Article
Oncology
Rafaela Rossetti, Felipe Augusto Ros, Lucas Eduardo Botelho de Souza, Juliana de Matos Maconetto, Pericles Natan Mendes da Costa, Fernanda Ursoli Ferreira, Josiane Serrano Borges, Julianne Vargas de Carvalho, Nayara Patricia Morotti, Simone Kashima, Dimas Tadeu Covas
Summary: The bone marrow microenvironment may contain two distinct populations of mesenchymal stromal cells (MSC), one located perivascularly and the other in the endosteum. Recent research has focused on better understanding these MSC subsets in order to improve their clinical applications. Studies indicate that compact bone is a rich source of MSC and offers lower risk of contamination by hematopoietic cells. Compact bone-derived MSC also show higher osteogenic potential compared to bone marrow-derived MSC. These findings suggest the presence of functionally different MSC populations in bone marrow and compact bone.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Immunology
Chiara Tomasoni, Corinne Arsuffi, Samantha Donsante, Alessandro Corsi, Mara Riminucci, Andrea Biondi, Alice Pievani, Marta Serafini
Summary: Acute myeloid leukemia (AML) cells have a negative impact on the osteogenic differentiation of normal bone marrow stromal cells (BMSCs), and direct interaction between AML cells and BMSCs plays a vital role in this process. AML-mediated activation of Notch signaling is involved in the ineffective osteogenic commitment of BMSCs, resulting in the formation of a pre-osteoblast-rich niche supportive of leukemia development in the bone marrow.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Kai Dai, Qinghao Zhang, Shunshu Deng, Yuanman Yu, Fuwei Zhu, Shuang Zhang, YuanZhong Pan, Dandan Long, Jing Wang, Changsheng Liu
Summary: Constructing in vivo osteo-organoids by implanting bone morphogenetic protein-2-loaded scaffolds into the internal muscle pocket near the femur of mice supports the growth and subsequent harvest of therapeutically useful cells, and these cells demonstrate therapeutic potentials in cell therapies such as mitigating chronic liver fibrosis and reconstituting impaired immune organs.
Article
Multidisciplinary Sciences
Alexandra N. Rindone, Xiaonan Liu, Stephanie Farhat, Alexander Perdomo-Pantoja, Timothy F. Witham, Daniel L. Coutu, Mei Wan, Warren L. Grayson
Summary: Vascularization plays a critical role in cranial bone growth, maintenance, and healing. The study utilized a quantitative imaging platform to analyze the spatial relationship between blood vessels and skeletal progenitors in the murine calvarium, demonstrating specific vessel types are spatially correlated with skeletal progenitors during various cranial processes. Furthermore, the findings suggest a potential role for osteoclasts in maintaining the native cranial microvascular environment.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Chih-Yi Yang, Rita Jui-Hsien Lu, Ming-Kang Lee, Felix Shih-Hsian Hsiao, Ya-Ping Yen, Chun-Chun Cheng, Pu-Sheng Hsu, Yi-Tzang Tsai, Shih-Kuo Chen, I-Hsuan Liu, Pao-Yang Chen, Shau-Ping Lin
Summary: MSCs lacking DNMT3L exhibit reduced colony forming ability and impaired osteogenesis in vitro, with differentially regulated genes associated with bone morphology-related phenotypes. The observed defects are hypothesized to result from pre-deposited epigenetic signatures from DNMT3L-expressing progenitors, rather than a direct effect of missing DNMT3L in MSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biophysics
Zhibiao Bai, Kai Hu, Zeyu Shou, Han Zhou, Jiahuan Yu, Hongming Meng, Liangyan Chen, Tiantian Yu, Ruofei Lu, Na Li, Chun Chen
Summary: This study investigated the effect of PC/Mucin multilayers on the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). The results showed that PC/Mucin multilayers promoted the differentiation of BMSCs into osteoblasts and this effect was mediated by the activation of the Wnt/β-catenin pathway. These findings have important implications for the development of materials and coatings for bone regeneration.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Yuning Zhou, Yue Hu, Mamoru Uemura, Lunguo Xia, Xingge Yu, Yuanjin Xu
Summary: Strontium-substituted calcium silicate/silk fibroin composite materials have significant effects on the proliferation, osteogenic differentiation, and angiogenic factor secretion of rat bone marrow-derived mesenchymal stromal cells (rBMSCs), and can promote new bone formation in a rat calvarial defect model.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Dentistry, Oral Surgery & Medicine
Lucyene Miguita, Juliana Cristina de Souza, Victor Coutinho Bastos, Nubia Braga Pereira, Raphaela Alvarenga Braga de Freitas, Leticia Martins Guimaraes, Gleide Fernandes de Avelar, Luciana Oliveira Andrade, Walderez Ornelas Dutra, Fabio Daumas Nunes, Wagner Henriques Castro, Julio Cesar Tanos de Lacerda, Amanda Maria Sena Reis, Vanessa Fatima Bernardes, Marina Goncalves Diniz, Ricardo Santiago Gomez, Carolina Cavalieri Gomes
