Article
Medicine, General & Internal
Reza Jalli, Davood Mehrabani, Shahrokh Zare, Mahdi Saeedi Moghadam, Iman Jamhiri, Navid Manafi, Golshid Mehrabani, Janan Ghabanchi, Iman Razeghian Jahromi, Aghdass Rasouli-Nia, Feridoun Karimi-Busheri
Summary: This study assessed the tracking of iron oxide-labeled stem cells transfected by lipofectamine using magnetic resonance imaging (MRI) in vitro and in vivo. Rat dental pulp stem cells (DPSCs) were characterized, labeled with iron oxide nanoparticles, and then transfected with lipofectamine. The internalization of the nanoparticles was confirmed by Prussian blue staining and MRI tracking.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Murilo M. Garrigos, Fernando A. Oliveira, Mariana P. Nucci, Javier B. Mamani, Olivia F. M. Dias, Gabriel N. A. Rego, Mara S. Junqueira, Cicero J. S. Costa, Lucas R. R. Silva, Arielly H. Alves, Nicole M. E. Valle, Luciana Marti, Lionel F. Gamarra
Summary: Bone marrow transplantation is a treatment for various diseases, and this study proposes a new method to evaluate the engraftment and behavior of hematopoietic stem cells. The method involves the use of bioluminescence imaging and inductively coupled plasma mass spectrometry associated with superparamagnetic iron oxide nanoparticles. The study found that the administration of Fluorouracil enriched the population of hematopoietic stem cells in the bone marrow, and labeling the cells with nanoparticles provided information on their internalization status. The study also utilized blood count and bioluminescence imaging to monitor the reconstitution of hematopoiesis and evaluate the transplantation effectiveness.
Article
Biochemistry & Molecular Biology
Edward J. Calabrese
Summary: This paper provides a thorough assessment of hormetic dose responses in embryonic stem cells, suggesting that this response may serve as a fundamental and highly conserved evolutionary strategy.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Biotechnology & Applied Microbiology
Min Wang, Yi Guo, Zexing Deng, Peng Xu
Summary: In this paper, a novel organic-inorganic composite hydrogel was designed, which exhibited excellent mechanical properties and promoted stem cell osteogenic differentiation, showing potential application in bone tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Camila Vazquez Echegaray, Brianne Salvati, Sophie Dulhoste Vivien, Marcos Gabriel Francia, Claudia M. Solari, Maria Soledad Cosentino, Alejandra Guberman, Mercedes Perullini
Summary: This study focuses on the application of mesoporous silica nanoparticles (MSNs) in modulating drug release. By employing a dual strategy of multimodal pore channels and chitosan coating, controlled release of drugs was achieved. The results demonstrate the potential use of MSNs for controlled drug delivery in different cell types, with promising applications in improving stem cell differentiation and reprogramming.
JOURNAL OF BIOTECHNOLOGY
(2022)
Article
Cell Biology
Tahereh Foroutan, Mohammad Zaman Kassaee, Mahdi Salari, Fatemeh Ahmady, Fatemeh Molavi, Fariborz Moayer
Summary: The study found that the use of MGO enhanced the effect of ESC-MSC-CM on reducing necrosis, inflammation, aspartate transaminase, alanine aminotransferase, and alkaline phosphatase in CCl4-induced rat liver failure. Treatment with MGO also upregulated the expression of vascular endothelial growth factor and matrix metalloproteinase-9 (MMP-9). Additionally, ESC-MSC-CM was more efficient compared to MSC-CM in protecting against acute liver damage.
CELL PROLIFERATION
(2021)
Article
Cell Biology
Junmo Wu, Yu Kang, Xiang Luo, Shaoxing Dai, Yuxi Shi, Zhuoyao Li, Zengli Tang, Zhenzhen Chen, Ran Zhu, Pengpeng Yang, Zifan Li, Hong Wang, Xinglong Chen, Ziyi Zhao, Weizhi Ji, Yuyu Niu
Summary: This study presents a chemically defined, xeno-free culture system for culturing and deriving monkey pluripotent stem cells (PSCs) and demonstrates the ability to integrate bioluminescent reporter genes into monkey PSCs and track them in chimeric embryos in vivo and in vitro. The findings have significant implications for primate stem cell engineering and the use of chimeric monkey models in clinical studies.
