Article
Behavioral Sciences
Douglas F. Covey, Alex S. Evers, Yukitoshi Izumi, Jamie L. Maguire, Steven J. Mennerick, Charles F. Zorumski
Summary: Endogenous neurosteroids and synthetic neuroactive steroids are significant targets for therapeutic development in neuropsychiatric disorders. Studies on unnatural enantiomers of key neurosteroids have provided insights into the interactions of synthetic steroids with receptors, ion channels, and intracellular sites of action. The effects of these enantiomers suggest that they may have therapeutic potential that complements or exceeds their natural counterparts.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2023)
Article
Clinical Neurology
Cecilia Rustichelli, Elisa Bellei, Stefania Bergamini, Emanuela Monari, Flavia Lo Castro, Carlo Baraldi, Aldo Tomasi, Anna Ferrari
Summary: This study found that serum levels of pregnenolone sulfate and pregnanolone were significantly lower in women with menstrually-related migraine compared to controls, while estradiol levels were similar. In the migraine group, serum levels of pregnenolone sulfate were negatively correlated with migraine history and age, while in the control group, pregnenolone sulfate levels were not age-related. These findings suggest a potential role of neurosteroids in migraine pathophysiology and support the therapeutic potential of restoring low neurosteroid levels in migraine treatment.
JOURNAL OF HEADACHE AND PAIN
(2021)
Article
Multidisciplinary Sciences
Chao-Hua Jiang, Mengping Wei, Chen Zhang, Yun Stone Shi
Summary: Long-term potentiation (LTP) is a crucial cellular mechanism for learning and memory, with the amino-terminal domain (ATD) of GluA1 interacting with neuroplastin-65 (Np65) playing a key role in anchoring AMPARs at the postsynaptic membrane during LTP.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Forrest J. Ragozzino, Rachel A. Arnold, Axel J. Fenwick, Timothy P. Riley, Jonathan E. M. Lindberg, BreeAnne Peterson, James H. Peters
Summary: TRPM3 plays a significant role in controlling spontaneous glutamate release in vagal afferents and serves as an additional temperature-sensitive calcium conductance. Its genetic deletion does not affect synchronous or asynchronous glutamate release, indicating its specific role in regulating temperature-driven neurotransmission in autonomic reflex function.
JOURNAL OF NEUROPHYSIOLOGY
(2021)
Article
Clinical Neurology
G. Krivoshein, E. A. Tolner, A. M. J. M. van den Maagdenberg, R. A. Giniatullin
Summary: This study found that agonists of the TRPM3 channels induced nociceptive firing in mouse meninges, with a more significant response in female mice. However, no sex differences were observed for agonists of the Piezo1 channels and TRPV1 channels. These findings suggest a potential role for TRPM3 channels in the generation of migraine pain in females.
JOURNAL OF HEADACHE AND PAIN
(2022)
Article
Microbiology
Lina Yao, Gabriel D. D'Agostino, Jinseok Park, Saiyu Hang, Arijit A. Adhikari, Yancong Zhang, Wei Li, Julian Avila-Pacheco, Sena Bae, Clary B. Clish, Eric A. Franzosa, Curtis Huttenhower, Jun R. Huh, A. Sloan Devlin
Summary: This study characterizes a biosynthetic pathway in gut bacteria that sulfonates steroidal metabolites, potentially impacting immune cell trafficking and inflammatory bowel disease.
NATURE MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Dagimhiwat H. Legesse, Chen Fan, Jinfeng Teng, Yuxuan Zhuang, Rebecca J. Howard, Colleen M. Noviello, Erik Lindahl, Ryan E. Hibbs
Summary: Legesse et al. present structural studies of a human GABAA receptor in complex with positive and negative modulator neurosteroids, uncovering mechanisms of potentiation and inhibition. Allopregnanolone, a positive modulator, binds at the receptor-bilayer interface, while inhibitory neurosteroids bind in the pore. MD simulations and electrophysiology support the mechanism of allopregnanolone potentiating channel activity and suggest pore block as the dominant mechanism for sulfated neurosteroid inhibition.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Yuewen Ding, Zheye Zhou, Jinyu Chen, Yu Peng, Haitao Wang, Wei Qiu, Wei Xie, Jun Zhang, Honghao Wang
Summary: The study established a novel mouse model of anti-NMDAR encephalitis by actively immunizing against NMDARs with a peptide targeting the ATD of GluN1. Immunized mice showed significant memory loss and decreased surface NMDAR cluster density in hippocampal neurons, similar to effects observed in patients with anti-NMDAR encephalitis. These findings may contribute to further research on the pathogenesis of anti-NMDAR encephalitis and the development of new therapies for this disease.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Pharmacology & Pharmacy
Md Zubayer Hossain Saad, Liuruimin Xiang, Yan-Shin Liao, Leah R. Reznikov, Jianyang Du
Summary: The study identified molecular determinants responsible for the pH regulation of TRPM3, showing that both intracellular and extracellular protons block TRPM3 through binding sites in the pore region. Specific residue D1073 was found to be essential for pH sensitivity in TRPM3 channel.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Plant Sciences
Justyna Piotrowska, Yuki Jodoi, Nguyen Ha Trang, Anna Wawrzynska, Hideki Takahashi, Agnieszka Sirko, Akiko Maruyama-Nakashita
Summary: The C-terminal region of the SLIM1 transcription factor plays a crucial role in gene expression regulation and is similar to the regulation of the ethylene signaling pathway. Experimental results demonstrate that the C-terminal region is essential for the transcription activation and functional restoration of SLIM1.
