Article
Cell Biology
Anastasiya A. Gorkun, Darla P. Revokatova, Irina M. Zurina, Denis A. Nikishin, Polina Y. Bikmulina, Peter S. Timashev, Anastasiya Shpichka, Nastasia Kosheleva, Tamara D. Kolokoltsova, Irina N. Saburina
Summary: The study suggests that arranging ADSCs into spheroids allows for rapid acquisition of markers for both osteogenesis and angiogenesis compared to 2D culture. This multidirectional differentiation persists over time, unaffected by classic protocols for osteogenic or angiogenic differentiation, while maintaining similar morphology and microarchitecture under different culture conditions. These findings provide insights into the regulation of differentiation processes and communication in multicellular structures, and have implications for tissue engineering and regeneration applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Sujee Jeyapalina, Elaine Hillas, James Peter Beck, Jayant Agarwal, Jill Shea
Summary: This study aimed to investigate the potential benefits of FA and FHA scaffolds in conjunction with ADSCs for bone regeneration. The results showed that FA and FHA surfaces could drive ADSCs towards osteogenic differentiation earlier, potentially improving bone regeneration following injury.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Angela Oranger, Roberta Zerlotin, Cinzia Buccoliero, Lorenzo Sanesi, Giuseppina Storlino, Ernestina Schipani, Kenneth Michael Kozloff, Giorgio Mori, Graziana Colaianni, Silvia Colucci, Maria Grano
Summary: Bone fractures are a common clinical event caused by falls, trauma, diseases, and aging. Recent research has found a link between irisin and bone fractures. In an experiment on mice with tibial fractures, treatment with r-Irisin reduced inflammation and promoted angiogenesis, cartilage matrix degradation, and bone formation, suggesting it as a potential molecule for fracture treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Article
Biochemistry & Molecular Biology
Elle Koivunotko, Jasmi Snirvi, Arto Merivaara, Riina Harjumaki, Swarna Rautiainen, Minna Kelloniemi, Kirsi Kuismanen, Susanna Miettinen, Marjo Yliperttula, Raili Koivuniemi
Summary: Adipose-derived mesenchymal stromal cells (ASCs) hold great potential for cellular therapies and can differentiate into endothelial cells (ECs) and other angiogenic cell types. Nanofibrillated cellulose (NFC) hydrogel is a suitable matrix for 3D cell culture of ASCs and can stimulate angiogenesis. This study recommends the use of NFC hydrogel as an animal-free biomaterial scaffold for therapeutic angiogenesis and other cell therapy purposes.
Article
Cell Biology
Bo Jia, Jun Chen, Qin Wang, Xiang Sun, Jiusong Han, Fernando Guastaldi, Shijian Xiang, Qingsong Ye, Yan He
Summary: The study reveals that SIRT6 physically interacts with DNMT1 protein, deacetylating and destabilizing it, leading to the activation of NOTCH1 and NOTCH2, which promotes the osteogenic differentiation of ADSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Baojun Sun, Yajie Sun, Shuwei Han, Ruitong Zhang, Xiujuan Wang, Chunxia Meng, Tuo Ji, Chunhui Sun, Na Ren, Shaohua Ge, Hong Liu, Yang Yu, Jingang Wang
Summary: Traditional bone defect treatments face limitations due to insufficient supply of autologous bone, immune rejection of allogeneic bone grafts, and high medical costs. To overcome these limitations, bone tissue engineering with electroactive scaffolds has emerged as a promising option. However, current electroactive scaffolds are either single-function and not suitable for scientific research and clinical application, or complex and expensive to prepare. This study proposes a new tissue engineering strategy to optimize the electrical properties and biocompatibility of carbon-based materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Multidisciplinary
Jong Bo Park, Ji Yeon Ahn, Woo Sub Yang, Seong Chae Park, Young Jin Jung, Myungook Lee, Byung Hee Hong, Jeong Mook Lim
Summary: This study investigated the effect of nanoscale spacing by stacked graphene film on osteogenic differentiation of human adipose-derived mesenchymal stromal cells. The results showed that osteogenic differentiation was promoted as the stacking layer increased, with the most significant effects observed in the three layers. The study demonstrated that multilayer-stacked graphene created nanoscale spaces promote calcium deposit and cytoskeletal integrity in hADSC-related, in vitro-osteogenesis.
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Cell Biology
Sheng Zhang, Yuanlong Xie, Feifei Yan, Yufeng Zhang, Zhiqiang Yang, Zhe Chen, Yong Zhao, Zan Huang, Lin Cai, Zhouming Deng
Summary: The study reveals that negative pressure wound therapy promotes bone regeneration and induces osteoblast differentiation in vitro. Negative pressure induces osteogenesis through activating large autophagy by AMPK-ULK1 signaling, which plays a crucial role in bone regeneration.
