Article
Multidisciplinary Sciences
Xiaoyu Wei, Sulei Fu, Hanbo Li, Yang Liu, Shuai Wang, Weimin Feng, Yunzhi Yang, Xiawei Liu, Yan-Yun Zeng, Mengnan Cheng, Yiwei Lai, Xiaojie Qiu, Liang Wu, Nannan Zhang, Yujia Jiang, Jiangshan Xu, Xiaoshan Su, Cheng Peng, Lei Han, Wilson Pak-Kin Lou, Chuanyu Liu, Yue Yuan, Kailong Ma, Tao Yang, Xiangyu Pan, Shang Gao, Ao Chen, Miguel A. Esteban, Huanming Yang, Jian Wang, Guangyi Fan, Longqi Liu, Liang Chen, Xun Xu, Ji-Feng Fei, Ying Gu
Summary: This study used Stereo-seq technology to analyze spatial distribution and molecular features of axolotl brain during development and regeneration. The researchers identified an injury-induced ependymoglial cell cluster as a potential progenitor cell population for replenishing lost neurons. They also discovered regressed neurons and provided evidence for the involvement of resident ependymoglial cells in neurogenesis.
Editorial Material
Cell & Tissue Engineering
Stefano Pluchino, Alexandra M. Nicaise
Summary: Adult neural stem cells can oscillate between activated and dormant states in response to systemic TNF-a and its receptors, TNFRII and TNFRI, as identified by Belenguer et al. in Cell Stem Cell.
Article
Multidisciplinary Sciences
John P. Gleeson, Namit Chaudhary, Katherine C. Fein, Rose Doerfler, Patricia Hredzak-Showalter, Kathryn A. Whitehead
Summary: Breast milk contains cells that are beneficial for infant development, but little is known about these cells. This study characterized the cells in mature breast milk from healthy donors and identified the main cell population as epithelial cells, with several subpopulations including previously unidentified ones. These findings provide insights into the potential applications of breast milk cells in maternal and infant medicine.
Article
Engineering, Biomedical
Yujung Chang, Byounggook Cho, Euiyeon Lee, Junyeop Kim, Junsang Yoo, Jung-Suk Sung, Youngeun Kwon, Jongpil Kim
Summary: Adult neurogenesis is a lifelong process generating new neurons in the dentate gyrus, but this capacity decreases with age and is associated with cognitive and memory decline. Electromagnetized gold nanoparticles were found to promote adult hippocampal neurogenesis, improve cognitive function, and offer a potential therapeutic strategy for age-related brain diseases.
Article
Biochemistry & Molecular Biology
Adela Bernabeu-Zornoza, Raquel Coronel, Charlotte Palmer, Alberto Martin, Victoria Lopez-Alonso, Isabel Liste
Summary: This study analyzes the effects of monomeric A beta 40 on the biology of human neural stem cells (NSCs). The results show that high concentrations of A beta 40 can induce cell death and DNA damage in NSCs, while also promoting cell proliferation and neurogenesis. These findings contribute to the understanding of Alzheimer's disease pathology and may have implications for the development of NSC-based therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Melanie Roussat, Thomas Jungas, Christophe Audouard, Sofiane Omerani, Francois Medevielle, Eric Agius, Alice Davy, Fabienne Pituello, Sophie Bel-Vialar
Summary: During cortical development, the cell cycle regulator CDC25B plays a crucial role in regulating the balance between different types of progenitor cells and their production of neurons. Deleting CDC25B in apical progenitors leads to an increase in TBR1(+) neurons at the expense of TBR2(+) basal progenitors, due to the lengthening of the G(2) phase.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Cell Biology
Yuan Cao, Hai-ning Wu, Xiu-li Cao, Kang-yi Yue, Wen-juan Han, Zi-peng Cao, Yu-fei Zhang, Xiang-yu Gao, Ceng Luo, Xiao-fan Jiang, Hua Han, Min-hua Zheng
Summary: Ttyh1 is essential for the stemness of neural stem cells (NSCs), with its deficiency leading to activated neurogenesis and enhanced spatial learning and memory in adult mice, and accelerated exhaustion of NSC pool and impaired neurogenesis in aged mice. The regulation of calcium signaling and involvement of transcription factor NFATc3 are critical mechanisms by which Ttyh1 affects quiescence versus activation of NSCs. Our research provides new targets for intervention to activate quiescent NSCs for injury repair during pathology and aging.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yubing Liu, Maria Bilen, Marie-Michelle McNicoll, Richard A. Harris, Bensun C. Fong, Mohamed Ariff Iqbal, Smitha Paul, Janice Mayne, Krystal Walker, Jing Wang, Daniel Figeys, Ruth S. Slack
Summary: Alzheimer's disease (AD) is a progressive neurodegenerative disorder leading to dementia. The hippocampus, one of the main sites for neurogenesis, exhibits significant neuronal loss in AD. Using the 3xTg mouse model, researchers discovered that neurogenic deficits are present during postnatal stages, even before the onset of neuropathology or behavioral deficits. Specifically, the 3xTg mice had fewer neural stem/progenitor cells, reduced proliferation, and decreased numbers of newborn neurons. The findings suggest that impairments in neurogenesis occur early in AD, opening up possibilities for early diagnosis and intervention.
