Review
Multidisciplinary Sciences
Simon Finn Mayer, Chan Cao, Matteo Dal Peraro
Summary: Evolution has discovered numerous ways to transport material across cell membranes. Protein assemblies are essential for the formation of channels and pores that allow molecules to enter and exit cells, contributing to the processes that sustain living organisms. These biological pores are now being utilized as molecular sensors to study biomolecules and have potential applications in life sciences and medicine.
Review
Chemistry, Physical
Nathan Meyer, Imad Abrao-Nemeir, Jean-Marc Janot, Joan Torrent, Mathilde Lepoitevin, Sebastien Balme
Summary: In the past two decades, solid state and polymer nanopores have emerged as attractive methods for protein sensing, offering high specificity and sensitivity. This review focuses on the technique of protein detection using resistive pulse and ionic diodes, discussing significant achievements in detecting peptides and proteins, as well as strategies for improving nanopore resolution and accuracy. Challenges such as protein adsorption and nanopore lifetime are also highlighted.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2021)
Article
Multidisciplinary Sciences
Mohammed F. Alawami, Filip Boskovic, Jinbo Zhu, Kaikai Chen, Sarah E. Sandler, Ulrich F. Keyser
Summary: In this study, 102 quartz glass nanopores with a diameter of 11-18 nm were fabricated using laser-assisted capillary pulling. The use cycles of the nanopores were improved by vacuum storage and minimal washing steps. The single-molecule biosensing capability of the nanopores over repeated use cycles was demonstrated through quantitative analysis of a DNA carrier.
Review
Chemistry, Analytical
Naren Das, Bhaswati Chakraborty, Chirasree RoyChaudhuri
Summary: Solid-state nanopores have the ability to detect proteins at a single-molecule level with high sensitivity, high throughput, and low cost. This review provides an overview of recent progress in the field of solid-state and biological nanopores for protein detection in complex analytes, discussing the advantages, challenges, and methods to reduce the detection limit.
Article
Nanoscience & Nanotechnology
Toshiyuki Tosaka, Koki Kamiya
Summary: Detection of single molecules using biological nanopores is a powerful method for analyzing DNA sequences and shapes, as well as diagnosing diseases. However, distinguishing between double-stranded DNA and three-way junction DNA using native biological nanopores is challenging. This study created a modified OmpG nanopore to detect various DNA structures.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Shuanghong Yan, Liying Wang, Xiaoyu Du, Shanyu Zhang, Sha Wang, Jiao Cao, Jinyue Zhang, Wendong Jia, Yuqin Wang, Panke Zhang, Hong-Yuan Chen, Shuo Huang
Summary: This study has developed an efficient and cost-effective protocol for preparing a variety of MspA mutants for nanopore insertion, sequencing, and other operations, reducing the costs and time associated with human operations.
Review
Chemistry, Multidisciplinary
Yanfang Wu, J. Justin Gooding
Summary: This review provides an overview of the concepts, fabrication, and applications of nanopore sensors, with a focus on their potential and recent developments in quantitative analysis.
CHEMICAL SOCIETY REVIEWS
(2022)
Review
Chemistry, Multidisciplinary
Xin Guan, Haijuan Li, Limei Chen, Guohua Qi, Yongdong Jin
Summary: A glass capillary-based nanopore (G-nanopore) with tapered tip and flexible surface modifications provides a powerful sensing platform for diverse biological measurements. It enables noninvasive single molecule and subcellular single cell detections and shows potential for cancer diagnosis and treatment applications. However, current bottlenecks limit its widespread use in clinical applications.
Article
Chemistry, Analytical
Yu Dai, Yiwei Zhang, Qun Ma, Meihua Lin, Xiaojin Zhang, Fan Xia
Summary: Solid-state nanopores have advantages such as stability and easy modification, and they find applications in various fields. In addition to functional modification of the inner wall, researchers have also focused on the functional elements on the outer surface.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Sohini Pal, Akshay Naik, Anjana Rao, Banani Chakraborty, Manoj M. Varma
Summary: We demonstrate the single-molecule sensing of G-quadruplex formation in target-bound aptamers using solid-state nanopores. By functionalizing the nanopores with DNA origami sheets containing the aptamer sequence, we provide a versatile configuration for nanopore-based single-molecule sensors. The DNA origami sheet also includes a pore that can modulate the translocation dynamics of the target molecule. Using this system, we successfully differentiate the formation of G-quadruplex in the well-studied thrombin aptamer.
ACS APPLIED NANO MATERIALS
(2022)
Article
Biophysics
Pengkun Xia, Jiawei Zuo, Pravin Paudel, Shinhyuk Choi, Xiahui Chen, Md Ashiqur Rahman Laskar, Jing Bai, Weisi Song, JongOne Im, Chao Wang
Summary: Solid-state nanopores have various applications in single-molecule biosensing and sequencing, but high capacitive noise from conventional silicon substrates limits their performance. A new approach using insulating sapphire wafers for nanopore membranes is proposed, demonstrating scalable formation of small membranes with low noise sensing capabilities.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Electrochemistry
Jiaqi Zuo, Ning-Ning Song, Jia Wang, Xian Zhao, Meng-Yuan Cheng, Qinyi Wang, Wen Tang, Zekai Yang, Kaipei Qiu
Summary: This review critically evaluates the strategies developed in the field of protein nanopore-based SMS over the last two decades, with a particular emphasis on the relationship between the size and charge of protein nanopores and their sensing capabilities, as well as innovative approaches to lift the performance of nanopore SMS.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Ren Ren, Maozhong Sun, Pratibha Goel, Shenglin Cai, Nicholas A. Kotov, Hua Kuang, Chuanlai Xu, Aleksandar P. Ivanov, Joshua B. Edel
Summary: Highly sensitive and selective molecular probes made from nanoparticles have been designed for single-molecule nanopore sensing, successfully resolving and detecting both single and paired nanoparticles for applications such as antigen/antibody detection and microRNA sequence analysis. This technology is expected to significantly contribute to the development of highly sensitive and selective strategies for disease diagnosis and screening without the need for sample processing or amplification, while requiring minimal sample volume.