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Tingting Gao, Xiyuan Zhao, Jie Hao, Yao Tian, Huike Ma, Wenjing Liu, Bin An, Faguo Sun, Shasha Liu, Baojie Guo, Shuaishuai Niu, Zhongwen Li, Chenxin Wang, Yukai Wang, Guihai Feng, Liu Wang, Wei Li, Jun Wu, Meijin Guo, Qi Zhou, Qi Gu
Summary: In this study, a new microcarrier with hyaluronic acid (HA) as the core material was developed for scalable serum-free suspension culture of hESC-derived MSCs. The optimal microcarriers, with a coating collagen concentration of 100 μg/mL or concave-structured surface (cHAMCs), allowed for the expansion of IMRCs within six days with a high yield. The harvested cells showed high viability, immunomodulatory and regenerative therapeutic promise, as well as increased secretion of extracellular matrix (ECM).
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Multidisciplinary
Mungyo Jung, Hyeongseop Kim, Jung Won Hwang, Yejoo Choi, Mikyung Kang, Cheesue Kim, Jihye Hong, Na Kyung Lee, Sangjun Moon, Jong Wook Chang, Suk-joo Choi, Soo-young Oh, Byung-Soo Kim, Duk L. Na, Hyemin Jang
Summary: Alzheimer's disease (AD) is a complex neurodegenerative disease with limited treatment options. Mesenchymal stem cell (MSC) therapy, with its ability to target multiple pathological mechanisms, has recently emerged as a potential novel treatment. However, the low brain retention efficiency of MSCs and the limited therapeutic abilities of autologous MSCs pose challenges to their effectiveness. In this study, we developed iron oxide nanoparticle (IONP)-incorporated human Wharton's jelly-derived MSCs (MSC-IONPs) to enhance their therapeutic potential. With the use of magnetic guidance, MSC-IONPs showed higher brain retention efficiency, leading to improved therapeutic efficacy in murine models of AD. Furthermore, allogeneic MSCs derived from human Wharton's jelly may offer superior therapeutic abilities compared to autologous MSCs in aged AD patients. This study provides a promising strategy for the development of MSC therapies for AD.
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Article
Cell Biology
Chenze Xu, Ali Mohsin, Yanxia Luo, Lili Xie, Yan Peng, Qizheng Wang, Waqas Ahmed, Haifeng Hang, Yingping Zhuang, Meijin Guo
Summary: In this study, non-genetically modified Sertoli-like cells were successfully induced from mouse ESCs using defined protein factors, showing similar characteristics and functionality to natural Sertoli cells. However, the efficiency of the non-GM method was still lower compared to lentiviral transduction. This work lays a foundation for the future production of non-GM Sertoli-like cells for clinical applications and fundamental theory research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Jaisan Islam, Kyoung Ha So, K. C. Elina, Hyeong Cheol Moon, Aryun Kim, Sang Hwan Hyun, Soochong Kim, Young Seok Park
Summary: Transplantation of hESCs showed significant improvement in motor dysfunction in Huntington's disease rat model, with SPION-labeled hESCs migrating to degenerated area and expressing neural markers. This suggests that hESCs transplantation could be a potential treatment for motor dysfunction in Huntington's disease.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Nanoscience & Nanotechnology
Baojie Guo, Yongchao Duan, Zhongwen Li, Yao Tian, Xuedi Cheng, Chunxu Liang, Wenjing Liu, Bin An, Wumei Wei, Tingting Gao, Shasha Liu, Xiyuan Zhao, Shuaishuai Niu, Chenxin Wang, Yukai Wang, Liu Wang, Guihai Feng, Wei Li, Jie Hao, Qi Gu, Qi Zhou, Jun Wu
Summary: The researchers developed a 21-day long-term serum-free culture system and successfully created cell sheets (CSs) with human embryonic stem cell-derived immunity-and-matrix-regulatory cells (IMRCs) that have stronger mechanical properties and greater extracellular matrix secretion capacity. These IMRC-CSs were effective in promoting corneal chemical injury repair and could be transformed into injectable temperature-sensitive hydrogels for uterine adhesion repair through a decellularization process.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Haseeb A. A. Khan, Yong-Kyu Lee, Mohammed Rafi Shaik, Sara T. T. Alrashood, Aishah A. A. Ekhzaimy
Summary: The study synthesized NDG-Mn3O4 nanocomposite and tested its performance in photodynamic therapy (PDT) and magnetic resonance imaging (MRI) for cancer treatment. The results showed that NDG-Mn3O4 nanocomposite exhibited significant cytotoxicity in cancer cells under laser irradiation and increased generation of singlet oxygen proportional to the duration of laser irradiation. It also showed concentration-dependent enhancement of signal intensity in MRI. These findings suggest the potential application of NDG-Mn3O4 nanocomposites in cancer theranostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Multidisciplinary Sciences
Shan Jiang, Xiang Wu, Nicholas J. Rommelfanger, Zihao Ou, Guosong Hong
Summary: This review article provides an overview of recent advances in optical neuromodulation and its applications in addressing physical constraints of in vivo light delivery. The article presents the latest optical techniques for neuromodulation and explores new methods for minimal invasiveness and footprint.
