Article
Cell Biology
Wenbo Zhou, Michael Graner, Petr Paucek, Cheryl Beseler, Matthew Boisen, Andrew Bubak, Francisco Asturias, Woro George, Arin Graner, David Ormond, Timothy Vollmer, Enrique Alvarez, Xiaoli Yu
Summary: Grey matter pathology is crucial in the development of multiple sclerosis (MS). MS plasma immunoglobulin G (IgG) antibodies, particularly IgG1, form large aggregates (>100 nm) that are retained after binding to Protein A. These IgG aggregates induce significantly higher levels of neuronal apoptosis compared to other neurological disorders and healthy donors. The apoptosis is mediated by complement activation and the presence of IgG, complement activation products, and active caspase 3 in treated neuronal cells. MS plasma cytotoxic antibodies are not present in Protein G flow-through or paired plasma. The size of MS IgG aggregates is larger than 100 nm. Our findings suggest that MS IgG antibodies play a pathological role in complement activation, axonal damage, and neurodegeneration through apoptosis.
CELL DEATH & DISEASE
(2023)
Article
Multidisciplinary Sciences
Mariam Movsisyan, Armine Chopikyan, Irina Kasparova, Gohar Hakobjanyan, Fabrice Carrat, Meline Sukiasyan, Marieta Rushanyan, Mariam Chalabyan, Sanobar Shariff, Burhan Kantawala, Anna Keshishyan, Alvard Hovhannisyan, Armine Hakobyan, Gayane Petrosyan, Armen Minasyan, Armen Muradyan, Arsene Mekinian, Konstantin Yenkoyan
Summary: The comprehension of long-term humoral immune response against SARS-CoV-2 is crucial for the treatment and vaccination strategies of COVID-19. In this study, we found that the majority of convalescent patients had durable antibodies lasting more than a year, and the antibody levels were correlated with disease severity and age.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Physical
Paul C. Arpin, Daniel B. Turner
Summary: Transient circular dichroism (TCD) spectroscopy is an emerging time-resolved method that can probe the electronic transitions and dynamic aspects of chirality. In this study, the authors develop a theoretical description of TCD for electronic multi-level models and simulate and analyze TCD spectra for three representative model systems. The presence and detection of spectral signatures of electronic coherences are discussed in detail.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Multidisciplinary Sciences
Ana Rita Cruz, Maurits A. den Boer, Jurgen Strasser, Seline A. Zwarthoff, Frank J. Beurskens, Carla J. C. de Haas, Piet C. Aerts, Guanbo Wang, Rob N. de Jong, Fabio Bagnoli, Jos A. G. van Strijp, Kok P. M. van Kessel, Janine Schuurman, Johannes Preiner, Albert J. R. Heck, Suzan H. M. Rooijakkers
Summary: Immunoglobulin G (IgG) molecules are essential in the human immune response against bacterial infections, with an important effector being the induction of complement activation. Staphylococcal protein A (SpA) has been identified as a virulence factor of Staphylococcus aureus that effectively blocks IgG hexamerization and subsequent complement activation. This study demonstrates that SpA competes with IgG monomers to block the formation of IgG hexamers, thus preventing downstream complement activation on the surface of S. aureus.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Emily R. Rhodes, Jonathan G. Faris, Brian M. Petersen, Kayla G. Sprenger
Summary: Through molecular dynamics simulations, this study found that framework mutations can significantly alter the distribution of VH-VL interface angles, which in turn affect the local flexibility of antibodies by changing the solvent accessible surface area. These findings emphasize the limitations of static antibody crystal structures and demonstrate the dynamic nature of antibodies in solution. The study further highlights the importance of framework mutations in antibody structure and lays the foundation for establishing design principles to create antibodies with increased specificity, stability, and breadth.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Chemistry, Physical
Beibei Zhou, John T. Tobin, Stephan Drusch, Sean A. Hogan
Summary: The interfacial properties of dairy proteins are of great interest to the food industry. Various environmental conditions and processes significantly affect the structure and stability of food materials. Further studies may help in designing better interfacial protein structures for different food applications.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Analytical
Thuy Tran, Erik Martinsson, Sergio Vargas, Ingemar Lundstrom, Carl-Fredrik Mandenius, Daniel Aili
Summary: The production of therapeutic monoclonal antibodies is a complex process that requires extensive analytical characterization. Aggregation of antibodies is common and problematic, as it can affect the product quality and immunogenicity. Rapid detection of aggregates is challenging with current analytical techniques.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Arnulf Stein, Daniela Rolf, Christian Lotze, Sascha Feldmann, David Gerbert, Benjamin Guenther, Andreas Jeindl, Johannes J. Cartus, Oliver T. Hofmann, Lutz H. Gade, Katharina J. Franke, Petra Tegeder
