Article
Cell & Tissue Engineering
Weiwei Xue, Caixia Fan, Bing Chen, Yannan Zhao, Zhifeng Xiao, Jianwu Dai
Summary: Transplantation of neural stem cells shows promise for restoring communication in spinal cord injury, but the inhibitory microenvironment often leads to glial differentiation rather than neuronal differentiation. Functional biomaterials can mitigate the adverse effects of the SCI microenvironment and promote neuronal differentiation of NSCs.
Article
Cell & Tissue Engineering
Kyung-Joo Seong, Seungho Choi, Hyun-Gwan Lee, Joon Haeng Rhee, Jin Ho Lee, Jeong-Tae Koh, Sun-Hun Kim, Won-Seok Choi, Ji-Yeon Jung, Won-Jae Kim
Summary: Toll-like receptor 5 plays a crucial role in regulating neurogenesis from mouse embryonic stem cells and adult hippocampal neural stem cells, promoting neural differentiation and inhibiting cell proliferation. TLR5 deficiency impairs fear memory performance in mice.
Article
Biochemistry & Molecular Biology
Marta Cadenas-Martin, Francisco Arnalich-Montiel, Maria P. De Miguel
Summary: This study developed a protocol for deriving human limbal stem cells (LSCs) from adipose-derived mesenchymal stem cells (ADSCs) that is compatible with the standard carrier human amniotic membrane. The best protocol achieved optimal differentiation efficiency and will benefit patients with LSC deficiency, aiding in the restoration of vision.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Sergi Angel Bonilla-Pons, Shoma Nakagawa, Elena Garreta Bahima, Alvaro Fernandez-Blanco, Martina Pesaresi, Justin Christopher D'Antin, Ruben Sebastian-Perez, Daniela Greco, Eduardo Dominguez-Sala, Raul Gomez-Riera, Rafael Ignacio Barraquer Compte, Mara Dierssen, Nuria Montserrat Pulido, Maria Pia Cosma
Summary: This study discovered that cell fusion can occur between human müller glia and adult stem cells in the human retina, resulting in partial regeneration of neurons. The results suggest that cell fusion-mediated therapy may be a potential regenerative approach for treating human retinal dystrophies.
Article
Biotechnology & Applied Microbiology
Dan Zhu, Zheming Cao, Xiaoyang Pang, Wei Jiang, Chihao Li, Xing Zhang, Xibin Tian, Haijun Tu, Panfeng Wu, Hemin Nie
Summary: Researchers have found that astrocytes (ASTs) in mice can form cell spheres on agarose surfaces and exhibit stem cell-like features. The derived A-iSCs also have the ability to differentiate into neurons and facilitate nerve regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xin Gao, Fang Hong, Zhenyu Hu, Zilong Zhang, Yang Lei, Xiaoyun Li, Tao Cheng
Summary: ABC portal is a database and web portal that contains 198 single-cell transcriptomic datasets related to the development, differentiation, and disorders of blood/immune cells. It provides interactive analysis modules and customized analysis functions, facilitating the exploration and re-analysis of single-cell data by researchers and clinicians.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Martina Zamponi, Peter A. Mollica, Yara Khodour, Julie S. Bjerring, Robert D. Bruno, Patrick C. Sachs
Summary: This study combines 3D bioprinting technology with hydrogels containing porcine brain extracellular matrix (BMX) to investigate the influence of ECM on stem cell differentiation. The results show that BMX favors neural differentiation of stem cells compared to standard basement membrane hydrogels and that these 3D bioprinted neural structures can be successfully transplanted into mice, where they continue to grow.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Filipa F. Ribeiro, Filipa Ferreira, Rui S. Rodrigues, Rita Soares, Diogo M. Pedro, Marta Duarte-Samartinho, Rita I. Aroeira, Elisabete Ferreiro, Jorge Valero, Susana Sola, Helena Mira, Ana M. Sebastiao, Sara Xapelli
Summary: The activation of A(2A) receptors promotes neural stem cell self-renewal, protects committed neuronal cells from cell death, and increases the density of immature and mature neuronal cells. Furthermore, the endogenous activation of A(2A) receptors is essential for BDNF-mediated cell proliferation and neuronal differentiation.
Review
Pharmacology & Pharmacy
Edward J. Calabrese
Summary: This paper provides a detailed assessment of hormetic dose responses in adipose-derived stem cells (ADSCs) of animal models and humans, showing that hormetic dose responses are commonly reported for ADSCs and may have a determinant role in potential clinical applications.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Engineering, Biomedical
Zahra Sadat Ghazali, Mahnaz Eskandari, Shahin Bonakdar, Philippe Renaud, Omid Mashinchian, Shahriar Shalileh, Fabien Bonini, Ilker Uckay, Olivier Preynat-Seauve, Thomas Braschler
Summary: Cell-imprinting technology is a novel method that directs stem cell fate by using substrates molded from target cells. The study demonstrated that cell-imprinted substrates can accelerate neural differentiation and induce morphological changes in stem cells, leading to upregulation of neural stem cell and early neuronal markers.
Review
Cell Biology
Davide Cinat, Robert P. Coppes, Lara Barazzuol
Summary: This review discusses how DNA damage affects the maintenance and function of stem cells in adult tissues and neoplasms, leading to inflammatory responses and changes in the microenvironment. DNA damage can induce cell senescence, increase pro-inflammatory factors release, and modulate immune responses, potentially impacting tissue homeostasis and cancer development. Understanding the DNA damage-induced immunomodulatory responses on stem cell microenvironment may provide insights into age-related diseases and cancer, offering novel treatment strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Linlin Liang, Chunhui Sun, Ruitong Zhang, Shuwei Han, Jingang Wang, Na Ren, Hong Liu
Summary: The research proposes a simple method to induce neural differentiation of stem cells using ultrasound-driven piezoelectric materials to generate electrical signals on the surface, allowing stem cells to differentiate into neurons without the need for biological or chemical factors. The assessment at the gene and protein levels confirms the differentiation of stem cells into neuron-like cells.
Article
Biochemical Research Methods
Jiang Qian, Yadi Wang, Xueling Li, Junhong Lu
Summary: The stem cell microenvironment is crucial for regulating cell proliferation and differentiation. This study utilizes synchrotron radiation-based Fourier transform infrared microspectroscopy to examine the combined effects of physical and chemical factors on stem cell differentiation at the single-cell level. The results demonstrate the distinct effects of low-concentration lithium and Wnt5a on human mesenchymal stem cells in a polyvinyl alcohol (PVA) hydrogel, highlighting the important role of niche signals in the Wnt pathway. These findings emphasize the significance of the microenvironment in chemical-induced effects on stem cell differentiation and provide a label-free, noninvasive method to identify niche function in stem cell biology.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Cell Biology
Xiaoli Huang, Hongfeng Guo, Xuejun Cheng, Jinyu Zhang, Wenzheng Qu, Qianyun Ding, Qihang Sun, Qiang Shu, Xuekun Li
Summary: Nicotinamide adenine dinucleotide hydrate (NAD+) plays a crucial role in regulating adult neural stem/progenitor cells (aNSPCs) by reducing proliferation, neuronal differentiation, and inducing apoptosis. NAD+ exposure also inhibits neuronal morphological development. Mechanistically, NAD+ exposure alters transcriptome and decreases the expression of genes related to ATP metabolism and the PI3k-Akt signaling pathway.