Article
Cell Biology
Soo-Yeon Woo, Hansong Lee, Su Min Park, Hee-Seon Choi, Jayoung Kim, Munju Kwon, Jihyung Sohn, Ji Ho Nam, Hyung-Sik Kim, Parkyong Song, Ninib Baryawno, Yun-Hak Kim, Koanhoi Kim, Dongjun Lee
Summary: 27HC, an oxygenated derivative of cholesterol, regulates the quantity and function of various cell populations in the bone marrow and may serve as a new therapeutic target for hematological malignancies. Exogenous 27HC treatment induces oxidative stress and apoptosis in the bone marrow.
CELL DEATH & DISEASE
(2022)
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Hematology
Vincenzo Calvanese, Hanna K. A. Mikkola
Summary: Developmental hematopoiesis is a complex process involving the generation of differentiated blood cells and the establishment of a pool of undifferentiated hematopoietic stem cells (HSCs) for postnatal life. Recent single-cell studies have identified rare human HSCs and traced their origin to a unique type of arterial endothelium in the aorta-gonad-mesonephros region. These studies provide new insights into HSC generation and may inform efforts to generate HSCs from pluripotent stem cells in vitro.
Review
Biochemistry & Molecular Biology
Nattaya Damkham, Surapol Issaragrisil, Chanchao Lorthongpanich
Summary: This review provides an update on the regulation of YAP and TAZ, transcriptional coactivators in the Hippo signaling pathway, in stem cell behaviors and differentiation. It highlights their roles in mechanosensing and mechanotransduction, as well as the importance of understanding how cells perceive and respond to their microenvironment for improving the efficiency and efficacy of stem cell products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Alexandra Backstrom, David Yudovich, Kristijonas Zemaitis, Ludvig Nilsen Falck, Agatheeswaran Subramaniam, Jonas Larsson
Summary: This study utilized the CRISPR/Cas9 system to perform multiplexed gene editing in primary human cells and successfully achieved double knockout of cell surface molecules. Furthermore, they demonstrated the system's ability to model gene dependencies and combinatorial effects for cell survival and HSPC expansion. These findings are important for studying genetic dependencies and cooperation, as well as investigating underlying mechanisms of gene interactions.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
S. S. Hoepner, Ana Raykova, R. Radpour, M. A. Amrein, D. Koller, G. M. Baerlocher, C. Riether, A. F. Ochsenbein
Summary: The study demonstrates that LIGHT and its receptor LT beta R promote quiescence and self-renewal of HSCs, while LT beta R deficiency contributes to survival in a mouse leukemia model.
NATURE COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Youn Keong Cho, Hyun-Kyung Kim, Soon Sung Kwon, Su-Hee Jeon, June-Won Cheong, Ki Taek Nam, Han-Soo Kim, Sinyoung Kim, Hyun Ok Kim
Summary: To address the blood shortage issue, scientists have been exploring the use of human-induced pluripotent stem cells (hiPSCs) to generate red blood cells (RBCs) in vitro. In this study, hiPSCs were established from peripheral blood, cord blood, and bone marrow, and it was found that peripheral blood and cord blood were the most reliable sources for RBC production in vitro. These findings will aid in the selection of optimal hiPSC lines for clinical RBC production in the future.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Cell Biology
Zoya Mann, Manisha Sengar, Yogesh Kumar Verma, Raja Rajalingam, Pawan Kumar Raghav
Summary: Hematopoietic stem cells possess the properties of self-renewal and differentiation, which can be used for treating various hematological disorders. However, the self-renewal capacity of HSCs decreases with increasing passages, so more research on ex vivo expansion is needed to enhance their self-renewal ability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Bai Ling, Yunyang Xu, Siyuan Qian, Ze Xiang, Shihai Xuan, Jian Wu
Summary: Hematopoietic stem cells (HSCs) play a crucial role in the hematopoietic system as they can self-renew and differentiate into various blood cells. The mTOR signaling pathway is an important regulator of HSCs' differentiation, self-renewal, and quiescence, and several molecules can modulate these potentials by influencing this pathway. This review discusses the regulation of HSCs' three potentials by the mTOR signaling pathway and highlights potential regulators. Additionally, the clinical significance of studying this regulation and future predictions are also outlined.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Stefania Crippa, Ludovica Santi, Margherita Berti, Giada De Ponti, Maria Ester Bernardo
Summary: The human organism requires the production of approximately 1 trillion new blood cells per day through hematopoiesis in the bone marrow, regulated by the BM microenvironment and HSPCs. The BM niche, defined by interactions between HSPCs and non-hematopoietic cells, controls HSPC maintenance and orchestrates hematopoiesis according to the body's needs. Mesenchymal stromal cells (MSCs) play a key role in the BM niche by regulating HSPC homeostasis and impacting the outcome of hematopoietic stem cell transplantation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jacqueline Feyen, Zhen Ping, Lanpeng Chen, Claire van Dijk, Tim V. D. van Tienhoven, Paulina M. H. van Strien, Remco M. Hoogenboezem, Michiel J. W. Wevers, Mathijs A. Sanders, Ivo P. Touw, Marc H. G. P. Raaijmakers
Summary: Myeloid cells reduce the function of HSCs through interferon signaling via a neutrophil-NK cell dependent process.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
James L. C. Che, Daniel Bode, Iwo Kucinski, Alyssa H. Cull, Fiona Bain, Hans J. Becker, Maria Jassinskaja, Melania Barile, Grace Boyd, Miriam Belmonte, Andy G. X. Zeng, Kyomi J. Igarashi, Juan Rubio-Lara, Mairi S. Shepherd, Anna Clay, John E. Dick, Adam C. Wilkinson, Hiromitsu Nakauchi, Satoshi Yamazaki, Berthold Gottgens, David G. Kent
Summary: The use of Fgd5 reporter mouse and EPCR surface marker allows for the exclusive identification of HSCs from non-HSCs in expansion cultures, revealing a gene expression signature that can identify functional HSCs from multiple cellular states.
Article
Biology
Sisi Zhang, Rachel W. S. Chan, Ernest H. Y. Ng, William S. B. Yeung
Summary: Notch signaling plays a key role in maintaining the quiescent state of endometrial mesenchymal stromal/stem cells (eMSC), and is influenced by Notch and Wnt signaling from the microenvironment.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Weizhen Jia, Lingyu Kong, Hiroyasu Kidoya, Hisamichi Naito, Fumitaka Muramatsu, Yumiko Hayashi, Han-Yun Hsieh, Daishi Yamakawa, Daniel K. Hsu, Fu-Tong Liu, Nobuyuki Takakura
Summary: This study demonstrates that Gal-3 expression in long-term repopulating HSCs is induced in response to Tie2 and Mpl, and plays a crucial role in maintaining HSC quiescence through regulation of p21.
NATURE COMMUNICATIONS
(2021)
Article
Hematology
Emanuele Gioacchino, Cansu Koyunlar, Joke Zink, Hans de Looper, Madelon de Jong, Tomasz Dobrzycki, Christopher B. Mahony, Remco Hoogenboezem, Dennis Bosch, Paulina M. H. van Strien, Martin E. van Royen, Pim J. French, Eric Bindels, Kirsten J. Gussinklo, Rui Monteiro, Ivo P. Touw, Emma de Pater
Summary: Through studying Gata2b-deficient zebrafish, this research demonstrates the role of GATA2 in hematopoiesis, showing a decrease in myeloid lineage differentiation and a shift towards lymphoid lineage differentiation in the absence of Gata2b.
