Article
Marine & Freshwater Biology
Chiara Fogliano, Chiara Maria Motta, Paola Venditti, Gianluca Fasciolo, Gaetana Napolitano, Bice Avallone, Rosa Carotenuto
Summary: Benzodiazepines, a major class of emerging water pollutants, have significant effects on the embryogenesis and molecular level of aquatic species, leading to physiological defects and developmental changes.
AQUATIC TOXICOLOGY
(2022)
Article
Plant Sciences
Shuai Lu, Cong Gao, Yongzhou Wang, Yingying He, Junrong Du, Moxian Chen, Hua Zhao, Hui Fang, Baohua Wang, Yunying Cao
Summary: This study identified U2AF65A in plant species, conducted phylogenetic and bioinformatic analyses, and studied the localization and stress responses of OsU2AF65A in rice. Using Arabidopsis and rice mutants, the importance of U2AF65A in plant growth and stress responses was demonstrated. These findings provide an overview of the U2AF65 gene family and its role in plant development and stress tolerance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Forestry
Liping Zhang, Shan Jin, Peixian Bai, Shibei Ge, Peng Yan, Zhengzhen Li, Lan Zhang, Wenyan Han, Jianming Zeng, Xin Li
Summary: This study systematically analyzed the CsYUC gene family in tea plants, identifying 17 CsYUC members and analyzing their physical and chemical properties, gene structures, and conserved domains. The study found that certain CsYUC genes may play key roles in seed development and the coordination of leaf bud and flower bud growth. The expression patterns of CsYUC genes under low temperature, drought, and NaCl stress were also investigated. Overall, this study provides a research basis for exploring the gene functions of the CsYUC family in tea plants.
Article
Multidisciplinary Sciences
Isao Matsui, Ayumi Matsumoto, Kazunori Inoue, Yusuke Katsuma, Seiichi Yasuda, Karin Shimada, Yusuke Sakaguchi, Masayuki Mizui, Jun-ya Kaimori, Yoshitsugu Takabatake, Yoshitaka Isaka
Summary: The coordinated growth and differentiation of multiple cells in kidney development were examined through re-analysis of single-cell RNA sequencing data, revealing lineage relationships and insights into differentiation processes. RNA velocity and NicheNet analyses provided insights into the self-renewal processes of nephron progenitors and the potential influence factors on cells involved in nephron development.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Alexandros Sountoulidis, Sergio Marco Salas, Emelie Braun, Christophe Avenel, Joseph Bergenstrahle, Jonas Theelke, Marco Vicari, Paulo Czarnewski, Andreas Liontos, Xesus Abalo, Zaneta Andrusivova, Reza Mirzazadeh, Michaela Asp, Xiaofei Li, Lijuan Hu, Sanem Sariyar, Anna Martinez Casals, Burcu Ayoglu, Alexandra Firsova, Jakob Michaelsson, Emma Lundberg, Carolina Waehlby, Erik Sundstroem, Sten Linnarsson, Joakim Lundeberg, Mats Nilsson, Christos Samakovlis
Summary: Sountoulidis et al. have created a spatial gene expression atlas of the human embryonic lung during the first trimester of gestation, identifying 83 cell states and developmental trajectories. They integrated single-cell RNA sequencing and spatially resolved transcriptomics, providing an open platform for interactive exploration. The atlas can serve as a reference for further research and understanding of pulmonary diseases.