Article
Biochemistry & Molecular Biology
Subhra Kanti Das, Ashok Kumar, Fanfan Hao, Amber R. Cutter Dipiazza, He Fang, Tae-Hee Lee, Jeffrey J. Hayes
Summary: The H1 C-terminal domain (CTD) undergoes distinct static and dynamic structural changes upon binding to nucleosomes, which can be decondensed through acetylation. The interactions between H1 CTD and linker DNA primarily occur between the first 30 residues of CTD and the first -25 bp of linker DNA.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Kedzia, Lukasz Dobrzycki, Marcin Wilczek, Karolina Pulka-Ziach
Summary: In this study, hybrids of short oligourea foldamers with residues of different amino acids esters were synthesized and reacted with LiOH. Ester hydrolysis and cyclization reaction were observed as possible transformations under these conditions. The reaction was investigated using different C-terminal residue structures, as well as under different work-up conditions. The stability of the resulting products, including hydantoin rings, was also tested.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Endocrinology & Metabolism
Takayoshi Ubuka, Kazuyoshi Tsutsui
Summary: Neurosteroids and neuropeptides play important pleiotropic roles in the central nervous system, potentially regulating the biosynthesis of neurosteroids by controlling enzyme activities. Investigating this regulatory mechanism is crucial for understanding the physiological significance of this new neuroendocrinological phenomenon.
FRONTIERS IN NEUROENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Roa'a Jaradat, Xiaole Li, Honghong Chen, Peter B. Stathopulos, Donglin Bai
Summary: Functional studies and structural models of Cx46 and Cx50 GJs reveal the importance of hydrophobic interactions between the NT and TM2 domains in V-j-gating, gamma(j), and channel open stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Goro Kato
Summary: This review discusses the structural and functional aspects of Src protein and its regulatory mechanism. By reviewing nuclear magnetic resonance analyses and recent studies, the authors explore new characteristics and regulatory roles of Src protein. Finally, the new regulatory roles are integrated with the canonical model to elucidate the functions of full-length Src.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Nicole A. Doria-Rose, Jinal N. Bhiman, Ryan S. Roark, Chaim A. Schramm, Jason Gorman, Gwo-Yu Chuang, Marie Pancera, Evan M. Cale, Michael J. Ernandes, Mark K. Louder, Mangaiarkarasi Asokan, Robert T. Bailer, Aliaksandr Druz, Isabella R. Fraschilla, Nigel J. Garrett, Marissa Jarosinski, Rebecca M. Lynch, Krisha McKee, Sijy O'Dell, Amarendra Pegu, Stephen D. Schmidt, Ryan P. Staupe, Matthew S. Sutton, Keyun Wang, Constantinos Kurt Wibmer, Barton F. Haynes, Salim Abdool-Karim, Lawrence Shapiro, Peter D. Kwong, Penny L. Moore, Lynn Morris, John R. Mascola
JOURNAL OF VIROLOGY
(2016)
Article
Microbiology
Nicole A. Doria-Rose, Han R. Altae-Tran, Ryan S. Roark, Stephen D. Schmidt, Matthew S. Sutton, Mark K. Louder, Gwo-Yu Chuang, Robert T. Bailer, Valerie Cortez, Rui Kong, Krisha McKee, Sijy O'Dell, Felicia Wang, Salim S. Abdool Karim, James M. Binley, Mark Connors, Barton F. Haynes, Malcolm A. Martin, David C. Montefiori, Lynn Morris, Julie Overbaugh, Peter D. Kwong, John R. Mascola, Ivelin S. Georgiev
Article
Multidisciplinary Sciences
Nicole A. Doria-Rose, Chaim A. Schramm, Jason Gorman, Penny L. Moore, Jinal N. Bhiman, Brandon J. DeKosky, Michael J. Ernandes, Ivelin S. Georgiev, Helen J. Kim, Marie Pancera, Ryan P. Staupe, Han R. Altae-Tran, Robert T. Bailer, Ema T. Crooks, Albert Cupo, Aliaksandr Druz, Nigel J. Garrett, Kam H. Hoi, Rui Kong, Mark K. Louder, Nancy S. Longo, Krisha McKee, Molati Nonyane, Sijy O'Dell, Ryan S. Roark, Rebecca S. Rudicell, Stephen D. Schmidt, Daniel J. Sheward, Cinque Soto, Constantinos Kurt Wibmer, Yongping Yang, Zhenhai Zhang, James C. Mullikin, James M. Binley, Rogier W. Sanders, Ian A. Wilson, John P. Moore, Andrew B. Ward, George Georgiou, Carolyn Williamson, Salim S. Abdool Karim, Lynn Morris, Peter D. Kwong, Lawrence Shapiro, John R. Mascola
Article
Multidisciplinary Sciences
Joel A. Butterwick, Josefina del Marmol, Kelly H. Kim, Martha A. Kahlson, Jackson A. Rogow, Thomas Walz, Vanessa Ruta
