matjacic, Timothy E. Rosser, Yundong Zhou, Barry Brennan, Mariavitalia Tiddia, Rhodri Jervis, Melanie. J. Loveridge, Rinaldo Raccichini, Juyeon Park, Andrew J. Wain, Gareth Hinds, Ian S. Gilmore, Alexander G. Shard, Andrew J. Pollard
Summary: Understanding and mitigating the degradation mechanisms in lithium-ion batteries is crucial for improving their performance and lifespan. Surface analysis techniques are commonly employed to study the changes in chemistry and structure at reactive interfaces, but the complexity of battery electrodes and lack of standard measurement protocols can result in unreliable data. This paper discusses the measurement challenges of several key surface analysis techniques and provides recommendations to improve reproducibility and reduce uncertainty in the analysis of lithium-ion battery electrodes.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Polymer Science
Masaru Kajihara, Dan Aoki, Yasuyuki Matsushita, Kazuhiko Fukushima
JOURNAL OF APPLIED POLYMER SCIENCE
(2018)
Article
Multidisciplinary Sciences
Peiming Zheng, Dan Aoki, Masako Seki, Tsunehisa Miki, Soichi Tanaka, Kozo Kanayama, Yasuyuki Matsushita, Kazuhiko Fukushima
SCIENTIFIC REPORTS
(2018)
Article
Forestry
Yasuyuki Matsushita, Masaya Okayama, Dan Aoki, Sachie Yagami, Kazuhiko Fukushima
Article
Forestry
Yasuyuki Matsushita, Hiroki Endo, Shiho Takahashi, Dan Aoki, Kazuhiko Fukushima, Tatsuhiko Yamada
JOURNAL OF WOOD SCIENCE
(2018)
Article
Ecology
Hui Wang, Peiming Zheng, Dan Aoki, Takashi Miyake, Sachie Yagami, Yasuyuki Matsushita, Kazuhiko Fukushima, Michiko Nakagawa
ECOLOGY AND EVOLUTION
(2018)
Article
Forestry
Qiang Liu, Yasuyuki Matsushita, Dan Aoki, Kazuhiko Fukushima
JOURNAL OF WOOD SCIENCE
(2019)
Article
Forestry
Yasuyuki Matsushita, Daisuke Baba, Dan Aoki, Kazuhiko Fukushima
JOURNAL OF WOOD SCIENCE
(2019)
Article
Multidisciplinary Sciences
Taku Tsuyama, Yasuyuki Matsushita, Kazuhiko Fukushima, Keiji Takabe, Kazufumi Yazaki, Ichiro Kamei
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Yasuyuki Matsushita, Yuto Oyabu, Dan Aoki, Kazuhiko Fukushima
ROYAL SOCIETY OPEN SCIENCE
(2019)
Article
Agriculture, Multidisciplinary
Yasuyuki Matsushita, Sachie Yagami, Ayano Kato, Hayato Mitsuda, Dan Aoki, Kazuhiko Fukushima
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Plant Sciences
Hideto Hiraide, Yuki Tobimatsu, Arata Yoshinaga, Pui Ying Lam, Masaru Kobayashi, Yasuyuki Matsushita, Kazuhiko Fukushima, Keiji Takabe
Summary: This study demonstrates the crucial role of laccases in spatially organizing the polymerization of H-type and G-type monolignols during compression wood formation in Japanese cypress. The precise localization and differential oxidation activities of CoLac1 and CoLac3 towards H-type and G-type monolignols suggest that the spatial patterning of lignin polymerization in gymnosperm compression wood is dictated by the spatial localization and biochemical characteristics of laccases.
Review
Forestry
Dan Aoki, Yasuyuki Matsushita, Kazuhiko Fukushima
Summary: Imaging mass spectrometry is a method used to detect various phenomena in living physiological systems under hydrated conditions. However, the high vacuum conditions required for this method can lead to rapid drying of the sample surface, affecting cell survival. Combining freeze-fixation and a cryo sample stage is a promising approach to maintain the original situation of cells for mass spectrometry analysis.
Article
Biochemistry & Molecular Biology
Min Yu, Dan Aoki, Takuya Akita, Syunya Fujiyasu, Shunsuke Takada, Yasuyuki Matsushita, Masato Yoshida, Kazuhiko Fukushima
Summary: In this study, cryo-TOF-SIMS/SEM was used to investigate the distribution of lignans and lignan glucosides in Ginkgo biloba stems. The most abundant lignan glucoside, olivil DG, was found to be mainly distributed in the phloem, ray parenchyma cells, and pith of Ginkgo biloba. The comparative accumulation of olivil DG provided insights into its transport pathways and storage sites in Ginkgo biloba. These findings contribute to the understanding of the biological functions of lignans and lignan glucosides.
Article
Forestry
Sonoka Miyata, Dan Aoki, Yasuyuki Matsushita, Miyuki Takeuchi, Kazuhiko Fukushima
Summary: This study cultivated Ginkgo biloba L. saplings in a growth chamber with C-13 for two months. The C-13 ratio of the newly developed xylem region was evaluated using high lateral resolution secondary ion mass spectrometry and thioacidolysis/GC-MS. Solid-state C-13 direct polarisation/magic angle spinning (DP/MAS) NMR measurements were conducted for different wood samples, and the results suggested structural alterations of lignin during the sample preparation procedure, including ball milling.
Article
Chemistry, Multidisciplinary
Tobias Schmidt De Leon, Maria Laura Salum, Yasuyuki Matsushita, Kazuhiko Fukushima, Maria Eugenia Monge, Rosa Erra-Balsells
Summary: Cinnamic acid derivatives, specifically Z-sinapinic acid (ZSA), have been found to be more efficient in carbohydrate analysis in mass spectrometry. The formation of more stable complexes between ZSA and carbohydrates has been observed, providing insights into the matrix-analyte interaction model.
NEW JOURNAL OF CHEMISTRY
(2022)