NATIONAL SCIENCE REVIEW
(2022)
Article
Chemistry, Multidisciplinary
Jung Min Lee, Dingchang Lin, Guosong Hong, Kyoung-Ho Kim, Hong-Gyu Park, Charles M. Lieber
Summary: This study demonstrates the high-performance neural recording ability of double-sided three-dimensional (3D) electrodes, which show significant improvements in neuron quantity, spike amplitude, and signal-to-noise ratio compared to standard two-dimensional electrodes. These 3D electrodes also enable stable detection of single-neuron activity over extended periods of time.
Article
Nanoscience & Nanotechnology
Nicholas J. Rommelfanger, Kenneth Brinson, John E. Bailey, Analiese M. Bancroft, Zihao Ou, Guosong Hong
Summary: This study investigates the use of pristine carbon nanotubes as radio frequency absorbers to achieve differential heating in biological tissue, while minimizing nonspecific heating. The results show that the pristine carbon nanotubes can efficiently absorb radio frequencies, providing region-specific heating.
Article
Engineering, Biomedical
Xiang Wu, Yuyan Jiang, Nicholas J. Rommelfanger, Fan Yang, Qi Zhou, Rongkang Yin, Junlang Liu, Sa Cai, Wei Ren, Andrew Shin, Kyrstyn S. Ong, Kanyi Pu, Guosong Hong
Summary: Using wide-field illumination in the second near-infrared spectral window (NIR-II), deep-brain stimulation without implants or tethered optical fibers was accomplished in freely behaving mice. This method can potentially facilitate social behavioral studies in small animals.
NATURE BIOMEDICAL ENGINEERING
(2022)
Editorial Material
Multidisciplinary Sciences
Shan Jiang, Guosong Hong
Article
Multidisciplinary Sciences
Fan Yang, Xiang Wu, Han Cui, Zihao Ou, Shan Jiang, Sa Cai, Qi Zhou, Bryce G. Wong, Hans Huang, Guosong Hong
Summary: The study used a bioinspired demineralization (BID) strategy to synthesize stable colloidal solutions, which can be used for multi-color luminescent tagging applications in vivo.
Article
Chemistry, Multidisciplinary
Fan Yang, Xiang Wu, Han Cui, Shan Jiang, Zihao Ou, Sa Cai, Guosong Hong
Summary: Mechanoluminescent fluids with stable colloidal solutions containing bright emitting nanocrystals have been synthesized using a suppressed dissolution approach. These rechargeable fluids can store photon energy in a reversible manner and have the potential to provide light in tissues for biological applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Wenliang Wang, Xiang Wu, Kai Wing Kevin Tang, Ilya Pyatnitskiy, Rayna Taniguchi, Peter Lin, Richard Zhou, Sam Lander C. Capocyan, Guosong Hong, Huiliang Wang
Summary: Researchers have developed nanoparticles that can release photons when stimulated by sound waves, enabling noninvasive optogenetics with high spatiotemporal resolution. This study allows for the study of cell-type specific neurons in neuroscience and has potential clinical applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Fan Yang, Han Cui, Xiang Wu, Seong-Jong Kim, Guosong Hong
Summary: Ultrasound can deliver energy and control devices wirelessly and non-contactly, and it can propagate and focus in various mediums including biological tissue. Mechanoluminescent (ML) nanocrystal colloids activated by ultrasound provide a wireless method to remotely control a light source, such as a multicolor display. In this study, we synthesized ML colloids with bright blue emission under ultrasound using a biomineral-inspired suppressed dissolution approach, achieving small sizes down to 20 nm. By leveraging the bandgap engineering strategy of all-inorganic perovskite quantum dots (PQDs), we achieved wavelength tunability of the mechanoluminescence of ML colloid/PQD composites. The ultrasound-activated emission of the ML colloid/PQD composites exhibited a highly saturated color gamut covering the entire visible spectrum, enabling the demonstration of a flexible and wireless multicolor display with individually addressed pixels using scanning focused ultrasound.
