Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 87, Issue -, Pages 77-85Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2011.11.012
Keywords
DNA and RNA components; Silver nanoparticles; Surface charge; SERS
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Funding
- U.S. Department of Defense
- DHS Center of Excellence for Explosives
- U.S. Department of Homeland Security [2008-ST-061-ED0001]
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This work focused on establishing the parameters for enhancing the Raman signals of DNA and RNA constituents: nitrogenous bases, nucleosides and nucleotides, using metallic nanoparticles as surface enhanced Raman scattering substrates. Silver nanospheres were synthesized using sodium borohydride as a reducing agent and sodium citrate as a capping agent. The prepared nanoparticles had a surface plasmon band at similar to 384 nm and an average size of 12 +/- 3 nm. The nanoparticles' surface charge was manipulated by changing the pH of the Ag colloidal suspensions in the range of 1-13. Low concentrations as 0.7 mu M were detected under the experimental conditions. The optimum pH values were: 7 for adenine, 9 for AMP, 5 for adenosine, 7 for dAMP and 11 for deoxyadenosine. (C) 2011 Elsevier B.V. All rights reserved.
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