Article
Engineering, Biomedical
Yun Chang, Ramizah Syahirah, Stephanie N. Oprescu, Xuepeng Wang, Juhyung Jung, Scott H. Cooper, Sandra Torregrosa-Allen, Bennett D. Elzey, Alan Y. Hsu, Lauren N. Randolph, Yufei Sun, Shihuan Kuang, Hal E. Broxmeyer, Qing Deng, Xiaojun Lian, Xiaoping Bao
Summary: This study demonstrates the successful generation of AGM-like hematopoietic system from human pluripotent stem cells through temporal manipulation of Wnt signaling. The generated hematopoietic progenitor cells resemble primary cord blood HSCs at the transcriptional level and exhibit lymphoid and myeloid potential in vitro. The cells also show the ability to home to a definitive hematopoietic site, rescue bloodless zebrafish, and engraft and repopulate in mouse recipients.
Article
Biochemistry & Molecular Biology
Frauke Gotzhein, Tim Aranyossy, Lars Thielecke, Tanja Sonntag, Vanessa Thaden, Boris Fehse, Ingo Mueller, Ingmar Glauche, Kerstin Cornils
Summary: In this study, a murine model of HSCT was used to investigate the contribution of different hematological subpopulations to the reconstitution process and the impact of age on this process. The results showed that hematopoietic stem cells and multipotent progenitors played a major role in reconstitution, independent of age. The reconstitution patterns were stable in all age groups. These findings support the expansion of HSCT in older individuals as a promising strategy for treating hematological diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Valentina Capo, Sara Penna, Ivan Merelli, Matteo Barcella, Serena Scala, Luca Basso-Ricci, Elena Draghici, Eleonora Palagano, Erika Zonari, Giacomo Desantis, Paolo Uva, Roberto Cusano, Lucia Sergi Sergi, Laura Crisafulli, Despina Moshous, Polina Stepensky, Katarzyna Drabko, Zuhre Kaya, Ekrem Unal, Alper Gezdirici, Giuseppe Menna, Marta Serafini, Alessandro Aiuti, Silvia Laura Locatelli, Carmelo Carlo-Stella, Ansgar S. Schulz, Francesca Ficara, Cristina Sobacchi, Bernhard Gentner, Anna Villa
Summary: Allogeneic hematopoietic stem cell transplantation is the preferred treatment for autosomal recessive osteopetrosis caused by TCIRG1 gene defects. However, some patients are not eligible due to disease severity. Utilizing circulating CD34(+) cells for gene therapy shows promise in overcoming limitations of stem cell harvest in osteopetrotic patients, potentially shaping future gene-based treatments for skeletal diseases.
Review
Cell Biology
Stefania Crippa, Ludovica Santi, Margherita Berti, Giada De Ponti, Maria Ester Bernardo
Summary: The human organism requires the production of approximately 1 trillion new blood cells per day through hematopoiesis in the bone marrow, regulated by the BM microenvironment and HSPCs. The BM niche, defined by interactions between HSPCs and non-hematopoietic cells, controls HSPC maintenance and orchestrates hematopoiesis according to the body's needs. Mesenchymal stromal cells (MSCs) play a key role in the BM niche by regulating HSPC homeostasis and impacting the outcome of hematopoietic stem cell transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Hal E. Broxmeyer, Larry L. Luchsinger, Rona Singer Weinberg, Alexandra Jimenez, Emeline Masson Frenet, Wouter vant Hof, Maegan L. Capitano, Christopher D. Hillyer, Mark H. Kaplan, Scott Cooper, James Ropa
Summary: Long-term cryopreserved cord blood units retain similar numbers of hematopoietic stem and progenitor cells compared with fresh and recently cryopreserved units, and contain highly functional cells. These units demonstrate robust engraftment in mouse transplantation models, and gene program differences can potentially serve as markers for engraftment.
