Article
Chemistry, Multidisciplinary
Meghan Rothenbroker, Erin M. McConnell, Jimmy Gu, Malene L. Urbanus, Sahar Esmaeili Samani, Alex W. Ensminger, Carlos D. M. Filipe, Yingfu Li
Summary: LP1 is an RNA-cleaving fluorogenic DNAzyme that is reactive with multiple infectious isolates of L. pneumophila, capable of generating a detectable signal for bacterial detection. It holds potential for the development of biosensors to monitor the contamination of L. pneumophila in exposure sources such as cooling towers.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Review
Chemistry, Analytical
Lin Zhu, Yong Chang, Yingying Li, Mingyi Qiao, Lin Liu
Summary: Molecular immobilization and recognition are crucial for biosensor development, and common methods include covalent coupling reactions and non-covalent interactions. NTA ligands are commonly used to chelate metal ions, and their complexes have high affinity for hexahistidine tags. These complexes are widely used for protein separation and immobilization in diagnostic applications. This review focuses on the development of biosensors using NTA-metal complexes as binding units, including techniques such as surface plasmon resonance, electrochemistry, fluorescence, colorimetry, and chemiluminescence.
Review
Biochemistry & Molecular Biology
Yuanbin Wu, Xuning Wang, Meng Zhang, Dongdong Wu
Summary: Gliomas, the most common primary central nervous system tumors, have a high mortality rate. Early and accurate diagnosis of gliomas is crucial, and biosensors can play a significant role in detecting molecular biomarkers for successful treatment. This review explores important molecular biomarkers and various biosensors designed for glioma diagnosis, including electrochemical and optical biosensors. Challenges are discussed, and the review aims to inspire improvements in biosensors.
Review
Chemistry, Multidisciplinary
Julia Ackermann, Justus T. Metternich, Svenja Herbertz, Sebastian Kruss
Summary: Biosensors are important tools in modern basic research and biomedical diagnostics. NIR fluorescent molecular sensors based on SWCNTs have great potential in analyte detection, and the research on chemical design strategies and molecular recognition contributes to their further development.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Spectroscopy
Linus Pauling F. Peixoto, Jacqueline F. L. Santos, Gustavo F. S. Andrade
Summary: In this study, a simple and fast analysis of albumin antibody was achieved using an immuno-biosensor based on gold nanorods and surface-enhanced fluorescence. The results demonstrated the efficiency and specificity of the biosensor, with the signal intensity in the presence of anti-BSA being three times stronger compared to the absence of analyte.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Xinyu Song, Jiabin Lu, Xuanying Zhu, Jiaxin Xu, Yin Jia, Meipin Liu, Lanqin Tang, Lin Sun, Ruiyu Jiang, Lei Zhang
Summary: In this study, a chromogenic molecular switch with a phenol-phthalein backbone was constructed to visually recognize acetate in pure water. The molecular switch receptor showed good selectivity and binding towards acetate, and the mechanism of acetate recognition was found to involve hydrogen bonding and proton transfer. These findings are expected to contribute to the development of a new class of aqueous anion receptors and molecular recognition mechanisms.
Review
Chemistry, Analytical
Daisuke Umeno, Yuki Kimura, Shigeko Kawai-Noma
Summary: Genetically encoded biosensors have the ability to evolve, particularly transcription factor-based biosensors which can be evolved through directed evolution experiments demonstrating their evolvability based on physicochemical properties.
ANALYTICAL SCIENCES
(2021)
Review
Chemistry, Analytical
Lin Liu, Xiaohua Ma, Yong Chang, Hang Guo, Wenqing Wang
Summary: Sensitive and accurate detection of cis-diol-containing biologically related substances is crucial in the fields of metabolomics, glycomics, and proteomics. Boronic acid-based materials have unique properties that allow them to specifically and reversibly interact with 1,2- or 1,3-diols, making them suitable for recognition and detection of cis-diol-containing species. This review summarizes recent advances in the use of boronic acid-based materials as recognition elements and signal labels for the detection of cis-diol-containing biological species, including ribonucleic acids, glycans, glycoproteins, bacteria, exosomes, and tumor cells. The challenges and future perspectives for developing versatile boronic acid-based materials with various applications are also discussed.