Article
Cell Biology
Yutong Wu, Hongbo Ai, Qiankun Yang, Ce Dou, Jianzhong Xu
Summary: In this study, it was found that osteoclast-derived small extracellular vesicles (OC-sEVs) inhibited Fam134a through miR-106a-5p and significantly promoted bone mesenchymal stem cell (BMSC) osteogenic mineralization in vitro. Additionally, demineralized bone matrix (DBM) scaffolds modified with miR-106a-5p-sEVs showed enhanced capacity for bone regeneration in a calvarial defect mouse model. This important finding emphasizes the critical role of sEV-mediated miR-106a-5p transfer in osteogenesis and reveals a novel communication mode between osteoclasts and BMSCs.
CELLULAR SIGNALLING
(2023)
Article
Biochemistry & Molecular Biology
Bing Sun, Rongmei Qu, Tingyu Fan, Yuchao Yang, Xin Jiang, Asmat Ullah Khan, Zhitao Zhou, Jingliao Zhang, Kuanhai Wei, Jun Ouyang, Jingxing Dai
Summary: This study suggests that the actin polymerization state may promote osteogenic differentiation of hASCs by regulating the protein expression of focal adhesion-associated proteins in a concentration-dependent manner. The findings provide valuable information for exploring the mechanism of osteogenic differentiation in hASCs.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2021)
Article
Engineering, Biomedical
Zhifa Wang, Leng Han, Tianyu Sun, Weijian Wang, Xiao Li, Buling Wu
Summary: Cell sheet techniques are widely used in bone engineering, and this study successfully induced bidiretionally differentiated ADSCs to form ADSC sheet-EPC complexes for bone regeneration. The complexes demonstrated dense and well-vascularized new bone tissue, showing promising results for repairing bone defects.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2021)
Article
Biotechnology & Applied Microbiology
Daowen Luo, Shuanglin Peng, Qing Li, Pengcheng Rao, Gang Tao, Lang Wang, Jingang Xiao
Summary: This study found that the expression of METTL3 decreased in adipose-derived stem cells (ASCs) from osteoporotic rats (OP-ASCs), leading to a decrease in their osteogenic ability. Overexpression of METTL3 could restore the osteogenic capacity of OP-ASCs, providing a new target for treating osteoporotic bone defects.
JOURNAL OF GENE MEDICINE
(2023)
Article
Cell & Tissue Engineering
Wentian Sun, Yidan Song, Kai Xia, Liyuan Yu, Xinqi Huang, Zhihe Zhao, Jun Liu
Summary: A transcriptome-wide m(6)A methylome was built during the osteogenic differentiation of human adipose-derived stem cells (hASCs), revealing the influence of m(6)A on various signaling pathways and hub genes related to bone regeneration. Further epigenetic studies based on these findings could enhance the understanding of the mechanisms underlying hASCs osteogenic differentiation.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Orthopedics
Yiwei Chen, Yu Miao, Kexin Liu, Feng Xue, Bin Zhu, Changqing Zhang, Guangyi Li
Summary: ONFH is a recalcitrant ischemic disorder classified into traumatic and nontraumatic categories. Histopathological and microstructural alterations lead to bone tissue necrosis and joint collapse. Imaging classification systems may underestimate disease severity.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Article
Orthopedics
Yiyang Ma, Kaiwen Zheng, Yidan Pang, Fuzhou Xiang, Junjie Gao, Changqing Zhang, Dajiang Du
Summary: This study demonstrates that synovium-derived stromal cells (SDSCs) can help coastal chondrocytes (CCs) maintain their chondrogenic phenotype and suppress hypertrophic differentiation in cartilage repairs through both direct and indirect coculture experiments. The presence of SDSCs in osteochondral defects promotes good restoration effects and lower expression levels of type X collagen, suggesting a potential role for SDSCs in cartilage repair.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Article
Orthopedics
Lenian Zhou, Hongyi Zhu, Shanyi Lin, Hanqiang Jin, Zhaoyuan Zhang, Yang Dong, Qingcheng Yang, Changqing Zhang, Ting Yuan
Summary: Extended curettage has become the preferred treatment for GCTB, but the high recurrence rate poses a challenge. CT is valuable in evaluating GCTB around the knee. Age, DTA (<2mm), and DPC were significant factors for recurrence prediction after extended curettage in a three-factor model.
INTERNATIONAL ORTHOPAEDICS
(2022)
Review
Biotechnology & Applied Microbiology
Haoyu Fang, Daoyu Zhu, Qianhao Yang, Yixuan Chen, Changqing Zhang, Junjie Gao, Youshui Gao
Summary: Bones are highly sophisticated tissues with remarkable potential for regeneration. Novel biomaterials designed to mimic bone microenvironment have significantly accelerated bone healing. These biomaterials are categorized as zero-dimensional to four-dimensional based on their dimensional structure and functional mechanism.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Geriatrics & Gerontology
Jinhui Zhao, Qianying Cai, Dajun Jiang, Lingtian Wang, Haiyan He, Shengbao Chen, Weitao Jia, Changqing Zhang
Summary: During the COVID-19 pandemic, elderly patients with hip fractures had a poorer prognosis despite testing negative for COVID-19. Factors such as timely admission, postoperative follow-up, and rehabilitation were found to significantly improve the prognosis of elderly COVID-19 negative patients with hip fractures.