CELL DEATH & DISEASE
(2023)
Review
Cell Biology
Patricia Coelho, Ligia Fao, Sandra Mota, A. Cristina Rego
Summary: Mitochondria play a crucial role in neurogenesis, influencing the fate of neural stem cells through their function and structure. Abnormalities in mitochondria are associated with neurodegenerative diseases, and stem cell therapies may alleviate these diseases by improving mitochondrial function and dynamics.
AGEING RESEARCH REVIEWS
(2022)
Article
Medicine, Research & Experimental
Nataya Sritawan, Apiwat Sirichoat, Anusara Aranarochana, Wanassanan Pannangrong, Peter Wigmore, Jariya Umka Welbat
Summary: Adult neurogenesis is the process of producing newborn neurons in the adult neural stem cells, which plays a role in learning and memory. Methotrexate (MTX), a chemotherapeutic drug, has negative effects on memory and hippocampal neurogenesis in animal models. Metformin, an antidiabetic drug with antioxidant properties, can alleviate the deteriorations caused by MTX in memory and hippocampal neurogenesis in adult rats. This study investigates the effects of metformin on neural stem cells, apoptosis biomarkers, and synaptogenesis proteins in the hippocampus of rats treated with MTX.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Medicine, Research & Experimental
Salinee Naewla, Ram Prajit, Nataya Sritawan, Kornrawee Suwannakot, Apiwat Sirichoat, Anusara Aranarochana, Peter Wigmore, Jariya Umka Welbat
Summary: This study investigated the effects of the flavonoid Hesperidin (Hsd), found in citrus fruits, on MTX-induced apoptosis of hippocampal neural stem cells. The results showed that Hsd can attenuate the impairments of hippocampal NSC proliferation and neuronal apoptosis caused by MTX.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Gastroenterology & Hepatology
Keiko Ishikawa, Shinya Sugimoto, Mayumi Oda, Masayuki Fujii, Sirirat Takahashi, Yuki Ohta, Ai Takano, Kazuhiro Ishimaru, Mami Matano, Kosuke Yoshida, Hikaru Hanyu, Kohta Toshimitsu, Kazuaki Sawada, Mariko Shimokawa, Megumu Saito, Kenta Kawasaki, Ryota Ishii, Koji Taniguchi, Takeshi Imamura, Takanori Kanai, Toshiro Sato
Summary: This study reveals the quiescent nature of human LGR5(+) colonic stem cells and their contribution to post-injury regeneration.
Article
Materials Science, Multidisciplinary
Hongfei Ge, Quan Hu, Tunan Chen, Yang Yang, Chao Zhang, Jun Zhong, Yi Yin, Xuheng Jiang, Xin Zhou, Shuhong Wang, Rong Hu, Wenyan Li, Hua Feng
Summary: A feasible method for modifying grafted neural stem cells (NSCs) using layer-by-layer (LbL) assembly has been developed, which enhances their regenerative ability and survival rate, showing potential application in the treatment of ischemic stroke.
APPLIED MATERIALS TODAY
(2022)
Article
Neurosciences
Dmitry I. Maltsev, Victor A. Aniol, Mariia A. Golden, Anastasia D. Petrina, Vsevolod V. Belousov, Natalia V. Gulyaeva, Oleg V. Podgorny
Summary: The delicate balance between quiescence and division of radial glia-like stem cells is crucial for adult hippocampal neurogenesis. This study demonstrates that long-term stimulation of quiescent RGLs using memantine or electroconvulsive seizure leads to premature depletion and altered neurogenesis. Additionally, aging affects the ability of quiescent RGLs to be recruited by these inducers.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Cell & Tissue Engineering
Claudia Colussi, Claudio Grassi
Summary: Nucleoporins play a crucial role in chromatin interaction and gene expression modulation, particularly in maintaining the balance of stemness and differentiation in stem cells. They are increasingly implicated in central nervous system functions, impacting neurogenesis, neurophysiology, and neurological disorders. However, the role of Nup-mediated epigenetic regulation in neural stem cells remains largely unexplored, with mechanisms of action still being unveiled.