Article
Biophysics
P. Clement, J. Ackermann, N. Sahin-Solmaz, S. Herbertz, G. Boero, S. Kruss, J. Brugger
Summary: In this study, the electrical and optical signal transductions of nanoscale biosensors based on single-walled carbon nanotubes (SWCNTs) were compared. The results showed that the combination of SWCNT-based electrical and optical transductions holds great potential for selective detection of important biomolecules, demonstrating its applicability in next generation portable diagnostic assays.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Junyi Chen, Briana L. Hickey, Ziting Gao, Alexie Andrea P. Raz, Richard J. Hooley, Wenwan Zhong
Summary: An arrayed host:guest fluorescence sensor system can differentiate DNA G-quadruplex structures with minor oxidation or methylation modifications in the guanine base, which cannot be detected by circular dichroism (CD). This sensor system can function in diluted serum and is capable of distinguishing individual modifications in DNA mixtures, providing a powerful method for detecting folding changes caused by DNA damage.
Article
Materials Science, Multidisciplinary
Zhaohui Zong, Zhaozhen Cao, Aiyou Hao, Pengyao Xing
Summary: This study reveals the crucial role of water in the functional properties of chiral molecular systems, proposing a hydration-driven chiral molecular rotor system. The impact of water on the chiral molecular movement behavior is discussed, highlighting the potential for fabricating external field-responsive chiral materials.
JOURNAL OF MATERIALS CHEMISTRY C
(2021)
Article
Chemistry, Analytical
Shiting Gu, Xiao-Mei Shi, Di Zhang, Gao-Chao Fan, Xiliang Luo
Summary: An advanced peptide-based photocathodic biosensor integrating a recognition peptide with an antifouling peptide in one probe electrode was developed for accurate detection in complex biological matrixes. This biosensor demonstrated excellent anti-interference to nonspecific proteins and reducing agents due to the antifouling peptide, and showed good sensitivity utilizing the recognition peptide. This peptide-integrated method provides a new perspective for practical applications of photocathodic biosensors.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Paula Mayorga-Burrezo, Jose Munoz, Dagmar Zaoralova, Michal Otyepka, Martin Pumera
Summary: The development of versatile bio-inspired 2D-MXenes using molecular engineering allows for the creation of highly sensitive sensing platforms with excellent performance transfer and intriguing potential applications.
Article
Biochemistry & Molecular Biology
Yana Bodnar, Christopher Horst Lillig
Summary: Redox modifications play a regulatory role in key enzymes and signal-transducing proteins by modifying specific cysteinyl and methionyl residues. Structural analysis of protein data in the RCSB protein data bank reveals that the conformational changes induced by redox modifications are small and not strictly dependent on solvent accessibility. Certain amino acid residues, such as histidyl and tyrosyl, appear to be enriched in the vicinity of susceptible residues, providing potential predictive factors for redox modifications.
Review
Chemistry, Multidisciplinary
Anirban Mondal, Ryojun Toyoda, Romain Costil, Ben L. Feringa
Summary: This article summarizes the design, synthesis, and dynamic properties of molecular machines, and presents methods for controlled switching or rotation of different classes of atropisomers under chemical stimulus, categorizing them as molecular switches, rotors, motors, and autonomous motors. Future perspectives and challenges for building sophisticated molecular machines are also provided.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Robert J. Hamers, Desiree M. Bates, Kristopher Jose Aguayo Barragan, Danica G. Gressel, Beau S. Schweitzer, Jairo Villalona, Cheri A. Barta, Judith Burstyn, Andrew E. Greenberg, Michael P. Schwartz
Summary: This study describes programs at the University of Wisconsin-Madison Department of Chemistry aimed at increasing representation and improving outcomes for graduate students from underrepresented minority groups. The experiences have yielded both successes and failures, providing direction for future improvements and efforts.