Article
Multidisciplinary Sciences
Ryan S. Roark, Hui Li, Wilton B. Williams, Hema Chug, Rosemarie D. Mason, Jason Gorman, Shuyi Wang, Fang-Hua Lee, Juliette Rando, Mattia Bonsignori, Kwan-Ki Hwang, Kevin O. Saunders, Kevin Wiehe, M. Anthony Moody, Peter T. Hraber, Kshitij Wagh, Elena E. Giorgi, Ronnie M. Russell, Frederic Bibollet-Ruche, Weimin Liu, Jesse Connell, Andrew G. Smith, Julia DeVoto, Alexander Murphy, Jessica Smith, Wenge Ding, Chengyan Zhao, Neha Chohan, Maho Okumura, Christina Rosario, Yu Ding, Emily Lindemuth, Anya M. Bauer, Katharine J. Bar, David Ambrozak, Cara W. Chao, Gwo-Yu Chuang, Hui Geng, Bob C. Lin, Mark K. Louder, Richard Nguyen, Baoshan Zhang, Mark G. Lewis, Donald D. Raymond, Nicole A. Doria-Rose, Chaim A. Schramm, Daniel C. Douek, Mario Roederer, Thomas B. Kepler, Garnett Kelsoe, John R. Mascola, Peter D. Kwong, Bette T. Korber, Stephen C. Harrison, Barton F. Haynes, Beatrice H. Hahn, George M. Shaw
Summary: Research has shown that in rhesus macaques, primary HIV-1 envelope proteins can trigger patterns of coevolution with antibodies similar to those observed in humans, providing precise solutions for virus persistence. These findings can guide the design of HIV-1 vaccines by recapitulating the developmental features of human broadly neutralizing antibodies.
Article
Neurosciences
Martha A. Kahlson, Kenneth J. Colodner
JOURNAL OF EXPERIMENTAL NEUROSCIENCE
(2015)
Article
Biochemistry & Molecular Biology
Olexander M. Semenenko, Victoria V. Lipson, Alina O. Sadchenko, Olga V. Vashchenko, Natalia A. Kasian, Liliia Sviechnikova, Longin M. Lisetski, Mykola L. Babak, Volodymyr M. Vakula, Oleksandr Borysov, Yuliia Holota, Sergey O. Zozulya, Petro O. Borysko, Olexander Mazepa
Summary: An efficient protocol for the synthesis of novel methotrexate-betulonic acid hybrids has been developed and their structures and properties have been fully established. The hybrids showed inferior interactions with artificial lipid membranes, possibly due to their increasing lipophilicity.
Article
Biochemistry & Molecular Biology
Mohadese Yaghoobi Anzabi, Piotr Cmoch, Roman Luboradzki, Zbigniew Pakulski
Summary: Friedelin and 3-acetoxyfriedel-3-en-2-one, known as friedelane triterpenoids, have been isolated from cork smoker wash solids and used as starting materials for the synthesis of new friedelane derivatives. Reduction reactions result in the formation of friedelinol and epi-cerin derivatives.
Article
Biochemistry & Molecular Biology
Detlef Thieme, Aniko Krumbholz, Martin Bidlingmaier, Christoph Geffert, Annika Hameder, Andreas Stoever, Matthias Graw, Annekathrin M. Keiler
Summary: Ethanol can affect testosterone metabolism and potentially influence steroid biosynthesis and blood steroid profiles. The study emphasizes the importance of sufficient time interval from food and ethanol intake during blood sampling, particularly for diagnostics in doping control.
Article
Biochemistry & Molecular Biology
Masanobu Murao, Tetsuo Imano, Yoshinobu Sato, Masaaki Nakajima
Article
Biochemistry & Molecular Biology
Jingyuan Wang, Ye Feng, Brian Liu, Wen Xie
Summary: Sulfation and desulfation of steroids are opposing processes that regulate steroid homeostasis and are involved in steroid-related diseases. Cytosolic sulfotransferase and steroid sulfatase modify and regulate steroids to affect hormonal dysregulation, metabolic diseases, and cancer.
Article
Biochemistry & Molecular Biology
Noah K. Babel, Brian J. Feldman
Summary: Our research identified a previously unrecognized ADAMTS1-PTN-Wnt pathway in regulating adipose precursor cell behavior. The non-muscle myosin protein MYH9 was found to be a key target of this pathway for modulating adipogenesis. These findings offer potential for developing novel therapeutics for obesity and related metabolic disorders.
Article
Biochemistry & Molecular Biology
Martiniano Bello
Summary: This study explores the structural and thermodynamic basis of molecular recognition between ERβ and DPN and its derivatives using molecular dynamics simulations and end-point methods. The results show that the MMGBSA approach is able to reproduce experimental trends and identify eight residues that strongly interact with the ligands.