NATURE CELL BIOLOGY
(2023)
Article
Biology
Katherine D. Chau, Mariam Shamekh, Jesse Huisken, Sandra M. Rehan
Summary: This study provides a detailed analysis of the influence of maternal care on gene expression and the microbiome in the development of wild bees. The results show that maternal care has the greatest impact on gene expression during early development, with a high enrichment of transcription factors regulating immune response. Additionally, it was found that fungi were the most abundant group in the microbiome, particularly Aspergillus in early larvae raised without mothers.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ting Zhao, Jingyun Lu, Huairen Zhang, Mande Xue, Jie Pan, Lijun Ma, Frederic Berger, Danhua Jiang
Summary: This study reveals the crucial role of Arabidopsis histone variant H3.3 in endowing seeds with post-embryonic developmental potentials and regulating germination and post-embryonic development. H3.3 facilitates chromatin opening at regulatory regions, regulates gene transcriptional regulation during germination, and is associated with gene body DNA methylation and the restriction of chromatin accessibility and cryptic transcription at specific gene ends in seeds.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Wellison J. S. Diniz, Matthew S. Crouse, Robert A. Cushman, Kyle J. McLean, Joel S. Caton, Carl R. Dahlen, Lawrence P. Reynolds, Alison K. Ward
Summary: This study investigated the impact of nutrient restriction on gene expression in fetal bovine cerebrum, liver, and muscle tissues, revealing factors that may negatively affect myogenesis. Nutrient restriction led to differential tissue regulation and affected gene expression changes in nutrient-sensing pathways. By identifying major regulators driving gene expression, further research is still needed to determine the impact of early maternal nutrition and strategic supplementation on pre- and post-natal performance.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Xu-Fei Du, Fu-Ning Li, Xiao-Lan Peng, Bing Xu, Yu Zhang, Guang Li, Taole Liu, Ying Li, Han Wang, Jun Yan, Jiu-Lin Du
Summary: By using in vivo long-term time-lapse imaging of retinotectal synapses in larval zebrafish, this study reveals that synaptogenesis exhibits circadian rhythm primarily through the formation rather than elimination of synapses, and it requires the hypocretinergic neural system's involvement. Disruption of this synaptogenic rhythm impacts the arrangement of retinotectal synapses and refinement of postsynaptic tectal neuron's receptive field. These findings demonstrate the hypocretin-dependent circadian regulation of developmental synaptogenesis, highlighting the important role of the circadian clock in neural development.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Deepak Adhikari, In-Won Lee, Usama Al-Zubaidi, Jun Liu, Qing-Hua Zhang, Wai Shan Yuen, Likun He, Yasmyn Winstanley, Hiromi Sesaki, Jeffrey R. Mann, Rebecca L. Robker, John Carroll
Summary: Disruption of mitochondrial function in oocytes through deletion of the mitochondrial fission factor Drp1 leads to changes in the oocyte transcriptome and proteome, resulting in altered establishment of the maternal epigenome and severe consequences for embryo development.
Article
Biochemistry & Molecular Biology
Julian O. Kimura, Marcela Bolanos, Lorenzo Ricci, Mansi Srivastava
Summary: This study reveals the cellular mechanism and molecular trajectory for the formation of adult pluripotent stem cells (aPSCs) during embryonic development. By utilizing lineage tracing and single-cell transcriptome profiling, the researchers identified a molecular trajectory for neoblast formation and showed that aPSCs derive from one lineage instead of multiple tissue-specific lineages.
Article
Agriculture, Dairy & Animal Science
Liyun Wang, Shaogang Li, Lele Xu, Yongqin Li, Huaxu Chen, Daohai Chen
Summary: This study conducted RNA-Seq analysis on the embryonic development of Sepiella japonica, resulting in the assembly of clean reads into unigenes and identification of differentially expressed genes and pathways. The study provided a fundamental genetic resource for further functional research.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Psychology, Clinical
Zahra M. Clayborne, Wendy Nilsen, Fartein Ask Torvik, Kristin Gustavson, Mona Bekkhus, Stephen E. Gilman, Golam M. Khandaker, Deshayne B. Fell, Ian Colman
Summary: This study examines the associations between prenatal maternal stress and child mental health outcomes, and the moderating effects of parenting behaviors. The results confirm the associations between prenatal maternal stress and children's mental health, and demonstrate that these associations may be modified by parenting behaviors.
PSYCHOLOGICAL MEDICINE
(2023)
Review
Nutrition & Dietetics
Maria Luisa Ojeda, Fatima Nogales, Ines Romero-Herrera, Olimpia Carreras
Summary: Nutrient intake of selenium by mothers during pregnancy and lactation can have a significant impact on infant tissue development. Low selenium supply may lead to oxidative stress affecting pregnancy and fetal development negatively, associated with metabolic imbalance and growth retardation in offspring.
Article
Public, Environmental & Occupational Health
Dana E. Goin, Monika A. Izano, Stephanie M. Eick, Amy M. Padula, Erin DeMicco, Tracey J. Woodruff, Rachel Morello-Frosch
Summary: This study found that food insecurity was most strongly associated with reduced birthweight for gestational age z-scores, while poor perceived neighborhood environment was associated with an increase in z-scores. The combination of food insecurity with unplanned pregnancy and stressful life events may have significant effects on fetal growth.
