Journal
SMALL
Volume 8, Issue 22, Pages 3510-3516Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201200649
Keywords
carbon nanotubes; periplasmic-binding proteins; glucose; fluorescence
Categories
Funding
- Deshpande Center for Technological Innovation
- Sanofi-Aventis
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1213622] Funding Source: National Science Foundation
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Understanding the structure and function of glucose binding proteins (GBP) complexed with single walled carbon nanotubes (SWNTs) is important for the development of applications including fluorescent sensors and nanostructure particle tracking. Herein, circular dichroism (CD), thermal denaturation, photo-absorption spectroscopy and atomic force microscopy are used to study these nanostructures. The protein retains its glucose-binding activity after complexation and is thermally stable below 36 degrees C. However, the SWNT lowers the midpoint denaturation temperature (T-m) by 5 degrees C and 4 degrees C in the absence and presence of 10 mM glucose, respectively. This data highlights that using techniques such as CD and thermal denaturation may be necessary to fully characterize such protein-nanomaterial nanostructures.
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