Summary: The study evaluated the mutational status of mononuclear cells and giant cells in CGCG, as well as the osteogenic potential of stromal cells. The results confirm that the signature mutations are restricted to stromal cells in CGCG, and these cells have the capacity to differentiate into osteoblasts.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2022)
Review
Endocrinology & Metabolism
Yuki Matsushita, Wanida Ono, Noriaki Ono
Summary: Bone marrow contains precursor cells for osteoblasts and adipocytes. Recent studies have identified subsets of bone marrow stromal cells (BMSCs) that can differentiate into marrow adipocytes and possess diverse functions in homeostasis and regeneration. The trajectory of BMSCs towards marrow adipocytes is influenced by multiple modes of cell-autonomous and non-cell autonomous regulations.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell & Tissue Engineering
Rose Ann G. Franco, Eamonn McKenna, Pamela G. Robey, Ross W. Crawford, Michael R. Doran, Kathryn Futrega
Summary: For the use of bone marrow stromal cells (BMSC) in cartilage repair, controlling their hypertrophic differentiation is crucial. TGF-beta 1 stimulation triggers chondrogenic and hypertrophic differentiation in BMSCs, and silencing SP7, a transcription factor associated with hypertrophic differentiation, reduces both hypertrophic and chondrogenic differentiation processes. However, SP7 silencing compromises overall trilineage differentiation of BMSCs and makes it challenging to promote chondrogenesis while reducing hypertrophy in cartilage tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Cell & Tissue Engineering
Ana Rita Pereira, Drenka Trivanovic, Philipp Stahlhut, Maximilian Rudert, Jurgen Groll, Marietta Herrmann
Summary: The fate and behavior of BM-MSC are influenced by their microenvironment. In this study, four different 2D cell-based matrices and a 3D decellularized human trabecular-bone scaffold were used to evaluate BM-MSC behavior. The cell-derived matrices were reliable for analyzing osteogenic differentiation, while the decellularized bone scaffold showed selective expression of bone-specific proteins. The complex architecture and physico-chemical properties of the scaffold upregulated stemness associated genes, indicating the conservation of naive features of BM-MSC in vitro.
JOURNAL OF TISSUE ENGINEERING
(2022)
Article
Oncology
Bianca Nowlan, Elizabeth D. Williams, Michael Robert Doran
Summary: The study evaluated the impact of partially humanizing mouse bone marrow with human bone marrow-derived stromal cells on human prostate cancer cell behavior. Results showed that PC-3-DsRed cells preferentially populated bones in mice that had received human BMSC, and there were more bone lesions in these mice.
Article
Cell Biology
Graziana Monaco, Yann D. Ladner, Alicia J. El Haj, Nicholas R. Forsyth, Mauro Alini, Martin J. Stoddart
Summary: The study investigated the impact of different culture conditions on the differentiation of human bone marrow mesenchymal stromal cells (hBMSCs) towards cartilage and bone. The results showed that chondrogenic differentiation exhibited better performance compared to osteogenic differentiation under different culture conditions.
Article
Biology
Stephan A. Eisler, Filipa Curado, Gisela Link, Sarah Schulz, Melanie Noack, Maren Steinke, Monilola A. Olayioye, Angelika Hausser
Article
Biochemistry & Molecular Biology
Antje Jensch, Yannick Frey, Katharina Bitschar, Patrick Weber, Simone Schmid, Angelika Hausser, Monilola A. Olayioye, Nicole E. Radde
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Review
Biochemistry & Molecular Biology
Julia von Blume, Angelika Hausser
Review
Cell Biology
Monilola A. Olayioye, Bettina Noll, Angelika Hausser
Article
Gastroenterology & Hepatology
Shuya Zhang, Huan Liu, Meimei Yin, Xiuying Pei, Angelika Hausser, Eri Ishikawa, Sho Yamasaki, Zheng Gen Jin
Article
Oncology
Wolfgang S. Lieb, Cristiana Lungu, Raluca Tamas, Hannah Berreth, Philipp Rathert, Peter Storz, Monilola A. Olayioye, Angelika Hausser
INTERNATIONAL JOURNAL OF CANCER
(2020)
Article
Biochemistry & Molecular Biology
Ines Mueller, Elwira Strozyk, Sebastian Schindler, Stefan Beissert, Htoo Zarni Oo, Thomas Sauter, Philippe Lucarelli, Sebastian Raeth, Angelika Hausser, Nader Al Nakouzi, Ladan Fazli, Martin E. Gleave, He Liu, Hans-Uwe Simon, Henning Walczak, Douglas R. Green, Jiri Bartek, Mads Daugaard, Dagmar Kulms
Article
Cell Biology
Natalia Pacheco-Fernandez, Mehrshad Pakdel, Birgit Blank, Ismael Sanchez-Gonzalez, Kathrin Weber, Mai Ly Tran, Tobias Karl-Heinz Hecht, Renate Gautsch, Gisela Beck, Franck Perez, Angelika Hausser, Stefan Linder, Julia von Blume
JOURNAL OF CELL BIOLOGY
(2020)
Article
Cell Biology
Carlos O. Oueslati Morales, Attila Ignacz, Norbert Bencsik, Zsofia Sziber, Aniko Erika Ratkai, Wolfgang S. Lieb, Stephan A. Eisler, Attila Szucs, Katalin Schlett, Angelika Hausser
Summary: The PKD family promotes AMPAR endocytosis in primary hippocampal neurons, affecting synaptic plasticity. Inhibition of PKD increases AMPAR levels at synapses, slows down their endocytic trafficking, and enhances neuronal network activity. Conversely, ectopic expression of constitutive active PKD reduces synaptic AMPAR levels but increases their colocalization with early endosomes.