CELL REPORTS MEDICINE
(2023)
Article
Cell & Tissue Engineering
Jianan Jiang, Jinhua Qin, Jisheng Li, Xiaosong Lin, Bowen Zhang, Zeng Fan, Lijuan He, Quan Zeng, Wen Yue, Min Zheng, Xuetao Pei, Yanhua Li
Summary: This study found that the small molecule Ricolinostat can promote the differentiation of megakaryocytic cells from HSPCs, increase the proliferation of megakaryocytic cells, and decrease the secretion of IL-8 and the expression of CXCR2, thereby improving the efficiency of megakaryocytic differentiation of HSPCs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Ximena Bonilla, Ana Milena Lara, Manuela Llano-Leon, David A. Lopez-Gonzalez, David G. Hernandez-Mejia, Rosa Helena Bustos, Bernardo Camacho-Rodriguez, Ana-Maria Perdomo-Arciniegas
Summary: Umbilical cord blood is an important source of hematopoietic stem and progenitor cells, and recent research has focused on developing ex vivo expansion strategies for these cells. Co-culturing the cells with specific mesenchymal stromal cells has been found to significantly increase their cellular dose and regulate their proliferation capacity. Additionally, the secretory profile of umbilical cord blood-derived cells closely resembles that of bone marrow-derived cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Lifei Hou, Koichi Yuki
Summary: CD11a plays a critical role in leukocyte arrest and immunological synapse formation, but its role in the bone marrow is not well studied. This research showed that CD11a is expressed on all subsets of hematopoietic stem and progenitor cells (HPSCs). CD11a deficiency enhances HSPCs activity under LPS stimulation, possibly by upregulating the production of IL-27 to promote cell proliferation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Diana Sa da Bandeira, Alastair Morris Kilpatrick, Madalena Marques, Mario Gomez-Salazar, Telma Ventura, Zaniah Nashira Gonzalez, Dorota Stefancova, Fiona Rossi, Matthieu Vermeren, Chris Sebastiaan Vink, Mariana Beltran, Neil Cowan Henderson, Bongnam Jung, Reinier van der Linden, Harmen Jan George van de Werken, Wilfred F. J. van Ijcken, Christer Betsholtz, Stuart John Forbes, Henar Cuervo, Mihaela Crisan
Summary: This study reveals the expression of PDGFRβ in distinct perivascular stromal cell layers in mice and shows that its deletion impairs hematopoiesis. The study also demonstrates that PDGFRβ(+) cells play a dual role in murine hematopoiesis, acting as support for HSPCs in the aortic niche and giving rise to a subset of HSPCs that persist into adulthood.
Review
Medicine, General & Internal
Christi T. Salisbury-Ruf, Andre Larochelle
Summary: Homology-directed gene editing in HSPCs holds promise for treating inherited blood disorders by overcoming the limitations of viral vector-mediated gene therapies. However, achieving high-efficiency gene editing in these cells remains a technical challenge that requires further strategies and advancements.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biology
Daniel E. Morales-Mantilla, Bailee Kain, Duy Le, Anthony R. Flores, Silke Paust, Katherine Y. King
Summary: This study suggests that hematopoietic stem and progenitor cells (HSPCs) have the potential to be used as a new therapeutic strategy for sepsis. Infusion of HSPCs in mice with sepsis resulted in improved survival rates and reduced inflammation. These findings highlight the important immunomodulatory role of HSPCs and their potential for treating sepsis.
Article
Biochemistry & Molecular Biology
You Jung Hwang, Dong-Yeop Shin, Min-Jung Kim, Hyosun Jang, Soyeon Kim, Hyunwon Yang, Won Il Jang, Sunhoo Park, Sehwan Shim, Seung Bum Lee
Summary: This study found that SR1 could alleviate radiation-induced hematopoietic injury. It increased the survival rate and promoted the recovery of peripheral blood cell counts in irradiated mice. In vitro experiments also showed that SR1 increased the population of human hematopoietic stem/progenitor cells, reduced radiation-induced DNA damage and apoptosis. These results suggest that SR1 may be a potential radiomitigator for hematopoietic injury.
Article
Engineering, Biomedical
Wenjing Li, Haiwei Liang, Yanxiao Ao, Baixue Tang, Junyang Li, Ning Li, Jianwei Wang, Yanan Du
Summary: Hematopoietic stem cells (HSCs) have the ability to differentiate into all blood cells and immune cells. By simulating the bone marrow microenvironment, we designed degradable scaffolds to regulate the fate of HSCs. In vivo transplantation confirmed the importance of the scaffolds in maintaining hematopoietic function, providing a foundation for parameter design in 3D HSC culture systems.
Article
Multidisciplinary Sciences
Runfeng Miao, Harim Chun, Xing Feng, Ana Cordeiro Gomes, Jungmin Choi, Joao P. Pereira
Summary: This study reveals that a complex homeostatic balance between hematopoietic stem cells (HSCs) and hematopoietic progenitor cells is maintained through competition for a limited amount of cell signaling molecule. When early hematopoietic progenitors fail to interact with specific cells, HSC numbers increase.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Rui Wang, Xiaoqi Wang, Shijie Yang, Yunshuo Xiao, Yanhui Jia, Jiangfan Zhong, Qiangguo Gao, Xi Zhang
Summary: This study found that human umbilical cord-derived mesenchymal stem cells (hUCMSCs) can effectively decrease the incidence and severity of GVHD by enriching myeloid-derived suppressor cells in GVHD target tissues. RNA sequencing results showed that hUCMSCs highly expressed CXCL1.