Article
Nanoscience & Nanotechnology
Yurena Polo, Jon Luzuriaga, Jagoba Iturri, Igor Irastorza, Jose Luis Toca-Herrera, Gaskon Ibarretxe, Fernando Unda, Jose-Ramon Sarasua, Jose Ramon Pineda, Aitor Larranaga
Summary: A novel bioresorbable elastomeric scaffold with ordered nanopatterned topography and graphene oxide surface functionalization was developed, facilitating the adhesion, differentiation, and aligned migration of neural stem cells. This system has the potential to promote spatially oriented neural precursor cell connectivity, showing promise for future cellular therapy, including nerve tissue regeneration.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Review
Cell Biology
Margot Jarrige, Elie Frank, Elise Herardot, Sabrina Martineau, Annabelle Darle, Manon Benabides, Sophie Domingues, Olivier Chose, Walter Habeler, Judith Lorant, Christine Baldeschi, Cecile Martinat, Christelle Monville, Lise Morizur, Karim Ben M'Barek
Summary: The rapid progress in stem cell research has laid foundations for regenerative medicine, with growing evidence pointing towards paracrine effects being more impactful in therapy outcomes. Extracellular vesicles are being explored as potential cell-free therapeutic agents due to their ability to cross biological barriers and transfer bioactive molecules intercellularly. Evidence suggests that they could play a beneficial role in alleviating pathological conditions and addressing hurdles in regenerative medicine.
Editorial Material
Biology
Jose R. Pineda, Iker Badiola, Gaskon Ibarretxe
Review
Biochemistry & Molecular Biology
Jon Luzuriaga, Yurena Polo, Oier Pastor-Alonso, Beatriz Pardo-Rodriguez, Aitor Larranaga, Fernando Unda, Jose-Ramon Sarasua, Jose Ramon Pineda, Gaskon Ibarretxe
Summary: Human dental pulp stem cells (hDPSCs) are a promising stem cell type for regenerative therapies due to their ability to grow without serum and potential for autologous grafts. This review discusses the advantages of hDPSCs for neuroregenerative cell therapies, exploring their embryonic origin, postnatal niche, differentiation potential, neuronal differentiation issues, neuroprotective and immunomodulatory capacities, as well as their integration with scaffold materials to enhance functionality in injured nervous systems. Perspectives on current and future applications of hDPSCs in neuroregenerative cell therapies are also provided.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gaelle Pennarun, Julien Picotto, Laure Etourneaud, Anna-Rita Redavid, Anais Certain, Laurent R. Gauthier, Paula Fontanilla-Ramirez, Didier Busso, Caroline Chabance-Okumura, Benoit Theze, Francois D. Boussin, Pascale Bertrand
Summary: The study reveals that overexpression of lamin B1 disrupts the shelterin complex, leading to telomere instability, telomeric fusions, and telomere losses in human cells. Additionally, new interactions between lamin B1 and shelterin proteins may play a crucial role in telomere stability.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biology
Benoit Roch, Vincent Abramowski, Olivier Etienne, Stefania Musilli, Pierre David, Jean-Baptiste Charbonnier, Isabelle Callebaut, Francois D. Boussin, Jean-Pierre de Villartay
Summary: The development of a new Xrcc4(M61R) separation of function mouse line demonstrated the importance of XRCC4 in stabilizing DNA ligase IV in V(D)J recombination. Functional interplays between XRCC4 and PAXX, ATM, and Xlf were identified, with significant impacts on mouse immune system development. These findings provide new insights into the clinical manifestations of human XRCC4-deficient condition.
Article
Biochemical Research Methods
S. Olaechea-Lazaro, I Garcia-Santisteban, J. R. Pineda, I Badiola, S. Alonso, Jose Ramon Bilbao, Nora Fernandez-Jimenez
Summary: shinyCurves is an online shiny-based software that analyzes qRT-PCR amplification data from multi-plate and multi-platform formats. It does not require programming experience and can call samples Positive, Negative or Undetermined for viral infection, providing melting and amplification curve plots for visual inspection of results. This flexible, integrative and user-friendly software speeds up analysis of massive qRT-PCR data, with the option to automatically produce and evaluate melting and amplification curve plots.
BMC BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Irene Romayor, Lara Herrera, Maria Buron, Myriam Martin-Inaraja, Laura Prieto, Jone Etxaniz, Marta Ingles-Ferrandiz, Jose Ramon Pineda, Cristina Eguizabal
Summary: The study demonstrates that under naive culture NHSM medium conditions, human hiPSCs and hESCs exhibit higher viability and expression of naive pluripotency markers, and are more conducive to cell differentiation into endoderm and mesoderm.