Article
Cell Biology
Cathrin Hagenlocher, Robin Siebert, Bruno Taschke, Senait Wieske, Angelika Hausser, Markus Rehm
Summary: Prolonged ER stress can trigger cell death, but the mechanism differs between cancer cells and pancreatic beta cells. This study found that ER stress-induced apoptosis in pancreatic beta cells does not involve TRAIL-R2, unlike in cancer cells. These findings have implications for targeted treatment strategies that need to consider the ER stress susceptibility of different cell types.
CELL DEATH DISCOVERY
(2022)
Article
Multidisciplinary Sciences
Ronja Reinhardt, Kai Hirzel, Gisela Link, Stephan A. Eisler, Tanja Hagele, Matthew A. H. Parson, John E. Burke, Angelika Hausser, Thomas A. Leonard
Summary: Phosphorylation is a common mechanism in cell signaling, and protein kinases are often regulated by phosphorylation. However, PKD regulates itself through dimerization-mediated trans-autoinhibition, followed by autophosphorylation, to control its activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Attila Ignacz, Domonkos Nagy-Herczeg, Angelika Hausser, Katalin Schlett
Summary: The actin cytoskeleton is the main regulator of synapse formation and maintenance. Different actin probes were compared in cultured hippocampal neurons to study their effectiveness in visualizing actin dynamics. The results showed that LifeAct-GFP and AC-GFP had similar affinity to actin, but they couldn't detect subtle changes in actin remodeling as effectively as EGFP-actin. Additionally, LifeAct-GFP had lower enrichment in dendritic spines compared to the other probes.
MOLECULAR BIOLOGY OF THE CELL
(2023)
Article
Oncology
Sophia Steinlein, Frank Essmann, Amanda Franceschini Ghilardi, Heike Horn, Julia Schueler, Angelika Hausser, Lijun Sun, German Ott, Claudia Kalla
Summary: In this study, two indolyl-chalcones (CIT-026 and CIT-223) were found to be highly effective in inhibiting the growth and viability of malignant pleural mesothelioma (MPM) cells, while having minimal effects on normal fibroblasts. These compounds induce tumor cell apoptosis by disrupting microtubule assembly and show promising potential as small-molecule therapeutics for MPM, especially in cells resistant to standard therapeutics.
FRONTIERS IN ONCOLOGY
(2023)
Review
Cell Biology
Elena Gutierrez-Galindo, Zeynep Hazal Yilmaz, Angelika Hausser
Summary: Protein kinase D (PKD) is a serine/threonine kinase family that plays a crucial role in the secretory pathway at the trans-Golgi network. Abnormal expression of PKD isoforms is mainly found in breast cancer, where it promotes growth, invasion, survival, and stem cell maintenance. This review discusses the isoform-specific functions of PKD in breast cancer progression, specifically focusing on the connection between PKD-regulated cellular processes and deregulated membrane trafficking and secretion. The challenges of targeting PKD as a therapeutic approach to prevent breast cancer progression are also highlighted.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Serena Capasso, Lucia Sticco, Riccardo Rizzo, Marinella Pirozzi, Domenico Russo, Nina A. Dathan, Felix Campelo, Josse van Galen, Maarit Holtta-Vuori, Gabriele Turacchio, Angelika Hausser, Vivek Malhotra, Isabelle Riezman, Howard Riezman, Elina Ikonen, Chiara Luberto, Seetharaman Parashuraman, Alberto Luini, Giovanni D'Angelo