Article
Biotechnology & Applied Microbiology
Tomoyuki Shimazu, Mai Suzuki, Ryo Takasaki, Asuka Besshi, Yuna Suzuki, Yoichiro Iwakura
Summary: This study investigated the relationship between lactic acid bacteria (LAB) and innate immune receptors. The researchers found that C-type lectin receptors (CLRs) are involved in the immune-stimulating function of LAB. The interaction between LAB and CLRs can reduce the levels of certain cytokines and is only observed in heat-killed LAB. These findings contribute to a better understanding of the immunomodulatory effects of LAB.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biochemistry & Molecular Biology
Shohei Iida, Takehisa Nakanishi, Fumiyasu Momose, Masako Ichishi, Kento Mizutani, Yoshiaki Matsushima, Ai Umaoka, Makoto Kondo, Koji Habe, Yoshifumi Hirokawa, Masatoshi Watanabe, Yoichiro Iwakura, Yoshihiro Miyahara, Yasutomo Imai, Keiichi Yamanaka
Summary: This study investigates the pathophysiology of amyloidosis secondary to cutaneous inflammation using a mouse model of dermatitis, and evaluates the therapeutic effects of Janus kinase (JAK) inhibitors and IL-17A ablation. The results show that skin-derived inflammatory cytokines can induce amyloid deposition in the liver and spleen, and that JAK inhibitors and IL-17A ablation can reduce amyloidosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Yasush Adachi, Masanori Nojima, Mitsuru Mori, Toshiyuki Kubo, Noriyuki Akutsu, Yasushi Sasaki, Hiroshi Nakase, Yingsong Lin, Youichi Kurozawa, Kenji Wakai, Akiko Tamakoshi
Summary: This study analyzed the association between serum sFas levels and the incidence of liver cancer. The results showed that high levels of sFas were associated with an increased risk of liver cancer. These findings suggest that sFas may serve as a biomarker for assessing liver cancer risk.
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2023)
Article
Toxicology
Takashi Ashino, Yuki Nakamura, Hirokazu Ohtaki, Yoichiro Iwakura, Satoshi Numazawa
Summary: In the study of polymicrobial sepsis, pro-inflammatory cytokines play a vital role in regulating hepatic drug transporter expression. The research found that both CLP and LPS can reduce the expression of drug transporters, but IL-6 is the major factor in the downregulation of hepatic multidrug transporters caused by polymicrobial sepsis.
TOXICOLOGY LETTERS
(2023)
Article
Hematology
Makoto Otsu, Yuji Tanabe, Ayako Iwakiri, Kazuna Arima, Anna Uchiyama, Marina Yamamoto, Shinichi Ohtani, Hiroshi Endo, Mina Komoto, Koji Miyazaki
Summary: This study aimed to establish an FC method for accurate detection of AB antigen expression in RBCs, addressing the technical difficulties presented by ABO subtypes. The optimized fixatives successfully prevented agglutination and maintained low autofluorescence, enabling precise analysis of AB antigens in RBCs. This modified protocol provides a reliable assay applicable to the characterization of rare weak ABO variants.
Article
Anesthesiology
Aya Watahabe, Naoki Shimada, Hiroto Ishiki, Noriko Fujiwara, Masanori Nojima, Arinobu Tojo
Summary: Younger age and pancreatic cancer are associated with higher requirements of fentanyl patch in terminally ill cancer patients. Patients with pancreatic cancer require more frequent adjustment of the medication dosage near death.
JOURNAL OF PAIN & PALLIATIVE CARE PHARMACOTHERAPY
(2023)
Correction
Oncology
Chunyan Yu, Xiulong Niu, Yongrui Du, Yan Chen, Xiaomei Liu, Lingling Xu, Yoichiro Iwakura, Xiaoxia Ma, Yan Li, Zhi Yao, Weimin Deng
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2023)
Article
Oncology
Mariko Watanabe, Hiromi Hatsuse, Kazuaki Nagao, Makoto Nakashima, Kaoru Uchimaru, Makoto Otsu, Koji Miyazaki, Ryouichi Horie
Summary: This study investigated the link between CD30 and the characteristics of cHL cells. CD30 stimulation increased multinucleated cells and induced chromosomal imbalances and mitotic errors. CD30 induced DNA double-strand breaks, chromatin bridges, and multinucleated cells through ROS generation, linking CD30 to the morphological features and genetic complexity of cHL cells.