Article
Multidisciplinary Sciences
Woo Min Seo, Jiyoung Yoon, Ju-Hyun Lee, Yunjeong Lee, Hojae Lee, Dongho Geum, Woong Sun, Mi-Ryoung Song
Summary: Oxidative stress leads to axon degeneration and cell death, contributing to the development of neurodegenerative diseases. Researchers have successfully created motor nerve organoids from human pluripotent stem cells, providing a potential model for drug screening and evaluating axon regeneration.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Soo Hyun Yang, Esther Yang, Jaekwang Lee, Jin Yong Kim, Hyeijung Yoo, Hyung Sun Park, Jin Taek Jung, Dongmin Lee, Sungkun Chun, Yong Sang Jo, Gyeong Hee Pyeon, Jae-Yong Park, Hyun Woo Lee, Hyun Kim
Summary: Stress management is crucial for vertebrate survival. Chronic stress induces depression by activating GABAergic neurons in the lateral habenula (LHb), resulting in the inhibition of dopaminergic neurons in the ventral tegmental area (VTA). However, the impact of acute stress on the LHb-RMTg-VTA pathway remains elusive.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Hyoseon Oh, Suho Lee, Yusang Oh, Seongbin Kim, Young Seo Kim, Yeji Yang, Woochul Choi, Ye-Eun Yoo, Heejin Cho, Seungjoon Lee, Esther Yang, Wuhyun Koh, Woojin Won, Ryunhee Kim, C. Justin Lee, Hyun Kim, Hyojin Kang, Jin Young Kim, Taeyun Ku, Se-Bum Paik, Eunjoon Kim
Summary: This study reveals that the mutation of the ANK2 gene is associated with autism spectrum disorders (ASD). Knockout of the ANK2 gene in mice leads to ASD-related behavioral abnormalities and seizure-related death. The study further demonstrates that the ANK2 gene regulates neuronal excitability by modulating the length and density of Kv7 potassium channels in the axon initial segment (AIS), and Kv7 channelopathy is implicated in ANK2-related brain dysfunctions.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Kyubin Seo, Subin Cho, Hyogeun Shin, Aeri Shin, Ju-Hyun Lee, June Hoan Kim, Boram Lee, Hwanseok Jang, Youngju Kim, Hyo Min Cho, Yongdoo Park, Hee Youn Kim, Taeseob Lee, Woong-Yang Park, Yong Jun Kim, Esther Yang, Dongho Geum, Hyun Kim, Il-Joo Cho, Sanghyuk Lee, Jae Ryun Ryu, Woong Sun
Summary: This study successfully generated self-organized polarized spinal cord organoids using a geometrically confined culture system of human pluripotent stem cells. The proportions of dorsal/ventral domains in the organoids can be controlled by changes in the initial size of micropatterns. Mature organoids exhibit highly synchronized neural activity separately in the dorsal and ventral side, indicating functional and structural patterning.
Article
Multidisciplinary Sciences
Seunghwan Lee, Mingguang Cui, Donghun Lee, Kihoon Han, Woong Sun, Dongmin Lee
Summary: Accurate spatiotemporal control of multicellular self-organization is crucial in developmental stages, and Wnt signaling plays a major role in determining the anteroposterior axis of the embryo. Researchers developed a genetically encoded optochemogenetic Wnt switch, named optochemoWnt, which successfully modulated Wnt signaling with AND-gated patterns and improved signal-to-noise ratio (SNR). The optochemoWnt switch provides a safeguard against signal leakage and expands the molecular toolbox for developmental biology and tissue engineering.
Article
Multidisciplinary Sciences
Jisoo Lee, Soohyun Kim, Boram Lee, Yoo-Bin Kim, Kwang Hwan Kim, Gehoon Chung, Sung Joong Lee, Soojin Lee, Woong Sun, Hee-Kyung Park, Se-Young Choi
Summary: Salivary gland cells secrete water in response to neuronal stimulation and are closely connected to other neurons. These cells also express proteins responsible for neuronal function, but their exact role in salivary glands is largely unknown. In this study, the function of Neuronal growth regulator 1 (NEGR1) in salivary gland cells was investigated. NEGR1 was found to be expressed in both mouse and human salivary glands. Knockout of Negr1 gene in mice had no impact on the structure of salivary glands, but it did affect intracellular calcium levels and salivary secretion. NEGR1 was found to influence salivary secretion through muscarinic calcium signaling.
Article
Multidisciplinary Sciences
Byung Geun Ha, Yu-Jin Jang, EunSoo Lee, Byung-Gyu Kim, Kyungjae Myung, Woong Sun, Sung-Jin Jeong
Summary: The extracellular matrix (ECM) components play a role in maintaining cellular architecture and tissue morphology. Brain tissue, with its high cell density and weak mechanical strength, poses challenges in decellularization. We successfully obtained ECM components from mouse brains using a combination of decellularization and polymerization methods, which can be useful for obtaining matrisomal data and conducting functional studies.
Review
Engineering, Biomedical
Poojitha Pinjala, Kamatham Pushpa Tryphena, Renuka Prasad, Dharmendra Kumar Khatri, Woong Sun, Shashi Bala Singh, Dalapathi Gugulothu, Saurabh Srivastava, Lalitkumar Vora
Summary: Since its discovery in 2012, CRISPR Cas9 has been used as a direct treatment approach for correcting gene mutations and establishing animal models in neurodegenerative disorders like Parkinson's disease (PD). Stem cell biology has improved over the years, and scientists have developed personalized cell therapy using CRISPR/Cas9 to edit embryonic and patient-derived stem cells. This review emphasizes the importance of CRISPR/Cas9-based stem cell therapy in PD, both for developing disease models and exploring therapeutic strategies.