Article
Biology
Nerea Garcia-Urkia, Jon Luzuriaga, Veronica Uribe-Etxebarria, Igor Irastorza, Francisco Javier Fernandez-San-Argimiro, Beatriz Olalde, Nerea Briz, Fernando Unda, Gaskon Ibarretxe, Iratxe Madarieta, Jose Ramon Pineda
Summary: Obesity has a negative impact on human lifespan and is a significant public health issue worldwide. However, the development of in vitro 3D adipose tissue models is limited and expensive. In this study, we designed and produced 3D solid foams using a mixture of collagen and decellularized adipose tissue to mimic the in vivo adipose microenvironment. We demonstrated that these foams are compatible with dental pulp stem cell culture and can support adipocyte differentiation.
Article
Oncology
Isaline Bontemps, Celine Lallemand, Denis Biard, Nathalie Dechamps, Thierry Kortulewski, Emmanuelle Bourneuf, Capucine Siberchicot, Francois Boussin, Sylvie Chevillard, Anna Campalans, Jerome Lebeau
Summary: CD24 plays a crucial role in breast cancer cells, and loss of its expression induces acquisition of stemness properties and increases resistance to radiation and chemotherapy. CD24 controls therapeutic resistance of breast cancer cells by regulating ROS levels.
Editorial Material
Oncology
Ignacio Niechi, Jose R. R. Pineda
FRONTIERS IN ONCOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Veronica Uribe-Etxebarria, Jose Ramon Pineda, Patricia Garcia-Gallastegi, Alice Agliano, Fernando Unda, Gaskon Ibarretxe
Summary: The dental pulp of permanent human teeth contains stem cells known as Dental Pulp Stem Cells (DPSCs), which have the ability to differentiate into multiple cell lineages. The pluripotency core factors expressed by these cells, as well as the involvement of signaling pathways like Notch and Wnt, contribute to their stemness maintenance. Utilizing recombinant proteins, pharmacological modulators, serum-free media, and appropriate scaffolds, researchers can optimize the potency of DPSCs without genetic modification. This review highlights the mechanisms and regulatory pathways responsible for maintaining stemness in DPSCs, drawing interesting parallels with pluripotent stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Amandine Bery, Olivier Etienne, Laura Mouton, Sofiane Mokrani, Christine Granotier-Beckers, Laurent R. Gauthier, Justyne Feat-Vetel, Thierry Kortulewski, Elodie A. Peres, Chantal Desmaze, Philippe Lestaveal, Vilma Barroca, Antony Laugeray, Fawzi Boumezbeur, Vincent Abramovski, Stephane Mortaud, Arnaud Menuet, Denis Le Bihan, Jean-Pierre de Villartay, Francois D. Boussin
Summary: XLF/Cernunnos is a crucial component of the ligation complex involved in cNHEJ, a DNA repair pathway. In Xlf-/- mice, neurodevelopmental delays, behavioral alterations, microcephaly, and apoptosis of neural cells are observed, resembling clinical features in humans deficient in cNHEJ. Premature neurogenesis in these mice is caused by asymmetric chromosome segregation, suggesting a link between chromosome dynamics and neurodevelopmental pathologies associated with NHEJ deficiency or genotoxic stress.
Article
Cell & Tissue Engineering
J. Luzuriaga, P. Garcia-Gallastegui, N. Garcia-Urkia, Jr. Pineda, I. Irastorza, F-J Fernandez-San-Argimiro, B. Olalde, F. Unda, I Madarieta, G. Ibarretxe
Summary: 3D cell culture systems based on biological scaffold materials from animal and human tissues show promising applications in cell therapy and personalised medicine. The extracellular matrix from white adipose tissue is a potential biomaterial for tissue engineering. Porcine ECM scaffolds effectively support the osteogenic differentiation of human dental pulp stem cells, while human ECM scaffolds are more suitable for adipogenesis.
EUROPEAN CELLS & MATERIALS
(2022)
Article
Biology
L. Mouton, O. Etienne, J. Feat-Vetel, D. A. Barriere, E. A. Peres, F. Boumezbeur, F. D. Boussin, D. Le Bihan
Summary: In utero exposure to ionizing radiation can lead to long-term effects on the microstructure of brain tissue, as demonstrated by MRI imaging in mice. This study highlights the potential for combining anatomical and diffusion MRI to investigate the radiosensitivity of local brain areas noninvasively.
RADIATION RESEARCH
(2021)