ADVANCED MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Chaoming Gu, Zhoubin Yu, Xiaojie Li, Xin Zhu, Chuanhong Jin, Zhen Cao, Shurong Dong, Jikui Luo, Zhi Ye, Yang Liu
Summary: Solid-state nanopores are crucial in sensing single-biomolecules, but their ultra-short translocation time limits the acquisition of detailed information and further applications. Combining MoS2 and graphene, we fabricated sub-10 nm ultra-thin MoS2-graphene heterostructure nanopores that slowed down the passage of biomolecules. By testing dsDNA and BSA at the single-molecule level, we observed special signals indicating the shape change of BSA during the slow translocation process. These findings suggest the potential of MoS2-graphene heterostructure nanopores in slowing down translocation and acquiring more detailed information about biomolecules.
Article
Chemistry, Multidisciplinary
Yaxian Liu, Xiaoyi Wang, Giulia Campolo, Xiangyu Teng, Liming Ying, Joshua B. Edel, Aleksandar P. Ivanov
Summary: This study develops a strategy that combines nanopore-based sensing with molecular carriers to detect α-Synuclein oligomers directly in clinical samples, providing a new approach for the diagnosis and mechanism study of Parkinson's disease.
Article
Chemistry, Multidisciplinary
Gerhard Baaken, Ibrahim Halimeh, Laurent Bacri, Juan Pelta, Abdelghani Oukhaled, Jan C. Behrends
Article
Chemistry, Analytical
Conrad Weichbrodt, Harsha Bajaj, Gerhard Baaken, Jiajun Wang, Serap Guinot, Mohamed Kreir, Jan C. Behrends, Mathias Winterhalter, Niels Fertig
Review
Biochemistry & Molecular Biology
Franz F. Dressler, Ilona Bodi, Marius Menza, Robin Moss, Heiko Bugger, Christoph Bode, Jan C. Behrends, Gunnar Seemann, Katja E. Odening
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2017)
Article
Chemistry, Physical
Monasadat Talarimoghari, Gerhard Baaken, Ralf Hanselmann, Jan C. Behrends
EUROPEAN PHYSICAL JOURNAL E
(2018)
Article
Chemistry, Multidisciplinary
Gerhard Baaken, Norbert Ankri, Anne-Katrin Schuler, Juergen Ruehe, Jan C. Behrends
Review
Chemistry, Multidisciplinary
Jan C. Behrends
Article
Multidisciplinary Sciences
Fabien Piguet, Hadjer Ouldali, Francoise Discala, Marie-France Breton, Jan C. Behrends, Juan Pelta, Abdelghani Oukhaled
SCIENTIFIC REPORTS
(2016)
Article
Cardiac & Cardiovascular Systems
Katja E. Odening, Ilona Bodi, Gerlind Franke, Raphaela Rieke, Anna Ryan de Medeiros, Stefanie Perez-Feliz, Hannah Fuerniss, Lea Mettke, Konstantin Michaelides, Corinna N. Lang, Johannes Steinfurt, Naga Deepa Pantulu, David Ziupa, Marius Menza, Manfred Zehender, Heiko Bugger, Remi Peyronnet, Jan C. Behrends, Zoltan Doleschall, Axel Zur Hausen, Christoph Bode, Genevieve Jolivet, Michael Brunner
EUROPEAN HEART JOURNAL
(2019)
Article
Microbiology
Michaela Wenzel, Marina Rautenbach, J. Arnold Vosloo, Tjalling Siersma, Christopher H. M. Aisenbrey, Ekaterina Zaitseva, Wikus E. Laubscher, Wilma van Rensburg, Jan C. Behrends, Burkhard Bechinger, Leendert W. Hamoen
Article
Chemistry, Multidisciplinary
Renate Reiter, Ekaterina Zaitseva, Gerhard Baaken, Ibrahim Halimeh, Jan C. Behrends, Andreas Zumbuehl
Article
Biotechnology & Applied Microbiology
Hadjer Ouldali, Kumar Sarthak, Tobias Ensslen, Fabien Piguet, Philippe Manivet, Juan Pelta, Jan C. Behrends, Aleksei Aksimentiev, Abdelghani Oukhaled
NATURE BIOTECHNOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Tobias Ensslen, Kumar Sarthak, Aleksei Aksimentiev, Jan C. Behrends
Summary: The use of a protein nanopore is demonstrated to differentiate posttranslational modifications (PTMs) in proteins or peptides based on the positions of acetylated and methylated lysine residues. This method detects PTMs and their positions by sensing the shape of a fully entrapped peptide, eliminating the need for controlled translocation. This molecular shape-sensing principle offers a versatile, label-free, and high-throughput characterization of protein isoforms.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemical Research Methods
Tobias Ensslen, Jan C. Behrends
Summary: This study presents a novel chip-based array device that allows high-resolution optical measurements and voltage clamp electrical recordings for studying membranes and membrane proteins. The device enables optical access to standing lipid membranes, while controlling membrane voltage and recording electrical signals using micropatterned electrodes. This device provides a powerful tool for combined electrical-optical studies of membranes and membrane proteins.
Article
Engineering, Biomedical
Mohammad Amayreh, Gerhard Baaken, Jan C. Behrends, Yiannos Manoli
IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
(2019)