CLINICAL INTERVENTIONS IN AGING
(2022)
Article
Pediatrics
Yiyang Ma, Hao Peng, Fuchou Hsiang, Haoyu Fang, Dajiang Du, Chenyi Jiang, Yehui Wang, Chun Chen, Changqing Zhang, Yun Gao
Summary: This study presents the clinical manifestations and diagnostic results of a patient with MPS IVA, revealing compound heterozygous variants of the GALNS gene. Additionally, it is the first study to describe the microarchitectural and histological changes of both subchondral bone and articular cartilage in the femoral head.
FRONTIERS IN PEDIATRICS
(2022)
Article
Chemistry, Applied
Danya Chen, Pei Liu, Mengna Li, Changqing Zhang, Youshui Gao, Yaping Guo
Summary: Nacre-mimetic scaffolds with layered structure and incorporated SP peptides were fabricated, showing excellent mechanical strength and biocompatibility, promoting MSC proliferation and differentiation, and enhancing subchondral bone regeneration in vivo.
CARBOHYDRATE POLYMERS
(2022)
Letter
Orthopedics
Lenian Zhou, Hongyi Zhu, Changqing Zhang, Ting Yuan
INTERNATIONAL ORTHOPAEDICS
(2022)
Article
Engineering, Biomedical
Yu-Wei Ge, Zhang-Hao Fan, Qin-Fei Ke, Ya-Ping Guo, Chang-Qing Zhang, Wei -Tao Jia
Summary: This study investigates the regulatory mechanism of osteoporotic bone regeneration using multifunctional SrFe12O19-LDH/CS scaffolds, providing a theoretical basis for the research, development, and clinical application of this scaffold on osteoporotic bone defects.
MATERIALS TODAY BIO
(2022)
Article
Engineering, Biomedical
Yu-Wei Ge, Min Chu, Zi-Yang Zhu, Qin-Fei Ke, Ya-Ping Guo, Chang-Qing Zhang, Wei -Tao Jia
Summary: A novel CEF/Fe3O4/HA/CS/PQQ layered scaffold with magnetically oriented micro-cellulose fibres was developed. The scaffold exhibited enhanced compressive properties and could suppress inflammatory response and osteoclast differentiation, while promoting new blood vessel formation and collagen-I fibre processing.
MATERIALS TODAY BIO
(2022)
Article
Engineering, Biomedical
Zhe Liu, Qian Tang, Ruo-Tao Liu, Ming-Zhao Yu, Hao Peng, Chang-Qing Zhang, Zhen-Zhong Zhu, Xiao-Juan Wei
Summary: This article investigates the molecular mechanism and preventive strategies for implant failure in patients using long-term glucocorticoids. A customized implant targeting WNT16 was constructed using a self-assembly coating, which was found to promote bone cell growth and integration while also exhibiting antibacterial properties.
BIOACTIVE MATERIALS
(2023)
Correction
Cell & Tissue Engineering
Peng Ding, Qiyuan Tan, Zhanying Wei, Qiyu Chen, Chun Wang, Luyue Qi, Li Wen, Changqing Zhang, Chen Yao
Article
Biochemistry & Molecular Biology
Pei Liu, Youshui Gao, Pengbo Luo, Hongping Yu, Shang Guo, Fuyun Liu, Junjie Gao, Jianzhong Xu, Shengdian Wang, Changqing Zhang
Summary: Steroid-induced osteoporosis is fueled by immune cells involved in innate inflammatory responses, especially classical monocytes. Depletion of these cells can prevent bone loss and promote the growth of blood vessels that support bone formation.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
Article
Cell & Tissue Engineering
Peng Ding, Qiyuan Tan, Zhanying Wei, Qiyu Chen, Chun Wang, Luyue Qi, Li Wen, Changqing Zhang, Chen Yao
Summary: The study revealed that TLR9(-/-) mice exhibit low bone mass and chronic inflammation with increased levels of inflammatory cytokines, leading to enhanced osteoclastogenesis and bone loss. TLR9 deletion altered the gut microbiota, causing systemic inflammation and bone loss.
Review
Cell & Tissue Engineering
Lijun Wang, Xiuling You, Lingli Zhang, Changqing Zhang, Weiguo Zou
Summary: Bone remodeling is a lifelong process that involves a balance between bone formation and resorption. Mechanical forces play a crucial role in maintaining bone homeostasis, and loss of mechanical stimulation can weaken the bone structure and increase the risk of fracture. Understanding the mechanisms by which the body senses and transduces mechanical forces is important for studying bone disorders and developing strategies for skeletal rejuvenation.