JOURNAL OF CHEMICAL EDUCATION
(2022)
Article
Chemistry, Multidisciplinary
Fangjia Li, Hugh D. Mitchell, Arielle C. Mensch, Dehong Hu, Elizabeth D. Laudadio, Jenny K. Hedlund Orbeck, Robert J. Hamers, Galya Orr
Summary: This study utilized single-cell analysis to investigate individual cell responses to lithium cobalt oxide nanoparticles, revealing significant variability in gene expression levels between neighboring cells under the same exposure conditions, as well as a prioritization of gene expression for restoring energy balance in individual cells.
Article
Nanoscience & Nanotechnology
Becky J. Curtis, Nicholas J. Niemuth, Evan Bennett, Angela Schmoldt, Olaf Mueller, Aurash A. Mohaimani, Elizabeth D. Laudadio, Yu Shen, Jason C. White, Robert J. Hamers, Rebecca D. Klaper
Summary: The physico-chemical characteristics of engineered nanomaterials play a crucial role in their impact on organisms, but the sensitivity of different species also contributes to the effects. Various factors, such as differences in biochemical responses, exposure, and uptake, may cause the sensitivity to vary significantly across species. A comparison of three model species revealed that their different sensitivities to lithium cobalt oxide nanosheets can be attributed to variations in immune-response, iron-sulfur protein, and central nervous system pathways. Nanomaterial uptake and dissolution do not fully explain the differences between species.
NATURE NANOTECHNOLOGY
(2022)
Correction
Nanoscience & Nanotechnology
Becky J. Curtis, Nicholas J. Niemuth, Evan Bennett, Angela Schmoldt, Olaf Mueller, Aurash A. Mohaimani, Elizabeth D. Laudadio, Yu Shen, Jason C. White, Robert J. Hamers, Rebecca D. Klaper
NATURE NANOTECHNOLOGY
(2022)
Editorial Material
Chemistry, Multidisciplinary
Anke Krueger, Nianjun Yang, Robert J. Hamers
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Electrochemistry
L. V. Morris, C. A. Ortiz-Ledon, R. J. Hamers
Summary: A new class of organosilicon additives was introduced to explore their effect on the electrochemistry of Si anode surfaces during the first cycle. EQCM and EIS were used to investigate the impedance of the cells and XPS was used to analyze the composition of the SEI layers. The results showed that the organosilicon-treated cells had an increase in impedance and thinner SEI layers richer in LiF and with less organic material.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Nanoscience & Nanotechnology
Paige C. Kinsley, Curtis M. Green, Jaya Borgatta, Catherine E. Kruszynski Earl, Elizabeth D. Laudadio, Robert J. Hamers
Summary: We have developed a method to control the behavior of metal oxide nanoparticles in aqueous media by producing nanometer-thin carbon shells on their surface, allowing subsequent covalent chemical functionalization with molecular ligands. This approach enables the nanoparticles to acquire specific molecular properties in aqueous media, utilizing the strong and stable C-C interfacial bonds between the surface ligands and the carbon shell.
