Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 78, Issue 1, Pages 243-247Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2010.10.001
Keywords
Gold nanoparticle (AuNPs); alpha-Fetoprotein (AFP); Fluorescence excitation spectrum; Quenching; \Local field effect
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Funding
- National High-tech R&D Program (863 program) [2009AA04Z314]
- Fundamental Research Funds for the Central Universities [xjj20100049]
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The effect of colloid gold nanoparticles (AuNPs) on the fluorescence excitation spectrum of alpha-fetoprotein (AFP) has been investigated experimentally. The excitation spectral peaks of AFP with low concentration from 0.01 ng ml(-1) to 12 ng ml(-1) increase monotonically with increasing of AFP concentration. When some gold colloids were added to the AFP solution, the excitation peak at 285 nm decreases distinctly. By comparing the excitation peak intensity of AFP solution with gold colloids and without gold colloids at different AFP concentrations, the quenching effect from gold nanoparticle was more effective at lower AFP concentration. So the range of concentration from 0.01 ng ml(-1) to 0.09 ng ml(-1) will be the potential range of applications because of the higher sensitivity. The physical origin based on local field effect was investigated to illuminate this local environment dependent fluorescence quenching. The changing extent of quenching with different AFP concentrations can be attributed to the nonlinear decreasing of the local field factor of gold nanoparticles as a function of environmental dielectric constant. (C) 2010 Elsevier B.V. All rights reserved.
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