Summary: Through experimental methods and theoretical simulations, the electronic properties of 1,3,8,10-tetraazaperopyrene derivatives adsorbed on Au(111) were investigated. The results showed weak physisorption of the molecular layers and hybridization of molecular states with the Au(111) substrate. These findings are important for the design of materials with organic-metal interfaces having a low charge injection barrier.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Biochemistry & Molecular Biology
Jeffrey S. Cross, Yasuhiro Kubota, Abhijit Chatterjee, Samir Unni, Toshiyuki Ikoma, Motohiro Tagaya
Summary: Experimental and multi-scale molecular modeling approaches were used to study the adsorption of FBG on LNO surfaces, showing an increase in adsorption with increasing solution concentration independent of charges. The presence of solvated medium influenced the adsorption phenomenon due to the negative surface potential.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Brayan F. Garcia, Soheil Saraji
Summary: This study examines interfacial rheology and polar component dynamics in crude oil and brine systems with different salinities. It reveals that diffusion/adsorption processes are the dominant relaxation mechanisms and transient interfacial rheology plays a crucial role in defining final apparent interfacial properties.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Chemistry, Multidisciplinary
Takahiro Iwata, Hisaaki Hirose, Kentarou Sakamoto, Yusuke Hirai, Jan Vincent V. Arafiles, Misao Akishiba, Miki Imanishi, Shiroh Futaki
Summary: The Fc region binding peptide can assist in delivering immunoglobulin G into cells, forming particle-like liquid droplets; upon contact with the cellular membrane, the droplets spontaneously enter cells and distribute throughout; translocation of IgG is achieved through involvement of cellular machinery and membrane ruffling.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Emma B. A. Hinderink, Marcel B. J. Meinders, Reinhard Miller, Leonard Sagis, Karin Schroen, Claire C. Berton-Carabin
Summary: Protein blends are commonly used to stabilize emulsion products, but the competitive adsorption between proteins can lead to the displacement of initially adsorbed proteins, compromising the integrity and stability of the interfacial film. This review critically examines experimental techniques and modeling approaches to assess interfacial composition, properties, and mechanisms of protein displacement. The potential of Brownian dynamic simulations in describing interfacial displacement is also discussed.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Article
Thermodynamics
Dan Guo, Xuewen Cao, Lihui Ma, Pan Zhang, Yang Liu, Jiang Bian
Summary: This study systematically analyzed the density, interfacial characteristics, and adsorption of methane-heavy hydrocarbon mixtures. The results showed that the density of the heavy hydrocarbon-rich phase decreases with decreasing chain length and increasing pressure, while the methane-rich phase increases. When methane molecules dissolve into the bulk liquids, the heavy hydrocarbon chains stretch and the intermolecular separation distances increase. The interaction energy between the two molecules is the main cause of system swelling. The adsorption of methane improves with increasing pressure until reaching saturation. The strong adsorption of methane mainly occurs in the first surface layer in dilute solutions and becomes more pronounced with increasing methane fraction. For heavy hydrocarbons with shorter chains, the surface layers are narrower and the separation distances are reduced. Furthermore, methane molecules prefer to stay at the outer edge of the surface layers and are closer to the gas phase than heavy hydrocarbons by approximately 0.2-0.7 & ANGS;.
Article
Immunology
Augusta Horvath, Lisa Rogers, Georgios Pollakis, Olga Baranov, Nora Pieroth, Sarah Joseph, Mkunde Chachage, Asli Heitzer, Lucas Maganga, Frank Msafiri, Agricola Joachim, Edna Viegas, Leigh-Anne Eller, Hannah Kibuuka, Supachai Rerks-Ngarm, Punnee Pitisuttithum, Sorachai Nitayapan, Jittima Dhitavat, Nakorn Premsri, Sarah Fidler, Robin J. J. Shattock, Merlin Lee Robb, Jonathan Weber, Sheena McCormack, Patricia Jane Munseri, Eligius Lyamuya, Charlotta Nilsson, Arne Kroidl, Michael Hoelscher, Ralf Wagner, Christof Geldmacher, Kathrin Held
Summary: Immunogens and vaccination regimens can impact the recognition of immune-epitopes, with the potential to steer responses towards or away from vulnerable viral epitopes. This study examined HIV vaccine trials and found that the induction of V3-specific antibodies did not inhibit the targeting of other linear epitopes. Strong V3 targeting was associated with a stronger overall immune response and recognition of more antigenic regions, while V2 targeting was specific to certain immunogens. These findings suggest that the induction of antibodies against V3 and other vulnerable regions may not be mutually exclusive.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Physics, Multidisciplinary
Soumik Das, Noe Atzin, Xingzhou Tang, Ali Mozaffari, Juan de Pablo, Nicholas L. Abbott
Summary: Solitons in liquid crystals (LCs) can generate various interfacial hydrodynamic phenomena, such as droplet generation and jetting, when colliding with isotropic fluid interfaces. By manipulating key parameters, a universal state diagram characterizing the energy transduction from soliton flexoelectric energy to droplet interfacial energy is established. This study reveals the potential of using LC solitons to induce nonlinear electrohydrodynamic effects at fluid interfaces.