BIOMATERIALS RESEARCH
(2023)
Correction
Biochemistry & Molecular Biology
Heejin Cho, Taesun Yoo, Heera Moon, Hyojin Kang, Yeji Yang, MinSoung Kang, Esther Yang, Dowoon Lee, Hyun Kim, Daehee Hwang, Doyoun Kim, Jin Young Kim, Eunjoon Kim
MOLECULAR PSYCHIATRY
(2023)
Article
Biochemistry & Molecular Biology
Heejin Cho, Taesun Yoo, Heera Moon, Hyojin Kang, Yeji Yang, MinSoung Kang, Esther Yang, Dowoon Lee, Daehee Hwang, Hyun Kim, Doyoun Kim, Jin Young Kim, Eunjoon Kim
Summary: ADNP syndrome, characterized by developmental delay, intellectual disability, and autism spectrum disorders (ASD), involves the ADNP transcription factor of the SWI/SNF chromatin-remodeling complex. Adnp-haploinsufficient (Adnp-HT) mice display synaptic plasticity deficits associated with cognitive inflexibility and CaMKIIa hyperactivity. These mice show impaired contextual learning and memory, social deficits, and hyperphosphorylated CaMKIIa and its substrates in the adult hippocampus, which can be normalized by CaMKIIa inhibition.
MOLECULAR PSYCHIATRY
(2023)
Article
Multidisciplinary Sciences
Uikyu Chae, Jiwan Woo, Yakdol Cho, Jeong- Kyu Han, Soo Hyun Yang, Esther Yang, Hyogeun Shin, Hyun Kim, Hyun- Yong Yu, C. Justin Lee, Il-Joo Cho
Summary: Real-time monitoring of neurochemicals and electrical activity in the brain is essential for investigating neural circuits. We have developed a real-time bimodal neural probe that can simultaneously measure multiple neurochemicals without cross-talk and record electrical activity. Using this probe, we have demonstrated simultaneous measurements of glucose, lactate, choline, and glutamate, as well as functional connectivity between brain regions. This device has the potential to greatly enhance our understanding of neurochemicals in neural circuits and aid in the development of drugs for brain diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell & Tissue Engineering
Ju-Hyun Lee, Mohammed R. Shaker, Si-Hyung Park, Woong Sun
Summary: In vertebrates, the formation of the neural tube during neurulation is crucial for the development of the central nervous system. However, the specific molecular pathways leading to neural tube defects (NTDs) are not well understood. Through the use of human spinal cord organoids (hSCOs), it was discovered that valproic acid (VPA) can cause defects in neurulation. Further analysis revealed significant changes in cell-cell junctional genes/proteins in VPA-treated organoids, and similar gene expression abnormalities and NTD phenotypes were observed in VPA-treated mouse embryos. This study highlights the importance of hSCOs as a valuable resource for studying the molecular mechanisms of human neurulation.
INTERNATIONAL JOURNAL OF STEM CELLS
(2023)
Article
Chemistry, Multidisciplinary
Jungryun Kim, Paramesh Jangili, Jeongah Kim, Stephani Edwina Lucia, Jae Ryun Ryu, Renuka Prasad, Soyu Zi, Pilhan Kim, Woong Sun, Jong Seung Kim
Summary: Despite the availability of many fluorescent probes targeting mitochondria, there is still a need for the development of low-cytotoxic probes to improve the accuracy of mitochondrial function assessment. In this study, a novel cyanine-based NIR fluorescent probe, T2, was developed, which selectively targets mitochondria and has significantly low toxicity by modulating the intracellular redox status. Furthermore, T2 inhibits oxidative stress-induced cell death in cortical neurons. This study provides new insights into the development of low-toxic mitochondrial imaging agents by regulating redox homeostasis.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Mingguang Cui, Seunghwan Lee, Sung Hwan Ban, Jae Ryun Ryu, Meiying Shen, Soo Hyun Yang, Jin Young Kim, Seul Ki Choi, Jaemin Han, Yoonhee Kim, Kihoon Han, Donghun Lee, Woong Sun, Hyung-Bae Kwon, Dongmin Lee
Summary: Proteases, particularly potyviral protease, have been employed as molecular switches in synthetic biological circuits. LAUNCHER, a single-component switch utilizing circularly permutated tobacco etch virus protease and blue-light-gated substrate, offers superior signal-to-noise ratio and precise payload release, making it suitable for diverse cellular applications and enhanced circuit performance.
NATURE CHEMICAL BIOLOGY
(2023)