ACS APPLIED NANO MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Deepti Sharan, Daniel Wolfson, Curtis M. Green, Paul Lemke, Alessandra G. Gavin, Robert J. Hamers, Z. Vivian Feng, Erin E. Carlson
Summary: The use of complex metal oxide nanoparticles has increased significantly in recent years, particularly in electric vehicle batteries. However, our understanding of their impact on environmental organisms has not kept pace with their usage. This study examines the effects of chronic exposure to nanoscale lithiated nickel manganese cobalt oxide (NMC) on the bacterium Shewanella oneidensis. The results show that chronic exposure to NMC leads to genetic-level variation in the bacteria, including the production of reactive oxygen species (ROS) and DNA damage.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Multidisciplinary
Shreyasi Sengupta, Swapnil B. Ambade, Tana L. O'Keefe, Falak Tawakalna, Jenny K. Hedlund Orbeck, Robert J. Hamers, Z. Vivian Feng, Christy L. Haynes, Zeev Rosenzweig
Summary: This study investigates the environmental fate of InSe nanosheets and the impact of EGCG-coated nanosheets on bacterial growth.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Multidisciplinary
Thomas R. Kuech, Nasim Ganji, Caroline Anastasia, Marco D. Torelli, Eric S. Melby, Arielle C. Mensch, Emily R. Caudill, Ralf Zimmermann, Robert J. Hamers, Joel A. Pedersen
Summary: The interactions between cationic polymer-wrapped diamond nanoparticles (PAH-DNPs) and lipid bilayers are influenced by the phospholipid composition and surface charge. Electrostatic attractions and repulsions play key roles in the initial attachment and final extent of nanoparticle adhesion. The formation of a lipid corona on PAH-DNPs reduces the effective charge density and affects the adhesion extent. The inclusion of charged phospholipids decreases the attachment efficiency and extent by reducing the electrostatic attraction between PAH-DNPs and the bilayers.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Multidisciplinary
Christian A. Reardon-Lochbaum, Ravithree D. Senanayake, Rocio Amaro Marquez, Kha Trinh, Khoi Nguyen L. Hoang, Tobias Rangel Guillen, Catherine J. Murphy, Robert J. Hamers, Joel A. Pedersen, Rigoberto Hernandez
Summary: Supported lipid bilayers are commonly used as model systems for studying interactions between biological membranes and proteins or nanoparticles. The choice of substrate material, such as gold or SiO2, influences the interactions and adsorption behavior of anionic nanoparticles and cytochrome c to the bilayers. The underlying substrate material plays a role in determining the extent of interaction between nanoscale analytes and supported lipid bilayers.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Article
Chemistry, Multidisciplinary
Dongyue Liang, Juan Liu, Hendrik Heinz, Sara E. Mason, Robert J. Hamers, Qiang Cui
Summary: A classical model has been developed for studying the LiCoO2 (LCO) (001)/water interface, which provides reliable descriptions of interfacial properties such as the density distribution of water molecules and the adsorption behavior of molecules like NADH.
Article
Chemistry, Physical
Shuo Li, Luca Francaviglia, Daniel D. Kohler, Zachary R. Jones, Eric T. Zhao, D. Frank Ogletree, Alexander Weber-Bargioni, Nicholas A. Melosh, Robert J. Hamers
Summary: This study investigates highly stable silicon-vacancy (SiV) centers in diamond for sensing and quantum information science. The results show that complete encapsulation of Ag nanospheres with SiV centers in a thin diamond coating leads to hybrid core-shell nanostructures with extraordinary chemical and thermal stability and enhanced optical properties. The stability and optical properties of these Ag-diamond core-shell structures make them promising candidates for high-efficiency imaging and quantum-based sensing applications.
Article
Chemistry, Multidisciplinary
Austin H. Henke, Elizabeth D. Laudadio, Jenny K. Hedlund Orbeck, Ali Abbaspour Tamijani, Khoi Nguyen L. Hoang, Sara E. Mason, Catherine J. Murphy, Z. Vivian Feng, Robert J. Hamers
Summary: The interaction of LiCoO2 nanoparticles with NADH and GSH induces the release of Co2+ ions and alters the redox state of these biologically important transporters, demonstrating the potential biological impacts of high-valence metal oxide nanomaterials.
ENVIRONMENTAL SCIENCE-NANO
(2021)
Article
Chemistry, Multidisciplinary
Metti K. Gari, Paul Lemke, Kelly H. Lu, Elizabeth D. Laudadio, Austin H. Henke, Curtis M. Green, Thomas Pho, Khoi Nguyen L. Hoang, Catherine J. Murphy, Robert J. Hamers, Z. Vivian Feng
Summary: The study showed that LiCoO2 nanoparticles generated abiotic reactive oxygen species primarily through H2O2 production when freshly suspended. As the suspension aged, H2O2 generation decreased while the release of Co ions increased, leading to DNA damage and changes in gene expression in the exposed bacterium B. subtilis. The findings highlight the importance of understanding the dynamic transformation of nanoparticles and their impact on oxidative stress responses in cells.
ENVIRONMENTAL SCIENCE-NANO
(2021)