PHYSICAL REVIEW LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Jacopo Vialetto, Manos Anyfantakis, Sergii Rudiuk, Mathieu Morel, Damien Baigl
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Chemistry, Multidisciplinary
Pauline E. Galy, Sergii Rudiuk, Mathieu Morel, Damien Baigl
Article
Biochemistry & Molecular Biology
Anais Marconnet, Baptiste Michon, Christel Le Bon, Fabrice Giusti, Christophe Tribet, Manuela Zoonens
Article
Chemistry, Physical
Vincent Poulichet, Mathieu Morel, Sergii Rudiuk, Damien Baigl
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2020)
Review
Biotechnology & Applied Microbiology
Jorge Royes, Valerie Biou, Nathalie Dautin, Christophe Tribet, Bruno Miroux
MICROBIAL CELL FACTORIES
(2020)
Article
Chemistry, Multidisciplinary
Koyomi Nakazawa, Farah El Fakih, Vincent Jallet, Caroline Rossi-Gendron, Marina Mariconti, Lea Chocron, Mafumi Hishida, Kazuya Saito, Mathieu Morel, Sergii Rudiuk, Damien Baigl
Summary: User-defined DNA nanostructures can rapidly undergo a folding transition on a soft cationic substrate, forming compact structures or long ribbons, and can be switched back to their original shape by adding heparin. This reversible reconfiguration preserves structural details and functionality, and can be used to dynamically adjust the spatial distribution of tethered proteins.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Lucas Sixdenier, Christophe Tribet, Emmanuelle Marie
Summary: This study enhances conventional interfacial complexation in water-in-oil emulsions by introducing thermoresponsive poly(N-isopropylacrylamide) (PNIPAM), enabling efficient encapsulation of fragile biomacromolecules. The thermal collapse transition of PNIPAM strands leads to co-precipitation of nanoparticles and irreversible segregation in the interfacial polymer layer, forming gel-like mixed shells. The thermo-enhanced interfacial complexation holds promise for straightforward formation, strengthening, and functionalization of capsule shells, providing new opportunities for biomacromolecule delivery systems.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Marina Mariconti, Mathieu Morel, Damien Baigl, Sergii Rudiuk
Summary: Hybrid DNA-protein nanogels were successfully prepared with controllable size and density using a simple method involving highly biotinylated DNA as a backbone and streptavidin-biotin binding for cross-linking. The resulting nanogels could be adjusted in size and density by changing the amount of cross-linking streptavidin and the rate of DNA biotinylation. Incorporation of enzymes like AP, HRP, and beta Gal into the nanogels showed retained functional activity, with HRP activity slightly improved, indicating that the physical density of the functionalized DNA nanogels plays a crucial role in enhancing enzymatic activity.
Article
Chemistry, Multidisciplinary
Jacopo Vialetto, Sergii Rudiuk, Mathieu Morel, Damien Baigl
Summary: This study presents a generic method for optically reconfigurable colloidal crystals at the air/water interface and reveals a new mechanism for controlling lattice properties using light. The photoreversible melting process is driven by thermocapillary stress and increased particle diffusivity, allowing for dynamic patterns and multiresponsive assembly to be achieved with light and magnetic stimuli.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Physical
J. Royes, V. A. Bjornestad, G. Brun, T. Narayanan, R. Lund, C. Tribet
Summary: Photoswitchable surfactants are used in the design of light-responsive colloids and self-assemblies. This study investigates the intermediate lipid-surfactant assemblies formed during fast isothermal photo-triggered transitions. The experiments reveal that the transient assemblies detected during the photo-triggered kinetics are similar to the stationary states. Additionally, the reorganization speed of surfactant-enriched assemblies is faster than that of lipid-enriched ones.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Polymer Science
Lucas Sixdenier, Amelie Auge, Yue Zhao, Emmanuelle Marie, Christophe Tribet
Summary: Aqueous-core polymer capsules with an upper critical solution temperature (UCST) were successfully formed by surfactant-polymer interfacial complexation. The thermoresponsiveness of the polymer shell was studied using fluorescence microscopy, showing that the capsules with a balanced UCST grafts can entrap nanoparticles and have solid-like dynamics below the cloud point.
Article
Materials Science, Biomaterials
Barbara Jackova, Guillaume Mottet, Sergii Rudiuk, Mathieu Morel, Damien Baigl
Summary: This article describes a rapid and purification-free immunoassay method using coding DNA to express antibodies in picoliter drops and characterize their binding to antigens in situ.