Article
Chemistry, Multidisciplinary
Xiang Wu, Fan Yang, Sa Cai, Kanyi Pu, Guosong Hong
Summary: The NIR-II window, ranging from 1000 to 1700 nm in wavelength, allows for deep-penetration fluorescent imaging in biological tissues due to reduced light scattering. Recently, deep-brain neuromodulation has been achieved in the NIR-II window using nanotransducers that convert NIR-II light into heat. This Perspective discusses the principles, potential applications, advantages, and limitations of this NIR-II deep-brain neuromodulation technique, as well as future directions for advancement.
Review
Pharmacology & Pharmacy
Fan Yang, Seong-Jong Kim, Xiang Wu, Han Cui, Sei Kwang Hahn, Guosong Hong
Summary: Optogenetics has revolutionized neuroscience research by precisely activating specific neurons using light. However, limited penetration of visible light in neural tissue has been a challenge. Sono-optogenetics solves this problem by using ultrasound and mechano-luminescent nanotransducers for precise light production in three-dimensional neural tissue. This article provides a comprehensive review of sono-optogenetics, discussing its physical principles, emerging applications, and potential transformative impact on neuroscience research.
ADVANCED DRUG DELIVERY REVIEWS
(2023)
Editorial Material
Pharmacology & Pharmacy
Seong-Jong Kim, Gibum Lee, Guosong Hong, Seok Hyun Yun, Sei Kwang Hahn
ADVANCED DRUG DELIVERY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Yichi Su, Joel R. R. Walker, Mary P. P. Hall, Mark A. A. Klein, Xiang Wu, Lance P. P. Encell, Kerriann M. M. Casey, Lan Xiang Liu, Guosong Hong, Michael Z. Z. Lin, Thomas A. A. Kirkland
Summary: Bioluminescence imaging (BLI) is an essential technique in biomedical research that allows non-invasive visualization of cells and biochemical events in living organisms. However, BLI in the central nervous system faces challenges due to the poor performance of luciferases and existing substrates. In this study, researchers discovered a NanoLuc substrate, cephalofurimazine (CFz), which improves the brain performance of BLI. CFz paired with Antares luciferase produces significantly higher signals in the brain compared to the standard combination of d-luciferin and firefly luciferase. This discovery enables high sensitivity imaging of the brain using NanoLuc-based indicators.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biochemical Research Methods
Xiang Wu, Guosong Hong
Summary: This article presents a protocol for deep brain stimulation in freely behaving mice using through-scalp wide-field illumination in the second near-infrared window (NIR-II). The article describes the injection of TRPV1-expressing viruses and macromolecular infrared nanotransducers for deep brain stimulation (MINDS) and details NIR-II neuromodulation in a conditioned place preference test, followed by immunohistochemical studies. This approach is particularly valuable for tether-free deep brain stimulation in social interacting experiments involving multiple subjects. For more information on the use and execution of this protocol, please refer to Wu et al. (2022).
Article
Engineering, Electrical & Electronic
Xiang Wu, Fan Yang, Sa Cai, Guosong Hong
Summary: Light is widely used in life science for controlling and observing biological processes, but limited penetration depth of visible light has been a challenge for using light inside tissue. In the past decade, non-invasive deep-tissue light sources based on luminescent nanomaterials have been developed using photonics and materials science tools. This perspective provides an overview of intravital nanoscopic light sources, discussing their advantages over existing methods for in vivo light delivery, and highlighting their recent applications in optogenetics neuromodulation and fluorescent imaging in live animals. The feasibility of combining these non-invasive light sources with other modalities to expand the utilities of light in biology is also discussed.
PROGRESS IN ELECTROMAGNETICS RESEARCH-PIER
(2023)