Article
Immunology
Takashi Ashino, Yuki Nakamura, Hirokazu Ohtaki, Yoichiro Iwakura, Satoshi Numazawa
Summary: Sepsis affects drug metabolism and the expression of cytochrome P450s. The study shows that cecal ligation and puncture (CLP) induces the expression of proinflammatory cytokines and leads to decreased survival in IL-6(-/-) mice. CLP also downregulates the expression of Cyp2c and Cyp3a11 genes, but this effect is abolished by IL-6 depletion.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Takehisa Nakanishi, Shohei Iida, Junko Maruyama, Hayato Urushima, Masako Ichishi, Yoshiaki Matsushima, Kento Mizutani, Yuichi Nakayama, Kyoko Sugioka, Mai Nishimura, Ai Umaoka, Yoichiro Iwakura, Makoto Kondo, Koji Habe, Daisuke Tsuruta, Osamu Yamamoto, Yasutomo Imai, Keiichi Yamanaka
Summary: The skin is a major immune organ that produces inflammatory cytokines in response to stimuli, leading to systemic inflammation. Inflammatory skin diseases like psoriasis and atopic dermatitis can cause vascular disorders like arteriosclerosis. However, the mechanism of arteriosclerosis in dermatitis and the role of cytokines are not fully understood. This study investigates the pathophysiology of arteriosclerosis and treatment options for inflammatory skin conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Public, Environmental & Occupational Health
Noriko Fujiwara, Naoki Shimada, Masanori Nojima, Keisuke Ariyoshi, Norie Sawada, Motoki Iwasaki, Shoichiro Tsugane
Summary: This study identified factors associated with home death in Japan, including being unmarried, unemployed male, and having a high drinking level in male. Cardiovascular disease, cerebrovascular disease, and external factors were significantly associated with home death, while the risk of home death for cancer patients tended to be lower.
JOURNAL OF EPIDEMIOLOGY
(2023)
Article
Immunology
Bunki Natsumoto, Hirofumi Shoda, Yasuo Nagafuchi, Mineto Ota, Takashi Okumura, Yumi Horie, Tomohisa Okamura, Kazuhiko Yamamoto, Motonori Tsuji, Makoto Otsu, Hideki Taniguchi, Keishi Fujio
Summary: In this study, patient-derived induced pluripotent stem cells (iPSCs) were used in combination with genetic analysis to identify and elucidate the biological functions of rare variants underlying systemic lupus erythematosus (SLE). It was found that a rare variant in the OASL gene was shared between SLE-iPSCs and another independent SLE patient, and there was a significant accumulation of OASL variants among SLE patients. Additionally, genome editing experiments confirmed the impact of these variants on type 1 interferon secretion in immune cells.
JOURNAL OF AUTOIMMUNITY
(2023)
Article
Gastroenterology & Hepatology
Kenji Watanabe, Masanori Nojima, Hiroshi Nakase, Toshiyuki Sato, Minoru Matsuura, Nobuo Aoyama, Taku Kobayashi, Hirotake Sakuraba, Masakazu Nishishita, Kaoru Yokoyama, Motohiro Esaki, Fumihito Hirai, Masakazu Nagahori, Sohachi Nanjo, Teppei Omori, Satoshi Tanida, Yoshihiro Yokoyama, Kei Moriya, Atsuo Maemoto, Osamu Handa, Naoki Ohmiya, Kiichiro Tsuchiya, Shinichiro Shinzaki, Shingo Kato, Toshio Uraoka, Hiroki Tanaka, Noritaka Takatsu, Atsushi Nishida, Junji Umeno, Masanao Nakamura, Yoshiyuki Mishima, Mikihiro Fujiya, Kenji Tsuchida, Sakiko Hiraoka, Makoto Okabe, Takahiko Toyonaga, Katsuyoshi Matsuoka, Akira Andoh, Yoshio Hirota, Tadakazu Hisamatsu, J COMBAT Study Grp
Summary: The degree of immune response to COVID-19 vaccination in IBD patients varies. Most IBD patients showed a sufficient immune response, while some had persistently low response. Factors such as age and medication were associated with the immune response.
JOURNAL OF GASTROENTEROLOGY
(2023)
Review
Immunology
Motoki Amai, Masanori Nojima, Yoshikazu Yuki, Hiroshi Kiyono, Fumitaka Nagamura
Summary: To assess safety in vaccine development, stricter grading scales are required. However, concern exists that their strictness may lead to an overestimation of some adverse events. An analysis of a phase I clinical trial of a preventive vaccine revealed that the occurrence of certain adverse events differed when assessed using FDA grading scale compared to reference intervals. The criteria for hypernatremia and hypokalemia were lower and higher, respectively, than most standards.
Article
Oncology
Yasushi Adachi, Masanori Nojima, Mitsuru Mori, Hiro-o Yamano, Yasushi Sasaki, Hiroshi Nakase, Yingsong Lin, Kenji Wakai, JACC Study
Summary: The findings suggest that serum SOD activity is inversely correlated with the risk of colorectal carcinoma, particularly in men and individuals with rectal cancer or left-sided CRC.
CANCER EPIDEMIOLOGY
(2023)