Article
Nanoscience & Nanotechnology
Caroline Rossi-Gendron, Farah El Fakih, Laura Bourdon, Koyomi Nakazawa, Julie Finkel, Nicolas Triomphe, Lea Chocron, Masayuki Endo, Hiroshi Sugiyama, Gaetan Bellot, Mathieu Morel, Sergii Rudiuk, Damien Baigl
Summary: Using a magnesium-free buffer, DNA strands and proteins can self-assemble into user-defined nanostructures at room or physiological temperature, without the need for thermal annealing. This isothermal self-assembly is thermodynamically controlled, allows for multiple folding pathways, and results in highly reconfigurable nanostructures. This method expands the possibilities for shape and function in self-assembly and provides a foundation for adaptive nanomachines and nanostructure discovery through evolution.
NATURE NANOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Jacopo Vialetto, Sergii Rudiuk, Mathieu Morel, Damien Baigl
Article
Chemistry, Multidisciplinary
Masayuki Hayakawa, Jacopo Vialetto, Manos Anyfantakis, Masahiro Takinoue, Sergii Rudiuk, Mathieu Morel, Damien Baigl
Article
Chemistry, Physical
Yiping Yin, Zhe Wang, Hua Zou
Summary: This study presents a novel method for preparing dimpled polymer-silica nanocomposite particles using interfacial swelling-based seeded polymerization. The optimized conditions allow for a relatively high percentage of dimpled particles to be achieved.
Article
Chemistry, Physical
Brenden D. Hoehn, Elizabeth A. Kellstedt, Marc A. Hillmyer
Summary: Porous materials with nanometer-scale pores have important applications as nanoporous membranes. In this study, ABA triblock copolymers were used as precursors to produce nanoporous polymeric membranes (NPMs) in thin film form by degrading the end blocks. Polycyclooctene (PCOE) NPMs with tunable pore sizes were successfully prepared using solvent casting technique. Oxygen plasma etching was employed to improve the surface porosity and hydrophilicity of the membranes. This study provides a straightforward method to produce tough NPMs with high porosity and hydrophilic surface properties.
Article
Chemistry, Physical
Vladislav S. Petrovskii, Stepan I. Zholudev, Igor I. Potemkin
Summary: This article investigates the behavior of linear and ring polypeptide chains in aqueous solution and explores the properties of the complexes formed by these chains with oppositely charged surfactants. The results demonstrate that the complexes of linear supercharged unfolded polypeptides and the corresponding surfactants exhibit impressive adhesive properties.
Article
Chemistry, Physical
Merve Cevik, Serkan Dikici
Summary: Cardiovascular diseases are a leading cause of death globally, and vascular grafts are a promising treatment option. This study focuses on tissue-engineered vascular grafts (TEVGs) using decellularized parsley stems as a potential biomaterial. The decellularized parsley stems showed suitable properties for TEVGs, providing a suitable environment for human endothelial cells to form a pseudo endothelium. This study showcases the potential of using parsley stems for TEVGs.
Article
Chemistry, Physical
Gustavo A. Vasquez-Montoya, Tadej Emersic, Noe Atzin, Antonio Tavera-Vazquez, Ali Mozaffari, Rui Zhang, Orlando Guzman, Alexey Snezhko, Paul F. Nealey, Juan J. de Pablo
Summary: The optical properties of liquid crystals are typically controlled by electric fields. In this study, we investigate the effects of microfluidic flows and acoustic fields on the molecular orientation and optical response of nematic liquid crystals. We identify several previously unknown structures and explain them through calculations and simulations. These findings hold promise for the development of new systems combining sound, flow, and confinement.
Article
Chemistry, Physical
Xinjun Wu, Xin Guan, Shushu Chen, Jiangpeng Jia, Chongyi Chen, Jiawei Zhang, Chuanzhuang Zhao
Summary: This research presents a novel shape memory hydrogel with a remodelable permanent shape and programmable cold-induced shape recovery behavior. The hydrogel is prepared using specific treatment methods to achieve shape fixation by heating and shape recovery by cooling. Additionally, deformable devices can be obtained by assembling hydrogel blocks with different concentrations.
Article
Chemistry, Physical
Rebecca Hengsbach, Gerhard Fink, Ulrich Simon
Summary: This study examines the properties of DNA functionalized pNipmam microgels and pure pNipmam microgels at different concentrations of sodium chloride and in PBS solutions using temperature dependent H-1-NMR measurements. The results show that DNA modification affects the volume phase transition temperature and the addition of salt and PBS further enhances this effect.
Article
Chemistry, Physical
Ningyi Li, Junhong Li, Lijingting Qing, Shicheng Ma, Yao Li, Baohui Li
Summary: This paper investigates the self-assembly behavior of colloids with competing interactions under spherical confinement and finds that different ordered structures can be formed under different sized spherical confinements. Moreover, more perforated structures are formed in smaller spheres.